2cbf - Revision history

OCA at 09:17, 14 February 2024

2024-02-14T09:17:50Z

← Older revision		Revision as of 09:17, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='2cbf' size='340' side='right'caption='[[2cbf]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='2cbf' size='340' side='right'caption='[[2cbf]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cbf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_14581 Atcc 14581~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CBF FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cbf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Priestia_megaterium Priestia megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CBF FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SAH:S~~-~~ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.1Å</td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>CBIF ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=1404 ATCC 14581])</td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
	~~<tr id='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/Precorrin~~-~~4_C(11)~~-~~methyltransferase Precorrin~~-~~4 C(11)-methyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.133 2.1.1.133]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cbf OCA], [https://pdbe.org/2cbf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cbf RCSB], [https://www.ebi.ac.uk/pdbsum/2cbf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cbf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cbf OCA], [https://pdbe.org/2cbf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cbf RCSB], [https://www.ebi.ac.uk/pdbsum/2cbf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cbf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/~~CBIF_BACME CBIF_BACME]~~] Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5A.		[https://www.uniprot.org/uniprot/CBIF_PRIMG CBIF_PRIMG] Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5A.<ref>PMID:23922391</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 21:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cbf ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cbf ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Biosynthesis of the corrin ring of vitamin B12 requires the action of six S-adenosyl-L-methionine (AdoMet) dependent transmethylases, closely related in sequence. The first X-ray structure of one of these, cobalt-precorrin-4 transmethylase, CbiF, from Bacillus megaterium has been determined to a resolution of 2.4 A. CbiF contains two alphabeta domains forming a trough in which S-adenosyl-L-homocysteine (AdoHcy) binds. The location of AdoHcy and a number of conserved residues, helps define the precorrin binding site. A second crystal form determined at 3.1 A resolution highlights the flexibility of two loops around this site. CbiF employs a unique mode of AdoHcy binding and represents a new class of transmethylase.

	The X-ray structure of a cobalamin biosynthetic enzyme, cobalt-precorrin-4 methyltransferase.,Schubert HL, Wilson KS, Raux E, Woodcock SC, Warren MJ Nat Struct Biol. 1998 Jul;5(7):585-92. PMID:9665173<ref>PMID:9665173</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2cbf" style="background-color:#fffaf0;"></div>~~
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Atcc 14581]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Raux, E~~]]		[[Category: Priestia megaterium]]
	[[Category: ~~Schubert, H L~~]]		[[Category: Raux E]]
	[[Category: ~~Warren, M J~~]]		[[Category: Schubert HL]]
	[[Category: ~~Wilson, K S~~]]		[[Category: Warren MJ]]
	[[Category: ~~Woodcock, S C~~]]		[[Category: Wilson KS]]
	[[Category: ~~Cobalamin biosynthesis]]~~		[[Category: Woodcock SC]]
	~~[[Category: Methylase]]~~
	~~[[Category: Methyltransferase]]~~
	~~[[Category: Precorrin-4 methyltransferase~~]]

OCA at 11:43, 3 February 2021

2021-02-03T11:43:24Z

← Older revision		Revision as of 11:43, 3 February 2021
Line 1:		Line 1:

	==THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF, FROM BACILLUS MEGATERIUM, WITH THE HIS-TAG CLEAVED OFF==		==THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF, FROM BACILLUS MEGATERIUM, WITH THE HIS-TAG CLEAVED OFF==
	<StructureSection load='2cbf' size='340' side='right' caption='[[2cbf]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='2cbf' size='340' side='right'caption='[[2cbf]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cbf]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_14581 Atcc 14581]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CBF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2CBF FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cbf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_14581 Atcc 14581]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CBF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CBIF ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1404 ATCC 14581])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CBIF ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1404 ATCC 14581])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Precorrin-4_C(11)-methyltransferase Precorrin-4 C(11)-methyltransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.133 2.1.1.133] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Precorrin-4_C(11)-methyltransferase Precorrin-4 C(11)-methyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.133 2.1.1.133] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cbf OCA], [~~http~~://pdbe.org/2cbf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2cbf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2cbf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2cbf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cbf OCA], [https://pdbe.org/2cbf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cbf RCSB], [https://www.ebi.ac.uk/pdbsum/2cbf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cbf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/CBIF_BACME CBIF_BACME]] Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5A.		[[https://www.uniprot.org/uniprot/CBIF_BACME CBIF_BACME]] Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5A.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 14581]]		[[Category: Atcc 14581]]
			[[Category: Large Structures]]
	[[Category: Raux, E]]		[[Category: Raux, E]]
	[[Category: Schubert, H L]]		[[Category: Schubert, H L]]

