2cxt - Revision history

OCA at 13:44, 13 March 2024

2024-03-13T13:44:45Z

← Older revision		Revision as of 13:44, 13 March 2024
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	<StructureSection load='2cxt' size='340' side='right'caption='[[2cxt]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2cxt' size='340' side='right'caption='[[2cxt]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cxt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CXT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CXT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cxt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CXT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CXT FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=F6P:FRUCTOSE~~-~~6-PHOSPHATE'>F6P</scene>~~, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[2cvp\|2cvp]], [[2cxn\|2cxn]], [[2cxo\|2cxo]], [[2cxp\|2cxp]], [[2cxq\|2cxq]], [[2cxr\|2cxr]], [[2cxs\|2cxs]], [[2cxu\|2cxu]]~~</~~div~~><~~/td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F6P:FRUCTOSE-6-PHOSPHATE'>F6P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cxt OCA], [https://pdbe.org/2cxt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cxt RCSB], [https://www.ebi.ac.uk/pdbsum/2cxt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cxt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cxt OCA], [https://pdbe.org/2cxt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cxt RCSB], [https://www.ebi.ac.uk/pdbsum/2cxt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cxt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/G6PI_MOUSE G6PI_MOUSE]] Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.		[https://www.uniprot.org/uniprot/G6PI_MOUSE G6PI_MOUSE] Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cxt ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cxt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Autocrine motility factor (AMF), a tumor-secreted cytokine, stimulates cell migration in vitro and metastasis in vivo. AMF is identical to the extracellular cytokines neuroleukin and maturation factor and, interestingly, to the intracellular enzyme phosphoglucose isomerase. The cytokine activity of AMF is inhibited by carbohydrate phosphate compounds as they compete for AMF binding with the carbohydrate moiety of the AMF receptor (AMFR), which is a glycosylated seven transmembrane helix protein. Here, we report the first comprehensive high-resolution crystal structure analyses of the inhibitor-free form and the eight types of inhibitor (phosphate, erythrose 4-phosphate (E4P), arabinose 5-phosphate (A5P), sorbitol 6-phosphate (S6P), 6-phosphogluconic acid (6PGA), fructose 6-phosphate (F6P), glucose 6-phosphate (G6P), or mannose 6-phosphate (M6P)) complexes of mouse AMF (mAMF). We assayed the inhibitory activities of these inhibitors against the cytokine activity of mAMF. The inhibitory activities of the six-carbon sugars (G6P, F6P, M6P, and 6PGA) were found to be significantly higher than those of the four or five-carbon sugars (E4P or A5P). The inhibitory activities clearly depend on the length of the inhibitor molecules. A structural comparison revealed that a water-mediated hydrogen bond between one end of the inhibitor and a rigid portion of the protein surface in the shorter-chain inhibitor (E4P) complex is replaced by a direct hydrogen bond in the longer-chain inhibitor (6PGA) complex. Thus, to obtain a new compound with higher inhibitory activities against AMF, water molecules at the inhibitor binding site of AMF should be replaced by a functional group of inhibitors in order to introduce direct interactions with the protein surface. The present structure-activity relationship studies will be valuable not only for designing more effective AMF inhibitors but also for studying general protein-inhibitor interactions.

	Crystal structures of mouse autocrine motility factor in complex with carbohydrate phosphate inhibitors provide insight into structure-activity relationship of the inhibitors.,Tanaka N, Haga A, Naba N, Shiraiwa K, Kusakabe Y, Hashimoto K, Funasaka T, Nagase H, Raz A, Nakamura KT J Mol Biol. 2006 Feb 17;356(2):312-24. Epub 2005 Dec 9. PMID:16375918<ref>PMID:16375918</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2cxt" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Phosphoglucose isomerase 3D structures\|Phosphoglucose isomerase 3D structures]]		*[[Phosphoglucose isomerase 3D structures\|Phosphoglucose isomerase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Glucose-6-phosphate isomerase]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Lk3 transgenic mice~~]]		[[Category: Mus musculus]]
	[[Category: Funasaka, T]]		[[Category: Funasaka T]]
	[[Category: Haga, A]]		[[Category: Haga A]]
	[[Category: Hashimoto, K]]		[[Category: Hashimoto K]]
	[[Category: Kusakabe, Y]]		[[Category: Kusakabe Y]]
	[[Category: Naba, N]]		[[Category: Naba N]]
	[[Category: Nagase, H]]		[[Category: Nagase H]]
	[[Category: Nakamura~~, K T~~]]		[[Category: Nakamura KT]]
	[[Category: Raz, A]]		[[Category: Raz A]]
	[[Category: Shiraiwa, K]]		[[Category: Shiraiwa K]]
	[[Category: Tanaka, N]]		[[Category: Tanaka N]]
	~~[[Category: Isomerase~~]]

