2cli - Revision history

OCA at 10:24, 8 February 2016

2016-02-08T10:24:02Z

← Older revision		Revision as of 10:24, 8 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cli ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:38, 10 September 2015

2015-09-10T10:38:08Z

← Older revision		Revision as of 10:38, 10 September 2015
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	<StructureSection load='2cli' size='340' side='right' caption='[[2cli]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='2cli' size='340' side='right' caption='[[2cli]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cli]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CLI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CLI FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cli]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CLI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CLI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F9F:2-({[4-(TRIFLUOROMETHOXY)PHENYL]SULFONYL}AMINO)ETHYL+DIHYDROGEN+PHOSPHATE'>F9F</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F9F:2-({[4-(TRIFLUOROMETHOXY)PHENYL]SULFONYL}AMINO)ETHYL+DIHYDROGEN+PHOSPHATE'>F9F</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1a50\|1a50]], [[1a5a\|1a5a]], [[1a5b\|1a5b]], [[1a5s\|1a5s]], [[1bks\|1bks]], [[1c29\|1c29]], [[1c8v\|1c8v]], [[1c9d\|1c9d]], [[1cw2\|1cw2]], [[1cx9\|1cx9]], [[1fuy\|1fuy]], [[1geq\|1geq]], [[1k3u\|1k3u]], [[1k7e\|1k7e]], [[1k7f\|1k7f]], [[1k7x\|1k7x]], [[1k8y\|1k8y]], [[1k8z\|1k8z]], [[1kfj\|1kfj]], [[1kfk\|1kfk]], [[1qop\|1qop]], [[1qoq\|1qoq]], [[1rd5\|1rd5]], [[1tjp\|1tjp]], [[1ttp\|1ttp]], [[1ttq\|1ttq]], [[1ubs\|1ubs]], [[1ujp\|1ujp]], [[1v7y\|1v7y]], [[1v8z\|1v8z]], [[1wbj\|1wbj]], [[1wq5\|1wq5]], [[1wxj\|1wxj]], [[1xc4\|1xc4]], [[1xcf\|1xcf]], [[2cle\|2cle]], [[2clf\|2clf]], [[2clh\|2clh]], [[2clk\|2clk]], [[2cll\|2cll]], [[2clm\|2clm]], [[2clo\|2clo]], [[2trs\|2trs]], [[2tsy\|2tsy]], [[2tys\|2tys]], [[2wsy\|2wsy]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1a50\|1a50]], [[1a5a\|1a5a]], [[1a5b\|1a5b]], [[1a5s\|1a5s]], [[1bks\|1bks]], [[1c29\|1c29]], [[1c8v\|1c8v]], [[1c9d\|1c9d]], [[1cw2\|1cw2]], [[1cx9\|1cx9]], [[1fuy\|1fuy]], [[1geq\|1geq]], [[1k3u\|1k3u]], [[1k7e\|1k7e]], [[1k7f\|1k7f]], [[1k7x\|1k7x]], [[1k8y\|1k8y]], [[1k8z\|1k8z]], [[1kfj\|1kfj]], [[1kfk\|1kfk]], [[1qop\|1qop]], [[1qoq\|1qoq]], [[1rd5\|1rd5]], [[1tjp\|1tjp]], [[1ttp\|1ttp]], [[1ttq\|1ttq]], [[1ubs\|1ubs]], [[1ujp\|1ujp]], [[1v7y\|1v7y]], [[1v8z\|1v8z]], [[1wbj\|1wbj]], [[1wq5\|1wq5]], [[1wxj\|1wxj]], [[1xc4\|1xc4]], [[1xcf\|1xcf]], [[2cle\|2cle]], [[2clf\|2clf]], [[2clh\|2clh]], [[2clk\|2clk]], [[2cll\|2cll]], [[2clm\|2clm]], [[2clo\|2clo]], [[2trs\|2trs]], [[2tsy\|2tsy]], [[2tys\|2tys]], [[2wsy\|2wsy]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cli OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cli RCSB], [http://www.ebi.ac.uk/pdbsum/2cli PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cli OCA], [http://pdbe.org/2cli PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cli RCSB], [http://www.ebi.ac.uk/pdbsum/2cli PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2cli" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]~~
	[[Category: Tryptophan synthase]]		[[Category: Tryptophan synthase]]
	[[Category: Barends, T R]]		[[Category: Barends, T R]]