OCA at 08:41, 23 May 2018

2018-05-23T08:41:35Z

← Older revision		Revision as of 08:41, 23 May 2018
Line 1:		Line 1:

	==THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF, FROM BACILLUS MEGATERIUM, WITH THE HIS-TAG CLEAVED OFF==		==THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF, FROM BACILLUS MEGATERIUM, WITH THE HIS-TAG CLEAVED OFF==
	<StructureSection load='2cbf' size='340' side='right' caption='[[2cbf]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='2cbf' size='340' side='right' caption='[[2cbf]], [[Resolution\|resolution]] 3.10Å' scene=''>
Line 6:		Line 7:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CBIF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1404 ATCC 14581])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CBIF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1404 ATCC 14581])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Precorrin-4_C(11)-methyltransferase Precorrin-4 C(11)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.133 2.1.1.133] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Precorrin-4_C(11)-methyltransferase Precorrin-4 C(11)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.133 2.1.1.133] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cbf OCA], [http://pdbe.org/2cbf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cbf RCSB], [http://www.ebi.ac.uk/pdbsum/2cbf PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cbf OCA], [http://pdbe.org/2cbf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cbf RCSB], [http://www.ebi.ac.uk/pdbsum/2cbf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2cbf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cb/2cbf_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cb/2cbf_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cbf ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 01:03, 12 September 2015

2015-09-12T01:03:45Z

← Older revision		Revision as of 01:03, 12 September 2015
Line 2:		Line 2:
	<StructureSection load='2cbf' size='340' side='right' caption='[[2cbf]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='2cbf' size='340' side='right' caption='[[2cbf]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cbf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_megaterium Bacillus megaterium~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CBF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CBF FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cbf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_14581 Atcc 14581]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CBF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CBF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CBIF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1404 ~~Bacillus megaterium~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CBIF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1404 ATCC 14581])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Precorrin-4_C(11)-methyltransferase Precorrin-4 C(11)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.133 2.1.1.133] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Precorrin-4_C(11)-methyltransferase Precorrin-4 C(11)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.133 2.1.1.133] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cbf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cbf RCSB], [http://www.ebi.ac.uk/pdbsum/2cbf PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cbf OCA], [http://pdbe.org/2cbf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cbf RCSB], [http://www.ebi.ac.uk/pdbsum/2cbf PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2cbf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bacillus megaterium~~]]		[[Category: Atcc 14581]]
	[[Category: Raux, E]]		[[Category: Raux, E]]
	[[Category: Schubert, H L]]		[[Category: Schubert, H L]]

