2c8m - Revision history

OCA at 14:09, 13 December 2023

2023-12-13T14:09:07Z

← Older revision		Revision as of 14:09, 13 December 2023
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	<StructureSection load='2c8m' size='340' side='right'caption='[[2c8m]], [[Resolution\|resolution]] 1.89Å' scene=''>		<StructureSection load='2c8m' size='340' side='right'caption='[[2c8m]], [[Resolution\|resolution]] 1.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2c8m]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Theac Theac~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C8M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C8M FirstGlance]. <br>		<table><tr><td colspan='2'>[[2c8m]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoplasma_acidophilum_DSM_1728 Thermoplasma acidophilum DSM 1728]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C8M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C8M FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=LPA:LIPOIC+ACID'>LPA</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.89Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[2ars\|2ars]], [[2art\|2art]], [[2aru\|2aru]], [[2c7i\|2c7i]]~~</~~div~~></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LPA:LIPOIC+ACID'>LPA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c8m OCA], [https://pdbe.org/2c8m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c8m RCSB], [https://www.ebi.ac.uk/pdbsum/2c8m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c8m ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c8m OCA], [https://pdbe.org/2c8m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c8m RCSB], [https://www.ebi.ac.uk/pdbsum/2c8m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c8m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/LPLAN_THEAC LPLAN_THEAC]] Part of a lipoate-protein ligase complex that catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes. Can also use octanoate as substrate.<ref>PMID:16141198</ref> <ref>PMID:19520844</ref> <ref>PMID:19594830</ref>		[https://www.uniprot.org/uniprot/LPLAN_THEAC LPLAN_THEAC] Part of a lipoate-protein ligase complex that catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes. Can also use octanoate as substrate.<ref>PMID:16141198</ref> <ref>PMID:19520844</ref> <ref>PMID:19594830</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Theac~~]]		[[Category: Thermoplasma acidophilum DSM 1728]]
	[[Category: Luisi~~, B F~~]]		[[Category: Luisi BF]]
	[[Category: McManus, E]]		[[Category: McManus E]]
	[[Category: Perham~~, R N]]~~		[[Category: Perham RN]]
	~~[[Category: Ligase]]~~
	~~[[Category: Lipoylation~~]]

OCA at 20:33, 20 October 2021

2021-10-20T20:33:39Z

← Older revision		Revision as of 20:33, 20 October 2021
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	<StructureSection load='2c8m' size='340' side='right'caption='[[2c8m]], [[Resolution\|resolution]] 1.89Å' scene=''>		<StructureSection load='2c8m' size='340' side='right'caption='[[2c8m]], [[Resolution\|resolution]] 1.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2c8m]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Theac Theac]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C8M OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2C8M FirstGlance]. <br>		<table><tr><td colspan='2'>[[2c8m]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Theac Theac]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C8M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C8M FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LPA:LIPOIC+ACID'>LPA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LPA:LIPOIC+ACID'>LPA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ars\|2ars]], [[2art\|2art]], [[2aru\|2aru]], [[2c7i\|2c7i]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ars\|2ars]], [[2art\|2art]], [[2aru\|2aru]], [[2c7i\|2c7i]]</div></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2c8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c8m OCA], [~~http~~://pdbe.org/2c8m PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2c8m RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2c8m PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2c8m ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c8m OCA], [https://pdbe.org/2c8m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c8m RCSB], [https://www.ebi.ac.uk/pdbsum/2c8m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c8m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/LPLAN_THEAC LPLAN_THEAC]] Part of a lipoate-protein ligase complex that catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes. Can also use octanoate as substrate.<ref>PMID:16141198</ref> <ref>PMID:19520844</ref> <ref>PMID:19594830</ref>		[[https://www.uniprot.org/uniprot/LPLAN_THEAC LPLAN_THEAC]] Part of a lipoate-protein ligase complex that catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes. Can also use octanoate as substrate.<ref>PMID:16141198</ref> <ref>PMID:19520844</ref> <ref>PMID:19594830</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 05:36, 10 October 2019

2019-10-10T05:36:30Z

← Older revision		Revision as of 05:36, 10 October 2019
Line 1:		Line 1:

	==Structure of protein Ta0514, putative lipoate protein ligase from T. acidophilum with bound lipoic acid==		==Structure of protein Ta0514, putative lipoate protein ligase from T. acidophilum with bound lipoic acid==
	<StructureSection load='2c8m' size='340' side='right' caption='[[2c8m]], [[Resolution\|resolution]] 1.89Å' scene=''>		<StructureSection load='2c8m' size='340' side='right'caption='[[2c8m]], [[Resolution\|resolution]] 1.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2c8m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Theac Theac]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C8M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C8M FirstGlance]. <br>		<table><tr><td colspan='2'>[[2c8m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Theac Theac]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C8M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C8M FirstGlance]. <br>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Theac]]		[[Category: Theac]]
	[[Category: Luisi, B F]]		[[Category: Luisi, B F]]

OCA at 08:10, 23 May 2018

2018-05-23T08:10:15Z

← Older revision		Revision as of 08:10, 23 May 2018
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	==~~STRUCTURE OF PROTEIN TA0514~~, ~~PUTATIVE LIPOATE PROTEIN LIGASE FROM~~ T. ~~ACIDOPHILUM WITH BOUND LIPOIC ACID~~==
			==Structure of protein Ta0514, putative lipoate protein ligase from T. acidophilum with bound lipoic acid==
	<StructureSection load='2c8m' size='340' side='right' caption='[[2c8m]], [[Resolution\|resolution]] 1.89Å' scene=''>		<StructureSection load='2c8m' size='340' side='right' caption='[[2c8m]], [[Resolution\|resolution]] 1.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LPA:LIPOIC+ACID'>LPA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LPA:LIPOIC+ACID'>LPA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ars\|2ars]], [[2art\|2art]], [[2aru\|2aru]], [[2c7i\|2c7i]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ars\|2ars]], [[2art\|2art]], [[2aru\|2aru]], [[2c7i\|2c7i]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c8m OCA], [http://pdbe.org/2c8m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2c8m RCSB], [http://www.ebi.ac.uk/pdbsum/2c8m PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c8m OCA], [http://pdbe.org/2c8m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2c8m RCSB], [http://www.ebi.ac.uk/pdbsum/2c8m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2c8m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c8/2c8m_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c8/2c8m_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	[[Category: Theac]]		[[Category: Theac]]
	[[Category: Luisi, B F]]		[[Category: Luisi, B F]]
	[[Category: ~~Mcmanus~~, E]]		[[Category: McManus, E]]
	[[Category: Perham, R N]]		[[Category: Perham, R N]]
	[[Category: Ligase]]		[[Category: Ligase]]
	[[Category: Lipoylation]]		[[Category: Lipoylation]]

OCA at 18:34, 9 February 2016

2016-02-09T18:34:49Z

← Older revision		Revision as of 18:34, 9 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2c8m ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 00:56, 11 September 2015

2015-09-11T00:56:21Z

← Older revision		Revision as of 00:56, 11 September 2015
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	<StructureSection load='2c8m' size='340' side='right' caption='[[2c8m]], [[Resolution\|resolution]] 1.89Å' scene=''>		<StructureSection load='2c8m' size='340' side='right' caption='[[2c8m]], [[Resolution\|resolution]] 1.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2c8m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermoplasma_acidophilum Thermoplasma acidophilum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C8M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C8M FirstGlance]. <br>		<table><tr><td colspan='2'>[[2c8m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Theac Theac]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C8M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C8M FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LPA:LIPOIC+ACID'>LPA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LPA:LIPOIC+ACID'>LPA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ars\|2ars]], [[2art\|2art]], [[2aru\|2aru]], [[2c7i\|2c7i]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ars\|2ars]], [[2art\|2art]], [[2aru\|2aru]], [[2c7i\|2c7i]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c8m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2c8m RCSB], [http://www.ebi.ac.uk/pdbsum/2c8m PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c8m OCA], [http://pdbe.org/2c8m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2c8m RCSB], [http://www.ebi.ac.uk/pdbsum/2c8m PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/~~LPLA_THEAC LPLA_THEAC~~]] Part of a lipoate-protein ligase complex that catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes. Can also use octanoate as substrate.<ref>PMID:~~16384580</ref> <ref>PMID:19594830~~</ref> <ref>PMID:19520844</ref> <ref>PMID:~~16141198~~</ref>		[[http://www.uniprot.org/uniprot/LPLAN_THEAC LPLAN_THEAC]] Part of a lipoate-protein ligase complex that catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes. Can also use octanoate as substrate.<ref>PMID:16141198</ref> <ref>PMID:19520844</ref> <ref>PMID:19594830</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2c8m" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Thermoplasma acidophilum~~]]		[[Category: Theac]]
	[[Category: Luisi, B F]]		[[Category: Luisi, B F]]
	[[Category: Mcmanus, E]]		[[Category: Mcmanus, E]]

