2cfa - Revision history

OCA at 14:15, 13 December 2023

2023-12-13T14:15:49Z

← Older revision		Revision as of 14:15, 13 December 2023
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	<StructureSection load='2cfa' size='340' side='right'caption='[[2cfa]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2cfa' size='340' side='right'caption='[[2cfa]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cfa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Chlorella_pbcv-1_virus~~ Chlorella ~~pbcv-~~1 ~~virus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CFA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CFA FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cfa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Paramecium_bursaria_Chlorella_virus_1 Paramecium bursaria Chlorella virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CFA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CFA FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FAD:FLAVIN~~-~~ADENINE+DINUCLEOTIDE'>FAD</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene><~~/td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Thymidylate_synthase_(~~FAD~~) Thymidylate synthase (FAD)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.148 2.1.1.148]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cfa OCA], [https://pdbe.org/2cfa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cfa RCSB], [https://www.ebi.ac.uk/pdbsum/2cfa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cfa ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cfa OCA], [https://pdbe.org/2cfa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cfa RCSB], [https://www.ebi.ac.uk/pdbsum/2cfa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cfa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/THYX_PBCV1 THYX_PBCV1]] Catalyzes the formation of dTMP and tetrahydrofolate from dUMP and methylenetetrahydrofolate (By similarity).		[https://www.uniprot.org/uniprot/THYX_PBCV1 THYX_PBCV1] Catalyzes the formation of dTMP and tetrahydrofolate from dUMP and methylenetetrahydrofolate (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Chlorella pbcv-1 virus]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bernauer, J]]		[[Category: Paramecium bursaria Chlorella virus 1]]
	[[Category: Decottignies, P]]		[[Category: Bernauer J]]
	[[Category: Graille, M]]		[[Category: Decottignies P]]
	[[Category: Graziani, S]]		[[Category: Graille M]]
	[[Category: Liebl, U]]		[[Category: Graziani S]]
	[[Category: Marchand, C]]		[[Category: Liebl U]]
	[[Category: Myllykallio, H]]		[[Category: Marchand C]]
	[[Category: Skouloubris, S]]		[[Category: Myllykallio H]]
	~~[[Category: Tilbeurgh, H van~~]]		[[Category: Skouloubris S]]
	[[Category: Zhou, C Z]]		[[Category: Zhou C-Z]]
	[[Category: ~~Fdt]]~~		[[Category: Van Tilbeurgh H]]
	~~[[Category: Flavin dependent thymidylate synthase fad]]~~
	~~[[Category: Flavoprotein]]~~
	~~[[Category: Methyltransferase]]~~
	~~[[Category: Nucleotide biosynthesis]]~~
	~~[[Category: Transferase]]~~
	~~[[Category: Tscp~~]]

OCA at 06:54, 1 December 2021

2021-12-01T06:54:23Z

← Older revision		Revision as of 06:54, 1 December 2021
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	<StructureSection load='2cfa' size='340' side='right'caption='[[2cfa]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2cfa' size='340' side='right'caption='[[2cfa]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cfa]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Chlorella_pbcv-1_virus Chlorella pbcv-1 virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CFA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2CFA FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cfa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlorella_pbcv-1_virus Chlorella pbcv-1 virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CFA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CFA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Thymidylate_synthase_(FAD) Thymidylate synthase (FAD)], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.148 2.1.1.148] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Thymidylate_synthase_(FAD) Thymidylate synthase (FAD)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.148 2.1.1.148] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2cfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cfa OCA], [~~http~~://pdbe.org/2cfa PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2cfa RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2cfa PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2cfa ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cfa OCA], [https://pdbe.org/2cfa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cfa RCSB], [https://www.ebi.ac.uk/pdbsum/2cfa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cfa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/THYX_PBCV1 THYX_PBCV1]] Catalyzes the formation of dTMP and tetrahydrofolate from dUMP and methylenetetrahydrofolate (By similarity).		[[https://www.uniprot.org/uniprot/THYX_PBCV1 THYX_PBCV1]] Catalyzes the formation of dTMP and tetrahydrofolate from dUMP and methylenetetrahydrofolate (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	==See Also==		==See Also==
	*[[Thymidylate synthase\|Thymidylate synthase]]		*[[Thymidylate synthase 3D structures\|Thymidylate synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:45, 26 February 2020

2020-02-26T10:45:45Z

← Older revision		Revision as of 10:45, 26 February 2020
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	==Structure of viral flavin-dependant thymidylate synthase ThyX==		==Structure of viral flavin-dependant thymidylate synthase ThyX==
	<StructureSection load='2cfa' size='340' side='right' caption='[[2cfa]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2cfa' size='340' side='right'caption='[[2cfa]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cfa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlorella_pbcv-1_virus Chlorella pbcv-1 virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CFA FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cfa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlorella_pbcv-1_virus Chlorella pbcv-1 virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CFA FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Chlorella pbcv-1 virus]]		[[Category: Chlorella pbcv-1 virus]]
			[[Category: Large Structures]]
	[[Category: Bernauer, J]]		[[Category: Bernauer, J]]
	[[Category: Decottignies, P]]		[[Category: Decottignies, P]]

