2bfa - Revision history

OCA at 13:31, 13 December 2023

2023-12-13T13:31:59Z

← Older revision		Revision as of 13:31, 13 December 2023
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	<StructureSection load='2bfa' size='340' side='right'caption='[[2bfa]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2bfa' size='340' side='right'caption='[[2bfa]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bfa]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Leima Leima~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BFA FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bfa]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BFA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CB3:10-PROPARGYL-5,8-DIDEAZAFOLIC+ACID'>CB3</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene~~></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1e7w\|1e7w]], [[1e92\|1e92]], [[1w0c\|1w0c]], [[2bf7\|2bf7]], [[2bfm\|2bfm]], [[2bfo\|2bfo]], [[2bfp\|2bfp]]</div></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CB3:10-PROPARGYL-5,8-DIDEAZAFOLIC+ACID'>CB3</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Pteridine_reductase Pteridine reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.33 1.5.1.33] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfa OCA], [https://pdbe.org/2bfa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bfa RCSB], [https://www.ebi.ac.uk/pdbsum/2bfa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bfa ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfa OCA], [https://pdbe.org/2bfa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bfa RCSB], [https://www.ebi.ac.uk/pdbsum/2bfa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bfa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/PTR1_LEIMA PTR1_LEIMA]] Exhibits a NADPH-dependent biopterin reductase activity. Has good activity with folate and significant activity with dihydrofolate and dihydrobiopterin, but not with quinonoid dihydrobiopterin. Confers resistance to methotrexate (MTX).<ref>PMID:7972081</ref>		[https://www.uniprot.org/uniprot/PTR1_LEIMA PTR1_LEIMA] Exhibits a NADPH-dependent biopterin reductase activity. Has good activity with folate and significant activity with dihydrofolate and dihydrobiopterin, but not with quinonoid dihydrobiopterin. Confers resistance to methotrexate (MTX).<ref>PMID:7972081</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Leima]]~~		[[Category: Leishmania major]]
	~~[[Category: Pteridine reductase~~]]		[[Category: Hunter WN]]
	[[Category: Hunter~~, W N~~]]		[[Category: Schuettelkopf AW]]
	[[Category: Schuettelkopf~~, A W]]~~
	~~[[Category: Drug resistance]]~~
	~~[[Category: Methotrexate resistance]]~~
	~~[[Category: Nadp]]~~
	~~[[Category: Oxidoreductase]]~~
	~~[[Category: Pterin salvage]]~~
	~~[[Category: Short-chain dehydrogenase/reductase]]~~
	~~[[Category: Trypanosomatid~~]]

OCA at 11:41, 3 February 2021

2021-02-03T11:41:13Z

← Older revision		Revision as of 11:41, 3 February 2021
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	==Leishmania major pteridine reductase 1 in complex with NADP and CB3717==		==Leishmania major pteridine reductase 1 in complex with NADP and CB3717==
	<StructureSection load='2bfa' size='340' side='right' caption='[[2bfa]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2bfa' size='340' side='right'caption='[[2bfa]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bfa]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Leima Leima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2BFA FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bfa]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Leima Leima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BFA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CB3:10-PROPARGYL-5,8-DIDEAZAFOLIC+ACID'>CB3</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CB3:10-PROPARGYL-5,8-DIDEAZAFOLIC+ACID'>CB3</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e7w\|1e7w]], [[1e92\|1e92]], [[1w0c\|1w0c]], [[2bf7\|2bf7]], [[2bfm\|2bfm]], [[2bfo\|2bfo]], [[2bfp\|2bfp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1e7w\|1e7w]], [[1e92\|1e92]], [[1w0c\|1w0c]], [[2bf7\|2bf7]], [[2bfm\|2bfm]], [[2bfo\|2bfo]], [[2bfp\|2bfp]]</div></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Pteridine_reductase Pteridine reductase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.33 1.5.1.33] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Pteridine_reductase Pteridine reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.33 1.5.1.33] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2bfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfa OCA], [~~http~~://pdbe.org/2bfa PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2bfa RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2bfa PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2bfa ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfa OCA], [https://pdbe.org/2bfa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bfa RCSB], [https://www.ebi.ac.uk/pdbsum/2bfa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bfa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/PTR1_LEIMA PTR1_LEIMA]] Exhibits a NADPH-dependent biopterin reductase activity. Has good activity with folate and significant activity with dihydrofolate and dihydrobiopterin, but not with quinonoid dihydrobiopterin. Confers resistance to methotrexate (MTX).<ref>PMID:7972081</ref>		[[https://www.uniprot.org/uniprot/PTR1_LEIMA PTR1_LEIMA]] Exhibits a NADPH-dependent biopterin reductase activity. Has good activity with folate and significant activity with dihydrofolate and dihydrobiopterin, but not with quinonoid dihydrobiopterin. Confers resistance to methotrexate (MTX).<ref>PMID:7972081</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Leima]]		[[Category: Leima]]
	[[Category: Pteridine reductase]]		[[Category: Pteridine reductase]]

