2blc - Revision history

OCA at 09:17, 9 May 2024

2024-05-09T09:17:28Z

← Older revision		Revision as of 09:17, 9 May 2024
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	<StructureSection load='2blc' size='340' side='right'caption='[[2blc]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='2blc' size='340' side='right'caption='[[2blc]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2blc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Haemamoeba_vivax Haemamoeba~~ vivax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BLC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BLC FirstGlance]. <br>		<table><tr><td colspan='2'>[[2blc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_vivax Plasmodium vivax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BLC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BLC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CP7:6-ETHYL-5-PHENYLPYRIMIDINE-2,4-DIAMINE'>CP7</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene~~></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.25Å</td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2bl9\|2bl9]], [[2bla\|2bla]], [[2blb\|2blb]]</div></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CP7:6-ETHYL-5-PHENYLPYRIMIDINE-2,4-DIAMINE'>CP7</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2blc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2blc OCA], [https://pdbe.org/2blc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2blc RCSB], [https://www.ebi.ac.uk/pdbsum/2blc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2blc ProSAT], [https://www.topsan.org/Proteins/RSGI/2blc TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2blc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2blc OCA], [https://pdbe.org/2blc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2blc RCSB], [https://www.ebi.ac.uk/pdbsum/2blc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2blc ProSAT], [https://www.topsan.org/Proteins/RSGI/2blc TOPSAN]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/DRTS_PLAVI DRTS_PLAVI] Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Dihydrofolate reductase]]~~
	~~[[Category: Haemamoeba vivax]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	~~[[Category: Chitnumsub, P]]~~
	~~[[Category: Khongsuk, P]]~~
	~~[[Category: Kongsaeree, P]]~~
	~~[[Category: Leartsakulpanich, U]]~~
	~~[[Category: Tarnchompoo, B]]~~
	~~[[Category: Walkinshaw, M D]]~~
	~~[[Category: Yuthavong, Y]]~~
	~~[[Category: Double mutant]]~~
	~~[[Category: Drug resistance]]~~
	~~[[Category: Malaria]]~~
	~~[[Category: Oxidoreductase]]~~
	[[Category: Plasmodium vivax]]		[[Category: Plasmodium vivax]]
	[[Category: ~~Pyrimethamine~~]]		[[Category: Chitnumsub P]]
	[[Category: ~~Thymidylate synthase~~]]		[[Category: Khongsuk P]]
			[[Category: Kongsaeree P]]
			[[Category: Leartsakulpanich U]]
			[[Category: Tarnchompoo B]]
			[[Category: Walkinshaw MD]]
			[[Category: Yuthavong Y]]

OCA at 07:10, 24 February 2021

2021-02-24T07:10:35Z

← Older revision		Revision as of 07:10, 24 February 2021
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	<StructureSection load='2blc' size='340' side='right'caption='[[2blc]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='2blc' size='340' side='right'caption='[[2blc]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2blc]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Haemamoeba_vivax Haemamoeba vivax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BLC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2BLC FirstGlance]. <br>		<table><tr><td colspan='2'>[[2blc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemamoeba_vivax Haemamoeba vivax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BLC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BLC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CP7:6-ETHYL-5-PHENYLPYRIMIDINE-2,4-DIAMINE'>CP7</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CP7:6-ETHYL-5-PHENYLPYRIMIDINE-2,4-DIAMINE'>CP7</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bl9\|2bl9]], [[2bla\|2bla]], [[2blb\|2blb]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2bl9\|2bl9]], [[2bla\|2bla]], [[2blb\|2blb]]</div></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2blc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2blc OCA], [~~http~~://pdbe.org/2blc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2blc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2blc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2blc ProSAT], [~~http~~://www.topsan.org/Proteins/RSGI/2blc TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2blc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2blc OCA], [https://pdbe.org/2blc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2blc RCSB], [https://www.ebi.ac.uk/pdbsum/2blc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2blc ProSAT], [https://www.topsan.org/Proteins/RSGI/2blc TOPSAN]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	==See Also==		==See Also==
	*[[Dihydrofolate reductase\|Dihydrofolate reductase]]		*[[Dihydrofolate reductase 3D structures\|Dihydrofolate reductase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:10, 23 May 2019

