2cwt - Revision history

OCA at 11:31, 22 May 2024

2024-05-22T11:31:53Z

← Older revision		Revision as of 11:31, 22 May 2024
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	<StructureSection load='2cwt' size='340' side='right'caption='[[2cwt]], [[Resolution\|resolution]] 1.82Å' scene=''>		<StructureSection load='2cwt' size='340' side='right'caption='[[2cwt]], [[Resolution\|resolution]] 1.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cwt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"achromobacter_globiformis"_(conn_1928)_bergey_et_al._1930 "achromobacter~~ globiformis~~" (conn 1928) bergey et al. 1930~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CWT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cwt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arthrobacter_globiformis Arthrobacter globiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CWT FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.82Å</td></tr>
	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPQ:5-(2-CARBOXY-2-AMINOETHYL)-2-HYDROXY-1,4-BENZOQUINONE'>TPQ</scene~~></td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=TPQ:5-(2-CARBOXY-2-AMINOETHYL)-2-HYDROXY-1,4-BENZOQUINONE'>TPQ</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1iu7\|1iu7]], [[2cwu\|2cwu]], [[2cwv\|2cwv]]</div></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Oxidoreductase Oxidoreductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.21 and 1.4.3.22 1.4.3.21 and 1.4.3.22] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cwt OCA], [https://pdbe.org/2cwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cwt RCSB], [https://www.ebi.ac.uk/pdbsum/2cwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cwt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cwt OCA], [https://pdbe.org/2cwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cwt RCSB], [https://www.ebi.ac.uk/pdbsum/2cwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cwt ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/PAOX_ARTGO PAOX_ARTGO]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Arthrobacter globiformis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	~~[[Category: Oxidoreductase]]~~		[[Category: Chiu YC]]
	[[Category: Chiu~~, Y C~~]]		[[Category: Hayashi H]]
	[[Category: Hayashi, H]]		[[Category: Hirota S]]
	[[Category: Hirota, S]]		[[Category: Kamiya N]]
	[[Category: Kamiya, N]]		[[Category: Kawano Y]]
	[[Category: Kawano, Y]]		[[Category: Kim M]]
	[[Category: Kim, M]]		[[Category: Kuroda S]]
	[[Category: Kuroda, S]]		[[Category: Murakawa T]]
	[[Category: Murakawa, T]]		[[Category: Okajima T]]
	[[Category: Okajima, T]]		[[Category: Taki M]]
	[[Category: Taki, M]]		[[Category: Tanizawa K]]
	[[Category: Tanizawa, K]]		[[Category: Uchida M]]
	[[Category: Uchida, M]]		[[Category: Yamaguchi H]]
	[[Category: Yamaguchi, H]]		[[Category: Yamamoto Y]]
	[[Category: Yamamoto, Y]]
	~~[[Category: Amine oxidase]]~~
	~~[[Category: Catalytic base]]~~
	~~[[Category: Copper]]~~
	~~[[Category: Topaquinone]]~~
	~~[[Category: Tpq~~]]

OCA at 06:47, 10 November 2021

2021-11-10T06:47:25Z

← Older revision		Revision as of 06:47, 10 November 2021
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	<StructureSection load='2cwt' size='340' side='right'caption='[[2cwt]], [[Resolution\|resolution]] 1.82Å' scene=''>		<StructureSection load='2cwt' size='340' side='right'caption='[[2cwt]], [[Resolution\|resolution]] 1.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cwt]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"achromobacter_globiformis"_(conn_1928)_bergey_et_al._1930 "achromobacter globiformis" (conn 1928) bergey et al. 1930]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CWT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2CWT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cwt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"achromobacter_globiformis"_(conn_1928)_bergey_et_al._1930 "achromobacter globiformis" (conn 1928) bergey et al. 1930]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CWT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPQ:5-(2-CARBOXY-2-AMINOETHYL)-2-HYDROXY-1,4-BENZOQUINONE'>TPQ</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPQ:5-(2-CARBOXY-2-AMINOETHYL)-2-HYDROXY-1,4-BENZOQUINONE'>TPQ</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1iu7\|1iu7]], [[2cwu\|2cwu]], [[2cwv\|2cwv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1iu7\|1iu7]], [[2cwu\|2cwu]], [[2cwv\|2cwv]]</div></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Oxidoreductase Oxidoreductase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.21 and 1.4.3.22 1.4.3.21 and 1.4.3.22] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Oxidoreductase Oxidoreductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.21 and 1.4.3.22 1.4.3.21 and 1.4.3.22] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2cwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cwt OCA], [~~http~~://pdbe.org/2cwt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2cwt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2cwt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2cwt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cwt OCA], [https://pdbe.org/2cwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cwt RCSB], [https://www.ebi.ac.uk/pdbsum/2cwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cwt ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	</div>		</div>
	<div class="pdbe-citations 2cwt" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2cwt" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Copper amine oxidase 3D structures\|Copper amine oxidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:07, 26 February 2020

