2bea - Revision history

OCA at 09:01, 6 November 2024

2024-11-06T09:01:10Z

← Older revision		Revision as of 09:01, 6 November 2024
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	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/be/2bea_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/be/2bea_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/~~initialview01~~.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>

OCA at 11:21, 22 May 2024

2024-05-22T11:21:17Z

← Older revision		Revision as of 11:21, 22 May 2024
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	<StructureSection load='2bea' size='340' side='right'caption='[[2bea]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='2bea' size='340' side='right'caption='[[2bea]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bea]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Dolichos_tetragonolobus Dolichos~~ tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BEA FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bea]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BEA FirstGlance]. <br>
	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[1eyl\|1eyl]]~~, [[~~1fmz~~\|~~1fmz~~]]~~, [[2beb\|2beb]]</div>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.35Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bea OCA], [https://pdbe.org/2bea PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bea RCSB], [https://www.ebi.ac.uk/pdbsum/2bea PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bea ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bea OCA], [https://pdbe.org/2bea PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bea RCSB], [https://www.ebi.ac.uk/pdbsum/2bea PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bea ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/ICW3_PSOTE ICW3_PSOTE]] Inhibits alpha-chymotrypsin at the molar ratio of 1:2 in state of 1:1.		[https://www.uniprot.org/uniprot/ICW3_PSOTE ICW3_PSOTE] Inhibits alpha-chymotrypsin at the molar ratio of 1:2 in state of 1:1.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Dolichos tetragonolobus]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Dasgupta, J~~]]		[[Category: Psophocarpus tetragonolobus]]
	[[Category: ~~Dattagupta,~~ J K]]		[[Category: Dasgupta J]]
	[[Category: ~~Khamrui, S~~]]		[[Category: Dattagupta JK]]
	[[Category: ~~Sen, U~~]]		[[Category: Khamrui S]]
	[[Category: ~~Beta trefoil]]~~		[[Category: Sen U]]
	~~[[Category: Hydrolase inhibitor]]~~
	~~[[Category: Mutant]]~~
	~~[[Category: Spacer~~]]

OCA at 17:17, 20 October 2021

2021-10-20T17:17:38Z

← Older revision		Revision as of 17:17, 20 October 2021
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	<StructureSection load='2bea' size='340' side='right'caption='[[2bea]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='2bea' size='340' side='right'caption='[[2bea]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bea]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Dolichos_tetragonolobus Dolichos tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2BEA FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bea]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dolichos_tetragonolobus Dolichos tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BEA FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eyl\|1eyl]], [[1fmz\|1fmz]], [[2beb\|2beb]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1eyl\|1eyl]], [[1fmz\|1fmz]], [[2beb\|2beb]]</div></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2bea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bea OCA], [~~http~~://pdbe.org/2bea PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2bea RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2bea PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2bea ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bea OCA], [https://pdbe.org/2bea PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bea RCSB], [https://www.ebi.ac.uk/pdbsum/2bea PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bea ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/ICW3_PSOTE ICW3_PSOTE]] Inhibits alpha-chymotrypsin at the molar ratio of 1:2 in state of 1:1.		[[https://www.uniprot.org/uniprot/ICW3_PSOTE ICW3_PSOTE]] Inhibits alpha-chymotrypsin at the molar ratio of 1:2 in state of 1:1.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:08, 19 February 2020

2020-02-19T08:08:22Z

← Older revision		Revision as of 08:08, 19 February 2020
Line 1:		Line 1:

	==Crystal structure of Asn14 to Gly mutant of WCI==		==Crystal structure of Asn14 to Gly mutant of WCI==
	<StructureSection load='2bea' size='340' side='right' caption='[[2bea]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='2bea' size='340' side='right'caption='[[2bea]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bea]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dolichos_tetragonolobus Dolichos tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEA FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bea]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dolichos_tetragonolobus Dolichos tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEA FirstGlance]. <br>
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	==See Also==		==See Also==
	*[[Chymotrypsin ~~Inhibitor~~\|Chymotrypsin ~~Inhibitor~~]]		*[[Chymotrypsin inhibitor 3D structures\|Chymotrypsin inhibitor 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Dolichos tetragonolobus]]		[[Category: Dolichos tetragonolobus]]
			[[Category: Large Structures]]
	[[Category: Dasgupta, J]]		[[Category: Dasgupta, J]]
	[[Category: Dattagupta, J K]]		[[Category: Dattagupta, J K]]

