2cd9 - Revision history

OCA at 00:50, 21 November 2024

2024-11-21T00:50:18Z

← Older revision		Revision as of 00:50, 21 November 2024
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	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cd/2cd9_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cd/2cd9_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/~~initialview01~~.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cd9 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cd9 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The hyperthermophilic archaeon Sulfolobus solfataricus grows optimally above 80 degrees C and utilizes an unusual, promiscuous, non-phosphorylative Entner-Doudoroff pathway to metabolize both glucose and galactose. The first enzyme in this pathway, glucose dehydrogenase, catalyzes the oxidation of glucose to gluconate, but has been shown to have activity with a broad range of sugar substrates, including glucose, galactose, xylose, and L-arabinose, with a requirement for the glucose stereo configuration at the C2 and C3 positions. Here we report the crystal structure of the apo form of glucose dehydrogenase to a resolution of 1.8 A and a complex with its required cofactor, NADP+, to a resolution of 2.3 A. A T41A mutation was engineered to enable the trapping of substrate in the crystal. Complexes of the enzyme with D-glucose and D-xylose are presented to resolutions of 1.6 and 1.5 A, respectively, that provide evidence of selectivity for the beta-anomeric, pyranose form of the substrate, and indicate that this is the productive substrate form. The nature of the promiscuity of glucose dehydrogenase is also elucidated, and a physiological role for this enzyme in xylose metabolism is suggested. Finally, the structure suggests that the mechanism of sugar oxidation by this enzyme may be similar to that described for human sorbitol dehydrogenase.

			The structural basis of substrate promiscuity in glucose dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus.,Milburn CC, Lamble HJ, Theodossis A, Bull SD, Hough DW, Danson MJ, Taylor GL J Biol Chem. 2006 May 26;281(21):14796-804. Epub 2006 Mar 23. PMID:16556607<ref>PMID:16556607</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 2cd9" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:38, 1 May 2024

2024-05-01T06:38:46Z

← Older revision		Revision as of 06:38, 1 May 2024
Line 3:		Line 3:
	<StructureSection load='2cd9' size='340' side='right'caption='[[2cd9]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2cd9' size='340' side='right'caption='[[2cd9]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cd9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~'saccharolobus_solfataricus' 'saccharolobus~~ solfataricus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CD9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CD9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cd9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CD9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CD9 FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[2cda\|2cda]], [[2cdb\|2cdb]], [[2cdc\|2cdc]]</div></td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Glucose_1-dehydrogenase Glucose 1-dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.47 1.1.1.47]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cd9 OCA], [https://pdbe.org/2cd9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cd9 RCSB], [https://www.ebi.ac.uk/pdbsum/2cd9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cd9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cd9 OCA], [https://pdbe.org/2cd9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cd9 RCSB], [https://www.ebi.ac.uk/pdbsum/2cd9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cd9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/~~GLCDH_SULSF GLCDH_SULSF]~~] Catalyzes the NAD(P)(+)-dependent oxidation of D-glucose to D-gluconate via gluconolactone. Displays broad substrate specificity since it is able to catalyze the oxidation of a number of alternative aldose sugars, such as D-galactose, D-xylose and L-arabinose, to the corresponding glyconate. Can utilize both NAD(+) and NADP(+) as electron acceptor. Physiologically, seems to be involved in the degradation of both glucose and galactose through a non-phosphorylative variant of the Entner-Doudoroff pathway.<ref>PMID:12824170</ref> <ref>PMID:3827812</ref>		[https://www.uniprot.org/uniprot/GLCDH_SACSO GLCDH_SACSO] Catalyzes the NAD(P)(+)-dependent oxidation of D-glucose to D-gluconate via gluconolactone. Displays broad substrate specificity since it is able to catalyze the oxidation of a number of alternative aldose sugars, such as D-galactose, D-xylose and L-arabinose, to the corresponding glyconate. Can utilize both NAD(+) and NADP(+) as electron acceptor. Physiologically, seems to be involved in the degradation of both glucose and galactose through a non-phosphorylative variant of the Entner-Doudoroff pathway.<ref>PMID:12824170</ref> <ref>PMID:3827812</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 21:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cd9 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cd9 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The hyperthermophilic archaeon Sulfolobus solfataricus grows optimally above 80 degrees C and utilizes an unusual, promiscuous, non-phosphorylative Entner-Doudoroff pathway to metabolize both glucose and galactose. The first enzyme in this pathway, glucose dehydrogenase, catalyzes the oxidation of glucose to gluconate, but has been shown to have activity with a broad range of sugar substrates, including glucose, galactose, xylose, and L-arabinose, with a requirement for the glucose stereo configuration at the C2 and C3 positions. Here we report the crystal structure of the apo form of glucose dehydrogenase to a resolution of 1.8 A and a complex with its required cofactor, NADP+, to a resolution of 2.3 A. A T41A mutation was engineered to enable the trapping of substrate in the crystal. Complexes of the enzyme with D-glucose and D-xylose are presented to resolutions of 1.6 and 1.5 A, respectively, that provide evidence of selectivity for the beta-anomeric, pyranose form of the substrate, and indicate that this is the productive substrate form. The nature of the promiscuity of glucose dehydrogenase is also elucidated, and a physiological role for this enzyme in xylose metabolism is suggested. Finally, the structure suggests that the mechanism of sugar oxidation by this enzyme may be similar to that described for human sorbitol dehydrogenase.