OCA at 07:44, 6 March 2019

2019-03-06T07:44:52Z

← Older revision		Revision as of 07:44, 6 March 2019
Line 1:		Line 1:

	==Leishmania major pteridine reductase 1 in complex with NADP and CB3717==		==Leishmania major pteridine reductase 1 in complex with NADP and CB3717==
	<StructureSection load='2bfa' size='340' side='right' caption='[[2bfa]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2bfa' size='340' side='right' caption='[[2bfa]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bfa]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Leima Leima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BFA FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bfa]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Leima Leima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BFA FirstGlance]. <br>

OCA at 06:15, 16 May 2018

2018-05-16T06:15:00Z

← Older revision		Revision as of 06:15, 16 May 2018
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	==~~LEISHMANIA MAJOR PTERIDINE REDUCTASE~~ 1 ~~IN COMPLEX WITH~~ NADP ~~AND~~ CB3717==
			==Leishmania major pteridine reductase 1 in complex with NADP and CB3717==
	<StructureSection load='2bfa' size='340' side='right' caption='[[2bfa]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2bfa' size='340' side='right' caption='[[2bfa]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e7w\|1e7w]], [[1e92\|1e92]], [[1w0c\|1w0c]], [[2bf7\|2bf7]], [[2bfm\|2bfm]], [[2bfo\|2bfo]], [[2bfp\|2bfp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e7w\|1e7w]], [[1e92\|1e92]], [[1w0c\|1w0c]], [[2bf7\|2bf7]], [[2bfm\|2bfm]], [[2bfo\|2bfo]], [[2bfp\|2bfp]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pteridine_reductase Pteridine reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.33 1.5.1.33] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pteridine_reductase Pteridine reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.33 1.5.1.33] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfa OCA], [http://pdbe.org/2bfa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bfa RCSB], [http://www.ebi.ac.uk/pdbsum/2bfa PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfa OCA], [http://pdbe.org/2bfa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bfa RCSB], [http://www.ebi.ac.uk/pdbsum/2bfa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2bfa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bf/2bfa_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bf/2bfa_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 12:19, 7 February 2016

2016-02-07T12:19:40Z

← Older revision		Revision as of 12:19, 7 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bfa ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 01:27, 10 September 2015

2015-09-10T01:27:10Z

← Older revision		Revision as of 01:27, 10 September 2015
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	<StructureSection load='2bfa' size='340' side='right' caption='[[2bfa]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2bfa' size='340' side='right' caption='[[2bfa]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bfa]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Leishmania_major Leishmania major~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BFA FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bfa]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Leima Leima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BFA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CB3:10-PROPARGYL-5,8-DIDEAZAFOLIC+ACID'>CB3</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CB3:10-PROPARGYL-5,8-DIDEAZAFOLIC+ACID'>CB3</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e7w\|1e7w]], [[1e92\|1e92]], [[1w0c\|1w0c]], [[2bf7\|2bf7]], [[2bfm\|2bfm]], [[2bfo\|2bfo]], [[2bfp\|2bfp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e7w\|1e7w]], [[1e92\|1e92]], [[1w0c\|1w0c]], [[2bf7\|2bf7]], [[2bfm\|2bfm]], [[2bfo\|2bfo]], [[2bfp\|2bfp]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pteridine_reductase Pteridine reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.33 1.5.1.33] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pteridine_reductase Pteridine reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.33 1.5.1.33] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bfa RCSB], [http://www.ebi.ac.uk/pdbsum/2bfa PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfa OCA], [http://pdbe.org/2bfa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bfa RCSB], [http://www.ebi.ac.uk/pdbsum/2bfa PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2bfa" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Leishmania major~~]]		[[Category: Leima]]
	[[Category: Pteridine reductase]]		[[Category: Pteridine reductase]]
	[[Category: Hunter, W N]]		[[Category: Hunter, W N]]