OCA at 10:23, 8 December 2021

2021-12-08T10:23:30Z

← Older revision		Revision as of 10:23, 8 December 2021
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	<StructureSection load='2cxt' size='340' side='right'caption='[[2cxt]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2cxt' size='340' side='right'caption='[[2cxt]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cxt]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CXT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2CXT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cxt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CXT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CXT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F6P:FRUCTOSE-6-PHOSPHATE'>F6P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F6P:FRUCTOSE-6-PHOSPHATE'>F6P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2cvp\|2cvp]], [[2cxn\|2cxn]], [[2cxo\|2cxo]], [[2cxp\|2cxp]], [[2cxq\|2cxq]], [[2cxr\|2cxr]], [[2cxs\|2cxs]], [[2cxu\|2cxu]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2cvp\|2cvp]], [[2cxn\|2cxn]], [[2cxo\|2cxo]], [[2cxp\|2cxp]], [[2cxq\|2cxq]], [[2cxr\|2cxr]], [[2cxs\|2cxs]], [[2cxu\|2cxu]]</div></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2cxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cxt OCA], [~~http~~://pdbe.org/2cxt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2cxt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2cxt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2cxt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cxt OCA], [https://pdbe.org/2cxt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cxt RCSB], [https://www.ebi.ac.uk/pdbsum/2cxt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cxt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/G6PI_MOUSE G6PI_MOUSE]] Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.		[[https://www.uniprot.org/uniprot/G6PI_MOUSE G6PI_MOUSE]] Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	==See Also==		==See Also==
	*[[Phosphoglucoisomerase\|Phosphoglucoisomerase]]
	*[[Phosphoglucose isomerase 3D structures\|Phosphoglucose isomerase 3D structures]]		*[[Phosphoglucose isomerase 3D structures\|Phosphoglucose isomerase 3D structures]]
	== References ==		== References ==

OCA at 07:47, 4 March 2020

2020-03-04T07:47:28Z

← Older revision		Revision as of 07:47, 4 March 2020
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	==Crystal structure of mouse AMF / F6P complex==		==Crystal structure of mouse AMF / F6P complex==
	<StructureSection load='2cxt' size='340' side='right' caption='[[2cxt]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2cxt' size='340' side='right'caption='[[2cxt]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cxt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CXT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cxt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CXT FirstGlance]. <br>
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	==See Also==		==See Also==
	*[[Phosphoglucoisomerase\|Phosphoglucoisomerase]]		*[[Phosphoglucoisomerase\|Phosphoglucoisomerase]]
	*[[Phosphoglucose isomerase\|Phosphoglucose isomerase]]		*[[Phosphoglucose isomerase 3D structures\|Phosphoglucose isomerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Glucose-6-phosphate isomerase]]		[[Category: Glucose-6-phosphate isomerase]]
			[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Funasaka, T]]		[[Category: Funasaka, T]]

OCA at 06:34, 30 May 2018

2018-05-30T06:34:30Z

← Older revision		Revision as of 06:34, 30 May 2018
Line 1:		Line 1:

	==Crystal structure of mouse AMF / F6P complex==		==Crystal structure of mouse AMF / F6P complex==
	<StructureSection load='2cxt' size='340' side='right' caption='[[2cxt]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2cxt' size='340' side='right' caption='[[2cxt]], [[Resolution\|resolution]] 1.50Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2cvp\|2cvp]], [[2cxn\|2cxn]], [[2cxo\|2cxo]], [[2cxp\|2cxp]], [[2cxq\|2cxq]], [[2cxr\|2cxr]], [[2cxs\|2cxs]], [[2cxu\|2cxu]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2cvp\|2cvp]], [[2cxn\|2cxn]], [[2cxo\|2cxo]], [[2cxp\|2cxp]], [[2cxq\|2cxq]], [[2cxr\|2cxr]], [[2cxs\|2cxs]], [[2cxu\|2cxu]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cxt OCA], [http://pdbe.org/2cxt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cxt RCSB], [http://www.ebi.ac.uk/pdbsum/2cxt PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cxt OCA], [http://pdbe.org/2cxt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cxt RCSB], [http://www.ebi.ac.uk/pdbsum/2cxt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2cxt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cx/2cxt_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cx/2cxt_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 08:03, 9 February 2016