	The structural basis of substrate promiscuity in glucose dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus.,Milburn CC, Lamble HJ, Theodossis A, Bull SD, Hough DW, Danson MJ, Taylor GL J Biol Chem. 2006 May 26;281(21):14796-804. Epub 2006 Mar 23. PMID:16556607<ref>PMID:16556607</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2cd9" style="background-color:#fffaf0;"></div>~~
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Saccharolobus solfataricus]]		[[Category: Saccharolobus solfataricus]]
	~~[[Category: Glucose 1-dehydrogenase]]~~		[[Category: Danson MJ]]
	~~[[Category: Large Structures]]~~		[[Category: Hough DW]]
	[[Category: Danson~~, M J~~]]		[[Category: Lamble HJ]]
	[[Category: Hough~~, D W~~]]		[[Category: Milburn CC]]
	[[Category: Lamble~~, H J~~]]		[[Category: Taylor GL]]
	[[Category: Milburn~~, C C~~]]		[[Category: Theodossis A]]
	[[Category: Taylor~~, G L~~]]
	[[Category: Theodossis, A]]
	~~[[Category: Glucose dehydrogenase]]~~
	~~[[Category: Medium chain dehydrogenase family]]~~
	~~[[Category: Oxidoreductase~~]]

OCA at 06:53, 1 December 2021

2021-12-01T06:53:21Z

← Older revision		Revision as of 06:53, 1 December 2021
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	<StructureSection load='2cd9' size='340' side='right'caption='[[2cd9]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2cd9' size='340' side='right'caption='[[2cd9]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cd9]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/'saccharolobus_solfataricus' 'saccharolobus solfataricus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CD9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2CD9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cd9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/'saccharolobus_solfataricus' 'saccharolobus solfataricus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CD9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CD9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2cda\|2cda]], [[2cdb\|2cdb]], [[2cdc\|2cdc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2cda\|2cda]], [[2cdb\|2cdb]], [[2cdc\|2cdc]]</div></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Glucose_1-dehydrogenase Glucose 1-dehydrogenase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.47 1.1.1.47] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glucose_1-dehydrogenase Glucose 1-dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.47 1.1.1.47] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2cd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cd9 OCA], [~~http~~://pdbe.org/2cd9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2cd9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2cd9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2cd9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cd9 OCA], [https://pdbe.org/2cd9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cd9 RCSB], [https://www.ebi.ac.uk/pdbsum/2cd9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cd9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/GLCDH_SULSF GLCDH_SULSF]] Catalyzes the NAD(P)(+)-dependent oxidation of D-glucose to D-gluconate via gluconolactone. Displays broad substrate specificity since it is able to catalyze the oxidation of a number of alternative aldose sugars, such as D-galactose, D-xylose and L-arabinose, to the corresponding glyconate. Can utilize both NAD(+) and NADP(+) as electron acceptor. Physiologically, seems to be involved in the degradation of both glucose and galactose through a non-phosphorylative variant of the Entner-Doudoroff pathway.<ref>PMID:12824170</ref> <ref>PMID:3827812</ref>		[[https://www.uniprot.org/uniprot/GLCDH_SULSF GLCDH_SULSF]] Catalyzes the NAD(P)(+)-dependent oxidation of D-glucose to D-gluconate via gluconolactone. Displays broad substrate specificity since it is able to catalyze the oxidation of a number of alternative aldose sugars, such as D-galactose, D-xylose and L-arabinose, to the corresponding glyconate. Can utilize both NAD(+) and NADP(+) as electron acceptor. Physiologically, seems to be involved in the degradation of both glucose and galactose through a non-phosphorylative variant of the Entner-Doudoroff pathway.<ref>PMID:12824170</ref> <ref>PMID:3827812</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 11:12, 26 February 2020

