2bec - Revision history

OCA at 13:43, 13 March 2024

2024-03-13T13:43:25Z

← Older revision		Revision as of 13:43, 13 March 2024
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	<StructureSection load='2bec' size='340' side='right'caption='[[2bec]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2bec' size='340' side='right'caption='[[2bec]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bec]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BEC FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bec]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BEC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=YT3:YTTRIUM+(III)+ION'>YT3</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=YT3:YTTRIUM+(III)+ION'>YT3</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bec OCA], [https://pdbe.org/2bec PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bec RCSB], [https://www.ebi.ac.uk/pdbsum/2bec PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bec ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bec OCA], [https://pdbe.org/2bec PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bec RCSB], [https://www.ebi.ac.uk/pdbsum/2bec PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bec ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/CHP2_HUMAN CHP2_HUMAN]] Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and protecting cells from serum deprivation-induced death. Also plays a role in the regulation of cell proliferation and tumor growth by increasing the phosphatase activity of PPP3CA in a calcium-dependent manner. Activator of the calcineurin/NFAT signaling pathway. Involved in the cytoplasmic translocation of the transcription factor NFATC3 to the nucleus.<ref>PMID:12226101</ref> <ref>PMID:18815128</ref> [[https://www.uniprot.org/uniprot/SL9A1_HUMAN SL9A1_HUMAN]] Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.<ref>PMID:8901634</ref> <ref>PMID:11350981</ref> <ref>PMID:15035633</ref>		[https://www.uniprot.org/uniprot/CHP2_HUMAN CHP2_HUMAN] Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and protecting cells from serum deprivation-induced death. Also plays a role in the regulation of cell proliferation and tumor growth by increasing the phosphatase activity of PPP3CA in a calcium-dependent manner. Activator of the calcineurin/NFAT signaling pathway. Involved in the cytoplasmic translocation of the transcription factor NFATC3 to the nucleus.<ref>PMID:12226101</ref> <ref>PMID:18815128</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bec ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bec ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The plasma membrane Na+/H+ exchangers (NHE) require calcineurin B homologous protein (CHP) as an obligatory binding partner for ion transport. Here, we report the first crystal structure of CHP (CHP2 isoform) in complex with its binding domain in NHE1. We show that the cytoplasmic alpha-helix of NHE1 is inserted into the hydrophobic cleft formed by N- and C-lobes of CHP2 and that the size and shape of this crevice together with hydrogen bond formation at multiple positions assure a high degree of specificity for interaction with NHE members. Structure-based mutagenesis revealed the importance of hydrophobic interactions between CHP/NHE1 for the function of NHE1. Furthermore, the crystal structure shows the existence of a protruding CHP-unique region, and deletion of this region in CHP2 inhibited the NHE1 activity by inducing the acidic shift of intracellular pH dependence, while preserving interaction with NHE1. These findings suggest that CHP serves as an obligatory subunit that is required both for supporting the basic activity and regulating the pH-sensing of NHE1 via interactions between distinct parts of these proteins.

	Crystal structure of CHP2 complexed with NHE1-cytosolic region and an implication for pH regulation.,Ammar YB, Takeda S, Hisamitsu T, Mori H, Wakabayashi S EMBO J. 2006 Jun 7;25(11):2315-25. Epub 2006 May 18. PMID:16710297<ref>PMID:16710297</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2bec" style="background-color:#fffaf0;"></div>~~
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Human~~]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Ammar, Y ~~Ben~~]]		[[Category: Ben Ammar Y]]
	[[Category: Hisamitsu, T]]		[[Category: Hisamitsu T]]
	[[Category: Mori, H]]		[[Category: Mori H]]
	[[Category: Takeda, S]]		[[Category: Takeda S]]
	[[Category: Wakabayashi, S]]		[[Category: Wakabayashi S]]
	~~[[Category: Calcineurin-homologous protein]]~~
	~~[[Category: Calcium-binding protein]]~~
	~~[[Category: Metal binding protein-transport protein complex]]~~
	~~[[Category: Nhe1 regulating protein~~]]