OCA at 08:00, 23 May 2018

2018-05-23T08:00:34Z

← Older revision		Revision as of 08:00, 23 May 2018
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	==Structure of viral flavin-dependant thymidylate synthase ThyX==		==Structure of viral flavin-dependant thymidylate synthase ThyX==
	<StructureSection load='2cfa' size='340' side='right' caption='[[2cfa]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2cfa' size='340' side='right' caption='[[2cfa]], [[Resolution\|resolution]] 2.30Å' scene=''>
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	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidylate_synthase_(FAD) Thymidylate synthase (FAD)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.148 2.1.1.148] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidylate_synthase_(FAD) Thymidylate synthase (FAD)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.148 2.1.1.148] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cfa OCA], [http://pdbe.org/2cfa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cfa RCSB], [http://www.ebi.ac.uk/pdbsum/2cfa PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cfa OCA], [http://pdbe.org/2cfa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cfa RCSB], [http://www.ebi.ac.uk/pdbsum/2cfa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2cfa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/2cfa_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/2cfa_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 10:12, 7 February 2016

2016-02-07T10:12:01Z

← Older revision		Revision as of 10:12, 7 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cfa ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 00:30, 10 September 2015

2015-09-10T00:30:11Z

← Older revision		Revision as of 00:30, 10 September 2015
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	<StructureSection load='2cfa' size='340' side='right' caption='[[2cfa]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2cfa' size='340' side='right' caption='[[2cfa]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cfa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella~~ virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CFA FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cfa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlorella_pbcv-1_virus Chlorella pbcv-1 virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CFA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidylate_synthase_(FAD) Thymidylate synthase (FAD)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.148 2.1.1.148] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidylate_synthase_(FAD) Thymidylate synthase (FAD)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.148 2.1.1.148] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cfa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cfa RCSB], [http://www.ebi.ac.uk/pdbsum/2cfa PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cfa OCA], [http://pdbe.org/2cfa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cfa RCSB], [http://www.ebi.ac.uk/pdbsum/2cfa PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2cfa" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Paramecium bursaria chlorella~~ virus 1]]		[[Category: Chlorella pbcv-1 virus]]
	[[Category: Bernauer, J]]		[[Category: Bernauer, J]]
	[[Category: Decottignies, P]]		[[Category: Decottignies, P]]

OCA at 14:19, 24 December 2014

2014-12-24T14:19:24Z

← Older revision		Revision as of 14:19, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cfa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CFA FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cfa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CFA FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene></td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidylate_synthase_(FAD) Thymidylate synthase (FAD)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.148 2.1.1.148] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidylate_synthase_(FAD) Thymidylate synthase (FAD)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.148 2.1.1.148] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cfa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cfa RCSB], [http://www.ebi.ac.uk/pdbsum/2cfa PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cfa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cfa RCSB], [http://www.ebi.ac.uk/pdbsum/2cfa PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/THYX_PBCV1 THYX_PBCV1]] Catalyzes the formation of dTMP and tetrahydrofolate from dUMP and methylenetetrahydrofolate (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Paramecium bursaria chlorella virus 1]]		[[Category: Paramecium bursaria chlorella virus 1]]
	[[Category: Bernauer, J.]]		[[Category: Bernauer, J]]
	[[Category: Decottignies, P.]]		[[Category: Decottignies, P]]
	[[Category: Graille, M.]]		[[Category: Graille, M]]
	[[Category: Graziani, S.]]		[[Category: Graziani, S]]
	[[Category: Liebl, U.]]		[[Category: Liebl, U]]
	[[Category: Marchand, C.]]		[[Category: Marchand, C]]
	[[Category: Myllykallio, H.]]		[[Category: Myllykallio, H]]
	[[Category: Skouloubris, S.]]		[[Category: Skouloubris, S]]
	[[Category: Tilbeurgh, H van.]]		[[Category: Tilbeurgh, H van]]
	[[Category: Zhou, C Z.]]		[[Category: Zhou, C Z]]
	[[Category: Fdt]]		[[Category: Fdt]]
	[[Category: Flavin dependent thymidylate synthase fad]]		[[Category: Flavin dependent thymidylate synthase fad]]