2019-05-23T07:10:30Z

← Older revision		Revision as of 07:10, 23 May 2019
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	==SP21 double mutant P. vivax Dihydrofolate reductase in complex with des-chloropyrimethamine==		==SP21 double mutant P. vivax Dihydrofolate reductase in complex with des-chloropyrimethamine==
	<StructureSection load='2blc' size='340' side='right' caption='[[2blc]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='2blc' size='340' side='right'caption='[[2blc]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2blc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemamoeba_vivax Haemamoeba vivax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BLC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BLC FirstGlance]. <br>		<table><tr><td colspan='2'>[[2blc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemamoeba_vivax Haemamoeba vivax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BLC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BLC FirstGlance]. <br>
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	[[Category: Dihydrofolate reductase]]		[[Category: Dihydrofolate reductase]]
	[[Category: Haemamoeba vivax]]		[[Category: Haemamoeba vivax]]
			[[Category: Large Structures]]
	[[Category: Chitnumsub, P]]		[[Category: Chitnumsub, P]]
	[[Category: Khongsuk, P]]		[[Category: Khongsuk, P]]

OCA at 06:13, 16 May 2018

2018-05-16T06:13:20Z

← Older revision		Revision as of 06:13, 16 May 2018
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	==SP21 ~~DOUBLE MUTANT~~ P. ~~VIVAX DIHYDROFOLATE REDUCTASE IN COMPLEX WITH DES~~-~~CHLOROPYRIMETHAMINE~~==
			==SP21 double mutant P. vivax Dihydrofolate reductase in complex with des-chloropyrimethamine==
	<StructureSection load='2blc' size='340' side='right' caption='[[2blc]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='2blc' size='340' side='right' caption='[[2blc]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2blc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Plavi Plavi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BLC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BLC FirstGlance]. <br>		<table><tr><td colspan='2'>[[2blc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemamoeba_vivax Haemamoeba vivax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BLC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BLC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CP7:6-ETHYL-5-PHENYLPYRIMIDINE-2,4-DIAMINE'>CP7</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CP7:6-ETHYL-5-PHENYLPYRIMIDINE-2,4-DIAMINE'>CP7</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bl9\|2bl9]], [[2bla\|2bla]], [[2blb\|2blb]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bl9\|2bl9]], [[2bla\|2bla]], [[2blb\|2blb]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2blc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2blc OCA], [http://pdbe.org/2blc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2blc RCSB], [http://www.ebi.ac.uk/pdbsum/2blc PDBsum], [http://www.topsan.org/Proteins/RSGI/2blc TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2blc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2blc OCA], [http://pdbe.org/2blc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2blc RCSB], [http://www.ebi.ac.uk/pdbsum/2blc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2blc ProSAT], [http://www.topsan.org/Proteins/RSGI/2blc TOPSAN]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bl/2blc_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bl/2blc_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</StructureSection>		</StructureSection>
	[[Category: Dihydrofolate reductase]]		[[Category: Dihydrofolate reductase]]
	[[Category: ~~Plavi~~]]		[[Category: Haemamoeba vivax]]
	[[Category: Chitnumsub, P]]		[[Category: Chitnumsub, P]]
	[[Category: Khongsuk, P]]		[[Category: Khongsuk, P]]

OCA at 20:09, 7 February 2016

2016-02-07T20:09:57Z

← Older revision		Revision as of 20:09, 7 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2blc ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 04:36, 10 September 2015