OCA at 13:08, 24 November 2021

2021-11-24T13:08:27Z

← Older revision		Revision as of 13:08, 24 November 2021
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	<StructureSection load='2bec' size='340' side='right'caption='[[2bec]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2bec' size='340' side='right'caption='[[2bec]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bec]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2BEC FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bec]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BEC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=YT3:YTTRIUM+(III)+ION'>YT3</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=YT3:YTTRIUM+(III)+ION'>YT3</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2bec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bec OCA], [~~http~~://pdbe.org/2bec PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2bec RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2bec PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2bec ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bec OCA], [https://pdbe.org/2bec PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bec RCSB], [https://www.ebi.ac.uk/pdbsum/2bec PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bec ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/CHP2_HUMAN CHP2_HUMAN]] Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and protecting cells from serum deprivation-induced death. Also plays a role in the regulation of cell proliferation and tumor growth by increasing the phosphatase activity of PPP3CA in a calcium-dependent manner. Activator of the calcineurin/NFAT signaling pathway. Involved in the cytoplasmic translocation of the transcription factor NFATC3 to the nucleus.<ref>PMID:12226101</ref> <ref>PMID:18815128</ref> [[~~http~~://www.uniprot.org/uniprot/SL9A1_HUMAN SL9A1_HUMAN]] Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.<ref>PMID:8901634</ref> <ref>PMID:11350981</ref> <ref>PMID:15035633</ref>		[[https://www.uniprot.org/uniprot/CHP2_HUMAN CHP2_HUMAN]] Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and protecting cells from serum deprivation-induced death. Also plays a role in the regulation of cell proliferation and tumor growth by increasing the phosphatase activity of PPP3CA in a calcium-dependent manner. Activator of the calcineurin/NFAT signaling pathway. Involved in the cytoplasmic translocation of the transcription factor NFATC3 to the nucleus.<ref>PMID:12226101</ref> <ref>PMID:18815128</ref> [[https://www.uniprot.org/uniprot/SL9A1_HUMAN SL9A1_HUMAN]] Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.<ref>PMID:8901634</ref> <ref>PMID:11350981</ref> <ref>PMID:15035633</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:01, 19 February 2020

2020-02-19T08:01:35Z

← Older revision		Revision as of 08:01, 19 February 2020
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	==Crystal structure of CHP2 in complex with its binding region in NHE1 and insights into the mechanism of pH regulation==		==Crystal structure of CHP2 in complex with its binding region in NHE1 and insights into the mechanism of pH regulation==
	<StructureSection load='2bec' size='340' side='right' caption='[[2bec]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2bec' size='340' side='right'caption='[[2bec]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bec]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEC FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bec]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEC FirstGlance]. <br>
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
			[[Category: Large Structures]]
	[[Category: Ammar, Y Ben]]		[[Category: Ammar, Y Ben]]
	[[Category: Hisamitsu, T]]		[[Category: Hisamitsu, T]]

OCA at 06:24, 16 May 2018

2018-05-16T06:24:28Z

← Older revision		Revision as of 06:24, 16 May 2018
Line 1:		Line 1:

	==Crystal structure of CHP2 in complex with its binding region in NHE1 and insights into the mechanism of pH regulation==		==Crystal structure of CHP2 in complex with its binding region in NHE1 and insights into the mechanism of pH regulation==
	<StructureSection load='2bec' size='340' side='right' caption='[[2bec]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2bec' size='340' side='right' caption='[[2bec]], [[Resolution\|resolution]] 2.70Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2bec]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEC FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bec]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=YT3:YTTRIUM+(III)+ION'>YT3</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=YT3:YTTRIUM+(III)+ION'>YT3</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bec OCA], [http://pdbe.org/2bec PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bec RCSB], [http://www.ebi.ac.uk/pdbsum/2bec PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bec OCA], [http://pdbe.org/2bec PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bec RCSB], [http://www.ebi.ac.uk/pdbsum/2bec PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2bec ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/be/2bec_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/be/2bec_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 11:02, 9 February 2016

2016-02-09T11:02:07Z

← Older revision		Revision as of 11:02, 9 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bec ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 21:39, 10 September 2015

2015-09-10T21:39:36Z

← Older revision		Revision as of 21:39, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2bec' size='340' side='right' caption='[[2bec]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2bec' size='340' side='right' caption='[[2bec]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bec]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEC FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bec]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=YT3:YTTRIUM+(III)+ION'>YT3</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=YT3:YTTRIUM+(III)+ION'>YT3</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bec OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bec RCSB], [http://www.ebi.ac.uk/pdbsum/2bec PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bec OCA], [http://pdbe.org/2bec PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bec RCSB], [http://www.ebi.ac.uk/pdbsum/2bec PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2bec" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Homo sapiens~~]]		[[Category: Human]]
	[[Category: Ammar, Y Ben]]		[[Category: Ammar, Y Ben]]
	[[Category: Hisamitsu, T]]		[[Category: Hisamitsu, T]]

