2cb4 - Revision history

OCA at 10:25, 6 September 2023

2023-09-06T10:25:18Z

← Older revision		Revision as of 10:25, 6 September 2023
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	<StructureSection load='2cb4' size='340' side='right'caption='[[2cb4]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2cb4' size='340' side='right'caption='[[2cb4]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cb4]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_14577 Atcc 14577~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CB4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CB4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cb4]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Lysinibacillus_sphaericus Lysinibacillus sphaericus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CB4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CB4 FirstGlance]. <br>
	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=MSE:SELENOMETHIONINE'>MSE</scene~~></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[2cb6\|2cb6]]~~</~~div~~></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cb4 OCA], [https://pdbe.org/2cb4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cb4 RCSB], [https://www.ebi.ac.uk/pdbsum/2cb4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cb4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cb4 OCA], [https://pdbe.org/2cb4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cb4 RCSB], [https://www.ebi.ac.uk/pdbsum/2cb4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cb4 ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/Q03988_LYSSH Q03988_LYSSH]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Atcc 14577]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Aktories, K~~]]		[[Category: Lysinibacillus sphaericus]]
	[[Category: ~~Carpusca, I~~]]		[[Category: Aktories K]]
	[[Category: ~~Reinert, D J~~]]		[[Category: Carpusca I]]
	[[Category: ~~Schulz, G E~~]]		[[Category: Reinert DJ]]
	[[Category: ~~Adp-ribosyltransferase]]~~		[[Category: Schulz GE]]
	~~[[Category: Toxin~~]]

OCA at 06:52, 1 December 2021

2021-12-01T06:52:11Z

← Older revision		Revision as of 06:52, 1 December 2021
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	<StructureSection load='2cb4' size='340' side='right'caption='[[2cb4]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2cb4' size='340' side='right'caption='[[2cb4]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cb4]] is a 14 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_14577 Atcc 14577]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CB4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2CB4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cb4]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_14577 Atcc 14577]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CB4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CB4 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2cb6\|2cb6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2cb6\|2cb6]]</div></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2cb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cb4 OCA], [~~http~~://pdbe.org/2cb4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2cb4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2cb4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2cb4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cb4 OCA], [https://pdbe.org/2cb4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cb4 RCSB], [https://www.ebi.ac.uk/pdbsum/2cb4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cb4 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:46, 26 February 2020

2020-02-26T10:46:02Z

← Older revision		Revision as of 10:46, 26 February 2020
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	==Crystal structure of the catalytic domain of the mosquitocidal toxin from Bacillus sphaericus, mutant E197Q==		==Crystal structure of the catalytic domain of the mosquitocidal toxin from Bacillus sphaericus, mutant E197Q==
	<StructureSection load='2cb4' size='340' side='right' caption='[[2cb4]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2cb4' size='340' side='right'caption='[[2cb4]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cb4]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_14577 Atcc 14577]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CB4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CB4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cb4]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_14577 Atcc 14577]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CB4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CB4 FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Atcc 14577]]		[[Category: Atcc 14577]]
			[[Category: Large Structures]]
	[[Category: Aktories, K]]		[[Category: Aktories, K]]
	[[Category: Carpusca, I]]		[[Category: Carpusca, I]]

OCA at 08:01, 23 May 2018

2018-05-23T08:01:14Z

← Older revision		Revision as of 08:01, 23 May 2018
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	==~~CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF THE MOSQUITOCIDAL TOXIN FROM BACILLUS SPHAERICUS~~, ~~MUTANT~~ E197Q==
			==Crystal structure of the catalytic domain of the mosquitocidal toxin from Bacillus sphaericus, mutant E197Q==
	<StructureSection load='2cb4' size='340' side='right' caption='[[2cb4]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2cb4' size='340' side='right' caption='[[2cb4]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
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	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2cb6\|2cb6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2cb6\|2cb6]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cb4 OCA], [http://pdbe.org/2cb4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cb4 RCSB], [http://www.ebi.ac.uk/pdbsum/2cb4 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cb4 OCA], [http://pdbe.org/2cb4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cb4 RCSB], [http://www.ebi.ac.uk/pdbsum/2cb4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2cb4 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:49, 11 September 2015

2015-09-11T22:49:27Z

← Older revision		Revision as of 22:49, 11 September 2015
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	<StructureSection load='2cb4' size='340' side='right' caption='[[2cb4]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2cb4' size='340' side='right' caption='[[2cb4]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cb4]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Lysinibacillus_sphaericus Lysinibacillus sphaericus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CB4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CB4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cb4]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_14577 Atcc 14577]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CB4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CB4 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2cb6\|2cb6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2cb6\|2cb6]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cb4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cb4 RCSB], [http://www.ebi.ac.uk/pdbsum/2cb4 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cb4 OCA], [http://pdbe.org/2cb4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cb4 RCSB], [http://www.ebi.ac.uk/pdbsum/2cb4 PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2cb4" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Lysinibacillus sphaericus~~]]		[[Category: Atcc 14577]]
	[[Category: Aktories, K]]		[[Category: Aktories, K]]
	[[Category: Carpusca, I]]		[[Category: Carpusca, I]]

