2bf3 - Revision history

OCA at 06:37, 1 May 2024

2024-05-01T06:37:54Z

← Older revision		Revision as of 06:37, 1 May 2024
Line 3:		Line 3:
	<StructureSection load='2bf3' size='340' side='right'caption='[[2bf3]], [[Resolution\|resolution]] 1.96Å' scene=''>		<StructureSection load='2bf3' size='340' side='right'caption='[[2bf3]], [[Resolution\|resolution]] 1.96Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bf3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_25411 Atcc 25411~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BF3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BF3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bf3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_mendocina Pseudomonas mendocina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BF3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BF3 FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=HTO:HEPTANE~~-1~~,2,3-TRIOL'>HTO</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.96Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[1g10\|1g10]], [[1g11\|1g11]], [[2bf2\|2bf2]], [[2bf5\|2bf5]]~~</~~div~~></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bf3 OCA], [https://pdbe.org/2bf3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bf3 RCSB], [https://www.ebi.ac.uk/pdbsum/2bf3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bf3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bf3 OCA], [https://pdbe.org/2bf3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bf3 RCSB], [https://www.ebi.ac.uk/pdbsum/2bf3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bf3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/TMOD_PSEME TMOD_PSEME]] Effector protein subunit of the multicomponent enzyme toluene-4-monooxygenase that hydroxylates toluene to form p-cresol. Required for optimal efficiency and specificity of the holoenzyme.<ref>PMID:11297417</ref> <ref>PMID:15882052</ref> <ref>PMID:19033467</ref>		[https://www.uniprot.org/uniprot/TMOD_PSEME TMOD_PSEME] Effector protein subunit of the multicomponent enzyme toluene-4-monooxygenase that hydroxylates toluene to form p-cresol. Required for optimal efficiency and specificity of the holoenzyme.<ref>PMID:11297417</ref> <ref>PMID:15882052</ref> <ref>PMID:19033467</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bf3 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bf3 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Toluene 4-monooxygenase (T4MO) is a four-component complex that catalyzes the regiospecific, NADH-dependent hydroxylation of toluene to yield p-cresol. The catalytic effector (T4moD) of this complex is a 102-residue protein devoid of metals or organic cofactors. It forms a complex with the diiron hydroxylase component (T4moH) that influences both the kinetics and regiospecificity of catalysis. Here, we report crystal structures for native T4moD and two engineered variants with either four (DeltaN4-) or 10 (DeltaN10-) residues removed from the N-terminal at 2.1-, 1.7-, and 1.9-A resolution, respectively. The crystal structures have C-alpha root-mean-squared differences of less than 0.8 A for the central core consisting of residues 11-98, showing that alterations of the N-terminal have little influence on the folded core of the protein. The central core has the same fold topology as observed in the NMR structures of T4moD, the methane monooxygenase effector protein (MmoB) from two methanotrophs, and the phenol hydroxylase effector protein (DmpM). However, the root-mean-squared differences between comparable C-alpha positions in the X-ray structures and the NMR structures vary from approximately 1.8 A to greater than 6 A. The X-ray structures exhibit an estimated overall coordinate error from 0.095 (0.094) A based on the R-value (R free) for the highest resolution DeltaN4-T4moD structure to 0.211 (0.196) A for the native T4moD structure. Catalytic studies of the DeltaN4-, DeltaN7-, and DeltaN10- variants of T4moD show statistically insignificant changes in k(cat), K(M), k(cat)/K(M), and K(I) relative to the native protein. Moreover, there was no significant change in the regiospecificity of toluene oxidation with any of the T4moD variants. The relative insensitivity to changes in the N-terminal region distinguishes T4moD from the MmoB homologues, which each require the approximately 33 residue N-terminal region for catalytic activity.

	Crystal structures and functional studies of T4moD, the toluene 4-monooxygenase catalytic effector protein.,Lountos GT, Mitchell KH, Studts JM, Fox BG, Orville AM Biochemistry. 2005 May 17;44(19):7131-42. PMID:15882052<ref>PMID:15882052</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2bf3" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
Line 36:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Atcc 25411]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Fox, B G~~]]		[[Category: Pseudomonas mendocina]]
	[[Category: ~~Lountos, G T~~]]		[[Category: Fox BG]]
	[[Category: ~~Mitchell, K H~~]]		[[Category: Lountos GT]]
	[[Category: ~~Orville, A M~~]]		[[Category: Mitchell KH]]
	[[Category: ~~Studts, J M~~]]		[[Category: Orville AM]]
	[[Category: ~~Aromatic hydrocarbon catabolism]]~~		[[Category: Studts JM]]
	~~[[Category: Catalytic effector protein]]~~
	~~[[Category: Molecular replacement]]~~
	~~[[Category: Monooxygenase]]~~
	~~[[Category: N-terminal truncated mutant]]~~
	~~[[Category: Oxidoreductase]]~~
	~~[[Category: Toluene oxidation~~]]

