2bb0 - Revision history

OCA at 13:43, 13 March 2024

2024-03-13T13:43:12Z

← Older revision		Revision as of 13:43, 13 March 2024
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	<StructureSection load='2bb0' size='340' side='right'caption='[[2bb0]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2bb0' size='340' side='right'caption='[[2bb0]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bb0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BB0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BB0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bb0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BB0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BB0 FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>~~, ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https:~~/~~/en.wikipedia.org/wiki/Imidazolonepropionase Imidazolonepropionase]~~, ~~with EC number [https~~:~~//www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.7 3.5.2.7]~~ </~~span~~></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bb0 OCA], [https://pdbe.org/2bb0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bb0 RCSB], [https://www.ebi.ac.uk/pdbsum/2bb0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bb0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bb0 OCA], [https://pdbe.org/2bb0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bb0 RCSB], [https://www.ebi.ac.uk/pdbsum/2bb0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bb0 ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/HUTI_BACSU HUTI_BACSU]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bb0 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bb0 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Imidazolonepropionase (EC 3.5.2.7) catalyzes the third step in the universal histidine degradation pathway, hydrolyzing the carbon-nitrogen bonds in 4-imidazolone-5-propionic acid to yield N-formimino-l-glutamic acid. Here we report the crystal structures of the Bacillus subtilis imidazolonepropionase and its complex at 2.0-A resolution with substrate analog imidazole-4-acetic acid sodium (I4AA). The structure of the native enzyme contains two domains, a TIM (triose-phosphate isomerase) barrel domain with two insertions and a small beta-sandwich domain. The TIM barrel domain is quite similar to the members of the alpha/beta barrel metallo-dependent hydrolase superfamily, especially to Escherichia coli cytosine deaminase. A metal ion was found in the central cavity of the TIM barrel and was tightly coordinated to residues His-80, His-82, His-249, Asp-324, and a water molecule. X-ray fluorescence scan analysis confirmed that the bound metal ion was a zinc ion. An acetate ion, 6 A away from the zinc ion, was also found in the potential active site. In the complex structure with I4AA, a substrate analog, I4AA replaced the acetate ion and contacted with Arg-89, Try-102, Tyr-152, His-185, and Glu-252, further defining and confirming the active site. The detailed structural studies allowed us to propose a zinc-activated nucleophilic attack mechanism for the hydrolysis reaction catalyzed by the enzyme.

	A catalytic mechanism revealed by the crystal structures of the imidazolonepropionase from Bacillus subtilis.,Yu Y, Liang YH, Brostromer E, Quan JM, Panjikar S, Dong YH, Su XD J Biol Chem. 2006 Dec 1;281(48):36929-36. Epub 2006 Sep 21. PMID:16990261<ref>PMID:16990261</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2bb0" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Bacillus ~~globigii migula 1900]]~~		[[Category: Bacillus subtilis]]
	~~[[Category: Imidazolonepropionase~~]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Liang~~, Y H~~]]		[[Category: Liang YH]]
	[[Category: Su~~, X D~~]]		[[Category: Su XD]]
	[[Category: Yu, Y]]		[[Category: Yu Y]]
	~~[[Category: Hydrolase]]~~
	~~[[Category: Tim barrel~~]]

OCA at 09:43, 5 May 2021

2021-05-05T09:43:34Z

← Older revision		Revision as of 09:43, 5 May 2021
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	<StructureSection load='2bb0' size='340' side='right'caption='[[2bb0]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2bb0' size='340' side='right'caption='[[2bb0]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bb0]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BB0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2BB0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bb0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BB0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BB0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Imidazolonepropionase Imidazolonepropionase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.7 3.5.2.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Imidazolonepropionase Imidazolonepropionase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.7 3.5.2.7] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2bb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bb0 OCA], [~~http~~://pdbe.org/2bb0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2bb0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2bb0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2bb0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bb0 OCA], [https://pdbe.org/2bb0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bb0 RCSB], [https://www.ebi.ac.uk/pdbsum/2bb0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bb0 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 09:25, 24 July 2019