OCA at 15:03, 24 December 2014

2014-12-24T15:03:04Z

← Older revision		Revision as of 15:03, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bfa]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BFA FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bfa]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BFA FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CB3:10-PROPARGYL-5,8-DIDEAZAFOLIC+ACID'>CB3</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CB3:10-PROPARGYL-5,8-DIDEAZAFOLIC+ACID'>CB3</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e7w\|1e7w]], [[1e92\|1e92]], [[1w0c\|1w0c]], [[2bf7\|2bf7]], [[2bfm\|2bfm]], [[2bfo\|2bfo]], [[2bfp\|2bfp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e7w\|1e7w]], [[1e92\|1e92]], [[1w0c\|1w0c]], [[2bf7\|2bf7]], [[2bfm\|2bfm]], [[2bfo\|2bfo]], [[2bfp\|2bfp]]</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pteridine_reductase Pteridine reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.33 1.5.1.33] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pteridine_reductase Pteridine reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.33 1.5.1.33] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bfa RCSB], [http://www.ebi.ac.uk/pdbsum/2bfa PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bfa RCSB], [http://www.ebi.ac.uk/pdbsum/2bfa PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/PTR1_LEIMA PTR1_LEIMA]] Exhibits a NADPH-dependent biopterin reductase activity. Has good activity with folate and significant activity with dihydrofolate and dihydrobiopterin, but not with quinonoid dihydrobiopterin. Confers resistance to methotrexate (MTX).<ref>PMID:7972081</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 37:
	[[Category: Leishmania major]]		[[Category: Leishmania major]]
	[[Category: Pteridine reductase]]		[[Category: Pteridine reductase]]
	[[Category: Hunter, W N.]]		[[Category: Hunter, W N]]
	[[Category: Schuettelkopf, A W.]]		[[Category: Schuettelkopf, A W]]
	[[Category: Drug resistance]]		[[Category: Drug resistance]]
	[[Category: Methotrexate resistance]]		[[Category: Methotrexate resistance]]
	[[Category: Nadp]]		[[Category: Nadp]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	~~[[Category: Pteridine reductase]]~~
	[[Category: Pterin salvage]]		[[Category: Pterin salvage]]
	[[Category: Short-chain dehydrogenase/reductase]]		[[Category: Short-chain dehydrogenase/reductase]]
	[[Category: Trypanosomatid]]		[[Category: Trypanosomatid]]

OCA at 10:38, 3 October 2014

2014-10-03T10:38:43Z

← Older revision		Revision as of 10:38, 3 October 2014
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	~~{{STRUCTURE_2bfa\| PDB=2bfa \| SCENE= }}~~		==LEISHMANIA MAJOR PTERIDINE REDUCTASE 1 IN COMPLEX WITH NADP AND CB3717==
	===LEISHMANIA MAJOR PTERIDINE REDUCTASE 1 IN COMPLEX WITH NADP AND CB3717===		<StructureSection load='2bfa' size='340' side='right' caption='[[2bfa]], [[Resolution\|resolution]] 2.70Å' scene=''>
	~~{{ABSTRACT_PUBMED_16055151}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[2bfa]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BFA FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CB3:10-PROPARGYL-5,8-DIDEAZAFOLIC+ACID'>CB3</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e7w\|1e7w]], [[1e92\|1e92]], [[1w0c\|1w0c]], [[2bf7\|2bf7]], [[2bfm\|2bfm]], [[2bfo\|2bfo]], [[2bfp\|2bfp]]</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pteridine_reductase Pteridine reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.33 1.5.1.33] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bfa RCSB], [http://www.ebi.ac.uk/pdbsum/2bfa PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bf/2bfa_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Pteridine reductase (PTR1) is an NADPH-dependent short-chain reductase found in parasitic trypanosomatid protozoans. The enzyme participates in the salvage of pterins and represents a target for the development of improved therapies for infections caused by these parasites. A series of crystallographic analyses of Leishmania major PTR1 are reported. Structures of the enzyme in a binary complex with the cofactor NADPH, and ternary complexes with cofactor and biopterin, 5,6-dihydrobiopterin, and 5,6,7,8-tetrahydrobiopterin reveal that PTR1 does not undergo any major conformational changes to accomplish binding and processing of substrates, and confirm that these molecules bind in a single orientation at the catalytic center suitable for two distinct reductions. Ternary complexes with cofactor and CB3717 and trimethoprim (TOP), potent inhibitors of thymidylate synthase and dihydrofolate reductase, respectively, have been characterized. The structure with CB3717 reveals that the quinazoline moiety binds in similar fashion to the pterin substrates/products and dominates interactions with the enzyme. In the complex with TOP, steric restrictions enforced on the trimethoxyphenyl substituent prevent the 2,4-diaminopyrimidine moiety from adopting the pterin mode of binding observed in dihydrofolate reductase, and explain the inhibition properties of a range of pyrimidine derivates. The molecular detail provided by these complex structures identifies the important interactions necessary to assist the structure-based development of novel enzyme inhibitors of potential therapeutic value.