OCA at 04:03, 3 October 2014

2014-10-03T04:03:55Z

← Older revision		Revision as of 04:03, 3 October 2014
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	~~{{STRUCTURE_2cfa~~\| ~~PDB~~=2cfa \| ~~SCENE~~= }}		==Structure of viral flavin-dependant thymidylate synthase ThyX==
	===~~Structure~~ of ~~viral~~ flavin-~~dependant~~ thymidylate synthase ThyX~~===~~		<StructureSection load='2cfa' size='340' side='right' caption='[[2cfa]], [[Resolution\|resolution]] 2.30Å' scene=''>
	~~{{ABSTRACT_PUBMED_16707489}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[2cfa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CFA FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene><br>
			<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene></td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidylate_synthase_(FAD) Thymidylate synthase (FAD)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.148 2.1.1.148] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cfa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cfa RCSB], [http://www.ebi.ac.uk/pdbsum/2cfa PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/2cfa_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			By using biochemical and structural analyses, we have investigated the catalytic mechanism of the recently discovered flavin-dependent thymidylate synthase ThyX from Paramecium bursaria chlorella virus-1 (PBCV-1). Site-directed mutagenesis experiments have identified several residues implicated in either NADPH oxidation or deprotonation activity of PBCV-1 ThyX. Chemical modification by diethyl pyrocarbonate and mass spectroscopic analyses identified a histidine residue (His53) crucial for NADPH oxidation and located in the vicinity of the redox active N-5 atom of the FAD ring system. Moreover, we observed that the conformation of active site key residues of PBCV-1 ThyX differs from earlier reported ThyX structures, suggesting structural changes during catalysis. Steady-state kinetic analyses support a reaction mechanism where ThyX catalysis proceeds via formation of distinct ternary complexes without formation of a methyl enzyme intermediate.

	~~==Function==~~		Catalytic mechanism and structure of viral flavin-dependent thymidylate synthase ThyX.,Graziani S, Bernauer J, Skouloubris S, Graille M, Zhou CZ, Marchand C, Decottignies P, van Tilbeurgh H, Myllykallio H, Liebl U J Biol Chem. 2006 Aug 18;281(33):24048-57. Epub 2006 May 17. PMID:16707489<ref>PMID:16707489</ref>
	~~[[http://www~~.~~uniprot~~.~~org/uniprot/THYX_PBCV1 THYX_PBCV1]] Catalyzes the formation of dTMP and tetrahydrofolate from dUMP and methylenetetrahydrofolate~~ (~~By similarity~~).

	~~==About this Structure==~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~[[2cfa]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1]~~. ~~Full crystallographic information is available from [http:/~~/~~oca.weizmann.ac.il/oca-bin/ocashort?id=2CFA OCA].~~		</div>

	==See Also==		==See Also==
	*[[Thymidylate synthase\|Thymidylate synthase]]		*[[Thymidylate synthase\|Thymidylate synthase]]
			== References ==
	==~~Reference~~==		<references/>
	~~<ref group="xtra">PMID:016707489</ref>~~<references ~~group="xtra"~~/><~~references~~/>		__TOC__
			</StructureSection>
	[[Category: Paramecium bursaria chlorella virus 1]]		[[Category: Paramecium bursaria chlorella virus 1]]
	[[Category: Bernauer, J.]]		[[Category: Bernauer, J.]]

OCA at 04:07, 18 July 2013

2013-07-18T04:07:42Z

← Older revision		Revision as of 04:07, 18 July 2013
Line 1:		Line 1:
	~~[[Image:2cfa.png\|left\|200px]]~~

	{{STRUCTURE_2cfa\| PDB=2cfa \| SCENE= }}		{{STRUCTURE_2cfa\| PDB=2cfa \| SCENE= }}
			===Structure of viral flavin-dependant thymidylate synthase ThyX===
			{{ABSTRACT_PUBMED_16707489}}

	===~~STRUCTURE OF VIRAL FLAVIN-DEPENDANT THYMIDYLATE SYNTHASE THYX~~===		==Function==
			[[http://www.uniprot.org/uniprot/THYX_PBCV1 THYX_PBCV1]] Catalyzes the formation of dTMP and tetrahydrofolate from dUMP and methylenetetrahydrofolate (By similarity).
	~~{{ABSTRACT_PUBMED_16707489}}~~

	==About this Structure==		==About this Structure==
Line 14:		Line 13:

	==Reference==		==Reference==
	<ref group="xtra">PMID:016707489</ref><references group="xtra"/>		<ref group="xtra">PMID:016707489</ref><references group="xtra"/><references/>
	[[Category: Paramecium bursaria chlorella virus 1]]		[[Category: Paramecium bursaria chlorella virus 1]]
	[[Category: Bernauer, J.]]		[[Category: Bernauer, J.]]
Line 24:		Line 23:
	[[Category: Myllykallio, H.]]		[[Category: Myllykallio, H.]]
	[[Category: Skouloubris, S.]]		[[Category: Skouloubris, S.]]
	[[Category: Tilbeurgh, H ~~Van~~.]]		[[Category: Tilbeurgh, H van.]]
	[[Category: Zhou, C Z.]]		[[Category: Zhou, C Z.]]
	[[Category: Fdt]]		[[Category: Fdt]]
Line 31:		Line 30:
	[[Category: Methyltransferase]]		[[Category: Methyltransferase]]
	[[Category: Nucleotide biosynthesis]]		[[Category: Nucleotide biosynthesis]]
	~~[[Category: Paramecium bursaria chlorella virus-1]]~~
	~~[[Category: Thyx]]~~
	[[Category: Transferase]]		[[Category: Transferase]]
	[[Category: Tscp]]		[[Category: Tscp]]

OCA at 06:20, 20 October 2012

2012-10-20T06:20:27Z

← Older revision		Revision as of 06:20, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[2cfa]] is a 2 chain structure ~~of [[Thymidylate synthase]]~~ with sequence from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CFA OCA].		[[2cfa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CFA OCA].

	==See Also==		==See Also==