OCA at 22:39, 24 December 2014

2014-12-24T22:39:39Z

← Older revision		Revision as of 22:39, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cli]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CLI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CLI FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cli]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CLI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CLI FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F9F:2-({[4-(TRIFLUOROMETHOXY)PHENYL]SULFONYL}AMINO)ETHYL+DIHYDROGEN+PHOSPHATE'>F9F</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F9F:2-({[4-(TRIFLUOROMETHOXY)PHENYL]SULFONYL}AMINO)ETHYL+DIHYDROGEN+PHOSPHATE'>F9F</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1a50\|1a50]], [[1a5a\|1a5a]], [[1a5b\|1a5b]], [[1a5s\|1a5s]], [[1bks\|1bks]], [[1c29\|1c29]], [[1c8v\|1c8v]], [[1c9d\|1c9d]], [[1cw2\|1cw2]], [[1cx9\|1cx9]], [[1fuy\|1fuy]], [[1geq\|1geq]], [[1k3u\|1k3u]], [[1k7e\|1k7e]], [[1k7f\|1k7f]], [[1k7x\|1k7x]], [[1k8y\|1k8y]], [[1k8z\|1k8z]], [[1kfj\|1kfj]], [[1kfk\|1kfk]], [[1qop\|1qop]], [[1qoq\|1qoq]], [[1rd5\|1rd5]], [[1tjp\|1tjp]], [[1ttp\|1ttp]], [[1ttq\|1ttq]], [[1ubs\|1ubs]], [[1ujp\|1ujp]], [[1v7y\|1v7y]], [[1v8z\|1v8z]], [[1wbj\|1wbj]], [[1wq5\|1wq5]], [[1wxj\|1wxj]], [[1xc4\|1xc4]], [[1xcf\|1xcf]], [[2cle\|2cle]], [[2clf\|2clf]], [[2clh\|2clh]], [[2clk\|2clk]], [[2cll\|2cll]], [[2clm\|2clm]], [[2clo\|2clo]], [[2trs\|2trs]], [[2tsy\|2tsy]], [[2tys\|2tys]], [[2wsy\|2wsy]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1a50\|1a50]], [[1a5a\|1a5a]], [[1a5b\|1a5b]], [[1a5s\|1a5s]], [[1bks\|1bks]], [[1c29\|1c29]], [[1c8v\|1c8v]], [[1c9d\|1c9d]], [[1cw2\|1cw2]], [[1cx9\|1cx9]], [[1fuy\|1fuy]], [[1geq\|1geq]], [[1k3u\|1k3u]], [[1k7e\|1k7e]], [[1k7f\|1k7f]], [[1k7x\|1k7x]], [[1k8y\|1k8y]], [[1k8z\|1k8z]], [[1kfj\|1kfj]], [[1kfk\|1kfk]], [[1qop\|1qop]], [[1qoq\|1qoq]], [[1rd5\|1rd5]], [[1tjp\|1tjp]], [[1ttp\|1ttp]], [[1ttq\|1ttq]], [[1ubs\|1ubs]], [[1ujp\|1ujp]], [[1v7y\|1v7y]], [[1v8z\|1v8z]], [[1wbj\|1wbj]], [[1wq5\|1wq5]], [[1wxj\|1wxj]], [[1xc4\|1xc4]], [[1xcf\|1xcf]], [[2cle\|2cle]], [[2clf\|2clf]], [[2clh\|2clh]], [[2clk\|2clk]], [[2cll\|2cll]], [[2clm\|2clm]], [[2clo\|2clo]], [[2trs\|2trs]], [[2tsy\|2tsy]], [[2tys\|2tys]], [[2wsy\|2wsy]]</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cli OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cli RCSB], [http://www.ebi.ac.uk/pdbsum/2cli PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cli OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cli RCSB], [http://www.ebi.ac.uk/pdbsum/2cli PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/TRPA_SALTY TRPA_SALTY]] The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate. [[http://www.uniprot.org/uniprot/TRPB_SALTY TRPB_SALTY]] The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]		[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
	[[Category: Tryptophan synthase]]		[[Category: Tryptophan synthase]]
	[[Category: Barends, T R.]]		[[Category: Barends, T R]]
	[[Category: Blumenstein, L.]]		[[Category: Blumenstein, L]]
	[[Category: Casino, P.]]		[[Category: Casino, P]]
	[[Category: Dunn, M F.]]		[[Category: Dunn, M F]]
	[[Category: Harris, R.]]		[[Category: Harris, R]]
	[[Category: Kimmich, N.]]		[[Category: Kimmich, N]]
	[[Category: Kulik, V.]]		[[Category: Kulik, V]]
	[[Category: Ngo, H.]]		[[Category: Ngo, H]]
	[[Category: Niks, D.]]		[[Category: Niks, D]]
	[[Category: Schlichting, I.]]		[[Category: Schlichting, I]]
	[[Category: Weyand, M.]]		[[Category: Weyand, M]]
	[[Category: Allosteric enzyme]]		[[Category: Allosteric enzyme]]
	[[Category: Amino-acid biosynthesis]]		[[Category: Amino-acid biosynthesis]]