OCA at 08:42, 25 December 2014

2014-12-25T08:42:58Z

← Older revision		Revision as of 08:42, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2c8m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C8M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C8M FirstGlance]. <br>		<table><tr><td colspan='2'>[[2c8m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C8M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C8M FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LPA:LIPOIC+ACID'>LPA</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LPA:LIPOIC+ACID'>LPA</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ars\|2ars]], [[2art\|2art]], [[2aru\|2aru]], [[2c7i\|2c7i]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ars\|2ars]], [[2art\|2art]], [[2aru\|2aru]], [[2c7i\|2c7i]]</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c8m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2c8m RCSB], [http://www.ebi.ac.uk/pdbsum/2c8m PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c8m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2c8m RCSB], [http://www.ebi.ac.uk/pdbsum/2c8m PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/LPLA_THEAC LPLA_THEAC]] Part of a lipoate-protein ligase complex that catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes. Can also use octanoate as substrate.<ref>PMID:16384580</ref> <ref>PMID:19594830</ref> <ref>PMID:19520844</ref> <ref>PMID:16141198</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Thermoplasma acidophilum]]		[[Category: Thermoplasma acidophilum]]
	[[Category: Luisi, B F.]]		[[Category: Luisi, B F]]
	[[Category: Mcmanus, E.]]		[[Category: Mcmanus, E]]
	[[Category: Perham, R N.]]		[[Category: Perham, R N]]
	[[Category: Ligase]]		[[Category: Ligase]]
	[[Category: Lipoylation]]		[[Category: Lipoylation]]

OCA at 09:50, 3 October 2014

2014-10-03T09:50:31Z

← Older revision		Revision as of 09:50, 3 October 2014
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	~~{{STRUCTURE_2c8m\| PDB=2c8m \| SCENE= }}~~		==STRUCTURE OF PROTEIN TA0514, PUTATIVE LIPOATE PROTEIN LIGASE FROM T. ACIDOPHILUM WITH BOUND LIPOIC ACID==
	===STRUCTURE OF PROTEIN TA0514, PUTATIVE LIPOATE PROTEIN LIGASE FROM T. ACIDOPHILUM WITH BOUND LIPOIC ACID===		<StructureSection load='2c8m' size='340' side='right' caption='[[2c8m]], [[Resolution\|resolution]] 1.89Å' scene=''>
	~~{{ABSTRACT_PUBMED_16384580}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[2c8m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C8M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C8M FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LPA:LIPOIC+ACID'>LPA</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ars\|2ars]], [[2art\|2art]], [[2aru\|2aru]], [[2c7i\|2c7i]]</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c8m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2c8m RCSB], [http://www.ebi.ac.uk/pdbsum/2c8m PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c8/2c8m_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Lipoyl-lysine swinging arms are crucial to the reactions catalysed by the 2-oxo acid dehydrogenase multienzyme complexes. A gene encoding a putative lipoate protein ligase (LplA) of Thermoplasma acidophilum was cloned and expressed in Escherichia coli. The recombinant protein, a monomer of molecular mass 29 kDa, was catalytically inactive. Crystal structures in the absence and presence of bound lipoic acid were solved at 2.1 A resolution. The protein was found to fall into the alpha/beta class and to be structurally homologous to the catalytic domains of class II aminoacyl-tRNA synthases and biotin protein ligase, BirA. Lipoic acid in LplA was bound in the same position as biotin in BirA. The structure of the T.acidophilum LplA and limited proteolysis of E.coli LplA together highlighted some key features of the post-translational modification. A loop comprising residues 71-79 in the T.acidophilum ligase is proposed as interacting with the dithiolane ring of lipoic acid and discriminating against the entry of biotin. A second loop comprising residues 179-193 was disordered in the T.acidophilum structure; tryptic cleavage of the corresponding loop in the E.coli LplA under non-denaturing conditions rendered the enzyme catalytically inactive, emphasizing its importance. The putative LplA of T.acidophilum lacks a C-terminal domain found in its counterparts in E.coli (Gram-negative) or Streptococcus pneumoniae (Gram-positive). A gene encoding a protein that appears to have structural homology to the additional domain in the E.coli and S.pneumoniae enzymes was detected alongside the structural gene encoding the putative LplA in the T.acidophilum genome. It is likely that this protein is required to confer activity on the LplA as currently purified, one protein perhaps catalysing the formation of the obligatory lipoyl-AMP intermediate, and the other transferring the lipoyl group from it to the specific lysine residue in the target protein.