OCA at 06:34, 16 May 2018

2018-05-16T06:34:48Z

← Older revision		Revision as of 06:34, 16 May 2018
Line 1:		Line 1:

	==Crystal structure of Asn14 to Gly mutant of WCI==		==Crystal structure of Asn14 to Gly mutant of WCI==
	<StructureSection load='2bea' size='340' side='right' caption='[[2bea]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='2bea' size='340' side='right' caption='[[2bea]], [[Resolution\|resolution]] 2.35Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2bea]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dolichos_tetragonolobus Dolichos tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEA FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bea]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dolichos_tetragonolobus Dolichos tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEA FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eyl\|1eyl]], [[1fmz\|1fmz]], [[2beb\|2beb]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eyl\|1eyl]], [[1fmz\|1fmz]], [[2beb\|2beb]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bea OCA], [http://pdbe.org/2bea PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bea RCSB], [http://www.ebi.ac.uk/pdbsum/2bea PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bea OCA], [http://pdbe.org/2bea PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bea RCSB], [http://www.ebi.ac.uk/pdbsum/2bea PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2bea ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/be/2bea_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/be/2bea_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 21:01, 7 February 2016

2016-02-07T21:01:04Z

← Older revision		Revision as of 21:01, 7 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bea ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 04:57, 10 September 2015

2015-09-10T04:57:05Z

← Older revision		Revision as of 04:57, 10 September 2015
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	<StructureSection load='2bea' size='340' side='right' caption='[[2bea]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='2bea' size='340' side='right' caption='[[2bea]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bea]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Psophocarpus_tetragonolobus Psophocarpus~~ tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEA FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bea]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dolichos_tetragonolobus Dolichos tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEA FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eyl\|1eyl]], [[1fmz\|1fmz]], [[2beb\|2beb]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eyl\|1eyl]], [[1fmz\|1fmz]], [[2beb\|2beb]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bea OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bea RCSB], [http://www.ebi.ac.uk/pdbsum/2bea PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bea OCA], [http://pdbe.org/2bea PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bea RCSB], [http://www.ebi.ac.uk/pdbsum/2bea PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2bea" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Psophocarpus~~ tetragonolobus]]		[[Category: Dolichos tetragonolobus]]
	[[Category: Dasgupta, J]]		[[Category: Dasgupta, J]]
	[[Category: Dattagupta, J K]]		[[Category: Dattagupta, J K]]

OCA at 18:13, 24 December 2014

2014-12-24T18:13:22Z

← Older revision		Revision as of 18:13, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bea]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEA FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bea]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEA FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eyl\|1eyl]], [[1fmz\|1fmz]], [[2beb\|2beb]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eyl\|1eyl]], [[1fmz\|1fmz]], [[2beb\|2beb]]</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bea OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bea RCSB], [http://www.ebi.ac.uk/pdbsum/2bea PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bea OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bea RCSB], [http://www.ebi.ac.uk/pdbsum/2bea PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/ICW3_PSOTE ICW3_PSOTE]] Inhibits alpha-chymotrypsin at the molar ratio of 1:2 in state of 1:1.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Psophocarpus tetragonolobus]]		[[Category: Psophocarpus tetragonolobus]]
	[[Category: Dasgupta, J.]]		[[Category: Dasgupta, J]]
	[[Category: Dattagupta, J K.]]		[[Category: Dattagupta, J K]]
	[[Category: Khamrui, S.]]		[[Category: Khamrui, S]]
	[[Category: Sen, U.]]		[[Category: Sen, U]]
	[[Category: Beta trefoil]]		[[Category: Beta trefoil]]
	[[Category: Hydrolase inhibitor]]		[[Category: Hydrolase inhibitor]]
	[[Category: Mutant]]		[[Category: Mutant]]
	[[Category: Spacer]]		[[Category: Spacer]]