OCA at 00:34, 30 September 2014

2014-09-30T00:34:55Z

← Older revision		Revision as of 00:34, 30 September 2014
Line 1:		Line 1:
	[[Image:~~2cli~~.~~png~~\|~~left~~\|~~200px~~]]		==TRYPTOPHAN SYNTHASE IN COMPLEX WITH N-(4'-TRIFLUOROMETHOXYBENZENESULFONYL)-2-AMINO-1-ETHYLPHOSPHATE (F9)==
			<StructureSection load='2cli' size='340' side='right' caption='[[2cli]], [[Resolution\|resolution]] 1.70Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2cli]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CLI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CLI FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F9F:2-({[4-(TRIFLUOROMETHOXY)PHENYL]SULFONYL}AMINO)ETHYL+DIHYDROGEN+PHOSPHATE'>F9F</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1a50\|1a50]], [[1a5a\|1a5a]], [[1a5b\|1a5b]], [[1a5s\|1a5s]], [[1bks\|1bks]], [[1c29\|1c29]], [[1c8v\|1c8v]], [[1c9d\|1c9d]], [[1cw2\|1cw2]], [[1cx9\|1cx9]], [[1fuy\|1fuy]], [[1geq\|1geq]], [[1k3u\|1k3u]], [[1k7e\|1k7e]], [[1k7f\|1k7f]], [[1k7x\|1k7x]], [[1k8y\|1k8y]], [[1k8z\|1k8z]], [[1kfj\|1kfj]], [[1kfk\|1kfk]], [[1qop\|1qop]], [[1qoq\|1qoq]], [[1rd5\|1rd5]], [[1tjp\|1tjp]], [[1ttp\|1ttp]], [[1ttq\|1ttq]], [[1ubs\|1ubs]], [[1ujp\|1ujp]], [[1v7y\|1v7y]], [[1v8z\|1v8z]], [[1wbj\|1wbj]], [[1wq5\|1wq5]], [[1wxj\|1wxj]], [[1xc4\|1xc4]], [[1xcf\|1xcf]], [[2cle\|2cle]], [[2clf\|2clf]], [[2clh\|2clh]], [[2clk\|2clk]], [[2cll\|2cll]], [[2clm\|2clm]], [[2clo\|2clo]], [[2trs\|2trs]], [[2tsy\|2tsy]], [[2tys\|2tys]], [[2wsy\|2wsy]]</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cli OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cli RCSB], [http://www.ebi.ac.uk/pdbsum/2cli PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cl/2cli_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Allosteric interactions regulate substrate channeling in Salmonella typhimurium tryptophan synthase. The channeling of indole between the alpha- and beta-sites via the interconnecting 25 A tunnel is regulated by allosteric signaling arising from binding of ligand to the alpha-site, and covalent reaction of l-Ser at the beta-site. This signaling switches the alpha- and beta-subunits between open conformations of low activity and closed conformations of high activity. Our objective is to synthesize and characterize new classes of alpha-site ligands (ASLs) that mimic the binding of substrates, 3-indole-d-glycerol 3'-phosphate (IGP) or d-glyceraldehyde 3-phosphate (G3P), for use in the investigation of alpha-site-beta-site interactions. The new synthesized IGP analogues contain an aryl group linked to an O-phosphoethanolamine moiety through amide, sulfonamide, or thiourea groups. The G3P analogue, thiophosphoglycolohydroxamate, contains a hydroxamic acid group linked to a thiophosphate moiety. Crystal structures of the internal aldimine complexed with G3P and with three of the new ASLs are presented. These structural and solution studies of the ASL complexes with the internal aldimine form of the enzyme establish the following. (1) ASL binding occurs with high specificity and relatively high affinities at the alpha-site. (2) Binding of the new ASLs slows the entry of indole analogues into the beta-site by blocking the tunnel opening at the alpha-site. (3) ASL binding stabilizes the closed conformations of the beta-subunit for the alpha-aminoacrylate and quinonoid forms of the enzyme. (4) The new ASLs exhibit allosteric properties that parallel the behaviors of IGP and G3P.