2015-09-10T04:36:59Z

← Older revision		Revision as of 04:36, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2blc' size='340' side='right' caption='[[2blc]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='2blc' size='340' side='right' caption='[[2blc]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2blc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Plasmodium_vivax Plasmodium vivax~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BLC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BLC FirstGlance]. <br>		<table><tr><td colspan='2'>[[2blc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plavi Plavi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BLC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BLC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CP7:6-ETHYL-5-PHENYLPYRIMIDINE-2,4-DIAMINE'>CP7</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CP7:6-ETHYL-5-PHENYLPYRIMIDINE-2,4-DIAMINE'>CP7</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bl9\|2bl9]], [[2bla\|2bla]], [[2blb\|2blb]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bl9\|2bl9]], [[2bla\|2bla]], [[2blb\|2blb]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2blc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2blc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2blc RCSB], [http://www.ebi.ac.uk/pdbsum/2blc PDBsum], [http://www.topsan.org/Proteins/RSGI/2blc TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2blc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2blc OCA], [http://pdbe.org/2blc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2blc RCSB], [http://www.ebi.ac.uk/pdbsum/2blc PDBsum], [http://www.topsan.org/Proteins/RSGI/2blc TOPSAN]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2blc" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Dihydrofolate reductase]]		[[Category: Dihydrofolate reductase]]
	[[Category: ~~Plasmodium vivax~~]]		[[Category: Plavi]]
	[[Category: Chitnumsub, P]]		[[Category: Chitnumsub, P]]
	[[Category: Khongsuk, P]]		[[Category: Khongsuk, P]]
Line 46:		Line 47:
	[[Category: Malaria]]		[[Category: Malaria]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
			[[Category: Plasmodium vivax]]
	[[Category: Pyrimethamine]]		[[Category: Pyrimethamine]]
	[[Category: Thymidylate synthase]]		[[Category: Thymidylate synthase]]

OCA at 09:57, 8 January 2015

2015-01-08T09:57:58Z

← Older revision		Revision as of 09:57, 8 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2blc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plasmodium_vivax Plasmodium vivax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BLC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BLC FirstGlance]. <br>		<table><tr><td colspan='2'>[[2blc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plasmodium_vivax Plasmodium vivax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BLC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BLC FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CP7:6-ETHYL-5-PHENYLPYRIMIDINE-2,4-DIAMINE'>CP7</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CP7:6-ETHYL-5-PHENYLPYRIMIDINE-2,4-DIAMINE'>CP7</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bl9\|2bl9]], [[2bla\|2bla]], [[2blb\|2blb]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bl9\|2bl9]], [[2bla\|2bla]], [[2blb\|2blb]]</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2blc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2blc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2blc RCSB], [http://www.ebi.ac.uk/pdbsum/2blc PDBsum], [http://www.topsan.org/Proteins/RSGI/2blc TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2blc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2blc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2blc RCSB], [http://www.ebi.ac.uk/pdbsum/2blc PDBsum], [http://www.topsan.org/Proteins/RSGI/2blc TOPSAN]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Dihydrofolate reductase]]		[[Category: Dihydrofolate reductase]]
	[[Category: Plasmodium vivax]]		[[Category: Plasmodium vivax]]
	[[Category: Chitnumsub, P.]]		[[Category: Chitnumsub, P]]
	[[Category: Khongsuk, P.]]		[[Category: Khongsuk, P]]
	[[Category: Kongsaeree, P.]]		[[Category: Kongsaeree, P]]
	[[Category: Leartsakulpanich, U.]]		[[Category: Leartsakulpanich, U]]
	[[Category: Tarnchompoo, B.]]		[[Category: Tarnchompoo, B]]
	[[Category: Walkinshaw, M D.]]		[[Category: Walkinshaw, M D]]
	[[Category: Yuthavong, Y~~.]]~~		[[Category: Yuthavong, Y]]
	~~[[Category: Dihydrofolate reductase~~]]
	[[Category: Double mutant]]		[[Category: Double mutant]]
	[[Category: Drug resistance]]		[[Category: Drug resistance]]
	[[Category: Malaria]]		[[Category: Malaria]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	~~[[Category: Plasmodium vivax]]~~
	[[Category: Pyrimethamine]]		[[Category: Pyrimethamine]]
	[[Category: Thymidylate synthase]]		[[Category: Thymidylate synthase]]