2019-07-24T09:25:00Z

← Older revision		Revision as of 09:25, 24 July 2019
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	==Structure of Imidazolonepropionase from Bacillus subtilis==		==Structure of Imidazolonepropionase from Bacillus subtilis==
	<StructureSection load='2bb0' size='340' side='right' caption='[[2bb0]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2bb0' size='340' side='right'caption='[[2bb0]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bb0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BB0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bb0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BB0 FirstGlance]. <br>
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bb/2bb0_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bb/2bb0_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	[[Category: Bacillus globigii migula 1900]]		[[Category: Bacillus globigii migula 1900]]
	[[Category: Imidazolonepropionase]]		[[Category: Imidazolonepropionase]]
			[[Category: Large Structures]]
	[[Category: Liang, Y H]]		[[Category: Liang, Y H]]
	[[Category: Su, X D]]		[[Category: Su, X D]]

OCA at 11:29, 12 October 2017

2017-10-12T11:29:46Z

← Older revision		Revision as of 11:29, 12 October 2017
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	==Structure of Imidazolonepropionase from Bacillus subtilis==		==Structure of Imidazolonepropionase from Bacillus subtilis==
	<StructureSection load='2bb0' size='340' side='right' caption='[[2bb0]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2bb0' size='340' side='right' caption='[[2bb0]], [[Resolution\|resolution]] 2.00Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Imidazolonepropionase Imidazolonepropionase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.7 3.5.2.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Imidazolonepropionase Imidazolonepropionase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.7 3.5.2.7] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bb0 OCA], [http://pdbe.org/2bb0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bb0 RCSB], [http://www.ebi.ac.uk/pdbsum/2bb0 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bb0 OCA], [http://pdbe.org/2bb0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bb0 RCSB], [http://www.ebi.ac.uk/pdbsum/2bb0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2bb0 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 13:29, 7 February 2016

2016-02-07T13:29:51Z

← Older revision		Revision as of 13:29, 7 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bb0 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 01:54, 10 September 2015

2015-09-10T01:54:56Z

← Older revision		Revision as of 01:54, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2bb0' size='340' side='right' caption='[[2bb0]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2bb0' size='340' side='right' caption='[[2bb0]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bb0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_subtilis Bacillus subtilis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BB0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bb0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BB0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Imidazolonepropionase Imidazolonepropionase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.7 3.5.2.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Imidazolonepropionase Imidazolonepropionase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.7 3.5.2.7] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bb0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bb0 RCSB], [http://www.ebi.ac.uk/pdbsum/2bb0 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bb0 OCA], [http://pdbe.org/2bb0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bb0 RCSB], [http://www.ebi.ac.uk/pdbsum/2bb0 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2bb0" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Bacillus ~~subtilis~~]]		[[Category: Bacillus globigii migula 1900]]
	[[Category: Imidazolonepropionase]]		[[Category: Imidazolonepropionase]]
	[[Category: Liang, Y H]]		[[Category: Liang, Y H]]

OCA at 10:27, 8 January 2015

2015-01-08T10:27:32Z

← Older revision		Revision as of 10:27, 8 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bb0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BB0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bb0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BB0 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Imidazolonepropionase Imidazolonepropionase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.7 3.5.2.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Imidazolonepropionase Imidazolonepropionase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.7 3.5.2.7] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bb0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bb0 RCSB], [http://www.ebi.ac.uk/pdbsum/2bb0 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bb0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bb0 RCSB], [http://www.ebi.ac.uk/pdbsum/2bb0 PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
	[[Category: Imidazolonepropionase]]		[[Category: Imidazolonepropionase]]
	[[Category: Liang, Y H.]]		[[Category: Liang, Y H]]
	[[Category: Su, X D.]]		[[Category: Su, X D]]
	[[Category: Yu, Y.]]		[[Category: Yu, Y]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Tim barrel]]		[[Category: Tim barrel]]