2020-02-26T11:07:02Z

← Older revision		Revision as of 11:07, 26 February 2020
Line 1:		Line 1:

	==Catalytic base deletion in copper amine oxidase from arthrobacter globiformis==		==Catalytic base deletion in copper amine oxidase from arthrobacter globiformis==
	<StructureSection load='2cwt' size='340' side='right' caption='[[2cwt]], [[Resolution\|resolution]] 1.82Å' scene=''>		<StructureSection load='2cwt' size='340' side='right'caption='[[2cwt]], [[Resolution\|resolution]] 1.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cwt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"achromobacter_globiformis"_(conn_1928)_bergey_et_al._1930 "achromobacter globiformis" (conn 1928) bergey et al. 1930]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CWT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CWT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cwt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"achromobacter_globiformis"_(conn_1928)_bergey_et_al._1930 "achromobacter globiformis" (conn 1928) bergey et al. 1930]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CWT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CWT FirstGlance]. <br>
Line 33:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Chiu, Y C]]		[[Category: Chiu, Y C]]

OCA at 08:35, 23 May 2018

2018-05-23T08:35:27Z

← Older revision		Revision as of 08:35, 23 May 2018
Line 1:		Line 1:

	==Catalytic base deletion in copper amine oxidase from arthrobacter globiformis==		==Catalytic base deletion in copper amine oxidase from arthrobacter globiformis==
	<StructureSection load='2cwt' size='340' side='right' caption='[[2cwt]], [[Resolution\|resolution]] 1.82Å' scene=''>		<StructureSection load='2cwt' size='340' side='right' caption='[[2cwt]], [[Resolution\|resolution]] 1.82Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1iu7\|1iu7]], [[2cwu\|2cwu]], [[2cwv\|2cwv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1iu7\|1iu7]], [[2cwu\|2cwu]], [[2cwv\|2cwv]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Oxidoreductase Oxidoreductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.21 and 1.4.3.22 1.4.3.21 and 1.4.3.22] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Oxidoreductase Oxidoreductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.21 and 1.4.3.22 1.4.3.21 and 1.4.3.22] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cwt OCA], [http://pdbe.org/2cwt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cwt RCSB], [http://www.ebi.ac.uk/pdbsum/2cwt PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cwt OCA], [http://pdbe.org/2cwt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cwt RCSB], [http://www.ebi.ac.uk/pdbsum/2cwt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2cwt ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cw/2cwt_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cw/2cwt_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	[[Category: Amine oxidase]]		[[Category: Amine oxidase]]
	[[Category: Catalytic base]]		[[Category: Catalytic base]]
			[[Category: Copper]]
	[[Category: Topaquinone]]		[[Category: Topaquinone]]
	[[Category: Tpq]]		[[Category: Tpq]]

OCA at 15:21, 8 February 2016

2016-02-08T15:21:24Z

← Older revision		Revision as of 15:21, 8 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cwt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 12:54, 10 September 2015

2015-09-10T12:54:28Z

← Older revision		Revision as of 12:54, 10 September 2015
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	<StructureSection load='2cwt' size='340' side='right' caption='[[2cwt]], [[Resolution\|resolution]] 1.82Å' scene=''>		<StructureSection load='2cwt' size='340' side='right' caption='[[2cwt]], [[Resolution\|resolution]] 1.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cwt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Arthrobacter_globiformis Arthrobacter~~ globiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CWT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CWT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cwt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"achromobacter_globiformis"_(conn_1928)_bergey_et_al._1930 "achromobacter globiformis" (conn 1928) bergey et al. 1930]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CWT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CWT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPQ:5-(2-CARBOXY-2-AMINOETHYL)-2-HYDROXY-1,4-BENZOQUINONE'>TPQ</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPQ:5-(2-CARBOXY-2-AMINOETHYL)-2-HYDROXY-1,4-BENZOQUINONE'>TPQ</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1iu7\|1iu7]], [[2cwu\|2cwu]], [[2cwv\|2cwv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1iu7\|1iu7]], [[2cwu\|2cwu]], [[2cwv\|2cwv]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Oxidoreductase Oxidoreductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.21 and 1.4.3.22 1.4.3.21 and 1.4.3.22] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Oxidoreductase Oxidoreductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.21 and 1.4.3.22 1.4.3.21 and 1.4.3.22] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cwt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cwt RCSB], [http://www.ebi.ac.uk/pdbsum/2cwt PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cwt OCA], [http://pdbe.org/2cwt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cwt RCSB], [http://www.ebi.ac.uk/pdbsum/2cwt PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2cwt" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Arthrobacter globiformis]]~~
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Chiu, Y C]]		[[Category: Chiu, Y C]]