OCA at 02:46, 29 September 2014

2014-09-29T02:46:51Z

← Older revision		Revision as of 02:46, 29 September 2014
Line 1:		Line 1:
	[[Image:~~2blc~~.~~png~~\|~~left~~\|~~200px~~]]		==SP21 DOUBLE MUTANT P. VIVAX DIHYDROFOLATE REDUCTASE IN COMPLEX WITH DES-CHLOROPYRIMETHAMINE==
			<StructureSection load='2blc' size='340' side='right' caption='[[2blc]], [[Resolution\|resolution]] 2.25Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2blc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plasmodium_vivax Plasmodium vivax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BLC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BLC FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CP7:6-ETHYL-5-PHENYLPYRIMIDINE-2,4-DIAMINE'>CP7</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bl9\|2bl9]], [[2bla\|2bla]], [[2blb\|2blb]]</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2blc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2blc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2blc RCSB], [http://www.ebi.ac.uk/pdbsum/2blc PDBsum], [http://www.topsan.org/Proteins/RSGI/2blc TOPSAN]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bl/2blc_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Pyrimethamine (Pyr) targets dihydrofolate reductase of Plasmodium vivax (PvDHFR) as well as other malarial parasites, but its use as antimalarial is hampered by the widespread high resistance. Comparison of the crystal structures of PvDHFR from wild-type and the Pyr-resistant (SP21, Ser-58 --> Arg + Ser-117 --> Asn) strain as complexes with NADPH and Pyr or its analog lacking p-Cl (Pyr20) clearly shows that the steric conflict arising from the side chain of Asn-117 in the mutant enzyme, accompanied by the loss of binding to Ser-120, is mainly responsible for the reduction in binding of Pyr. Pyr20 still effectively inhibits both the wild-type and SP21 proteins, and the x-ray structures of these complexes show how Pyr20 fits into both active sites without steric strain. These structural insights suggest a general approach for developing new generations of antimalarial DHFR inhibitors that, by only occupying substrate space of the active site, would retain binding affinity with the mutant enzymes.

	~~{{STRUCTURE_2blc\| PDB=2blc \| SCENE= }}~~		Crystal structure of dihydrofolate reductase from Plasmodium vivax: pyrimethamine displacement linked with mutation-induced resistance.,Kongsaeree P, Khongsuk P, Leartsakulpanich U, Chitnumsub P, Tarnchompoo B, Walkinshaw MD, Yuthavong Y Proc Natl Acad Sci U S A. 2005 Sep 13;102(37):13046-51. Epub 2005 Aug 31. PMID:16135570<ref>PMID:16135570</ref>

	~~===SP21 DOUBLE MUTANT P. VIVAX DIHYDROFOLATE REDUCTASE IN COMPLEX WITH DES-CHLOROPYRIMETHAMINE===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_16135570}}~~

	~~==About this Structure==~~
	~~[[2blc]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Plasmodium_vivax Plasmodium vivax]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2BLC OCA].~~

	==See Also==		==See Also==
	*[[Dihydrofolate reductase\|Dihydrofolate reductase]]		*[[Dihydrofolate reductase\|Dihydrofolate reductase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:016135570<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Dihydrofolate reductase]]		[[Category: Dihydrofolate reductase]]
	[[Category: Plasmodium vivax]]		[[Category: Plasmodium vivax]]

OCA at 07:44, 20 October 2012

2012-10-20T07:44:11Z

← Older revision		Revision as of 07:44, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[2blc]] is a 1 chain structure ~~of [[Dihydrofolate reductase]]~~ with sequence from [http://en.wikipedia.org/wiki/Plasmodium_vivax Plasmodium vivax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BLC OCA].		[[2blc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plasmodium_vivax Plasmodium vivax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BLC OCA].

	==See Also==		==See Also==

OCA at 17:16, 25 July 2012

2012-07-25T17:16:49Z

← Older revision		Revision as of 17:16, 25 July 2012
Line 1:		Line 1:
	[[Image:2blc.png\|left\|200px]]		[[Image:2blc.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2blc", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2blc\| PDB=2blc \| SCENE= }}		{{STRUCTURE_2blc\| PDB=2blc \| SCENE= }}

	===SP21 DOUBLE MUTANT P. VIVAX DIHYDROFOLATE REDUCTASE IN COMPLEX WITH DES-CHLOROPYRIMETHAMINE===		===SP21 DOUBLE MUTANT P. VIVAX DIHYDROFOLATE REDUCTASE IN COMPLEX WITH DES-CHLOROPYRIMETHAMINE===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_16135570}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 16135570 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_16135570}}		{{ABSTRACT_PUBMED_16135570}}

Line 22:		Line 11:

	==See Also==		==See Also==
	*[[Dihydrofolate reductase]]		*[[Dihydrofolate reductase\|Dihydrofolate reductase]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~16135570~~</ref><references group="xtra"/>		<ref group="xtra">PMID:016135570</ref><references group="xtra"/>
	[[Category: Dihydrofolate reductase]]		[[Category: Dihydrofolate reductase]]
	[[Category: Plasmodium vivax]]		[[Category: Plasmodium vivax]]