OCA at 13:08, 24 November 2021

2021-11-24T13:08:53Z

← Older revision		Revision as of 13:08, 24 November 2021
Line 3:		Line 3:
	<StructureSection load='2bf3' size='340' side='right'caption='[[2bf3]], [[Resolution\|resolution]] 1.96Å' scene=''>		<StructureSection load='2bf3' size='340' side='right'caption='[[2bf3]], [[Resolution\|resolution]] 1.96Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bf3]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_25411 Atcc 25411]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BF3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2BF3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bf3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_25411 Atcc 25411]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BF3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BF3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g10\|1g10]], [[1g11\|1g11]], [[2bf2\|2bf2]], [[2bf5\|2bf5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1g10\|1g10]], [[1g11\|1g11]], [[2bf2\|2bf2]], [[2bf5\|2bf5]]</div></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2bf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bf3 OCA], [~~http~~://pdbe.org/2bf3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2bf3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2bf3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2bf3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bf3 OCA], [https://pdbe.org/2bf3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bf3 RCSB], [https://www.ebi.ac.uk/pdbsum/2bf3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bf3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/TMOD_PSEME TMOD_PSEME]] Effector protein subunit of the multicomponent enzyme toluene-4-monooxygenase that hydroxylates toluene to form p-cresol. Required for optimal efficiency and specificity of the holoenzyme.<ref>PMID:11297417</ref> <ref>PMID:15882052</ref> <ref>PMID:19033467</ref>		[[https://www.uniprot.org/uniprot/TMOD_PSEME TMOD_PSEME]] Effector protein subunit of the multicomponent enzyme toluene-4-monooxygenase that hydroxylates toluene to form p-cresol. Required for optimal efficiency and specificity of the holoenzyme.<ref>PMID:11297417</ref> <ref>PMID:15882052</ref> <ref>PMID:19033467</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:55, 19 February 2020

2020-02-19T07:55:34Z

← Older revision		Revision as of 07:55, 19 February 2020
Line 1:		Line 1:

	==Crystal structure of a toluene 4-monooxygenase catalytic effector protein variant missing ten N-terminal residues (delta-N10 T4moD)==		==Crystal structure of a toluene 4-monooxygenase catalytic effector protein variant missing ten N-terminal residues (delta-N10 T4moD)==
	<StructureSection load='2bf3' size='340' side='right' caption='[[2bf3]], [[Resolution\|resolution]] 1.96Å' scene=''>		<StructureSection load='2bf3' size='340' side='right'caption='[[2bf3]], [[Resolution\|resolution]] 1.96Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bf3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_25411 Atcc 25411]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BF3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BF3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bf3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_25411 Atcc 25411]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BF3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BF3 FirstGlance]. <br>
Line 31:		Line 31:

	==See Also==		==See Also==
	*[[Monooxygenase\|Monooxygenase]]		*[[Monooxygenase 3D structures\|Monooxygenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 37:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 25411]]		[[Category: Atcc 25411]]
			[[Category: Large Structures]]
	[[Category: Fox, B G]]		[[Category: Fox, B G]]
	[[Category: Lountos, G T]]		[[Category: Lountos, G T]]

OCA at 06:14, 16 May 2018

2018-05-16T06:14:27Z

← Older revision		Revision as of 06:14, 16 May 2018
Line 1:		Line 1:
	==~~CRYSTAL STRUCTURE OF A TOLUENE~~ 4-~~MONOOXYGENASE CATALYTIC EFFECTOR PROTEIN VARIANT MISSING TEN~~ N-~~TERMINAL RESIDUES~~ (~~DELTA~~-N10 ~~T4MOD~~)==
			==Crystal structure of a toluene 4-monooxygenase catalytic effector protein variant missing ten N-terminal residues (delta-N10 T4moD)==
	<StructureSection load='2bf3' size='340' side='right' caption='[[2bf3]], [[Resolution\|resolution]] 1.96Å' scene=''>		<StructureSection load='2bf3' size='340' side='right' caption='[[2bf3]], [[Resolution\|resolution]] 1.96Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g10\|1g10]], [[1g11\|1g11]], [[2bf2\|2bf2]], [[2bf5\|2bf5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g10\|1g10]], [[1g11\|1g11]], [[2bf2\|2bf2]], [[2bf5\|2bf5]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bf3 OCA], [http://pdbe.org/2bf3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bf3 RCSB], [http://www.ebi.ac.uk/pdbsum/2bf3 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bf3 OCA], [http://pdbe.org/2bf3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bf3 RCSB], [http://www.ebi.ac.uk/pdbsum/2bf3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2bf3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bf/2bf3_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bf/2bf3_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 2bf3" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2bf3" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Monooxygenase\|Monooxygenase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 23:17, 9 February 2016