OCA at 06:27, 25 December 2014

2014-12-25T06:27:06Z

← Older revision		Revision as of 06:27, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bec]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEC FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bec]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEC FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=YT3:YTTRIUM+(III)+ION'>YT3</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=YT3:YTTRIUM+(III)+ION'>YT3</scene></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bec OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bec RCSB], [http://www.ebi.ac.uk/pdbsum/2bec PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bec OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bec RCSB], [http://www.ebi.ac.uk/pdbsum/2bec PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/CHP2_HUMAN CHP2_HUMAN]] Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and protecting cells from serum deprivation-induced death. Also plays a role in the regulation of cell proliferation and tumor growth by increasing the phosphatase activity of PPP3CA in a calcium-dependent manner. Activator of the calcineurin/NFAT signaling pathway. Involved in the cytoplasmic translocation of the transcription factor NFATC3 to the nucleus.<ref>PMID:12226101</ref> <ref>PMID:18815128</ref> [[http://www.uniprot.org/uniprot/SL9A1_HUMAN SL9A1_HUMAN]] Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.<ref>PMID:8901634</ref> <ref>PMID:11350981</ref> <ref>PMID:15035633</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Ammar, Y Ben.]]		[[Category: Ammar, Y Ben]]
	[[Category: Hisamitsu, T.]]		[[Category: Hisamitsu, T]]
	[[Category: Mori, H.]]		[[Category: Mori, H]]
	[[Category: Takeda, S.]]		[[Category: Takeda, S]]
	[[Category: Wakabayashi, S.]]		[[Category: Wakabayashi, S]]
	[[Category: Calcineurin-homologous protein]]		[[Category: Calcineurin-homologous protein]]
	[[Category: Calcium-binding protein]]		[[Category: Calcium-binding protein]]
	[[Category: Metal binding protein-transport protein complex]]		[[Category: Metal binding protein-transport protein complex]]
	[[Category: Nhe1 regulating protein]]		[[Category: Nhe1 regulating protein]]

OCA at 02:33, 30 September 2014

2014-09-30T02:33:35Z

← Older revision		Revision as of 02:33, 30 September 2014
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	[[Image:~~2bec~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of CHP2 in complex with its binding region in NHE1 and insights into the mechanism of pH regulation==
			<StructureSection load='2bec' size='340' side='right' caption='[[2bec]], [[Resolution\|resolution]] 2.70Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2bec]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEC FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=YT3:YTTRIUM+(III)+ION'>YT3</scene><br>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bec OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bec RCSB], [http://www.ebi.ac.uk/pdbsum/2bec PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/be/2bec_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The plasma membrane Na+/H+ exchangers (NHE) require calcineurin B homologous protein (CHP) as an obligatory binding partner for ion transport. Here, we report the first crystal structure of CHP (CHP2 isoform) in complex with its binding domain in NHE1. We show that the cytoplasmic alpha-helix of NHE1 is inserted into the hydrophobic cleft formed by N- and C-lobes of CHP2 and that the size and shape of this crevice together with hydrogen bond formation at multiple positions assure a high degree of specificity for interaction with NHE members. Structure-based mutagenesis revealed the importance of hydrophobic interactions between CHP/NHE1 for the function of NHE1. Furthermore, the crystal structure shows the existence of a protruding CHP-unique region, and deletion of this region in CHP2 inhibited the NHE1 activity by inducing the acidic shift of intracellular pH dependence, while preserving interaction with NHE1. These findings suggest that CHP serves as an obligatory subunit that is required both for supporting the basic activity and regulating the pH-sensing of NHE1 via interactions between distinct parts of these proteins.

	~~{{STRUCTURE_2bec\| PDB=2bec \| SCENE= }}~~		Crystal structure of CHP2 complexed with NHE1-cytosolic region and an implication for pH regulation.,Ammar YB, Takeda S, Hisamitsu T, Mori H, Wakabayashi S EMBO J. 2006 Jun 7;25(11):2315-25. Epub 2006 May 18. PMID:16710297<ref>PMID:16710297</ref>

	~~===Crystal structure~~ of ~~CHP2 in complex with its binding region in NHE1 and insights into~~ the ~~mechanism of pH regulation===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_16710297}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2bec]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2BEC OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:016710297<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Ammar, Y Ben.]]		[[Category: Ammar, Y Ben.]]

OCA: Protected "2bec" [edit=sysop:move=sysop]

2013-01-06T11:45:57Z

Protected "2bec" [edit=sysop:move=sysop]

← Older revision	Revision as of 11:45, 6 January 2013
(No difference)

OCA at 11:45, 6 January 2013

2013-01-06T11:45:54Z

← Older revision		Revision as of 11:45, 6 January 2013
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	~~{{Seed}}~~
	[[Image:2bec.png\|left\|200px]]		[[Image:2bec.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2bec", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2bec\| PDB=2bec \| SCENE= }}		{{STRUCTURE_2bec\| PDB=2bec \| SCENE= }}

	===Crystal structure of CHP2 in complex with its binding region in NHE1 and insights into the mechanism of pH regulation===		===Crystal structure of CHP2 in complex with its binding region in NHE1 and insights into the mechanism of pH regulation===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_16710297}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 16710297 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_16710297}}		{{ABSTRACT_PUBMED_16710297}}

	==About this Structure==		==About this Structure==
	~~2BEC~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEC OCA].		[[2bec]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEC OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~16710297~~</ref><references group="xtra"/>		<ref group="xtra">PMID:016710297</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Ammar, Y Ben.]]		[[Category: Ammar, Y Ben.]]
Line 32:		Line 20:
	[[Category: Calcineurin-homologous protein]]		[[Category: Calcineurin-homologous protein]]
	[[Category: Calcium-binding protein]]		[[Category: Calcium-binding protein]]
			[[Category: Metal binding protein-transport protein complex]]
	[[Category: Nhe1 regulating protein]]		[[Category: Nhe1 regulating protein]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 09:20:56 2009''~~