2d3a - Revision history

OCA at 13:46, 13 March 2024

2024-03-13T13:46:04Z

← Older revision		Revision as of 13:46, 13 March 2024
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	<StructureSection load='2d3a' size='340' side='right'caption='[[2d3a]], [[Resolution\|resolution]] 2.63Å' scene=''>		<StructureSection load='2d3a' size='340' side='right'caption='[[2d3a]], [[Resolution\|resolution]] 2.63Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2d3a]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Maize Maize~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D3A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D3A FirstGlance]. <br>		<table><tr><td colspan='2'>[[2d3a]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D3A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D3A FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ADP:ADENOSINE~~-~~5-DIPHOSPHATE'>ADP</scene>~~, ~~<scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=P3S:L-METHIONINE-S-SULFOXIMINE+PHOSPHATE'>P3S</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.63Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[2d3b\|2d3b]], [[2d3c\|2d3c]]~~</~~div~~><~~/td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=P3S:L-METHIONINE-S-SULFOXIMINE+PHOSPHATE'>P3S</scene></td></tr>
	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b></td><td class="sblockDat">gs1a~~ (~~[https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 MAIZE]~~)~~</td~~></tr>
	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glutamate~~--~~ammonia_ligase Glutamate~~-~~-ammonia ligase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d3a OCA], [https://pdbe.org/2d3a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d3a RCSB], [https://www.ebi.ac.uk/pdbsum/2d3a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d3a ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d3a OCA], [https://pdbe.org/2d3a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d3a RCSB], [https://www.ebi.ac.uk/pdbsum/2d3a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d3a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/GLNA3_MAIZE GLNA3_MAIZE]] Plays a role in the flow of nitrogen into nitrogenous organic compounds.		[https://www.uniprot.org/uniprot/GLNA3_MAIZE GLNA3_MAIZE] Plays a role in the flow of nitrogen into nitrogenous organic compounds.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d3a ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d3a ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Plants provide nourishment for animals and other heterotrophs as the sole primary producer in the food chain. Glutamine synthetase (GS), one of the essential enzymes for plant autotrophy catalyzes the incorporation of ammonia into glutamate to generate glutamine with concomitant hydrolysis of ATP, and plays a crucial role in the assimilation and re-assimilation of ammonia derived from a wide variety of metabolic processes during plant growth and development. Elucidation of the atomic structure of higher plant GS is important to understand its detailed reaction mechanism and to obtain further insight into plant productivity and agronomical utility. Here we report the first crystal structures of maize (Zea mays L.) GS. The structure reveals a unique decameric structure that differs significantly from the bacterial GS structure. Higher plants have several isoenzymes of GS differing in heat stability and catalytic properties for efficient responses to variation in the environment and nutrition. A key residue responsible for the heat stability was found to be Ile-161 in GS1a. The three structures in complex with substrate analogues, including phosphinothricin, a widely used herbicide, lead us to propose a mechanism for the transfer of phosphate from ATP to glutamate and to interpret the inhibitory action of phosphinothricin as a guide for the development of new potential herbicides.

	Atomic structure of plant glutamine synthetase: a key enzyme for plant productivity.,Unno H, Uchida T, Sugawara H, Kurisu G, Sugiyama T, Yamaya T, Sakakibara H, Hase T, Kusunoki M J Biol Chem. 2006 Sep 29;281(39):29287-96. Epub 2006 Jul 7. PMID:16829528<ref>PMID:16829528</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2d3a" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Glutamine synthetase 3D structures\|Glutamine synthetase 3D structures]]		*[[Glutamine synthetase 3D structures\|Glutamine synthetase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Glutamate--ammonia ligase]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Maize~~]]		[[Category: Zea mays]]
	[[Category: Hase, T]]		[[Category: Hase T]]
	[[Category: Kurisu, G]]		[[Category: Kurisu G]]
	[[Category: Kusunoki, M]]		[[Category: Kusunoki M]]
	[[Category: Sakakibara, H]]		[[Category: Sakakibara H]]
	[[Category: Sugawara, H]]		[[Category: Sugawara H]]
	[[Category: Sugiyama, T]]		[[Category: Sugiyama T]]
	[[Category: Uchida, T]]		[[Category: Uchida T]]
	[[Category: Unno, H]]		[[Category: Unno H]]
	[[Category: Yamaya, T]]		[[Category: Yamaya T]]
	~~[[Category: Glutamine synthetase maize]]~~
	~~[[Category: Ligase~~]]