OCA at 18:21, 15 January 2015

2015-01-15T18:21:16Z

← Older revision		Revision as of 18:21, 15 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cwt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arthrobacter_globiformis Arthrobacter globiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CWT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CWT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cwt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arthrobacter_globiformis Arthrobacter globiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CWT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CWT FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPQ:5-(2-CARBOXY-2-AMINOETHYL)-2-HYDROXY-1,4-BENZOQUINONE'>TPQ</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPQ:5-(2-CARBOXY-2-AMINOETHYL)-2-HYDROXY-1,4-BENZOQUINONE'>TPQ</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1iu7\|1iu7]], [[2cwu\|2cwu]], [[2cwv\|2cwv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1iu7\|1iu7]], [[2cwu\|2cwu]], [[2cwv\|2cwv]]</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Oxidoreductase Oxidoreductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.21 and 1.4.3.22 1.4.3.21 and 1.4.3.22] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Oxidoreductase Oxidoreductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.21 and 1.4.3.22 1.4.3.21 and 1.4.3.22] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cwt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cwt RCSB], [http://www.ebi.ac.uk/pdbsum/2cwt PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cwt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cwt RCSB], [http://www.ebi.ac.uk/pdbsum/2cwt PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	[[Category: Arthrobacter globiformis]]		[[Category: Arthrobacter globiformis]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Chiu, Y C.]]		[[Category: Chiu, Y C]]
	[[Category: Hayashi, H.]]		[[Category: Hayashi, H]]
	[[Category: Hirota, S.]]		[[Category: Hirota, S]]
	[[Category: Kamiya, N.]]		[[Category: Kamiya, N]]
	[[Category: Kawano, Y.]]		[[Category: Kawano, Y]]
	[[Category: Kim, M.]]		[[Category: Kim, M]]
	[[Category: Kuroda, S.]]		[[Category: Kuroda, S]]
	[[Category: Murakawa, T.]]		[[Category: Murakawa, T]]
	[[Category: Okajima, T.]]		[[Category: Okajima, T]]
	[[Category: Taki, M.]]		[[Category: Taki, M]]
	[[Category: Tanizawa, K.]]		[[Category: Tanizawa, K]]
	[[Category: Uchida, M.]]		[[Category: Uchida, M]]
	[[Category: Yamaguchi, H.]]		[[Category: Yamaguchi, H]]
	[[Category: Yamamoto, Y.]]		[[Category: Yamamoto, Y]]
	[[Category: Amine oxidase]]		[[Category: Amine oxidase]]
	[[Category: Catalytic base]]		[[Category: Catalytic base]]
	~~[[Category: Oxidoreductase]]~~
	[[Category: Topaquinone]]		[[Category: Topaquinone]]
	[[Category: Tpq]]		[[Category: Tpq]]

