2bem - Revision history

OCA at 09:01, 6 November 2024

2024-11-06T09:01:17Z

← Older revision		Revision as of 09:01, 6 November 2024
Line 9:		Line 9:
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/~~O83009_SERMA O83009_SERMA~~]		[https://www.uniprot.org/uniprot/LP10_SERMA LP10_SERMA] Lytic polysaccharide monooxygenase (LPMO) that oxidatively cleaves alpha- and beta-chitin with C1 regioselectivity (PubMed:23112164). Catalysis by LPMOs requires the reduction of the active-site copper from Cu(II) to Cu(I) by a reducing agent and H(2)O(2) or O(2) as a cosubstrate (PubMed:23112164).<ref>PMID:23112164</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 15:		Line 15:
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/be/2bem_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/be/2bem_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/~~initialview01~~.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>

OCA at 11:21, 22 May 2024

2024-05-22T11:21:23Z

← Older revision		Revision as of 11:21, 22 May 2024
Line 3:		Line 3:
	<StructureSection load='2bem' size='340' side='right'caption='[[2bem]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='2bem' size='340' side='right'caption='[[2bem]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bem]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_marcescens"_(bizio_1823)_trevisan_in_de_toni_and_trevisan_1889 "bacillus~~ marcescens~~" (bizio 1823) trevisan in de toni and trevisan 1889~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BEM FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bem]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BEM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.55Å</td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ben\|2ben]]</div~~></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bem OCA], [https://pdbe.org/2bem PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bem RCSB], [https://www.ebi.ac.uk/pdbsum/2bem PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bem ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bem OCA], [https://pdbe.org/2bem PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bem RCSB], [https://www.ebi.ac.uk/pdbsum/2bem PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bem ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/O83009_SERMA O83009_SERMA]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Aalten, D M.F van~~]]		[[Category: Serratia marcescens]]
	[[Category: Eijsink~~, V G.H~~]]		[[Category: Eijsink VGH]]
	[[Category: Houston~~, D R~~]]		[[Category: Houston DR]]
	[[Category: Vaaje-Kolstad, G]]		[[Category: Vaaje-Kolstad G]]
	[[Category: ~~Chitin degradation]]~~		[[Category: Van Aalten DMF]]
	~~[[Category: Chitin-binding]]~~
	~~[[Category: Chitin-binding protein]]~~
	~~[[Category: Fniii-like fold~~]]

OCA at 13:08, 24 November 2021

2021-11-24T13:08:36Z

← Older revision		Revision as of 13:08, 24 November 2021
Line 3:		Line 3:
	<StructureSection load='2bem' size='340' side='right'caption='[[2bem]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='2bem' size='340' side='right'caption='[[2bem]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bem]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_marcescens"_(bizio_1823)_trevisan_in_de_toni_and_trevisan_1889 "bacillus marcescens" (bizio 1823) trevisan in de toni and trevisan 1889]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2BEM FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bem]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_marcescens"_(bizio_1823)_trevisan_in_de_toni_and_trevisan_1889 "bacillus marcescens" (bizio 1823) trevisan in de toni and trevisan 1889]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BEM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ben\|2ben]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ben\|2ben]]</div></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2bem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bem OCA], [~~http~~://pdbe.org/2bem PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2bem RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2bem PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2bem ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bem OCA], [https://pdbe.org/2bem PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bem RCSB], [https://www.ebi.ac.uk/pdbsum/2bem PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bem ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 08:17, 19 February 2020

2020-02-19T08:17:13Z

← Older revision		Revision as of 08:17, 19 February 2020
Line 1:		Line 1:

	==Crystal structure of the Serratia marcescens chitin-binding protein CBP21==		==Crystal structure of the Serratia marcescens chitin-binding protein CBP21==
	<StructureSection load='2bem' size='340' side='right' caption='[[2bem]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='2bem' size='340' side='right'caption='[[2bem]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bem]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_marcescens"_(bizio_1823)_trevisan_in_de_toni_and_trevisan_1889 "bacillus marcescens" (bizio 1823) trevisan in de toni and trevisan 1889]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEM FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bem]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_marcescens"_(bizio_1823)_trevisan_in_de_toni_and_trevisan_1889 "bacillus marcescens" (bizio 1823) trevisan in de toni and trevisan 1889]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEM FirstGlance]. <br>
Line 31:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Aalten, D M.F van]]		[[Category: Aalten, D M.F van]]
	[[Category: Eijsink, V G.H]]		[[Category: Eijsink, V G.H]]

OCA at 06:48, 16 May 2018

2018-05-16T06:48:38Z

← Older revision		Revision as of 06:48, 16 May 2018
Line 1:		Line 1:
	==~~CRYSTAL STRUCTURE OF THE SERRATIA MARCESCENS CHITIN~~-~~BINDING PROTEIN~~ CBP21==
			==Crystal structure of the Serratia marcescens chitin-binding protein CBP21==
	<StructureSection load='2bem' size='340' side='right' caption='[[2bem]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='2bem' size='340' side='right' caption='[[2bem]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ben\|2ben]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ben\|2ben]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bem OCA], [http://pdbe.org/2bem PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bem RCSB], [http://www.ebi.ac.uk/pdbsum/2bem PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bem OCA], [http://pdbe.org/2bem PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bem RCSB], [http://www.ebi.ac.uk/pdbsum/2bem PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2bem ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/be/2bem_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/be/2bem_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 30:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Aalten, D M.F ~~Van~~]]		[[Category: Aalten, D M.F van]]
	[[Category: Eijsink, V G.H]]		[[Category: Eijsink, V G.H]]
	[[Category: Houston, D R]]		[[Category: Houston, D R]]

