2ca3 - Revision history

OCA at 05:08, 17 October 2024

2024-10-17T05:08:37Z

← Older revision		Revision as of 05:08, 17 October 2024
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	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/~~Q9LA16_STANO Q9LA16_STANO~~] The exact function is not known. Can catalyze the reduction of a variety of substrates like dimethyl sulfoxide, trimethylamine N-oxide, phenylmethyl sulfoxide and L-methionine sulfoxide. Cannot reduce cyclic N-oxides. Shows no activity as sulfite oxidase.[SAAS:SAAS00086612] The exact function is not known. Can catalyze the reduction of a variety of substrates like dimethyl sulfoxide, trimethylamine N-oxide, phenylmethyl sulfoxide and L-methionine sulfoxide. Cannot reduce cyclic N-oxides. Shows no activity as sulfite oxidase (By similarity).		[https://www.uniprot.org/uniprot/Q9LA16_ANCNO Q9LA16_ANCNO]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ca/2ca3_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ca/2ca3_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/~~initialview01~~.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>

OCA at 14:11, 13 December 2023

2023-12-13T14:11:03Z

← Older revision		Revision as of 14:11, 13 December 2023
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	<StructureSection load='2ca3' size='340' side='right'caption='[[2ca3]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2ca3' size='340' side='right'caption='[[2ca3]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ca3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_8093 Atcc 8093~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CA3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CA3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ca3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Starkeya_novella Starkeya novella]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CA3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CA3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=MSS:(MOLYBDOPTERIN-S,S)-OXO-MOLYBDENUM'>MSS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2blf\|2blf]], [[2bpb\|2bpb]], [[2c9x\|2c9x]]</div~~></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=MSS:(MOLYBDOPTERIN-S,S)-OXO-MOLYBDENUM'>MSS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ca3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ca3 OCA], [https://pdbe.org/2ca3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ca3 RCSB], [https://www.ebi.ac.uk/pdbsum/2ca3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ca3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ca3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ca3 OCA], [https://pdbe.org/2ca3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ca3 RCSB], [https://www.ebi.ac.uk/pdbsum/2ca3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ca3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/Q9LA16_STANO Q9LA16_STANO]] The exact function is not known. Can catalyze the reduction of a variety of substrates like dimethyl sulfoxide, trimethylamine N-oxide, phenylmethyl sulfoxide and L-methionine sulfoxide. Cannot reduce cyclic N-oxides. Shows no activity as sulfite oxidase.[SAAS:SAAS00086612] The exact function is not known. Can catalyze the reduction of a variety of substrates like dimethyl sulfoxide, trimethylamine N-oxide, phenylmethyl sulfoxide and L-methionine sulfoxide. Cannot reduce cyclic N-oxides. Shows no activity as sulfite oxidase (By similarity).		[https://www.uniprot.org/uniprot/Q9LA16_STANO Q9LA16_STANO] The exact function is not known. Can catalyze the reduction of a variety of substrates like dimethyl sulfoxide, trimethylamine N-oxide, phenylmethyl sulfoxide and L-methionine sulfoxide. Cannot reduce cyclic N-oxides. Shows no activity as sulfite oxidase.[SAAS:SAAS00086612] The exact function is not known. Can catalyze the reduction of a variety of substrates like dimethyl sulfoxide, trimethylamine N-oxide, phenylmethyl sulfoxide and L-methionine sulfoxide. Cannot reduce cyclic N-oxides. Shows no activity as sulfite oxidase (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Atcc 8093]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bailey, S]]		[[Category: Starkeya novella]]
	[[Category: Bernhardt~~, P V~~]]		[[Category: Bailey S]]
	[[Category: Enemark~~, J H~~]]		[[Category: Bernhardt PV]]
	[[Category: Feng, C]]		[[Category: Enemark JH]]
	[[Category: Honeychurch~~, M J~~]]		[[Category: Feng C]]
	[[Category: Kappler, U]]		[[Category: Honeychurch MJ]]
	[[Category: Tollin, G]]		[[Category: Kappler U]]
	~~[[Category: C-type cytochrome]]~~		[[Category: Tollin G]]
	~~[[Category: Heme]]~~
	~~[[Category: Molybdopterin]]~~
	~~[[Category: Mutant]]~~
	~~[[Category: Oxidoreductase]]~~
	~~[[Category: Sulfite oxidase~~]]