OCA at 03:02, 29 September 2014

2014-09-29T03:02:44Z

← Older revision		Revision as of 03:02, 29 September 2014
Line 1:		Line 1:
	[[Image:~~2bea~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of Asn14 to Gly mutant of WCI==
			<StructureSection load='2bea' size='340' side='right' caption='[[2bea]], [[Resolution\|resolution]] 2.35Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2bea]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEA FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eyl\|1eyl]], [[1fmz\|1fmz]], [[2beb\|2beb]]</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bea OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bea RCSB], [http://www.ebi.ac.uk/pdbsum/2bea PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/be/2bea_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The scaffold of serine protease inhibitors plays a significant role in the process of religation which resists proteolysis of the inhibitor in comparison to a substrate. Although the role of the conserved scaffolding Asn residue was previously implicated in the maintenance of the binding loop conformation of Kunitz (STI) inhibitors, its possible involvement in the prevention of proteolysis is still unexplored. In this paper, we have investigated the specific role of the spacer Asn in the prevention of proteolysis through structural and biochemical studies on the mutants where Asn14 of winged bean chymotrypsin inhibitor (WCI) has been replaced by Gly, Ala, Thr, Leu, and Gln. A residue having no side chain or beta-branching at the 14th position creates deformation and insufficient protrusion of the binding loop, and as a result N14G and N14T lose the ability to recognize proteases. Although the reactive site loop conformation of N14A and N14Q are almost identical to WCI, biochemical results present N14A as a substrate indicating that the methyl group of Ala14 is not suitable to capture the cleaved parts together for religation. The poor inhibitory power of N14L points toward the chemical incompatibility of Leu at the 14th position, although its size is the same as Asn; on the other hand, slight loss of inhibitory potency of N14Q is attributed to the inappropriate placement of the Gln14 polar head, caused by the strained accommodation of its bigger side chain. These observations collectively allow us to conclude that the side chain of spacer Asn fits snugly into the concave space of the reactive site loop cavity and its ND2 atom forms hydrogen bonds with the P2 and P1' carbonyl O at either side of the scissile bond holding the cleaved products together for religation. Through database analysis, we have identified such spacer asparagines in five other families of serine protease inhibitors with a similar disposition of their ND2 atoms, which supports our proposition.

	~~{{STRUCTURE_2bea\| PDB=2bea \| SCENE= }}~~		Spacer Asn determines the fate of Kunitz (STI) inhibitors, as revealed by structural and biochemical studies on WCI mutants.,Dasgupta J, Khamrui S, Dattagupta JK, Sen U Biochemistry. 2006 Jun 6;45(22):6783-92. PMID:16734415<ref>PMID:16734415</ref>

	~~===Crystal structure of Asn14 to Gly mutant~~ of ~~WCI===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_16734415}}~~

	~~==About this Structure==~~
	~~[[2bea]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2BEA OCA].~~

	==See Also==		==See Also==
	*[[Chymotrypsin Inhibitor\|Chymotrypsin Inhibitor]]		*[[Chymotrypsin Inhibitor\|Chymotrypsin Inhibitor]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:016734415<~~/~~ref~~><~~ref group="xtra">PMID:016081330</ref><references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Psophocarpus tetragonolobus]]		[[Category: Psophocarpus tetragonolobus]]
	[[Category: Dasgupta, J.]]		[[Category: Dasgupta, J.]]

OCA at 07:51, 20 October 2012

2012-10-20T07:51:18Z

← Older revision		Revision as of 07:51, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[2bea]] is a 2 chain structure ~~of [[Chymotrypsin Inhibitor]]~~ with sequence from [http://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEA OCA].		[[2bea]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEA OCA].

	==See Also==		==See Also==