OCA at 17:34, 7 February 2016

2016-02-07T17:34:34Z

← Older revision		Revision as of 17:34, 7 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bem ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 03:40, 10 September 2015

2015-09-10T03:40:58Z

← Older revision		Revision as of 03:40, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2bem' size='340' side='right' caption='[[2bem]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='2bem' size='340' side='right' caption='[[2bem]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bem]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Serratia_marcescens Serratia~~ marcescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEM FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bem]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_marcescens"_(bizio_1823)_trevisan_in_de_toni_and_trevisan_1889 "bacillus marcescens" (bizio 1823) trevisan in de toni and trevisan 1889]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ben\|2ben]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ben\|2ben]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bem OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bem RCSB], [http://www.ebi.ac.uk/pdbsum/2bem PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bem OCA], [http://pdbe.org/2bem PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bem RCSB], [http://www.ebi.ac.uk/pdbsum/2bem PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2bem" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Serratia marcescens]]~~
	[[Category: Aalten, D M.F Van]]		[[Category: Aalten, D M.F Van]]
	[[Category: Eijsink, V G.H]]		[[Category: Eijsink, V G.H]]

OCA at 10:36, 8 January 2015

2015-01-08T10:36:57Z

← Older revision		Revision as of 10:36, 8 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bem]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEM FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bem]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEM FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ben\|2ben]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ben\|2ben]]</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bem OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bem RCSB], [http://www.ebi.ac.uk/pdbsum/2bem PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bem OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bem RCSB], [http://www.ebi.ac.uk/pdbsum/2bem PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Serratia marcescens]]		[[Category: Serratia marcescens]]
	[[Category: Aalten, D M.F Van.]]		[[Category: Aalten, D M.F Van]]
	[[Category: Eijsink, V G.H.]]		[[Category: Eijsink, V G.H]]
	[[Category: Houston, D R.]]		[[Category: Houston, D R]]
	[[Category: Vaaje-Kolstad, G.]]		[[Category: Vaaje-Kolstad, G]]
	[[Category: Chitin degradation]]		[[Category: Chitin degradation]]
	[[Category: Chitin-binding]]		[[Category: Chitin-binding]]
	[[Category: Chitin-binding protein]]		[[Category: Chitin-binding protein]]
	[[Category: Fniii-like fold]]		[[Category: Fniii-like fold]]

OCA at 03:00, 30 September 2014

2014-09-30T03:00:21Z

← Older revision		Revision as of 03:00, 30 September 2014
Line 1:		Line 1:
	[[Image:~~2bem~~.~~png~~\|~~left~~\|~~200px~~]]		==CRYSTAL STRUCTURE OF THE SERRATIA MARCESCENS CHITIN-BINDING PROTEIN CBP21==
			<StructureSection load='2bem' size='340' side='right' caption='[[2bem]], [[Resolution\|resolution]] 1.55Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2bem]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BEM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BEM FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ben\|2ben]]</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bem FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bem OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bem RCSB], [http://www.ebi.ac.uk/pdbsum/2bem PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/be/2bem_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Chitin proteins are commonly found in bacteria that utilize chitin as a source of energy. CBP21 is a chitin-binding protein from Serratia marcescens, a Gram-negative soil bacterium capable of efficient chitin degradation. When grown on chitin, S. marcescens secretes large amounts of CBP21, along with chitin-degrading enzymes. In an attempt to understand the molecular mechanism of CBP21 action, we have determined its crystal structure at 1.55 angstroms resolution. This is the first structure to be solved of a family 33 carbohydrate-binding module. The structure reveals a "budded" fibronectin type III fold consisting of two beta-sheets, arranged as a beta-sheet sandwich, with a 65-residue "bud" consisting of three short helices, located between beta-strands 1 and 2. Remarkably, conserved aromatic residues that have been suggested previously to play a role in chitin binding were mainly found in the interior of the protein, seemingly incapable of interacting with chitin, whereas the structure revealed a surface patch of highly conserved, mainly hydrophilic residues. The roles of six of these conserved surface-exposed residues (Tyr-54, Glu-55, Glu-60, His-114, Asp-182, and Asn-185) were probed by site-directed mutagenesis and subsequent binding studies. All single point mutations lowered the affinity of CBP21 for beta-chitin, as shown by 3-8-fold increases in the apparent binding constant. Thus, binding of CBP21 to chitin seems to be mediated primarily by conserved, solvent-exposed, polar side chains.

	~~{{STRUCTURE_2bem\| PDB=2bem \| SCENE= }}~~		Crystal structure and binding properties of the Serratia marcescens chitin-binding protein CBP21.,Vaaje-Kolstad G, Houston DR, Riemen AH, Eijsink VG, van Aalten DM J Biol Chem. 2005 Mar 25;280(12):11313-9. Epub 2004 Dec 8. PMID:15590674<ref>PMID:15590674</ref>

	~~===CRYSTAL STRUCTURE OF THE SERRATIA MARCESCENS CHITIN-BINDING PROTEIN CBP21===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_15590674}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2bem]] is~~ a ~~3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Serratia_marcescens Serratia marcescens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2BEM OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:015590674<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Serratia marcescens]]		[[Category: Serratia marcescens]]
	[[Category: Aalten, D M.F Van.]]		[[Category: Aalten, D M.F Van.]]

OCA: Protected "2bem" [edit=sysop:move=sysop]

2013-01-06T12:10:13Z

Protected "2bem" [edit=sysop:move=sysop]

← Older revision	Revision as of 12:10, 6 January 2013
(No difference)