OCA at 06:51, 1 December 2021

2021-12-01T06:51:33Z

← Older revision		Revision as of 06:51, 1 December 2021
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	<StructureSection load='2ca3' size='340' side='right'caption='[[2ca3]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2ca3' size='340' side='right'caption='[[2ca3]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ca3]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_8093 Atcc 8093]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CA3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2CA3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ca3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_8093 Atcc 8093]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CA3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CA3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=MSS:(MOLYBDOPTERIN-S,S)-OXO-MOLYBDENUM'>MSS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=MSS:(MOLYBDOPTERIN-S,S)-OXO-MOLYBDENUM'>MSS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2blf\|2blf]], [[2bpb\|2bpb]], [[2c9x\|2c9x]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2blf\|2blf]], [[2bpb\|2bpb]], [[2c9x\|2c9x]]</div></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ca3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ca3 OCA], [~~http~~://pdbe.org/2ca3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ca3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ca3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ca3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ca3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ca3 OCA], [https://pdbe.org/2ca3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ca3 RCSB], [https://www.ebi.ac.uk/pdbsum/2ca3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ca3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/Q9LA16_STANO Q9LA16_STANO]] The exact function is not known. Can catalyze the reduction of a variety of substrates like dimethyl sulfoxide, trimethylamine N-oxide, phenylmethyl sulfoxide and L-methionine sulfoxide. Cannot reduce cyclic N-oxides. Shows no activity as sulfite oxidase.[SAAS:SAAS00086612] The exact function is not known. Can catalyze the reduction of a variety of substrates like dimethyl sulfoxide, trimethylamine N-oxide, phenylmethyl sulfoxide and L-methionine sulfoxide. Cannot reduce cyclic N-oxides. Shows no activity as sulfite oxidase (By similarity).		[[https://www.uniprot.org/uniprot/Q9LA16_STANO Q9LA16_STANO]] The exact function is not known. Can catalyze the reduction of a variety of substrates like dimethyl sulfoxide, trimethylamine N-oxide, phenylmethyl sulfoxide and L-methionine sulfoxide. Cannot reduce cyclic N-oxides. Shows no activity as sulfite oxidase.[SAAS:SAAS00086612] The exact function is not known. Can catalyze the reduction of a variety of substrates like dimethyl sulfoxide, trimethylamine N-oxide, phenylmethyl sulfoxide and L-methionine sulfoxide. Cannot reduce cyclic N-oxides. Shows no activity as sulfite oxidase (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 11:04, 26 February 2020

2020-02-26T11:04:35Z

← Older revision		Revision as of 11:04, 26 February 2020
Line 1:		Line 1:

	==Sulfite dehydrogenase from Starkeya Novella r55m mutant==		==Sulfite dehydrogenase from Starkeya Novella r55m mutant==
	<StructureSection load='2ca3' size='340' side='right' caption='[[2ca3]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2ca3' size='340' side='right'caption='[[2ca3]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ca3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_8093 Atcc 8093]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CA3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CA3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ca3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_8093 Atcc 8093]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CA3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CA3 FirstGlance]. <br>
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 8093]]		[[Category: Atcc 8093]]
			[[Category: Large Structures]]
	[[Category: Bailey, S]]		[[Category: Bailey, S]]
	[[Category: Bernhardt, P V]]		[[Category: Bernhardt, P V]]

OCA at 08:31, 23 May 2018

2018-05-23T08:31:56Z

← Older revision		Revision as of 08:31, 23 May 2018
Line 1:		Line 1:
	==~~SULFITE DEHYDROGENASE FROM STARKEYA NOVELLA R55M MUTANT~~==
			==Sulfite dehydrogenase from Starkeya Novella r55m mutant==
	<StructureSection load='2ca3' size='340' side='right' caption='[[2ca3]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2ca3' size='340' side='right' caption='[[2ca3]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=MSS:(MOLYBDOPTERIN-S,S)-OXO-MOLYBDENUM'>MSS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=MSS:(MOLYBDOPTERIN-S,S)-OXO-MOLYBDENUM'>MSS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2blf\|2blf]], [[2bpb\|2bpb]], [[2c9x\|2c9x]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2blf\|2blf]], [[2bpb\|2bpb]], [[2c9x\|2c9x]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ca3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ca3 OCA], [http://pdbe.org/2ca3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ca3 RCSB], [http://www.ebi.ac.uk/pdbsum/2ca3 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ca3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ca3 OCA], [http://pdbe.org/2ca3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ca3 RCSB], [http://www.ebi.ac.uk/pdbsum/2ca3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ca3 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ca/2ca3_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ca/2ca3_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 09:52, 8 February 2016