	~~==Function==~~		Structure of a putative lipoate protein ligase from Thermoplasma acidophilum and the mechanism of target selection for post-translational modification.,McManus E, Luisi BF, Perham RN J Mol Biol. 2006 Feb 24;356(3):625-37. Epub 2005 Dec 5. PMID:16384580<ref>PMID:16384580</ref>
	~~[[http://www.uniprot.org/uniprot/LPLA_THEAC LPLA_THEAC]] Part~~ of a lipoate-protein ligase ~~complex that catalyzes both~~ the ~~ATP-dependent activation~~ of ~~exogenously supplied lipoate to lipoyl~~-~~AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate~~-~~dependent enzymes~~. ~~Can also use octanoate as substrate~~.~~<ref>~~PMID:16384580~~</ref>~~ <ref>PMID:~~19594830</ref> <ref>PMID:19520844</ref> <ref>PMID:16141198~~</ref>

	~~==About this Structure==~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~[[2c8m]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2C8M OCA].~~		</div>
			== References ==
	==~~Reference~~==		<references/>
	~~<ref group="xtra">PMID:016384580</ref>~~<references ~~group="xtra"~~/><~~references~~/>		__TOC__
			</StructureSection>
	[[Category: Thermoplasma acidophilum]]		[[Category: Thermoplasma acidophilum]]
	[[Category: Luisi, B F.]]		[[Category: Luisi, B F.]]

OCA at 08:35, 23 April 2014

2014-04-23T08:35:35Z

← Older revision		Revision as of 08:35, 23 April 2014
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	~~[[Image:2c8m.png\|left\|200px]]~~

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2c8m", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2c8m\| PDB=2c8m \| SCENE= }}		{{STRUCTURE_2c8m\| PDB=2c8m \| SCENE= }}

	===STRUCTURE OF PROTEIN TA0514, PUTATIVE LIPOATE PROTEIN LIGASE FROM T. ACIDOPHILUM WITH BOUND LIPOIC ACID===		===STRUCTURE OF PROTEIN TA0514, PUTATIVE LIPOATE PROTEIN LIGASE FROM T. ACIDOPHILUM WITH BOUND LIPOIC ACID===
			{{ABSTRACT_PUBMED_16384580}}

			==Function==
	~~<!--~~		[[http://www.uniprot.org/uniprot/LPLA_THEAC LPLA_THEAC]] Part of a lipoate-protein ligase complex that catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes. Can also use octanoate as substrate.<ref>PMID:16384580</ref> <ref>PMID:19594830</ref> <ref>PMID:19520844</ref> <ref>PMID:16141198</ref>
	~~The line below this paragraph, {{ABSTRACT_PUBMED_16384580}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov), where 16384580 is~~ the ~~PubMed ID number.~~
	-->
	~~{{ABSTRACT_PUBMED_16384580}}~~

	==About this Structure==		==About this Structure==
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	==Reference==		==Reference==
	<ref group="xtra">PMID:016384580</ref><references group="xtra"/>		<ref group="xtra">PMID:016384580</ref><references group="xtra"/><references/>
	[[Category: Thermoplasma acidophilum]]		[[Category: Thermoplasma acidophilum]]
	[[Category: Luisi, B F.]]		[[Category: Luisi, B F.]]

OCA: Protected "2c8m" [edit=sysop:move=sysop]

2011-08-24T05:24:52Z

Protected "2c8m" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:24, 24 August 2011
(No difference)