2016-02-09T08:03:41Z

← Older revision		Revision as of 08:03, 9 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cxt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 20:15, 10 September 2015

2015-09-10T20:15:27Z

← Older revision		Revision as of 20:15, 10 September 2015
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	<StructureSection load='2cxt' size='340' side='right' caption='[[2cxt]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2cxt' size='340' side='right' caption='[[2cxt]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cxt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CXT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cxt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CXT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F6P:FRUCTOSE-6-PHOSPHATE'>F6P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F6P:FRUCTOSE-6-PHOSPHATE'>F6P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2cvp\|2cvp]], [[2cxn\|2cxn]], [[2cxo\|2cxo]], [[2cxp\|2cxp]], [[2cxq\|2cxq]], [[2cxr\|2cxr]], [[2cxs\|2cxs]], [[2cxu\|2cxu]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2cvp\|2cvp]], [[2cxn\|2cxn]], [[2cxo\|2cxo]], [[2cxp\|2cxp]], [[2cxq\|2cxq]], [[2cxr\|2cxr]], [[2cxs\|2cxs]], [[2cxu\|2cxu]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cxt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cxt RCSB], [http://www.ebi.ac.uk/pdbsum/2cxt PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cxt OCA], [http://pdbe.org/2cxt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2cxt RCSB], [http://www.ebi.ac.uk/pdbsum/2cxt PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2cxt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 37:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Glucose-6-phosphate isomerase]]		[[Category: Glucose-6-phosphate isomerase]]
	[[Category: ~~Mus musculus~~]]		[[Category: Lk3 transgenic mice]]
	[[Category: Funasaka, T]]		[[Category: Funasaka, T]]
	[[Category: Haga, A]]		[[Category: Haga, A]]

OCA at 05:34, 25 December 2014

2014-12-25T05:34:16Z

← Older revision		Revision as of 05:34, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2cxt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CXT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2cxt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CXT FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F6P:FRUCTOSE-6-PHOSPHATE'>F6P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F6P:FRUCTOSE-6-PHOSPHATE'>F6P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2cvp\|2cvp]], [[2cxn\|2cxn]], [[2cxo\|2cxo]], [[2cxp\|2cxp]], [[2cxq\|2cxq]], [[2cxr\|2cxr]], [[2cxs\|2cxs]], [[2cxu\|2cxu]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2cvp\|2cvp]], [[2cxn\|2cxn]], [[2cxo\|2cxo]], [[2cxp\|2cxp]], [[2cxq\|2cxq]], [[2cxr\|2cxr]], [[2cxs\|2cxs]], [[2cxu\|2cxu]]</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cxt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cxt RCSB], [http://www.ebi.ac.uk/pdbsum/2cxt PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cxt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cxt RCSB], [http://www.ebi.ac.uk/pdbsum/2cxt PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/G6PI_MOUSE G6PI_MOUSE]] Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 38:
	[[Category: Glucose-6-phosphate isomerase]]		[[Category: Glucose-6-phosphate isomerase]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Funasaka, T.]]		[[Category: Funasaka, T]]
	[[Category: Haga, A.]]		[[Category: Haga, A]]
	[[Category: Hashimoto, K.]]		[[Category: Hashimoto, K]]
	[[Category: Kusakabe, Y.]]		[[Category: Kusakabe, Y]]
	[[Category: Naba, N.]]		[[Category: Naba, N]]
	[[Category: Nagase, H.]]		[[Category: Nagase, H]]
	[[Category: Nakamura, K T.]]		[[Category: Nakamura, K T]]
	[[Category: Raz, A.]]		[[Category: Raz, A]]
	[[Category: Shiraiwa, K.]]		[[Category: Shiraiwa, K]]
	[[Category: Tanaka, N.]]		[[Category: Tanaka, N]]
	[[Category: Isomerase]]		[[Category: Isomerase]]