2016-02-08T09:52:20Z

← Older revision		Revision as of 09:52, 8 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ca3 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:24, 10 September 2015

2015-09-10T10:24:13Z

← Older revision		Revision as of 10:24, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2ca3' size='340' side='right' caption='[[2ca3]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2ca3' size='340' side='right' caption='[[2ca3]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ca3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Starkeya_novella Starkeya novella~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CA3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CA3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ca3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_8093 Atcc 8093]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CA3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CA3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=MSS:(MOLYBDOPTERIN-S,S)-OXO-MOLYBDENUM'>MSS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=MSS:(MOLYBDOPTERIN-S,S)-OXO-MOLYBDENUM'>MSS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2blf\|2blf]], [[2bpb\|2bpb]], [[2c9x\|2c9x]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2blf\|2blf]], [[2bpb\|2bpb]], [[2c9x\|2c9x]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ca3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ca3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ca3 RCSB], [http://www.ebi.ac.uk/pdbsum/2ca3 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ca3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ca3 OCA], [http://pdbe.org/2ca3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ca3 RCSB], [http://www.ebi.ac.uk/pdbsum/2ca3 PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/Q9LA16_STANO Q9LA16_STANO]] The exact function is not known. Can catalyze the reduction of a variety of substrates like dimethyl sulfoxide, trimethylamine N-oxide, phenylmethyl sulfoxide and L-methionine sulfoxide. Cannot reduce cyclic N-oxides. Shows no activity as sulfite oxidase.[SAAS:SAAS00086612] The exact function is not known. Can catalyze the reduction of a variety of substrates like dimethyl sulfoxide, trimethylamine N-oxide, phenylmethyl sulfoxide and L-methionine sulfoxide. Cannot reduce cyclic N-oxides. Shows no activity as sulfite oxidase (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 25:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2ca3" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Starkeya novella~~]]		[[Category: Atcc 8093]]
	[[Category: Bailey, S]]		[[Category: Bailey, S]]
	[[Category: Bernhardt, P V]]		[[Category: Bernhardt, P V]]

OCA at 16:52, 15 January 2015

2015-01-15T16:52:23Z

← Older revision		Revision as of 16:52, 15 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ca3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Starkeya_novella Starkeya novella]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CA3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CA3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ca3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Starkeya_novella Starkeya novella]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CA3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CA3 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=MSS:(MOLYBDOPTERIN-S,S)-OXO-MOLYBDENUM'>MSS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=MSS:(MOLYBDOPTERIN-S,S)-OXO-MOLYBDENUM'>MSS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2blf\|2blf]], [[2bpb\|2bpb]], [[2c9x\|2c9x]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2blf\|2blf]], [[2bpb\|2bpb]], [[2c9x\|2c9x]]</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ca3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ca3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ca3 RCSB], [http://www.ebi.ac.uk/pdbsum/2ca3 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ca3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ca3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ca3 RCSB], [http://www.ebi.ac.uk/pdbsum/2ca3 PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Starkeya novella]]		[[Category: Starkeya novella]]
	[[Category: Bailey, S.]]		[[Category: Bailey, S]]
	[[Category: Bernhardt, P V.]]		[[Category: Bernhardt, P V]]
	[[Category: Enemark, J H.]]		[[Category: Enemark, J H]]
	[[Category: Feng, C.]]		[[Category: Feng, C]]
	[[Category: Honeychurch, M J.]]		[[Category: Honeychurch, M J]]
	[[Category: Kappler, U.]]		[[Category: Kappler, U]]
	[[Category: Tollin, G.]]		[[Category: Tollin, G]]
	[[Category: C-type cytochrome]]		[[Category: C-type cytochrome]]
	[[Category: Heme]]		[[Category: Heme]]