OCA at 01:12, 30 September 2014

2014-09-30T01:12:47Z

← Older revision		Revision as of 01:12, 30 September 2014
Line 1:		Line 1:
	[[Image:~~2bb0~~.~~png~~\|~~left~~\|~~200px~~]]		==Structure of Imidazolonepropionase from Bacillus subtilis==
			<StructureSection load='2bb0' size='340' side='right' caption='[[2bb0]], [[Resolution\|resolution]] 2.00Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2bb0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BB0 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Imidazolonepropionase Imidazolonepropionase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.7 3.5.2.7] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bb0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bb0 RCSB], [http://www.ebi.ac.uk/pdbsum/2bb0 PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bb/2bb0_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Imidazolonepropionase (EC 3.5.2.7) catalyzes the third step in the universal histidine degradation pathway, hydrolyzing the carbon-nitrogen bonds in 4-imidazolone-5-propionic acid to yield N-formimino-l-glutamic acid. Here we report the crystal structures of the Bacillus subtilis imidazolonepropionase and its complex at 2.0-A resolution with substrate analog imidazole-4-acetic acid sodium (I4AA). The structure of the native enzyme contains two domains, a TIM (triose-phosphate isomerase) barrel domain with two insertions and a small beta-sandwich domain. The TIM barrel domain is quite similar to the members of the alpha/beta barrel metallo-dependent hydrolase superfamily, especially to Escherichia coli cytosine deaminase. A metal ion was found in the central cavity of the TIM barrel and was tightly coordinated to residues His-80, His-82, His-249, Asp-324, and a water molecule. X-ray fluorescence scan analysis confirmed that the bound metal ion was a zinc ion. An acetate ion, 6 A away from the zinc ion, was also found in the potential active site. In the complex structure with I4AA, a substrate analog, I4AA replaced the acetate ion and contacted with Arg-89, Try-102, Tyr-152, His-185, and Glu-252, further defining and confirming the active site. The detailed structural studies allowed us to propose a zinc-activated nucleophilic attack mechanism for the hydrolysis reaction catalyzed by the enzyme.

	~~{{STRUCTURE_2bb0\| PDB=2bb0 \| SCENE= }}~~		A catalytic mechanism revealed by the crystal structures of the imidazolonepropionase from Bacillus subtilis.,Yu Y, Liang YH, Brostromer E, Quan JM, Panjikar S, Dong YH, Su XD J Biol Chem. 2006 Dec 1;281(48):36929-36. Epub 2006 Sep 21. PMID:16990261<ref>PMID:16990261</ref>

	~~===Structure~~ of ~~Imidazolonepropionase from Bacillus subtilis===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_16990261}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2bb0]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_subtilis Bacillus subtilis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2BB0 OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:016990261<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
	[[Category: Imidazolonepropionase]]		[[Category: Imidazolonepropionase]]

OCA: Protected "2bb0" [edit=sysop:move=sysop]

2013-01-06T10:28:23Z

Protected "2bb0" [edit=sysop:move=sysop]

← Older revision	Revision as of 10:28, 6 January 2013
(No difference)

OCA at 10:28, 6 January 2013

2013-01-06T10:28:22Z

← Older revision		Revision as of 10:28, 6 January 2013
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	~~{{Seed}}~~
	[[Image:2bb0.png\|left\|200px]]		[[Image:2bb0.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2bb0", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2bb0\| PDB=2bb0 \| SCENE= }}		{{STRUCTURE_2bb0\| PDB=2bb0 \| SCENE= }}

	===Structure of Imidazolonepropionase from Bacillus subtilis===		===Structure of Imidazolonepropionase from Bacillus subtilis===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_16990261}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 16990261 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_16990261}}		{{ABSTRACT_PUBMED_16990261}}

	==About this Structure==		==About this Structure==
	~~2BB0~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BB0 OCA].		[[2bb0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BB0 OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~16990261~~</ref><references group="xtra"/>		<ref group="xtra">PMID:016990261</ref><references group="xtra"/>
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
	[[Category: Imidazolonepropionase]]		[[Category: Imidazolonepropionase]]
Line 31:		Line 19:
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Tim barrel]]		[[Category: Tim barrel]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 14:07:25 2009''~~