OCA at 01:07, 30 September 2014

2014-09-30T01:07:49Z

← Older revision		Revision as of 01:07, 30 September 2014
Line 1:		Line 1:
	~~{{STRUCTURE_2cxt~~\| ~~PDB~~=2cxt \| ~~SCENE~~= }}		==Crystal structure of mouse AMF / F6P complex==
	===~~Crystal~~ structure of ~~mouse AMF~~ / ~~F6P complex~~===		<StructureSection load='2cxt' size='340' side='right' caption='[[2cxt]], [[Resolution\|resolution]] 1.50Å' scene=''>
	~~{{ABSTRACT_PUBMED_16375918}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[2cxt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CXT FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F6P:FRUCTOSE-6-PHOSPHATE'>F6P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2cvp\|2cvp]], [[2cxn\|2cxn]], [[2cxo\|2cxo]], [[2cxp\|2cxp]], [[2cxq\|2cxq]], [[2cxr\|2cxr]], [[2cxs\|2cxs]], [[2cxu\|2cxu]]</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cxt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cxt RCSB], [http://www.ebi.ac.uk/pdbsum/2cxt PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cx/2cxt_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Autocrine motility factor (AMF), a tumor-secreted cytokine, stimulates cell migration in vitro and metastasis in vivo. AMF is identical to the extracellular cytokines neuroleukin and maturation factor and, interestingly, to the intracellular enzyme phosphoglucose isomerase. The cytokine activity of AMF is inhibited by carbohydrate phosphate compounds as they compete for AMF binding with the carbohydrate moiety of the AMF receptor (AMFR), which is a glycosylated seven transmembrane helix protein. Here, we report the first comprehensive high-resolution crystal structure analyses of the inhibitor-free form and the eight types of inhibitor (phosphate, erythrose 4-phosphate (E4P), arabinose 5-phosphate (A5P), sorbitol 6-phosphate (S6P), 6-phosphogluconic acid (6PGA), fructose 6-phosphate (F6P), glucose 6-phosphate (G6P), or mannose 6-phosphate (M6P)) complexes of mouse AMF (mAMF). We assayed the inhibitory activities of these inhibitors against the cytokine activity of mAMF. The inhibitory activities of the six-carbon sugars (G6P, F6P, M6P, and 6PGA) were found to be significantly higher than those of the four or five-carbon sugars (E4P or A5P). The inhibitory activities clearly depend on the length of the inhibitor molecules. A structural comparison revealed that a water-mediated hydrogen bond between one end of the inhibitor and a rigid portion of the protein surface in the shorter-chain inhibitor (E4P) complex is replaced by a direct hydrogen bond in the longer-chain inhibitor (6PGA) complex. Thus, to obtain a new compound with higher inhibitory activities against AMF, water molecules at the inhibitor binding site of AMF should be replaced by a functional group of inhibitors in order to introduce direct interactions with the protein surface. The present structure-activity relationship studies will be valuable not only for designing more effective AMF inhibitors but also for studying general protein-inhibitor interactions.

	~~==About this Structure==~~		Crystal structures of mouse autocrine motility factor in complex with carbohydrate phosphate inhibitors provide insight into structure-activity relationship of the inhibitors.,Tanaka N, Haga A, Naba N, Shiraiwa K, Kusakabe Y, Hashimoto K, Funasaka T, Nagase H, Raz A, Nakamura KT J Mol Biol. 2006 Feb 17;356(2):312-24. Epub 2005 Dec 9. PMID:16375918<ref>PMID:16375918</ref>
	~~[[2cxt]] is a~~ 2 ~~chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=2CXT OCA].~~
			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>

	==See Also==		==See Also==
	*[[Phosphoglucoisomerase\|Phosphoglucoisomerase]]		*[[Phosphoglucoisomerase\|Phosphoglucoisomerase]]
	*[[Phosphoglucose isomerase\|Phosphoglucose isomerase]]		*[[Phosphoglucose isomerase\|Phosphoglucose isomerase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:016375918<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Glucose-6-phosphate isomerase]]		[[Category: Glucose-6-phosphate isomerase]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]

OCA at 09:58, 11 March 2013

2013-03-11T09:58:06Z

← Older revision		Revision as of 09:58, 11 March 2013
Line 1:		Line 1:
	~~[[Image:2cxt.png\|left\|200px]]~~

	{{STRUCTURE_2cxt\| PDB=2cxt \| SCENE= }}		{{STRUCTURE_2cxt\| PDB=2cxt \| SCENE= }}

	===Crystal structure of mouse AMF / F6P complex===		===Crystal structure of mouse AMF / F6P complex===

	{{ABSTRACT_PUBMED_16375918}}		{{ABSTRACT_PUBMED_16375918}}

OCA at 13:12, 20 October 2012

2012-10-20T13:12:54Z

← Older revision		Revision as of 13:12, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[2cxt]] is a 2 chain structure ~~of [[Phosphoglucose isomerase]]~~ with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CXT OCA].		[[2cxt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CXT OCA].

	==See Also==		==See Also==