OCA at 00:20, 30 September 2014

2014-09-30T00:20:33Z

← Older revision		Revision as of 00:20, 30 September 2014
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	[[Image:~~2ca3~~.~~png~~\|~~left~~\|~~200px~~]]		==SULFITE DEHYDROGENASE FROM STARKEYA NOVELLA R55M MUTANT==
			<StructureSection load='2ca3' size='340' side='right' caption='[[2ca3]], [[Resolution\|resolution]] 2.00Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2ca3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Starkeya_novella Starkeya novella]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CA3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CA3 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=MSS:(MOLYBDOPTERIN-S,S)-OXO-MOLYBDENUM'>MSS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2blf\|2blf]], [[2bpb\|2bpb]], [[2c9x\|2c9x]]</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ca3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ca3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ca3 RCSB], [http://www.ebi.ac.uk/pdbsum/2ca3 PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ca/2ca3_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Sulfite dehydrogenases (SDHs) catalyze the oxidation and detoxification of sulfite to sulfate, a reaction critical to all forms of life. Sulfite-oxidizing enzymes contain three conserved active site amino acids (Arg-55, His-57, and Tyr-236) that are crucial for catalytic competency. Here we have studied the kinetic and structural effects of two novel and one previously reported substitution (R55M, H57A, Y236F) in these residues on SDH catalysis. Both Arg-55 and His-57 were found to have key roles in substrate binding. An R55M substitution increased Km(sulfite)(app) by 2-3 orders of magnitude, whereas His-57 was required for maintaining a high substrate affinity at low pH when the imidazole ring is fully protonated. This effect may be mediated by interactions of His-57 with Arg-55 that stabilize the position of the Arg-55 side chain or, alternatively, may reflect changes in the protonation state of sulfite. Unlike what is seen for SDHWT and SDHY236F, the catalytic turnover rates of SDH R55M and SDHH57A are relatively insensitive to pH (approximately 60 and 200 s(-1), respectively). On the structural level, striking kinetic effects appeared to correlate with disorder (in SDHH57A and SDHY236F) or absence of Arg-55 (SDHR55M), suggesting that Arg-55 and the hydrogen bonding interactions it engages in are crucial for substrate binding and catalysis. The structure of SDHR55M has sulfate bound at the active site, a fact that coincides with a significant increase in the inhibitory effect of sulfate in SDHR55M. Thus, Arg-55 also appears to be involved in enabling discrimination between the substrate and product in SDH.

	~~{{STRUCTURE_2ca3\| PDB=2ca3 \| SCENE= }}~~		Molecular basis for enzymatic sulfite oxidation: how three conserved active site residues shape enzyme activity.,Bailey S, Rapson T, Johnson-Winters K, Astashkin AV, Enemark JH, Kappler U J Biol Chem. 2009 Jan 23;284(4):2053-63. Epub 2008 Nov 12. PMID:19004819<ref>PMID:19004819</ref>

	~~===SULFITE DEHYDROGENASE FROM STARKEYA NOVELLA R55M MUTANT===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_19004819}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2ca3]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Starkeya_novella Starkeya novella]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2CA3 OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:019004819<~~/~~ref><ref group="xtra">PMID:015863498</ref~~><~~references group="xtra"~~/>
	[[Category: Starkeya novella]]		[[Category: Starkeya novella]]
	[[Category: Bailey, S.]]		[[Category: Bailey, S.]]

OCA at 12:19, 23 January 2013

2013-01-23T12:19:06Z

← Older revision		Revision as of 12:19, 23 January 2013
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	[[Image:2ca3.png\|left\|200px]]		[[Image:2ca3.png\|left\|200px]]

	~~<!--~~
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	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
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	{{STRUCTURE_2ca3\| PDB=2ca3 \| SCENE= }}		{{STRUCTURE_2ca3\| PDB=2ca3 \| SCENE= }}

	===SULFITE DEHYDROGENASE FROM STARKEYA NOVELLA R55M MUTANT===		===SULFITE DEHYDROGENASE FROM STARKEYA NOVELLA R55M MUTANT===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_19004819}}, adds the Publication Abstract to the page~~
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	{{ABSTRACT_PUBMED_19004819}}		{{ABSTRACT_PUBMED_19004819}}

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	==Reference==		==Reference==
	<ref group="xtra">PMID:~~19004819~~</ref><ref group="xtra">PMID:~~15863498~~</ref><references group="xtra"/>		<ref group="xtra">PMID:019004819</ref><ref group="xtra">PMID:015863498</ref><references group="xtra"/>
	[[Category: Starkeya novella]]		[[Category: Starkeya novella]]
	[[Category: Bailey, S.]]		[[Category: Bailey, S.]]