OCA at 11:53, 3 February 2021

2021-02-03T11:53:48Z

← Older revision		Revision as of 11:53, 3 February 2021
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	==Crystal Structure of the Maize Glutamine Synthetase complexed with ADP and Methionine sulfoximine Phosphate==		==Crystal Structure of the Maize Glutamine Synthetase complexed with ADP and Methionine sulfoximine Phosphate==
	<StructureSection load='2d3a' size='340' side='right' caption='[[2d3a]], [[Resolution\|resolution]] 2.63Å' scene=''>		<StructureSection load='2d3a' size='340' side='right'caption='[[2d3a]], [[Resolution\|resolution]] 2.63Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2d3a]] is a 10 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Maize Maize]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D3A OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2D3A FirstGlance]. <br>		<table><tr><td colspan='2'>[[2d3a]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Maize Maize]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D3A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D3A FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=P3S:L-METHIONINE-S-SULFOXIMINE+PHOSPHATE'>P3S</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=P3S:L-METHIONINE-S-SULFOXIMINE+PHOSPHATE'>P3S</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2d3b\|2d3b]], [[2d3c\|2d3c]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2d3b\|2d3b]], [[2d3c\|2d3c]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gs1a ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 MAIZE])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gs1a ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 MAIZE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Glutamate--ammonia_ligase Glutamate--ammonia ligase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glutamate--ammonia_ligase Glutamate--ammonia ligase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2d3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d3a OCA], [~~http~~://pdbe.org/2d3a PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2d3a RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2d3a PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2d3a ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d3a OCA], [https://pdbe.org/2d3a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d3a RCSB], [https://www.ebi.ac.uk/pdbsum/2d3a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d3a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/GLNA3_MAIZE GLNA3_MAIZE]] Plays a role in the flow of nitrogen into nitrogenous organic compounds.		[[https://www.uniprot.org/uniprot/GLNA3_MAIZE GLNA3_MAIZE]] Plays a role in the flow of nitrogen into nitrogenous organic compounds.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	==See Also==		==See Also==
	*[[Glutamine synthetase\|Glutamine synthetase]]		*[[Glutamine synthetase 3D structures\|Glutamine synthetase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Glutamate--ammonia ligase]]		[[Category: Glutamate--ammonia ligase]]
			[[Category: Large Structures]]
	[[Category: Maize]]		[[Category: Maize]]
	[[Category: Hase, T]]		[[Category: Hase, T]]

OCA at 06:50, 30 May 2018

2018-05-30T06:50:19Z

← Older revision		Revision as of 06:50, 30 May 2018
Line 1:		Line 1:

	==Crystal Structure of the Maize Glutamine Synthetase complexed with ADP and Methionine sulfoximine Phosphate==		==Crystal Structure of the Maize Glutamine Synthetase complexed with ADP and Methionine sulfoximine Phosphate==
	<StructureSection load='2d3a' size='340' side='right' caption='[[2d3a]], [[Resolution\|resolution]] 2.63Å' scene=''>		<StructureSection load='2d3a' size='340' side='right' caption='[[2d3a]], [[Resolution\|resolution]] 2.63Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gs1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 MAIZE])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gs1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 MAIZE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate--ammonia_ligase Glutamate--ammonia ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate--ammonia_ligase Glutamate--ammonia ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d3a OCA], [http://pdbe.org/2d3a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2d3a RCSB], [http://www.ebi.ac.uk/pdbsum/2d3a PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d3a OCA], [http://pdbe.org/2d3a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2d3a RCSB], [http://www.ebi.ac.uk/pdbsum/2d3a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2d3a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 16:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d3/2d3a_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d3/2d3a_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d3a ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	==See Also==		==See Also==
	*[[Glutamine ~~Synthase~~\|Glutamine ~~Synthase~~]]		*[[Glutamine synthetase\|Glutamine synthetase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 22:37, 11 September 2015

2015-09-11T22:37:37Z

← Older revision		Revision as of 22:37, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2d3a' size='340' side='right' caption='[[2d3a]], [[Resolution\|resolution]] 2.63Å' scene=''>		<StructureSection load='2d3a' size='340' side='right' caption='[[2d3a]], [[Resolution\|resolution]] 2.63Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2d3a]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Zea_mays Zea mays~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D3A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D3A FirstGlance]. <br>		<table><tr><td colspan='2'>[[2d3a]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Maize Maize]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D3A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D3A FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=P3S:L-METHIONINE-S-SULFOXIMINE+PHOSPHATE'>P3S</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=P3S:L-METHIONINE-S-SULFOXIMINE+PHOSPHATE'>P3S</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2d3b\|2d3b]], [[2d3c\|2d3c]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2d3b\|2d3b]], [[2d3c\|2d3c]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gs1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 ~~Zea mays~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gs1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 MAIZE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate--ammonia_ligase Glutamate--ammonia ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate--ammonia_ligase Glutamate--ammonia ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d3a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2d3a RCSB], [http://www.ebi.ac.uk/pdbsum/2d3a PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d3a OCA], [http://pdbe.org/2d3a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2d3a RCSB], [http://www.ebi.ac.uk/pdbsum/2d3a PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 29:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2d3a" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 37:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Glutamate--ammonia ligase]]		[[Category: Glutamate--ammonia ligase]]
	[[Category: ~~Zea mays~~]]		[[Category: Maize]]
	[[Category: Hase, T]]		[[Category: Hase, T]]
	[[Category: Kurisu, G]]		[[Category: Kurisu, G]]

