2bfv - Revision history

OCA at 07:46, 23 October 2024

2024-10-23T07:46:29Z

← Older revision		Revision as of 07:46, 23 October 2024
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	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bf/2bfv_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bf/2bfv_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/~~initialview01~~.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>

OCA at 06:42, 9 August 2023

2023-08-09T06:42:14Z

← Older revision		Revision as of 06:42, 9 August 2023
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	<StructureSection load='2bfv' size='340' side='right'caption='[[2bfv]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2bfv' size='340' side='right'caption='[[2bfv]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bfv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BFV FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bfv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BFV FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=STG:ESTRIOL+3~~-~~(B-D-GLUCURONIDE)'>STG</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[1bfv\|1bfv]]~~</~~div~~></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=STG:ESTRIOL+3-(B-D-GLUCURONIDE)'>STG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfv OCA], [https://pdbe.org/2bfv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bfv RCSB], [https://www.ebi.ac.uk/pdbsum/2bfv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bfv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfv OCA], [https://pdbe.org/2bfv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bfv RCSB], [https://www.ebi.ac.uk/pdbsum/2bfv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bfv ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/KV2A7_MOUSE KV2A7_MOUSE]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</div>		</div>
	<div class="pdbe-citations 2bfv" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2bfv" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Lk3 transgenic mice~~]]		[[Category: Mus musculus]]
	[[Category: Phillips~~, S E.V~~]]		[[Category: Phillips SEV]]
	[[Category: Trinh~~, C H]]~~		[[Category: Trinh CH]]
	~~[[Category: Fine specificity]]~~
	~~[[Category: Fv fragment]]~~
	~~[[Category: Immunoglobulin]]~~
	~~[[Category: Steroid hormone~~]]

OCA at 13:09, 24 November 2021

2021-11-24T13:09:06Z

← Older revision		Revision as of 13:09, 24 November 2021
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	<StructureSection load='2bfv' size='340' side='right'caption='[[2bfv]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2bfv' size='340' side='right'caption='[[2bfv]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bfv]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2BFV FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bfv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BFV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=STG:ESTRIOL+3-(B-D-GLUCURONIDE)'>STG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=STG:ESTRIOL+3-(B-D-GLUCURONIDE)'>STG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bfv\|1bfv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1bfv\|1bfv]]</div></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2bfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfv OCA], [~~http~~://pdbe.org/2bfv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2bfv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2bfv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2bfv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfv OCA], [https://pdbe.org/2bfv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bfv RCSB], [https://www.ebi.ac.uk/pdbsum/2bfv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bfv ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 08:06, 19 February 2020

2020-02-19T08:06:14Z

← Older revision		Revision as of 08:06, 19 February 2020
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	==MONOCLONAL ANTIBODY FRAGMENT FV4155 FROM E. COLI==		==MONOCLONAL ANTIBODY FRAGMENT FV4155 FROM E. COLI==
	<StructureSection load='2bfv' size='340' side='right' caption='[[2bfv]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2bfv' size='340' side='right'caption='[[2bfv]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bfv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BFV FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bfv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BFV FirstGlance]. <br>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Phillips, S E.V]]		[[Category: Phillips, S E.V]]

OCA at 06:31, 16 May 2018

2018-05-16T06:31:14Z

← Older revision		Revision as of 06:31, 16 May 2018
Line 1:		Line 1:

	==MONOCLONAL ANTIBODY FRAGMENT FV4155 FROM E. COLI==		==MONOCLONAL ANTIBODY FRAGMENT FV4155 FROM E. COLI==
	<StructureSection load='2bfv' size='340' side='right' caption='[[2bfv]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2bfv' size='340' side='right' caption='[[2bfv]], [[Resolution\|resolution]] 2.50Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=STG:ESTRIOL+3-(B-D-GLUCURONIDE)'>STG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=STG:ESTRIOL+3-(B-D-GLUCURONIDE)'>STG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bfv\|1bfv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bfv\|1bfv]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfv OCA], [http://pdbe.org/2bfv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bfv RCSB], [http://www.ebi.ac.uk/pdbsum/2bfv PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfv OCA], [http://pdbe.org/2bfv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bfv RCSB], [http://www.ebi.ac.uk/pdbsum/2bfv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2bfv ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bf/2bfv_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bf/2bfv_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 13:22, 9 February 2016

2016-02-09T13:22:09Z

← Older revision		Revision as of 13:22, 9 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bfv ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:38, 10 September 2015

2015-09-10T22:38:39Z

← Older revision		Revision as of 22:38, 10 September 2015
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	<StructureSection load='2bfv' size='340' side='right' caption='[[2bfv]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2bfv' size='340' side='right' caption='[[2bfv]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bfv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BFV FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bfv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BFV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=STG:ESTRIOL+3-(B-D-GLUCURONIDE)'>STG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=STG:ESTRIOL+3-(B-D-GLUCURONIDE)'>STG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bfv\|1bfv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bfv\|1bfv]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bfv RCSB], [http://www.ebi.ac.uk/pdbsum/2bfv PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfv OCA], [http://pdbe.org/2bfv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bfv RCSB], [http://www.ebi.ac.uk/pdbsum/2bfv PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2bfv" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Mus musculus~~]]		[[Category: Lk3 transgenic mice]]
	[[Category: Phillips, S E.V]]		[[Category: Phillips, S E.V]]
	[[Category: Trinh, C H]]		[[Category: Trinh, C H]]