2020-02-26T11:12:05Z

← Older revision		Revision as of 11:12, 26 February 2020
Line 1:		Line 1:

	==Sulfolobus solfataricus Glucose Dehydrogenase 1 - apo form==		==Sulfolobus solfataricus Glucose Dehydrogenase 1 - apo form==
	<StructureSection load='2cd9' size='340' side='right' caption='[[2cd9]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2cd9' size='340' side='right'caption='[[2cd9]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cd9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/'saccharolobus_solfataricus' 'saccharolobus solfataricus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CD9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CD9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cd9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/'saccharolobus_solfataricus' 'saccharolobus solfataricus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CD9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CD9 FirstGlance]. <br>
Line 36:		Line 36:
	[[Category: Saccharolobus solfataricus]]		[[Category: Saccharolobus solfataricus]]
	[[Category: Glucose 1-dehydrogenase]]		[[Category: Glucose 1-dehydrogenase]]
			[[Category: Large Structures]]
	[[Category: Danson, M J]]		[[Category: Danson, M J]]
	[[Category: Hough, D W]]		[[Category: Hough, D W]]

OCA at 08:43, 23 May 2018

2018-05-23T08:43:05Z

← Older revision		Revision as of 08:43, 23 May 2018
Line 1:		Line 1:
	==~~SULFOLOBUS SOLFATARICUS GLUCOSE DEHYDROGENASE~~ 1 - ~~APO FORM~~==
			==Sulfolobus solfataricus Glucose Dehydrogenase 1 - apo form==
	<StructureSection load='2cd9' size='340' side='right' caption='[[2cd9]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2cd9' size='340' side='right' caption='[[2cd9]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cd9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Atcc_35091 Atcc 35091~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CD9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CD9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cd9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/'saccharolobus_solfataricus' 'saccharolobus solfataricus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CD9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CD9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2cda\|2cda]], [[2cdb\|2cdb]], [[2cdc\|2cdc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2cda\|2cda]], [[2cdb\|2cdb]], [[2cdc\|2cdc]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose_1-dehydrogenase Glucose 1-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.47 1.1.1.47] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose_1-dehydrogenase Glucose 1-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.47 1.1.1.47] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cd9 OCA], [http://pdbe.org/2cd9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cd9 RCSB], [http://www.ebi.ac.uk/pdbsum/2cd9 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cd9 OCA], [http://pdbe.org/2cd9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cd9 RCSB], [http://www.ebi.ac.uk/pdbsum/2cd9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2cd9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cd/2cd9_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cd/2cd9_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Atcc 35091~~]]		[[Category: Saccharolobus solfataricus]]
	[[Category: Glucose 1-dehydrogenase]]		[[Category: Glucose 1-dehydrogenase]]
	[[Category: Danson, M J]]		[[Category: Danson, M J]]

OCA at 01:51, 9 February 2016

2016-02-09T01:51:32Z

← Older revision		Revision as of 01:51, 9 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cd9 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 17:26, 10 September 2015

2015-09-10T17:26:47Z

← Older revision		Revision as of 17:26, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2cd9' size='340' side='right' caption='[[2cd9]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2cd9' size='340' side='right' caption='[[2cd9]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cd9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sulfolobus_solfataricus Sulfolobus solfataricus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CD9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CD9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cd9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CD9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CD9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2cda\|2cda]], [[2cdb\|2cdb]], [[2cdc\|2cdc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2cda\|2cda]], [[2cdb\|2cdb]], [[2cdc\|2cdc]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose_1-dehydrogenase Glucose 1-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.47 1.1.1.47] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose_1-dehydrogenase Glucose 1-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.47 1.1.1.47] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cd9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cd9 RCSB], [http://www.ebi.ac.uk/pdbsum/2cd9 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cd9 OCA], [http://pdbe.org/2cd9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cd9 RCSB], [http://www.ebi.ac.uk/pdbsum/2cd9 PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/GLCDH_SULSF GLCDH_SULSF]] Catalyzes the NAD(P)(+)-dependent oxidation of D-glucose to D-gluconate via gluconolactone. Displays broad substrate specificity since it is able to catalyze the oxidation of a number of alternative aldose sugars, such as D-galactose, D-xylose and L-arabinose, to the corresponding glyconate. Can utilize both NAD(+) and NADP(+) as electron acceptor. Physiologically, seems to be involved in the degradation of both glucose and galactose through a non-phosphorylative variant of the Entner-Doudoroff pathway.<ref>PMID:12824170</ref> <ref>PMID:3827812</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2cd9" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Atcc 35091]]
	[[Category: Glucose 1-dehydrogenase]]		[[Category: Glucose 1-dehydrogenase]]
	~~[[Category: Sulfolobus solfataricus]]~~
	[[Category: Danson, M J]]		[[Category: Danson, M J]]
	[[Category: Hough, D W]]		[[Category: Hough, D W]]