OCA at 20:22, 25 December 2014

2014-12-25T20:22:42Z

← Older revision		Revision as of 20:22, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2d3a]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D3A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D3A FirstGlance]. <br>		<table><tr><td colspan='2'>[[2d3a]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D3A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D3A FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=P3S:L-METHIONINE-S-SULFOXIMINE+PHOSPHATE'>P3S</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=P3S:L-METHIONINE-S-SULFOXIMINE+PHOSPHATE'>P3S</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2d3b\|2d3b]], [[2d3c\|2d3c]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2d3b\|2d3b]], [[2d3c\|2d3c]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gs1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 Zea mays])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gs1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 Zea mays])</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate--ammonia_ligase Glutamate--ammonia ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate--ammonia_ligase Glutamate--ammonia ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d3a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2d3a RCSB], [http://www.ebi.ac.uk/pdbsum/2d3a PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d3a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2d3a RCSB], [http://www.ebi.ac.uk/pdbsum/2d3a PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/GLNA3_MAIZE GLNA3_MAIZE]] Plays a role in the flow of nitrogen into nitrogenous organic compounds.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 38:
	[[Category: Glutamate--ammonia ligase]]		[[Category: Glutamate--ammonia ligase]]
	[[Category: Zea mays]]		[[Category: Zea mays]]
	[[Category: Hase, T.]]		[[Category: Hase, T]]
	[[Category: Kurisu, G.]]		[[Category: Kurisu, G]]
	[[Category: Kusunoki, M.]]		[[Category: Kusunoki, M]]
	[[Category: Sakakibara, H.]]		[[Category: Sakakibara, H]]
	[[Category: Sugawara, H.]]		[[Category: Sugawara, H]]
	[[Category: Sugiyama, T.]]		[[Category: Sugiyama, T]]
	[[Category: Uchida, T.]]		[[Category: Uchida, T]]
	[[Category: Unno, H.]]		[[Category: Unno, H]]
	[[Category: Yamaya, T.]]		[[Category: Yamaya, T]]
	[[Category: Glutamine synthetase maize]]		[[Category: Glutamine synthetase maize]]
	[[Category: Ligase]]		[[Category: Ligase]]

OCA at 03:45, 29 September 2014

2014-09-29T03:45:48Z

← Older revision		Revision as of 03:45, 29 September 2014
Line 1:		Line 1:
	[[Image:~~2d3a~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal Structure of the Maize Glutamine Synthetase complexed with ADP and Methionine sulfoximine Phosphate==
			<StructureSection load='2d3a' size='340' side='right' caption='[[2d3a]], [[Resolution\|resolution]] 2.63Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2d3a]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D3A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D3A FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=P3S:L-METHIONINE-S-SULFOXIMINE+PHOSPHATE'>P3S</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2d3b\|2d3b]], [[2d3c\|2d3c]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gs1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 Zea mays])</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate--ammonia_ligase Glutamate--ammonia ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d3a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2d3a RCSB], [http://www.ebi.ac.uk/pdbsum/2d3a PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d3/2d3a_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Plants provide nourishment for animals and other heterotrophs as the sole primary producer in the food chain. Glutamine synthetase (GS), one of the essential enzymes for plant autotrophy catalyzes the incorporation of ammonia into glutamate to generate glutamine with concomitant hydrolysis of ATP, and plays a crucial role in the assimilation and re-assimilation of ammonia derived from a wide variety of metabolic processes during plant growth and development. Elucidation of the atomic structure of higher plant GS is important to understand its detailed reaction mechanism and to obtain further insight into plant productivity and agronomical utility. Here we report the first crystal structures of maize (Zea mays L.) GS. The structure reveals a unique decameric structure that differs significantly from the bacterial GS structure. Higher plants have several isoenzymes of GS differing in heat stability and catalytic properties for efficient responses to variation in the environment and nutrition. A key residue responsible for the heat stability was found to be Ile-161 in GS1a. The three structures in complex with substrate analogues, including phosphinothricin, a widely used herbicide, lead us to propose a mechanism for the transfer of phosphate from ATP to glutamate and to interpret the inhibitory action of phosphinothricin as a guide for the development of new potential herbicides.