OCA at 09:21, 8 January 2015

2015-01-08T09:21:57Z

← Older revision		Revision as of 09:21, 8 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2bfv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BFV FirstGlance]. <br>		<table><tr><td colspan='2'>[[2bfv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BFV FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=STG:ESTRIOL+3-(B-D-GLUCURONIDE)'>STG</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=STG:ESTRIOL+3-(B-D-GLUCURONIDE)'>STG</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bfv\|1bfv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bfv\|1bfv]]</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bfv RCSB], [http://www.ebi.ac.uk/pdbsum/2bfv PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bfv RCSB], [http://www.ebi.ac.uk/pdbsum/2bfv PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Phillips, S E.V.]]		[[Category: Phillips, S E.V]]
	[[Category: Trinh, C H.]]		[[Category: Trinh, C H]]
	[[Category: Fine specificity]]		[[Category: Fine specificity]]
	[[Category: Fv fragment]]		[[Category: Fv fragment]]
	[[Category: Immunoglobulin]]		[[Category: Immunoglobulin]]
	[[Category: Steroid hormone]]		[[Category: Steroid hormone]]

OCA at 00:10, 30 September 2014

2014-09-30T00:10:33Z

← Older revision		Revision as of 00:10, 30 September 2014
Line 1:		Line 1:
	[[Image:~~2bfv~~.~~png~~\|~~left~~\|~~200px~~]]		==MONOCLONAL ANTIBODY FRAGMENT FV4155 FROM E. COLI==
			<StructureSection load='2bfv' size='340' side='right' caption='[[2bfv]], [[Resolution\|resolution]] 2.50Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2bfv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BFV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BFV FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=STG:ESTRIOL+3-(B-D-GLUCURONIDE)'>STG</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bfv\|1bfv]]</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bfv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bfv RCSB], [http://www.ebi.ac.uk/pdbsum/2bfv PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bf/2bfv_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			BACKGROUND: The concentration of steroid glucuronides in serial samples of early morning urine (EMU) can be used to predict the fertile period in the female menstrual cycle. The monoclonal antibody 4155 has been used as a convenient means of measuring the concentration of steroid glucuronides in EMU, as it specifically recognises the steroid hormone estrone beta-D-glucuronide (E3G), with very high affinity, and the closely related hormone estriol 3-(beta-d-glucuronide) (EI3G), with reduced affinity. Although 4115 binds these hormones with different affinities, EI3G differs from E3G only in the addition of a hydroxyl group and reduction of an adjacent carbonyl. To investigate the structural basis of this fine binding specificity, we have determined the crystal structures of the variable fragment (Fv) of 4155 in complex with each of these hormones. RESULTS: Two crystal forms of the Fv4155-EI3G complex, at resolutions of 2.1 A and 2.5 A, and one form of the Fv4155-E3G complex, at 2.1 A resolution were solved and refined. The crystal structures show the E3G or EI3G antigen lying in an extended cleft, running form the centre of the antibody combining site down one side of the variable domain interface, and formed almost entirely from residues in the heavy chain. The binding cleft lies primarily between the heavy chain complementarity determining regions (CDRs), rather than in the interface between the heavy and light chains. In both complexes the binding of the glucuronic sugar, and rings A and B of the steroid, is specified by the shape of the narrow cleft. Analysis of the Fv structure reveals that five of the six CDR regions can be assigned to one of the predefined canonical structural classes. CONCLUSIONS: The difference in the binding affinity of Fv4155 for the two steroid hormones is accounted for by a subtle combination of a less favoured hydrogen-bond geometry, and a minor rearrangement of the water molecule network around the binding site. The rearrangement of water molecules results from the burial of the additional hydroxyl group of the EI3G in a hydrophobic environment.

	~~{{STRUCTURE_2bfv\| PDB=2bfv \| SCENE= }}~~		Antibody fragment Fv4155 bound to two closely related steroid hormones: the structural basis of fine specificity.,Trinh CH, Hemmington SD, Verhoeyen ME, Phillips SE Structure. 1997 Jul 15;5(7):937-48. PMID:9261086<ref>PMID:9261086</ref>

	~~===MONOCLONAL ANTIBODY FRAGMENT FV4155 FROM E. COLI===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_9261086}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2bfv]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2BFV OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:009261086<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Phillips, S E.V.]]		[[Category: Phillips, S E.V.]]

OCA: Protected "2bfv" [edit=sysop:move=sysop]

2013-01-06T09:36:10Z

Protected "2bfv" [edit=sysop:move=sysop]

← Older revision	Revision as of 09:36, 6 January 2013
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