	~~==Function==~~		Structures of Leishmania major pteridine reductase complexes reveal the active site features important for ligand binding and to guide inhibitor design.,Schuttelkopf AW, Hardy LW, Beverley SM, Hunter WN J Mol Biol. 2005 Sep 9;352(1):105-16. PMID:16055151<ref>PMID:16055151</ref>
	~~[[http://www.uniprot.org/uniprot/PTR1_LEIMA PTR1_LEIMA]] Exhibits a NADPH-dependent biopterin~~ reductase ~~activity~~. ~~Has good activity with folate and significant activity with dihydrofolate and dihydrobiopterin~~, ~~but not with quinonoid dihydrobiopterin~~. ~~Confers resistance to methotrexate~~ (~~MTX~~).<ref>PMID:~~7972081~~</ref>

	~~==About this Structure==~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~[[2bfa]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Leishmania_major Leishmania major]~~. ~~Full crystallographic information is available from [http:/~~/~~oca.weizmann.ac.il/oca-bin/ocashort?id=2BFA OCA].~~		</div>

	==See Also==		==See Also==
	*[[Pteridine reductase\|Pteridine reductase]]		*[[Pteridine reductase\|Pteridine reductase]]
			== References ==
	==~~Reference~~==		<references/>
	~~<ref group="xtra">PMID:016055151</ref>~~<references ~~group="xtra"~~/><~~references~~/>		__TOC__
			</StructureSection>
	[[Category: Leishmania major]]		[[Category: Leishmania major]]
	[[Category: Pteridine reductase]]		[[Category: Pteridine reductase]]

OCA at 08:21, 23 April 2014

2014-04-23T08:21:04Z

← Older revision		Revision as of 08:21, 23 April 2014
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	~~[[Image:2bfa.png\|left\|200px]]~~

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2bfa", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2bfa\| PDB=2bfa \| SCENE= }}		{{STRUCTURE_2bfa\| PDB=2bfa \| SCENE= }}

	===LEISHMANIA MAJOR PTERIDINE REDUCTASE 1 IN COMPLEX WITH NADP AND CB3717===		===LEISHMANIA MAJOR PTERIDINE REDUCTASE 1 IN COMPLEX WITH NADP AND CB3717===
			{{ABSTRACT_PUBMED_16055151}}

			==Function==
	~~<!--~~		[[http://www.uniprot.org/uniprot/PTR1_LEIMA PTR1_LEIMA]] Exhibits a NADPH-dependent biopterin reductase activity. Has good activity with folate and significant activity with dihydrofolate and dihydrobiopterin, but not with quinonoid dihydrobiopterin. Confers resistance to methotrexate (MTX).<ref>PMID:7972081</ref>
	~~The line below this paragraph, {{ABSTRACT_PUBMED_16055151}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov~~)~~, where 16055151 is the PubMed ID number~~.
	-->
	~~{{ABSTRACT_PUBMED_16055151}}~~

	==About this Structure==		==About this Structure==
	[[2bfa]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFA OCA].		[[2bfa]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFA OCA].

			==See Also==
			*[[Pteridine reductase\|Pteridine reductase]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:016055151</ref><references group="xtra"/>		<ref group="xtra">PMID:016055151</ref><references group="xtra"/><references/>
	[[Category: Leishmania major]]		[[Category: Leishmania major]]
	[[Category: Pteridine reductase]]		[[Category: Pteridine reductase]]

OCA: Protected "2bfa" [edit=sysop:move=sysop]

2012-06-02T09:08:28Z

Protected "2bfa" [edit=sysop:move=sysop]

← Older revision	Revision as of 09:08, 2 June 2012
(No difference)