OCA at 18:34, 15 January 2015

2015-01-15T18:34:19Z

← Older revision		Revision as of 18:34, 15 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cd9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CD9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CD9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cd9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CD9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CD9 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2cda\|2cda]], [[2cdb\|2cdb]], [[2cdc\|2cdc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2cda\|2cda]], [[2cdb\|2cdb]], [[2cdc\|2cdc]]</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose_1-dehydrogenase Glucose 1-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.47 1.1.1.47] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose_1-dehydrogenase Glucose 1-dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.47 1.1.1.47] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cd9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cd9 RCSB], [http://www.ebi.ac.uk/pdbsum/2cd9 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cd9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cd9 RCSB], [http://www.ebi.ac.uk/pdbsum/2cd9 PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	[[Category: Glucose 1-dehydrogenase]]		[[Category: Glucose 1-dehydrogenase]]
	[[Category: Sulfolobus solfataricus]]		[[Category: Sulfolobus solfataricus]]
	[[Category: Danson, M J.]]		[[Category: Danson, M J]]
	[[Category: Hough, D W.]]		[[Category: Hough, D W]]
	[[Category: Lamble, H J.]]		[[Category: Lamble, H J]]
	[[Category: Milburn, C C.]]		[[Category: Milburn, C C]]
	[[Category: Taylor, G L.]]		[[Category: Taylor, G L]]
	[[Category: Theodossis, A.]]		[[Category: Theodossis, A]]
	[[Category: Glucose dehydrogenase]]		[[Category: Glucose dehydrogenase]]
	[[Category: Medium chain dehydrogenase family]]		[[Category: Medium chain dehydrogenase family]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 09:08, 3 October 2014

2014-10-03T09:08:13Z

@@ Line 1: / Line 1: @@
-{{STRUCTURE_2cd9|  PDB=2cd9  |  SCENE=  }}
+==SULFOLOBUS SOLFATARICUS GLUCOSE DEHYDROGENASE 1 - APO FORM==
-===SULFOLOBUS SOLFATARICUS GLUCOSE DEHYDROGENASE 1 - APO FORM===
+<StructureSection load='2cd9' size='340' side='right' caption='[[2cd9]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-{{ABSTRACT_PUBMED_16556607}}
+== Structural highlights ==
-==About this Structure==
+The structural basis of substrate promiscuity in glucose dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus.,Milburn CC, Lamble HJ, Theodossis A, Bull SD, Hough DW, Danson MJ, Taylor GL J Biol Chem. 2006 May 26;281(21):14796-804. Epub 2006 Mar 23. PMID:16556607<ref>PMID:16556607</ref>
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<ref group="xtra">PMID:016556607</ref><references group="xtra"/><references/>
+</div>
 [[Category: Glucose 1-dehydrogenase]]
 [[Category: Sulfolobus solfataricus]]

OCA at 08:43, 23 April 2014

2014-04-23T08:43:44Z

← Older revision		Revision as of 08:43, 23 April 2014
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	~~[[Image:2cd9.png\|left\|200px]]~~

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2cd9", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2cd9\| PDB=2cd9 \| SCENE= }}		{{STRUCTURE_2cd9\| PDB=2cd9 \| SCENE= }}

	===SULFOLOBUS SOLFATARICUS GLUCOSE DEHYDROGENASE 1 - APO FORM===		===SULFOLOBUS SOLFATARICUS GLUCOSE DEHYDROGENASE 1 - APO FORM===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_16556607}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 16556607 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_16556607}}		{{ABSTRACT_PUBMED_16556607}}

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	==Reference==		==Reference==
	<ref group="xtra">PMID:016556607</ref><references group="xtra"/>		<ref group="xtra">PMID:016556607</ref><references group="xtra"/><references/>
	[[Category: Glucose 1-dehydrogenase]]		[[Category: Glucose 1-dehydrogenase]]
	[[Category: Sulfolobus solfataricus]]		[[Category: Sulfolobus solfataricus]]