OCA at 17:29, 15 January 2015

2015-01-15T17:29:24Z

← Older revision		Revision as of 17:29, 15 January 2015
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	</StructureSection>		</StructureSection>
	[[Category: Lysinibacillus sphaericus]]		[[Category: Lysinibacillus sphaericus]]
	[[Category: Aktories, K.]]		[[Category: Aktories, K]]
	[[Category: Carpusca, I.]]		[[Category: Carpusca, I]]
	[[Category: Reinert, D J.]]		[[Category: Reinert, D J]]
	[[Category: Schulz, G E.]]		[[Category: Schulz, G E]]
	[[Category: Adp-ribosyltransferase]]		[[Category: Adp-ribosyltransferase]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 08:17, 8 October 2014

2014-10-08T08:17:57Z

← Older revision		Revision as of 08:17, 8 October 2014
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	[[~~Image~~:~~2cb4~~.~~png~~\|~~left~~\|~~200px~~]]		==CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF THE MOSQUITOCIDAL TOXIN FROM BACILLUS SPHAERICUS, MUTANT E197Q==
			<StructureSection load='2cb4' size='340' side='right' caption='[[2cb4]], [[Resolution\|resolution]] 2.50Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2cb4]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Lysinibacillus_sphaericus Lysinibacillus sphaericus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CB4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CB4 FirstGlance]. <br>
			</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2cb6\|2cb6]]</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cb4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cb4 RCSB], [http://www.ebi.ac.uk/pdbsum/2cb4 PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The catalytic domain of a mosquitocidal toxin prolonged by a C-terminal 44 residue linker connecting to four ricin B-like domains was crystallized. Three crystal structures were established at resolutions between 2.5A and 3.0A using multi-wavelength and single-wavelength anomalous X-ray diffraction as well as molecular replacement phasing techniques. The chainfold of the toxin fragment corresponds to those of ADP-ribosylating enzymes. At pH 4.3 the fragment is associated in a C(7)-symmetric heptamer in agreement with an aggregate of similar size observed by size-exclusion chromatography. In two distinct crystal forms, the heptamers formed nearly spherical, D(7)-symmetric tetradecamers. Another crystal form obtained at pH 6.3 contained a recurring C(2)-symmetric tetramer, which, however, was not stable in solution. On the basis of the common chainfold and NAD(+)-binding site of all ADP-ribosyl transferases, the NAD(+)-binding site of the toxin was assigned at a high confidence level. In all three crystal forms the NAD(+) site was occupied by part of the 44 residue linker, explaining the known inhibitory effect of this polypeptide region. The structure showed that the cleavage site for toxin activation is in a highly mobile loop that is exposed in the monomer. Since it contains the inhibitory linker as a crucial part of the association contact, the observed heptamer is inactive. Moreover, the heptamer cannot be activated by proteolysis because the activation loop is at the ring center and not accessible for proteases. Therefore the heptamer, or possibly the tetradecamer, seems to represent an inactive storage form of the toxin.

	~~{{STRUCTURE_2cb4\| PDB=2cb4 \| SCENE= }}~~		Structure of the mosquitocidal toxin from Bacillus sphaericus.,Reinert DJ, Carpusca I, Aktories K, Schulz GE J Mol Biol. 2006 Apr 7;357(4):1226-36. Epub 2006 Jan 27. PMID:16483607<ref>PMID:16483607</ref>

	~~===CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF THE MOSQUITOCIDAL TOXIN FROM BACILLUS SPHAERICUS~~, ~~MUTANT E197Q===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_16483607}}~~		== References ==
			<references/>
	==~~About this Structure~~==		__TOC__
	~~[[2cb4]] is a 14 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Lysinibacillus_sphaericus Lysinibacillus sphaericus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CB4 OCA].~~		</StructureSection>
	[[Category: Lysinibacillus sphaericus]]		[[Category: Lysinibacillus sphaericus]]
	[[Category: Aktories, K.]]		[[Category: Aktories, K.]]