	~~{{STRUCTURE_2d3a\| PDB=2d3a \| SCENE= }}~~		Atomic structure of plant glutamine synthetase: a key enzyme for plant productivity.,Unno H, Uchida T, Sugawara H, Kurisu G, Sugiyama T, Yamaya T, Sakakibara H, Hase T, Kusunoki M J Biol Chem. 2006 Sep 29;281(39):29287-96. Epub 2006 Jul 7. PMID:16829528<ref>PMID:16829528</ref>

	~~===Crystal Structure~~ of the ~~Maize Glutamine Synthetase complexed with ADP and Methionine sulfoximine Phosphate===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_16829528}}~~

	~~==About this Structure==~~
	~~[[2d3a]] is a 10 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Zea_mays Zea mays]~~. ~~Full crystallographic information is available from [http://oca.weizmann.ac.il~~/~~oca-bin/ocashort?id=2D3A OCA].~~

	==See Also==		==See Also==
	*[[Glutamine Synthase\|Glutamine Synthase]]		*[[Glutamine Synthase\|Glutamine Synthase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:016829528<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Glutamate--ammonia ligase]]		[[Category: Glutamate--ammonia ligase]]
	[[Category: Zea mays]]		[[Category: Zea mays]]

OCA at 21:03, 20 October 2012

2012-10-20T21:03:41Z

← Older revision		Revision as of 21:03, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[2d3a]] is a 10 chain structure ~~of [[Glutamine Synthase]]~~ with sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D3A OCA].		[[2d3a]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D3A OCA].

	==See Also==		==See Also==

OCA: Protected "2d3a" [edit=sysop:move=sysop]

2012-07-27T12:28:47Z

Protected "2d3a" [edit=sysop:move=sysop]

← Older revision	Revision as of 12:28, 27 July 2012
(No difference)

OCA at 12:28, 27 July 2012

2012-07-27T12:28:46Z

← Older revision		Revision as of 12:28, 27 July 2012
Line 1:		Line 1:
	~~{{Seed}}~~
	[[Image:2d3a.png\|left\|200px]]		[[Image:2d3a.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2d3a", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2d3a\| PDB=2d3a \| SCENE= }}		{{STRUCTURE_2d3a\| PDB=2d3a \| SCENE= }}

	===Crystal Structure of the Maize Glutamine Synthetase complexed with ADP and Methionine sulfoximine Phosphate===		===Crystal Structure of the Maize Glutamine Synthetase complexed with ADP and Methionine sulfoximine Phosphate===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_16829528}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 16829528 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_16829528}}		{{ABSTRACT_PUBMED_16829528}}

	==About this Structure==		==About this Structure==
	~~2D3A~~ is a 10 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D3A OCA].		[[2d3a]] is a 10 chain structure of [[Glutamine Synthase]] with sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D3A OCA].

			==See Also==
			*[[Glutamine Synthase\|Glutamine Synthase]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~16829528~~</ref><references group="xtra"/>		<ref group="xtra">PMID:016829528</ref><references group="xtra"/>
	[[Category: Glutamate--ammonia ligase]]		[[Category: Glutamate--ammonia ligase]]
	[[Category: Zea mays]]		[[Category: Zea mays]]
Line 36:		Line 27:
	[[Category: Yamaya, T.]]		[[Category: Yamaya, T.]]
	[[Category: Glutamine synthetase maize]]		[[Category: Glutamine synthetase maize]]
			[[Category: Ligase]]
	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Feb 18 06:04:57 2009''~~

OCA at 04:04, 18 February 2009

2009-02-18T04:04:58Z

← Older revision		Revision as of 04:04, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
	2D3A is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D3A OCA].		2D3A is a 10 chains structure of sequences from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D3A OCA].

	==Reference==		==Reference==
	Atomic structure of plant glutamine synthetase: a key enzyme for plant productivity., Unno H, Uchida T, Sugawara H, Kurisu G, Sugiyama T, Yamaya T, Sakakibara H, Hase T, Kusunoki M, J Biol Chem. 2006 Sep 29;281(39):29287-96. Epub 2006 Jul 7. PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~16829528 16829528]~~		<ref group="xtra">PMID:16829528</ref><references group="xtra"/>
	[[Category: Glutamate--ammonia ligase]]		[[Category: Glutamate--ammonia ligase]]
	~~[[Category: Single protein]]~~
	[[Category: Zea mays]]		[[Category: Zea mays]]
	[[Category: Hase, T.]]		[[Category: Hase, T.]]
Line 38:		Line 37:
	[[Category: Glutamine synthetase maize]]		[[Category: Glutamine synthetase maize]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 12~~:48:~~10 2008~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 06:04:57 2009''