2dso - Revision history

OCA at 08:29, 25 October 2023

2023-10-25T08:29:29Z

← Older revision		Revision as of 08:29, 25 October 2023
Line 3:		Line 3:
	<StructureSection load='2dso' size='340' side='right'caption='[[2dso]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2dso' size='340' side='right'caption='[[2dso]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2dso]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus~~ aureus~~" (rosenbach 1884) zopf 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DSO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DSO FirstGlance]. <br>		<table><tr><td colspan='2'>[[2dso]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DSO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DSO FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[2dg0\|2dg0]], [[2dg1\|2dg1]]~~</~~div~~><~~/td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/1,4-lactonase 1,4-lactonase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.25 3.1.1.25]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2dso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dso OCA], [https://pdbe.org/2dso PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2dso RCSB], [https://www.ebi.ac.uk/pdbsum/2dso PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2dso ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2dso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dso OCA], [https://pdbe.org/2dso PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2dso RCSB], [https://www.ebi.ac.uk/pdbsum/2dso PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2dso ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/DRP35_STAAM DRP35_STAAM]] Exhibits lactonase activity. Acts in cells with perturbed membrane integrity and is possibly related to the membrane homeostasis (By similarity).		[https://www.uniprot.org/uniprot/DRP35_STAAM DRP35_STAAM] Exhibits lactonase activity. Acts in cells with perturbed membrane integrity and is possibly related to the membrane homeostasis (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: 1,4-lactonase]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Morikawa, K]]		[[Category: Staphylococcus aureus]]
	[[Category: Ohki, Y]]		[[Category: Morikawa K]]
	[[Category: Ohta, T]]		[[Category: Ohki Y]]
	[[Category: Tanaka, I]]		[[Category: Ohta T]]
	[[Category: Tanaka, Y]]		[[Category: Tanaka I]]
	[[Category: Watanabe, N]]		[[Category: Tanaka Y]]
	[[Category: Yao, M]]		[[Category: Watanabe N]]
	~~[[Category: Beta propeller]]~~		[[Category: Yao M]]
	~~[[Category: Hydrolase~~]]

OCA at 06:51, 10 November 2021

2021-11-10T06:51:10Z

← Older revision		Revision as of 06:51, 10 November 2021
Line 3:		Line 3:
	<StructureSection load='2dso' size='340' side='right'caption='[[2dso]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2dso' size='340' side='right'caption='[[2dso]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2dso]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DSO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2DSO FirstGlance]. <br>		<table><tr><td colspan='2'>[[2dso]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DSO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DSO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2dg0\|2dg0]], [[2dg1\|2dg1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2dg0\|2dg0]], [[2dg1\|2dg1]]</div></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/1,4-lactonase 1,4-lactonase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.25 3.1.1.25] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/1,4-lactonase 1,4-lactonase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.25 3.1.1.25] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2dso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dso OCA], [~~http~~://pdbe.org/2dso PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2dso RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2dso PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2dso ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2dso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dso OCA], [https://pdbe.org/2dso PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2dso RCSB], [https://www.ebi.ac.uk/pdbsum/2dso PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2dso ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/DRP35_STAAM DRP35_STAAM]] Exhibits lactonase activity. Acts in cells with perturbed membrane integrity and is possibly related to the membrane homeostasis (By similarity).		[[https://www.uniprot.org/uniprot/DRP35_STAAM DRP35_STAAM]] Exhibits lactonase activity. Acts in cells with perturbed membrane integrity and is possibly related to the membrane homeostasis (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:58, 4 March 2020

2020-03-04T07:58:34Z

← Older revision		Revision as of 07:58, 4 March 2020
Line 1:		Line 1:

	==Crystal structure of D138N mutant of Drp35, a 35kDa drug responsive protein from Staphylococcus aureus==		==Crystal structure of D138N mutant of Drp35, a 35kDa drug responsive protein from Staphylococcus aureus==
	<StructureSection load='2dso' size='340' side='right' caption='[[2dso]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2dso' size='340' side='right'caption='[[2dso]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2dso]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DSO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DSO FirstGlance]. <br>		<table><tr><td colspan='2'>[[2dso]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DSO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DSO FirstGlance]. <br>
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: 1,4-lactonase]]		[[Category: 1,4-lactonase]]
			[[Category: Large Structures]]
	[[Category: Morikawa, K]]		[[Category: Morikawa, K]]
	[[Category: Ohki, Y]]		[[Category: Ohki, Y]]

OCA at 06:52, 30 May 2018

2018-05-30T06:52:39Z

← Older revision		Revision as of 06:52, 30 May 2018
Line 1:		Line 1:

	==Crystal structure of D138N mutant of Drp35, a 35kDa drug responsive protein from Staphylococcus aureus==		==Crystal structure of D138N mutant of Drp35, a 35kDa drug responsive protein from Staphylococcus aureus==
	<StructureSection load='2dso' size='340' side='right' caption='[[2dso]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2dso' size='340' side='right' caption='[[2dso]], [[Resolution\|resolution]] 2.10Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2dg0\|2dg0]], [[2dg1\|2dg1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2dg0\|2dg0]], [[2dg1\|2dg1]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1,4-lactonase 1,4-lactonase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.25 3.1.1.25] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1,4-lactonase 1,4-lactonase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.25 3.1.1.25] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dso OCA], [http://pdbe.org/2dso PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2dso RCSB], [http://www.ebi.ac.uk/pdbsum/2dso PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dso OCA], [http://pdbe.org/2dso PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2dso RCSB], [http://www.ebi.ac.uk/pdbsum/2dso PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2dso ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ds/2dso_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ds/2dso_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2dso ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 01:07, 12 September 2015