	~~{{STRUCTURE_2cli\| PDB=2cli \| SCENE= }}~~		Synthesis and characterization of allosteric probes of substrate channeling in the tryptophan synthase bienzyme complex.,Ngo H, Harris R, Kimmich N, Casino P, Niks D, Blumenstein L, Barends TR, Kulik V, Weyand M, Schlichting I, Dunn MF Biochemistry. 2007 Jul 3;46(26):7713-27. Epub 2007 Jun 9. PMID:17559195<ref>PMID:17559195</ref>

	~~===TRYPTOPHAN SYNTHASE IN COMPLEX WITH N-(4'-TRIFLUOROMETHOXYBENZENESULFONYL)-2-AMINO-1-ETHYLPHOSPHATE (F9)===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_17559195}}~~

	~~==About this Structure==~~
	~~[[2cli]] is~~ a ~~2 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp~~.~~_enterica_serovar_typhimurium Salmonella enterica subsp~~. ~~enterica serovar typhimurium]~~. ~~Full crystallographic information is available from [http://oca.weizmann.ac.il~~/~~oca-bin/ocashort?id=2CLI OCA].~~

	==See Also==		==See Also==
	*[[Tryptophan synthase\|Tryptophan synthase]]		*[[Tryptophan synthase\|Tryptophan synthase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:017559195<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]		[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
	[[Category: Tryptophan synthase]]		[[Category: Tryptophan synthase]]

OCA: Protected "2cli" [edit=sysop:move=sysop]

2013-01-06T09:57:00Z

Protected "2cli" [edit=sysop:move=sysop]

← Older revision	Revision as of 09:57, 6 January 2013
(No difference)

OCA at 09:56, 6 January 2013

2013-01-06T09:56:58Z

← Older revision		Revision as of 09:56, 6 January 2013
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	~~{{Seed}}~~
	[[Image:2cli.png\|left\|200px]]		[[Image:2cli.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2cli", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2cli\| PDB=2cli \| SCENE= }}		{{STRUCTURE_2cli\| PDB=2cli \| SCENE= }}

	===TRYPTOPHAN SYNTHASE IN COMPLEX WITH N-(4'-TRIFLUOROMETHOXYBENZENESULFONYL)-2-AMINO-1-ETHYLPHOSPHATE (F9)===		===TRYPTOPHAN SYNTHASE IN COMPLEX WITH N-(4'-TRIFLUOROMETHOXYBENZENESULFONYL)-2-AMINO-1-ETHYLPHOSPHATE (F9)===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_17559195}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 17559195 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_17559195}}		{{ABSTRACT_PUBMED_17559195}}

	==About this Structure==		==About this Structure==
	~~2CLI~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/~~Salmonella_typhimurium~~ Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CLI OCA].		[[2cli]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CLI OCA].

			==See Also==
			*[[Tryptophan synthase\|Tryptophan synthase]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~17559195~~</ref><references group="xtra"/>		<ref group="xtra">PMID:017559195</ref><references group="xtra"/>
	[[Category: Salmonella typhimurium]]		[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
	[[Category: Tryptophan synthase]]		[[Category: Tryptophan synthase]]
	[[Category: Barends, T R.]]		[[Category: Barends, T R.]]
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	[[Category: Pyridoxal phosphate]]		[[Category: Pyridoxal phosphate]]
	[[Category: Tryptophan biosynthesis]]		[[Category: Tryptophan biosynthesis]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 21:18:41 2009''~~

OCA at 19:18, 16 February 2009

2009-02-16T19:18:44Z

← Older revision		Revision as of 19:18, 16 February 2009
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	==About this Structure==		==About this Structure==
	2CLI is a ~~[[Protein complex]]~~ structure of sequences from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CLI OCA].		2CLI is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CLI OCA].