OCA: Protected "2cb4" [edit=sysop:move=sysop]

2013-01-06T11:35:36Z

Protected "2cb4" [edit=sysop:move=sysop]

← Older revision	Revision as of 11:35, 6 January 2013
(No difference)

OCA at 11:35, 6 January 2013

2013-01-06T11:35:34Z

← Older revision		Revision as of 11:35, 6 January 2013
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	~~{{Seed}}~~
	[[Image:2cb4.png\|left\|200px]]		[[Image:2cb4.png\|left\|200px]]

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	{{STRUCTURE_2cb4\| PDB=2cb4 \| SCENE= }}		{{STRUCTURE_2cb4\| PDB=2cb4 \| SCENE= }}

	===CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF THE MOSQUITOCIDAL TOXIN FROM BACILLUS SPHAERICUS, MUTANT E197Q===		===CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF THE MOSQUITOCIDAL TOXIN FROM BACILLUS SPHAERICUS, MUTANT E197Q===


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	{{ABSTRACT_PUBMED_16483607}}		{{ABSTRACT_PUBMED_16483607}}

	==About this Structure==		==About this Structure==
	~~2CB4 is a~~ [[~~Single protein~~]] structure of sequence from [http://en.wikipedia.org/wiki/Lysinibacillus_sphaericus Lysinibacillus sphaericus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CB4 OCA].		[[2cb4]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Lysinibacillus_sphaericus Lysinibacillus sphaericus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CB4 OCA].

	~~==Reference==~~
	Structure of the mosquitocidal toxin from Bacillus sphaericus., Reinert DJ, Carpusca I, Aktories K, Schulz GE, J Mol Biol. 2006 Apr 7;357(4):1226-36. Epub 2006 Jan 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16483607 16483607]
	[[Category: Lysinibacillus sphaericus]]		[[Category: Lysinibacillus sphaericus]]
	~~[[Category: Single protein]]~~
	[[Category: Aktories, K.]]		[[Category: Aktories, K.]]
	[[Category: Carpusca, I.]]		[[Category: Carpusca, I.]]
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	[[Category: Adp-ribosyltransferase]]		[[Category: Adp-ribosyltransferase]]
	[[Category: Toxin]]		[[Category: Toxin]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:59:08 2008''~~

OCA at 09:59, 29 July 2008

2008-07-29T09:59:11Z

← Older revision		Revision as of 09:59, 29 July 2008
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	[[Image:2cb4.~~gif~~\|left\|200px]]		{{Seed}}
			[[Image:2cb4.png\|left\|200px]]

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	{{STRUCTURE_2cb4\| PDB=2cb4 \| SCENE= }}		{{STRUCTURE_2cb4\| PDB=2cb4 \| SCENE= }}

	~~'''~~CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF THE MOSQUITOCIDAL TOXIN FROM BACILLUS SPHAERICUS, MUTANT E197Q~~'''~~		===CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF THE MOSQUITOCIDAL TOXIN FROM BACILLUS SPHAERICUS, MUTANT E197Q===


	~~==Overview==~~		<!--
	~~The catalytic domain of a mosquitocidal toxin prolonged by a C~~-~~terminal 44 residue linker connecting to four ricin B~~-like domains was crystallized. Three crystal structures were established at resolutions between 2.5A and 3.0A using multi-wavelength and single-wavelength anomalous X-ray diffraction as well as molecular replacement phasing techniques. The ~~chainfold of~~ the ~~toxin fragment corresponds~~ to ~~those of ADP-ribosylating enzymes. At pH 4.3~~ the ~~fragment is associated in a C~~(7)-symmetric heptamer in agreement with an aggregate of similar size observed by size-exclusion chromatography. In two distinct crystal forms, the heptamers formed nearly spherical, D(7)-symmetric tetradecamers. Another crystal form obtained at ~~pH 6~~.3 contained a recurring C(2)-symmetric tetramer, which, however, was not stable in solution. On the basis of the common chainfold and NAD(+)-binding site of all ADP-ribosyl transferases, the NAD(+)-binding site of the toxin was assigned at a high confidence level. ~~In all three crystal forms the NAD(+~~) ~~site was occupied by part of the 44 residue linker~~, ~~explaining the known inhibitory effect of this polypeptide region. The structure showed that the cleavage site for toxin activation~~ is ~~in a highly mobile loop that is exposed in~~ the monomer. Since it contains the inhibitory linker as a crucial part of the association contact, the observed heptamer is inactive. Moreover, the heptamer cannot be activated by proteolysis because the activation loop is at the ring center and not accessible for proteases. Therefore the heptamer, or possibly the tetradecamer, seems to represent an inactive storage form of the toxin.		The line below this paragraph, {{ABSTRACT_PUBMED_16483607}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 16483607 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_16483607}}

	==About this Structure==		==About this Structure==
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	[[Category: Adp-ribosyltransferase]]		[[Category: Adp-ribosyltransferase]]
	[[Category: Toxin]]		[[Category: Toxin]]
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