OCA at 21:46, 25 December 2014

2014-12-25T21:46:56Z

← Older revision		Revision as of 21:46, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cbf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CBF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CBF FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cbf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CBF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CBF FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CBIF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1404 Bacillus megaterium])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CBIF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1404 Bacillus megaterium])</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Precorrin-4_C(11)-methyltransferase Precorrin-4 C(11)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.133 2.1.1.133] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Precorrin-4_C(11)-methyltransferase Precorrin-4 C(11)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.133 2.1.1.133] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cbf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cbf RCSB], [http://www.ebi.ac.uk/pdbsum/2cbf PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cbf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cbf RCSB], [http://www.ebi.ac.uk/pdbsum/2cbf PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/CBIF_BACME CBIF_BACME]] Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5A.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus megaterium]]		[[Category: Bacillus megaterium]]
	[[Category: Raux, E.]]		[[Category: Raux, E]]
	[[Category: Schubert, H L.]]		[[Category: Schubert, H L]]
	[[Category: Warren, M J.]]		[[Category: Warren, M J]]
	[[Category: Wilson, K S.]]		[[Category: Wilson, K S]]
	[[Category: Woodcock, S C.]]		[[Category: Woodcock, S C]]
	[[Category: Cobalamin biosynthesis]]		[[Category: Cobalamin biosynthesis]]
	[[Category: Methylase]]		[[Category: Methylase]]
	[[Category: Methyltransferase]]		[[Category: Methyltransferase]]
	[[Category: Precorrin-4 methyltransferase]]		[[Category: Precorrin-4 methyltransferase]]

OCA at 01:21, 30 September 2014

2014-09-30T01:21:23Z

← Older revision		Revision as of 01:21, 30 September 2014
Line 1:		Line 1:
	[[Image:~~2cbf~~.~~png~~\|~~left~~\|~~200px~~]]		==THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF, FROM BACILLUS MEGATERIUM, WITH THE HIS-TAG CLEAVED OFF==
			<StructureSection load='2cbf' size='340' side='right' caption='[[2cbf]], [[Resolution\|resolution]] 3.10Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2cbf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CBF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CBF FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene><br>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CBIF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1404 Bacillus megaterium])</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Precorrin-4_C(11)-methyltransferase Precorrin-4 C(11)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.133 2.1.1.133] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cbf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cbf RCSB], [http://www.ebi.ac.uk/pdbsum/2cbf PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cb/2cbf_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Biosynthesis of the corrin ring of vitamin B12 requires the action of six S-adenosyl-L-methionine (AdoMet) dependent transmethylases, closely related in sequence. The first X-ray structure of one of these, cobalt-precorrin-4 transmethylase, CbiF, from Bacillus megaterium has been determined to a resolution of 2.4 A. CbiF contains two alphabeta domains forming a trough in which S-adenosyl-L-homocysteine (AdoHcy) binds. The location of AdoHcy and a number of conserved residues, helps define the precorrin binding site. A second crystal form determined at 3.1 A resolution highlights the flexibility of two loops around this site. CbiF employs a unique mode of AdoHcy binding and represents a new class of transmethylase.

	~~{{STRUCTURE_2cbf\| PDB=2cbf \| SCENE= }}~~		The X-ray structure of a cobalamin biosynthetic enzyme, cobalt-precorrin-4 methyltransferase.,Schubert HL, Wilson KS, Raux E, Woodcock SC, Warren MJ Nat Struct Biol. 1998 Jul;5(7):585-92. PMID:9665173<ref>PMID:9665173</ref>

	~~===THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF, FROM BACILLUS MEGATERIUM~~, ~~WITH THE HIS-TAG CLEAVED OFF===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_9665173}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2cbf]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_megaterium Bacillus megaterium]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2CBF OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:009665173<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Bacillus megaterium]]		[[Category: Bacillus megaterium]]
	[[Category: Raux, E.]]		[[Category: Raux, E.]]

OCA: Protected "2cbf" [edit=sysop:move=sysop]

2013-01-06T10:36:20Z

Protected "2cbf" [edit=sysop:move=sysop]

← Older revision	Revision as of 10:36, 6 January 2013
(No difference)

OCA at 10:36, 6 January 2013

2013-01-06T10:36:16Z

← Older revision		Revision as of 10:36, 6 January 2013
Line 1:		Line 1:
	~~{{Seed}}~~
	[[Image:2cbf.png\|left\|200px]]		[[Image:2cbf.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2cbf", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2cbf\| PDB=2cbf \| SCENE= }}		{{STRUCTURE_2cbf\| PDB=2cbf \| SCENE= }}

	===THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF, FROM BACILLUS MEGATERIUM, WITH THE HIS-TAG CLEAVED OFF===		===THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF, FROM BACILLUS MEGATERIUM, WITH THE HIS-TAG CLEAVED OFF===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_9665173}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 9665173 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_9665173}}		{{ABSTRACT_PUBMED_9665173}}