	==Reference==		==Reference==
	Synthesis and characterization of allosteric probes of substrate channeling in the tryptophan synthase bienzyme complex., Ngo H, Harris R, Kimmich N, Casino P, Niks D, Blumenstein L, Barends TR, Kulik V, Weyand M, Schlichting I, Dunn MF, Biochemistry. 2007 Jul 3;46(26):7713-27. Epub 2007 Jun 9. PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~17559195 17559195]~~		<ref group="xtra">PMID:17559195</ref><references group="xtra"/>
	~~[[Category: Protein complex]]~~
	[[Category: Salmonella typhimurium]]		[[Category: Salmonella typhimurium]]
	[[Category: Tryptophan synthase]]		[[Category: Tryptophan synthase]]
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	[[Category: Tryptophan biosynthesis]]		[[Category: Tryptophan biosynthesis]]

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OCA at 23:09, 27 July 2008

2008-07-27T23:09:51Z

← Older revision		Revision as of 23:09, 27 July 2008
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	[[Image:2cli.~~jpg~~\|left\|200px]]		{{Seed}}
			[[Image:2cli.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_2cli\| PDB=2cli \| SCENE= }}		{{STRUCTURE_2cli\| PDB=2cli \| SCENE= }}

	~~'''~~TRYPTOPHAN SYNTHASE IN COMPLEX WITH N-(4'-TRIFLUOROMETHOXYBENZENESULFONYL)-2-AMINO-1-ETHYLPHOSPHATE (F9)~~'''~~		===TRYPTOPHAN SYNTHASE IN COMPLEX WITH N-(4'-TRIFLUOROMETHOXYBENZENESULFONYL)-2-AMINO-1-ETHYLPHOSPHATE (F9)===


	~~==Overview==~~		<!--
	~~Allosteric interactions regulate substrate channeling in Salmonella typhimurium tryptophan synthase.~~ The channeling of indole between the alpha- and beta-sites via the interconnecting 25 A tunnel is regulated by allosteric signaling arising from binding of ligand to the alpha-site, and covalent reaction of l-Ser at the beta-site. This signaling switches the alpha- and beta-subunits between open conformations of low activity and closed conformations of high activity. Our objective is to synthesize and characterize new classes of alpha-site ligands (ASLs) that mimic the binding of substrates, ~~3-indole-d-glycerol 3'-phosphate (IGP) or d-glyceraldehyde 3-phosphate (G3P)~~, ~~for use in~~ the ~~investigation of alpha-site-beta-site interactions. The new synthesized IGP analogues contain an aryl group linked~~ to an O-phosphoethanolamine moiety through amide, sulfonamide, or thiourea groups. The G3P analogue, thiophosphoglycolohydroxamate, contains a hydroxamic acid group linked to a thiophosphate moiety. Crystal structures of the internal aldimine complexed with G3P and with three of the new ASLs are presented. These structural and solution studies of the ASL complexes with the internal aldimine form of the enzyme establish the following. (~~1) ASL binding occurs with high specificity and relatively high affinities~~ at ~~the alpha-site~~. (2) ~~Binding of~~ the ~~new ASLs slows the entry of indole analogues into the beta-site by blocking the tunnel opening at the alpha-site~~. ~~(3) ASL binding stabilizes the closed conformations of the beta~~-~~subunit for the alpha~~-~~aminoacrylate and quinonoid forms of the enzyme. (4) The new ASLs exhibit allosteric properties that parallel the behaviors of IGP and G3P.~~		The line below this paragraph, {{ABSTRACT_PUBMED_17559195}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 17559195 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_17559195}}

	==About this Structure==		==About this Structure==
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	[[Category: Pyridoxal phosphate]]		[[Category: Pyridoxal phosphate]]
	[[Category: Tryptophan biosynthesis]]		[[Category: Tryptophan biosynthesis]]
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OCA at 19:25, 3 May 2008

2008-05-03T19:25:54Z

← Older revision		Revision as of 19:25, 3 May 2008
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	[[Image:2cli.jpg\|left\|200px]]		[[Image:2cli.jpg\|left\|200px]]