OCA at 02:55, 30 September 2014

2014-09-30T02:55:13Z

← Older revision		Revision as of 02:55, 30 September 2014
Line 1:		Line 1:
	[[Image:~~2cwt~~.~~png~~\|~~left~~\|~~200px~~]]		==Catalytic base deletion in copper amine oxidase from arthrobacter globiformis==
			<StructureSection load='2cwt' size='340' side='right' caption='[[2cwt]], [[Resolution\|resolution]] 1.82Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2cwt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arthrobacter_globiformis Arthrobacter globiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CWT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CWT FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene><br>
			<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPQ:5-(2-CARBOXY-2-AMINOETHYL)-2-HYDROXY-1,4-BENZOQUINONE'>TPQ</scene></td></tr>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1iu7\|1iu7]], [[2cwu\|2cwu]], [[2cwv\|2cwv]]</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Oxidoreductase Oxidoreductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.21 and 1.4.3.22 1.4.3.21 and 1.4.3.22] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cwt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cwt RCSB], [http://www.ebi.ac.uk/pdbsum/2cwt PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cw/2cwt_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Copper amine oxidase contains a post-translationally generated quinone cofactor, topa quinone (TPQ), which mediates electron transfer from the amine substrate to molecular oxygen. The overall catalytic reaction is divided into the former reductive and the latter oxidative half-reactions based on the redox state of TPQ. In the reductive half-reaction, substrate amine reacts with the C5 carbonyl group of the oxidized TPQ, forming the substrate Schiff base (TPQ(ssb)), which is then converted to the product Schiff base (TPQ(psb)). During this step, an invariant Asp residue with an elevated pKa is presumed to serve as a general base accepting the alpha proton of the substrate. When Asp298, the putative active-site base in the recombinant enzyme from Arthrobacter globiformis, was mutated into Ala, the catalytic efficiency dropped to a level of about 10(6) orders of magnitude smaller than the wild-type (WT) enzyme, consistent with the essentiality of Asp298. Global analysis of the slow UV/vis spectral changes observed during the reductive half-reaction of the D298A mutant with 2-phenylethylamine provided apparent rate constants for the formation and decay of TPQ(ssb) (k(obs) = 4.7 and 4.8 x 10(-4) s(-1), respectively), both of which are markedly smaller than those of the WT enzyme determined by rapid-scan stopped-flow analysis (k(obs) = 699 and 411 s(-1), respectively). Thus, Asp298 plays important roles not only in the alpha-proton abstraction from TPQ(ssb) but also in other steps in the reductive half-reaction. X-ray diffraction analyses of D298A crystals soaked with the substrate for 1 h and 1 week revealed the structures of TPQ(ssb) and TPQ(psb), respectively, as pre-assigned by single-crystal microspectrophotometry. Consistent with the stereospecificity of alpha-proton abstraction, the pro-S alpha-proton of TPQ(ssb) to be abstracted is positioned nearly perpendicularly to the plane formed by the Schiff-base imine double bond conjugating with the quinone ring of TPQ, so that the orbitals of sigma and pi electrons maximally overlap in the conjugate system. More intriguingly, the pro-S alpha proton of the substrate is released stereospecifically even in the reaction catalyzed by the base-lacking D298A mutant. On the basis of these results, we propose that the stereospecificity of alpha-proton abstraction is primarily determined by the conformation of TPQ(ssb), rather than the relative geometry of TPQ and the catalytic base.

	~~{{STRUCTURE_2cwt\| PDB=2cwt \| SCENE= }}~~		Kinetic and structural studies on the catalytic role of the aspartic acid residue conserved in copper amine oxidase.,Chiu YC, Okajima T, Murakawa T, Uchida M, Taki M, Hirota S, Kim M, Yamaguchi H, Kawano Y, Kamiya N, Kuroda S, Hayashi H, Yamamoto Y, Tanizawa K Biochemistry. 2006 Apr 4;45(13):4105-20. PMID:16566584<ref>PMID:16566584</ref>

	~~===Catalytic base deletion in copper amine oxidase from arthrobacter globiformis===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_16566584}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2cwt]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Arthrobacter_globiformis Arthrobacter globiformis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2CWT OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:016566584<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Arthrobacter globiformis]]		[[Category: Arthrobacter globiformis]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA: Protected "2cwt" [edit=sysop:move=sysop]

2013-01-06T12:05:44Z

Protected "2cwt" [edit=sysop:move=sysop]

← Older revision	Revision as of 12:05, 6 January 2013
(No difference)

OCA at 12:05, 6 January 2013

2013-01-06T12:05:41Z

← Older revision		Revision as of 12:05, 6 January 2013
Line 1:		Line 1:
	~~{{Seed}}~~
	[[Image:2cwt.png\|left\|200px]]		[[Image:2cwt.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2cwt", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2cwt\| PDB=2cwt \| SCENE= }}		{{STRUCTURE_2cwt\| PDB=2cwt \| SCENE= }}

	===Catalytic base deletion in copper amine oxidase from arthrobacter globiformis===		===Catalytic base deletion in copper amine oxidase from arthrobacter globiformis===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_16566584}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 16566584 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_16566584}}		{{ABSTRACT_PUBMED_16566584}}

	==About this Structure==		==About this Structure==
	~~2CWT~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Arthrobacter_globiformis Arthrobacter globiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CWT OCA].		[[2cwt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arthrobacter_globiformis Arthrobacter globiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CWT OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~16566584~~</ref><references group="xtra"/>		<ref group="xtra">PMID:016566584</ref><references group="xtra"/>
	[[Category: Arthrobacter globiformis]]		[[Category: Arthrobacter globiformis]]
			[[Category: Oxidoreductase]]
	[[Category: Chiu, Y C.]]		[[Category: Chiu, Y C.]]
	[[Category: Hayashi, H.]]		[[Category: Hayashi, H.]]
Line 41:		Line 30:
	[[Category: Amine oxidase]]		[[Category: Amine oxidase]]
	[[Category: Catalytic base]]		[[Category: Catalytic base]]
	[[Category: ~~Copper~~]]		[[Category: Oxidoreductase]]
	[[Category: Topaquinone]]		[[Category: Topaquinone]]
	[[Category: Tpq]]		[[Category: Tpq]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 23:58:51 2009''~~