	==About this Structure==		==About this Structure==
	~~2CBF~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CBF OCA].		[[2cbf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CBF OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~9665173~~</ref><references group="xtra"/>		<ref group="xtra">PMID:009665173</ref><references group="xtra"/>
	[[Category: Bacillus megaterium]]		[[Category: Bacillus megaterium]]
	[[Category: Raux, E.]]		[[Category: Raux, E.]]
Line 32:		Line 20:
	[[Category: Cobalamin biosynthesis]]		[[Category: Cobalamin biosynthesis]]
	[[Category: Methylase]]		[[Category: Methylase]]
			[[Category: Methyltransferase]]
	[[Category: Precorrin-4 methyltransferase]]		[[Category: Precorrin-4 methyltransferase]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 08:51:44 2009''~~

OCA at 06:51, 18 February 2009

2009-02-18T06:51:45Z

← Older revision		Revision as of 06:51, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
	2CBF is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CBF OCA].		2CBF is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CBF OCA].

	==Reference==		==Reference==
	~~The X-ray structure of a cobalamin biosynthetic enzyme, cobalt-precorrin-4 methyltransferase., Schubert HL, Wilson KS, Raux E, Woodcock SC, Warren MJ, Nat Struct Biol. 1998 Jul;5(7):585-92.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/9665173 9665173]~~		<ref group="xtra">PMID:9665173</ref><references group="xtra"/>
	[[Category: Bacillus megaterium]]		[[Category: Bacillus megaterium]]
	~~[[Category: Single protein]]~~
	[[Category: Raux, E.]]		[[Category: Raux, E.]]
	[[Category: Schubert, H L.]]		[[Category: Schubert, H L.]]
Line 35:		Line 34:
	[[Category: Precorrin-4 methyltransferase]]		[[Category: Precorrin-4 methyltransferase]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 15~~:03:~~45 2008~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 08:51:44 2009''

OCA at 12:03, 29 July 2008

2008-07-29T12:03:48Z

← Older revision		Revision as of 12:03, 29 July 2008
Line 1:		Line 1:
	[[Image:2cbf.~~gif~~\|left\|200px]]		{{Seed}}
			[[Image:2cbf.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_2cbf\| PDB=2cbf \| SCENE= }}		{{STRUCTURE_2cbf\| PDB=2cbf \| SCENE= }}

	~~'''~~THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF, FROM BACILLUS MEGATERIUM, WITH THE HIS-TAG CLEAVED OFF~~'''~~		===THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF, FROM BACILLUS MEGATERIUM, WITH THE HIS-TAG CLEAVED OFF===


	~~==Overview==~~		<!--
	~~Biosynthesis of the corrin ring of vitamin B12 requires the action of six S~~-~~adenosyl~~-~~L-methionine (AdoMet) dependent transmethylases, closely related in sequence.~~ The ~~first X-ray structure of one of these~~, ~~cobalt-precorrin-4 transmethylase~~, ~~CbiF, from Bacillus megaterium has been determined~~ to ~~a resolution of 2~~.~~4 A~~. ~~CbiF contains two alphabeta domains forming a trough in which S-adenosyl-L-homocysteine (AdoHcy~~) ~~binds. The location of AdoHcy and a number of conserved residues~~, ~~helps define~~ the precorrin binding site. A second crystal form determined at 3.1 A resolution highlights the flexibility of two loops around this site. CbiF employs a unique mode of AdoHcy binding and represents a new class of transmethylase.		The line below this paragraph, {{ABSTRACT_PUBMED_9665173}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 9665173 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_9665173}}

	==About this Structure==		==About this Structure==
Line 30:		Line 34:
	[[Category: Methylase]]		[[Category: Methylase]]
	[[Category: Precorrin-4 methyltransferase]]		[[Category: Precorrin-4 methyltransferase]]
	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 21~~:39:05 2008''
			''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:03:45 2008''