2016-02-09T23:17:49Z

← Older revision		Revision as of 23:17, 9 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bf3 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 03:01, 11 September 2015

2015-09-11T03:01:39Z

← Older revision		Revision as of 03:01, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2bf3' size='340' side='right' caption='[[2bf3]], [[Resolution\|resolution]] 1.96Å' scene=''>		<StructureSection load='2bf3' size='340' side='right' caption='[[2bf3]], [[Resolution\|resolution]] 1.96Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bf3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_mendocina Pseudomonas mendocina~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BF3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BF3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bf3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_25411 Atcc 25411]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BF3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BF3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g10\|1g10]], [[1g11\|1g11]], [[2bf2\|2bf2]], [[2bf5\|2bf5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g10\|1g10]], [[1g11\|1g11]], [[2bf2\|2bf2]], [[2bf5\|2bf5]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bf3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bf3 RCSB], [http://www.ebi.ac.uk/pdbsum/2bf3 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bf3 OCA], [http://pdbe.org/2bf3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bf3 RCSB], [http://www.ebi.ac.uk/pdbsum/2bf3 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2bf3" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Pseudomonas mendocina~~]]		[[Category: Atcc 25411]]
	[[Category: Fox, B G]]		[[Category: Fox, B G]]
	[[Category: Lountos, G T]]		[[Category: Lountos, G T]]

OCA at 10:11, 25 December 2014

2014-12-25T10:11:01Z

← Older revision		Revision as of 10:11, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bf3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_mendocina Pseudomonas mendocina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BF3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BF3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bf3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_mendocina Pseudomonas mendocina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BF3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BF3 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g10\|1g10]], [[1g11\|1g11]], [[2bf2\|2bf2]], [[2bf5\|2bf5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g10\|1g10]], [[1g11\|1g11]], [[2bf2\|2bf2]], [[2bf5\|2bf5]]</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bf3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bf3 RCSB], [http://www.ebi.ac.uk/pdbsum/2bf3 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bf3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bf3 RCSB], [http://www.ebi.ac.uk/pdbsum/2bf3 PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/TMOD_PSEME TMOD_PSEME]] Effector protein subunit of the multicomponent enzyme toluene-4-monooxygenase that hydroxylates toluene to form p-cresol. Required for optimal efficiency and specificity of the holoenzyme.<ref>PMID:11297417</ref> <ref>PMID:15882052</ref> <ref>PMID:19033467</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Pseudomonas mendocina]]		[[Category: Pseudomonas mendocina]]
	[[Category: Fox, B G.]]		[[Category: Fox, B G]]
	[[Category: Lountos, G T.]]		[[Category: Lountos, G T]]
	[[Category: Mitchell, K H.]]		[[Category: Mitchell, K H]]
	[[Category: Orville, A M.]]		[[Category: Orville, A M]]
	[[Category: Studts, J M.]]		[[Category: Studts, J M]]
	[[Category: Aromatic hydrocarbon catabolism]]		[[Category: Aromatic hydrocarbon catabolism]]
	[[Category: Catalytic effector protein]]		[[Category: Catalytic effector protein]]

OCA at 01:35, 30 September 2014

2014-09-30T01:35:01Z

← Older revision		Revision as of 01:35, 30 September 2014
Line 1:		Line 1:
	[[Image:~~2bf3~~.~~png~~\|~~left~~\|~~200px~~]]		==CRYSTAL STRUCTURE OF A TOLUENE 4-MONOOXYGENASE CATALYTIC EFFECTOR PROTEIN VARIANT MISSING TEN N-TERMINAL RESIDUES (DELTA-N10 T4MOD)==
			<StructureSection load='2bf3' size='340' side='right' caption='[[2bf3]], [[Resolution\|resolution]] 1.96Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2bf3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_mendocina Pseudomonas mendocina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BF3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BF3 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1g10\|1g10]], [[1g11\|1g11]], [[2bf2\|2bf2]], [[2bf5\|2bf5]]</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bf3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bf3 RCSB], [http://www.ebi.ac.uk/pdbsum/2bf3 PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bf/2bf3_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Toluene 4-monooxygenase (T4MO) is a four-component complex that catalyzes the regiospecific, NADH-dependent hydroxylation of toluene to yield p-cresol. The catalytic effector (T4moD) of this complex is a 102-residue protein devoid of metals or organic cofactors. It forms a complex with the diiron hydroxylase component (T4moH) that influences both the kinetics and regiospecificity of catalysis. Here, we report crystal structures for native T4moD and two engineered variants with either four (DeltaN4-) or 10 (DeltaN10-) residues removed from the N-terminal at 2.1-, 1.7-, and 1.9-A resolution, respectively. The crystal structures have C-alpha root-mean-squared differences of less than 0.8 A for the central core consisting of residues 11-98, showing that alterations of the N-terminal have little influence on the folded core of the protein. The central core has the same fold topology as observed in the NMR structures of T4moD, the methane monooxygenase effector protein (MmoB) from two methanotrophs, and the phenol hydroxylase effector protein (DmpM). However, the root-mean-squared differences between comparable C-alpha positions in the X-ray structures and the NMR structures vary from approximately 1.8 A to greater than 6 A. The X-ray structures exhibit an estimated overall coordinate error from 0.095 (0.094) A based on the R-value (R free) for the highest resolution DeltaN4-T4moD structure to 0.211 (0.196) A for the native T4moD structure. Catalytic studies of the DeltaN4-, DeltaN7-, and DeltaN10- variants of T4moD show statistically insignificant changes in k(cat), K(M), k(cat)/K(M), and K(I) relative to the native protein. Moreover, there was no significant change in the regiospecificity of toluene oxidation with any of the T4moD variants. The relative insensitivity to changes in the N-terminal region distinguishes T4moD from the MmoB homologues, which each require the approximately 33 residue N-terminal region for catalytic activity.