	~~{{Structure~~		<!--
	~~\|PDB= 2cli \|SIZE=350\|CAPTION= <scene name='initialview01'>2cli</scene>~~, ~~resolution 1~~.~~70Å~~		The line below this paragraph, containing "STRUCTURE_2cli", creates the "Structure Box" on the page.
	~~\|SITE= <scene name='pdbsite=AC1:Na+Binding+Site+For+Chain+A'>AC1</scene>~~		You may change the PDB parameter (which sets the PDB file loaded into the applet)
	~~\|LIGAND= <~~scene ~~name='pdbligand=F9F:2-({[4-(TRIFLUOROMETHOXY)PHENYL]SULFONYL}AMINO~~)~~ETHYL+DIHYDROGEN+PHOSPHATE'>F9F</scene>~~, ~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5'-PHOSPHATE'>PLP</scene>~~		or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
	~~\|ACTIVITY= <span class='plainlinks'>[http://en~~.~~wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase], with EC number [http://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20] </span~~>		or leave the SCENE parameter empty for the default display.
	\|~~GENE~~=		-->
	\|~~DOMAIN~~=		{{STRUCTURE_2cli\| PDB=2cli \| SCENE= }}
	~~\|RELATEDENTRY=~~
	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cli OCA], [http://www.ebi.ac.uk/pdbsum/2cli PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2cli RCSB]</span>
	}}

	'''TRYPTOPHAN SYNTHASE IN COMPLEX WITH N-(4'-TRIFLUOROMETHOXYBENZENESULFONYL)-2-AMINO-1-ETHYLPHOSPHATE (F9)'''		'''TRYPTOPHAN SYNTHASE IN COMPLEX WITH N-(4'-TRIFLUOROMETHOXYBENZENESULFONYL)-2-AMINO-1-ETHYLPHOSPHATE (F9)'''
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	[[Category: Schlichting, I.]]		[[Category: Schlichting, I.]]
	[[Category: Weyand, M.]]		[[Category: Weyand, M.]]
	[[Category: ~~allosteric~~ enzyme]]		[[Category: Allosteric enzyme]]
	[[Category: ~~amino~~-acid biosynthesis]]		[[Category: Amino-acid biosynthesis]]
	[[Category: ~~aromatic~~ amino acid biosynthesis]]		[[Category: Aromatic amino acid biosynthesis]]
	[[Category: ~~carbon~~- oxygen lyase]]		[[Category: Carbon- oxygen lyase]]
	[[Category: ~~lyase~~]]		[[Category: Lyase]]
	[[Category: ~~pyridoxal~~ phosphate]]		[[Category: Pyridoxal phosphate]]
	[[Category: ~~tryptophan~~ biosynthesis]]		[[Category: Tryptophan biosynthesis]]
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	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Mar 31 02~~:23:12 2008''

OCA at 23:23, 30 March 2008

2008-03-30T23:23:13Z

← Older revision		Revision as of 23:23, 30 March 2008
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	\|PDB= 2cli \|SIZE=350\|CAPTION= <scene name='initialview01'>2cli</scene>, resolution 1.70Å		\|PDB= 2cli \|SIZE=350\|CAPTION= <scene name='initialview01'>2cli</scene>, resolution 1.70Å
	\|SITE= <scene name='pdbsite=AC1:Na+Binding+Site+For+Chain+A'>AC1</scene>		\|SITE= <scene name='pdbsite=AC1:Na+Binding+Site+For+Chain+A'>AC1</scene>
	\|LIGAND= ~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene>,~~ <scene name='pdbligand=F9F:2-({[4-(TRIFLUOROMETHOXY)PHENYL]SULFONYL}AMINO)ETHYL+DIHYDROGEN+PHOSPHATE'>F9F</scene> ~~and~~ <scene name='pdbligand=PLP:PYRIDOXAL-5'-PHOSPHATE'>PLP</scene>		\|LIGAND= <scene name='pdbligand=F9F:2-({[4-(TRIFLUOROMETHOXY)PHENYL]SULFONYL}AMINO)ETHYL+DIHYDROGEN+PHOSPHATE'>F9F</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5'-PHOSPHATE'>PLP</scene>
	\|ACTIVITY= [http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20]		\|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20] </span>
	\|GENE=		\|GENE=
			\|DOMAIN=
			\|RELATEDENTRY=
			\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cli OCA], [http://www.ebi.ac.uk/pdbsum/2cli PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2cli RCSB]</span>
	}}		}}

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	[[Category: Schlichting, I.]]		[[Category: Schlichting, I.]]
	[[Category: Weyand, M.]]		[[Category: Weyand, M.]]
	~~[[Category: F9F]]~~
	~~[[Category: NA]]~~
	~~[[Category: PLP]]~~
	[[Category: allosteric enzyme]]		[[Category: allosteric enzyme]]
	[[Category: amino-acid biosynthesis]]		[[Category: amino-acid biosynthesis]]
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	[[Category: tryptophan biosynthesis]]		[[Category: tryptophan biosynthesis]]

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