	~~{{STRUCTURE_2bf3\| PDB=2bf3 \| SCENE= }}~~		Crystal structures and functional studies of T4moD, the toluene 4-monooxygenase catalytic effector protein.,Lountos GT, Mitchell KH, Studts JM, Fox BG, Orville AM Biochemistry. 2005 May 17;44(19):7131-42. PMID:15882052<ref>PMID:15882052</ref>

	~~===CRYSTAL STRUCTURE OF A TOLUENE 4-MONOOXYGENASE CATALYTIC EFFECTOR PROTEIN VARIANT MISSING TEN N-TERMINAL RESIDUES (DELTA-N10 T4MOD)===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_15882052}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2bf3]] is~~ a ~~2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_mendocina Pseudomonas mendocina]. Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il/oca-bin/ocashort?id=2BF3 OCA]~~.		</StructureSection>

	~~==Reference==~~
	<~~ref group="xtra"~~>~~PMID:015882052~~</~~ref~~>~~<ref group~~=~~"xtra">PMID:012595730</ref><ref group~~=~~"xtra">PMID:010336868</ref><ref group~~=~~"xtra">PMID:008703915~~</~~ref~~><~~references group="xtra"~~/>
	[[Category: Pseudomonas mendocina]]		[[Category: Pseudomonas mendocina]]
	[[Category: Fox, B G.]]		[[Category: Fox, B G.]]

OCA at 12:16, 23 January 2013

2013-01-23T12:16:38Z

← Older revision		Revision as of 12:16, 23 January 2013
Line 1:		Line 1:
	[[Image:2bf3.png\|left\|200px]]		[[Image:2bf3.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2bf3", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2bf3\| PDB=2bf3 \| SCENE= }}		{{STRUCTURE_2bf3\| PDB=2bf3 \| SCENE= }}

	===CRYSTAL STRUCTURE OF A TOLUENE 4-MONOOXYGENASE CATALYTIC EFFECTOR PROTEIN VARIANT MISSING TEN N-TERMINAL RESIDUES (DELTA-N10 T4MOD)===		===CRYSTAL STRUCTURE OF A TOLUENE 4-MONOOXYGENASE CATALYTIC EFFECTOR PROTEIN VARIANT MISSING TEN N-TERMINAL RESIDUES (DELTA-N10 T4MOD)===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_15882052}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 15882052 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_15882052}}		{{ABSTRACT_PUBMED_15882052}}

Line 22:		Line 11:

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~15882052~~</ref><ref group="xtra">PMID:~~12595730~~</ref><ref group="xtra">PMID:~~11297417~~</ref><ref group="xtra">PMID:~~10336868</ref><ref group="xtra">PMID:11863457</ref><ref group="xtra">PMID:8703915~~</ref><references group="xtra"/>		<ref group="xtra">PMID:015882052</ref><ref group="xtra">PMID:012595730</ref><ref group="xtra">PMID:010336868</ref><ref group="xtra">PMID:008703915</ref><references group="xtra"/>
	[[Category: Pseudomonas mendocina]]		[[Category: Pseudomonas mendocina]]
	[[Category: Fox, B G.]]		[[Category: Fox, B G.]]

OCA: Protected "2bf3" [edit=sysop:move=sysop]

2011-03-14T21:27:57Z

Protected "2bf3" [edit=sysop:move=sysop]

← Older revision	Revision as of 21:27, 14 March 2011
(No difference)