2015-09-12T01:07:43Z

← Older revision		Revision as of 01:07, 12 September 2015
Line 2:		Line 2:
	<StructureSection load='2dso' size='340' side='right' caption='[[2dso]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2dso' size='340' side='right' caption='[[2dso]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2dso]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus Staphylococcus~~ aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DSO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DSO FirstGlance]. <br>		<table><tr><td colspan='2'>[[2dso]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DSO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DSO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2dg0\|2dg0]], [[2dg1\|2dg1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2dg0\|2dg0]], [[2dg1\|2dg1]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1,4-lactonase 1,4-lactonase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.25 3.1.1.25] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1,4-lactonase 1,4-lactonase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.25 3.1.1.25] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dso OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2dso RCSB], [http://www.ebi.ac.uk/pdbsum/2dso PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dso OCA], [http://pdbe.org/2dso PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2dso RCSB], [http://www.ebi.ac.uk/pdbsum/2dso PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/DRP35_STAAM DRP35_STAAM]] Exhibits lactonase activity. Acts in cells with perturbed membrane integrity and is possibly related to the membrane homeostasis (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 26:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2dso" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 31:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: 1,4-lactonase]]		[[Category: 1,4-lactonase]]
	~~[[Category: Staphylococcus aureus]]~~
	[[Category: Morikawa, K]]		[[Category: Morikawa, K]]
	[[Category: Ohki, Y]]		[[Category: Ohki, Y]]

OCA at 18:38, 15 January 2015

2015-01-15T18:38:22Z

← Older revision		Revision as of 18:38, 15 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2dso]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DSO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DSO FirstGlance]. <br>		<table><tr><td colspan='2'>[[2dso]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DSO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DSO FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2dg0\|2dg0]], [[2dg1\|2dg1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2dg0\|2dg0]], [[2dg1\|2dg1]]</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1,4-lactonase 1,4-lactonase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.25 3.1.1.25] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1,4-lactonase 1,4-lactonase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.25 3.1.1.25] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dso OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2dso RCSB], [http://www.ebi.ac.uk/pdbsum/2dso PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dso OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2dso RCSB], [http://www.ebi.ac.uk/pdbsum/2dso PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	[[Category: 1,4-lactonase]]		[[Category: 1,4-lactonase]]
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
	[[Category: Morikawa, K.]]		[[Category: Morikawa, K]]
	[[Category: Ohki, Y.]]		[[Category: Ohki, Y]]
	[[Category: Ohta, T.]]		[[Category: Ohta, T]]
	[[Category: Tanaka, I.]]		[[Category: Tanaka, I]]
	[[Category: Tanaka, Y.]]		[[Category: Tanaka, Y]]
	[[Category: Watanabe, N.]]		[[Category: Watanabe, N]]
	[[Category: Yao, M.]]		[[Category: Yao, M]]
	[[Category: Beta propeller]]		[[Category: Beta propeller]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 01:25, 30 September 2014

2014-09-30T01:25:20Z

← Older revision		Revision as of 01:25, 30 September 2014
Line 1:		Line 1:
	[[Image:~~2dso~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of D138N mutant of Drp35, a 35kDa drug responsive protein from Staphylococcus aureus==
			<StructureSection load='2dso' size='340' side='right' caption='[[2dso]], [[Resolution\|resolution]] 2.10Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2dso]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DSO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DSO FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2dg0\|2dg0]], [[2dg1\|2dg1]]</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1,4-lactonase 1,4-lactonase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.25 3.1.1.25] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dso OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2dso RCSB], [http://www.ebi.ac.uk/pdbsum/2dso PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ds/2dso_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Drp35 is a protein induced by cell wall-affecting antibiotics or detergents; it possesses calcium-dependent lactonase activity. To determine the molecular basis of the lactonase activity, we first solved the crystal structures of Drp35 with and without Ca(2+); these showed that the molecule has a six-bladed beta-propeller structure with two calcium ions bound at the center of the beta-propeller and surface region. Mutational analyses of evolutionarily conserved residues revealed that the central calcium-binding site is essential for the enzymatic activity of Drp35. Substitution of some other amino acid residues for the calcium-binding residues demonstrated the critical contributions of Glu(48), Asp(138), and Asp(236) to the enzymatic activity. Differential scanning calorimetric analysis revealed that the loss of activity of E48Q and D236N, but not D138N, was attributed to their inability to hold the calcium ion. Further structural analysis of the D138N mutant indicates that it lacks a water molecule bound to the calcium ion rather than the calcium ion itself. Based on these observations and structural information, a possible catalytic mechanism in which the calcium ion and its binding residues play direct roles was proposed for the lactonase activity of Drp35.

	~~{{STRUCTURE_2dso\| PDB=2dso \| SCENE= }}~~		Structural and mutational analyses of Drp35 from Staphylococcus aureus: a possible mechanism for its lactonase activity.,Tanaka Y, Morikawa K, Ohki Y, Yao M, Tsumoto K, Watanabe N, Ohta T, Tanaka I J Biol Chem. 2007 Feb 23;282(8):5770-80. Epub 2006 Dec 13. PMID:17166853<ref>PMID:17166853</ref>

	~~===Crystal structure of D138N mutant of Drp35~~, a ~~35kDa drug responsive protein from Staphylococcus aureus===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_17166853}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2dso]] is a 6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Staphylococcus_aureus Staphylococcus aureus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2DSO OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:017166853<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: 1,4-lactonase]]		[[Category: 1,4-lactonase]]
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]

OCA: Protected "2dso" [edit=sysop:move=sysop]

2013-01-06T10:40:16Z

Protected "2dso" [edit=sysop:move=sysop]

← Older revision	Revision as of 10:40, 6 January 2013
(No difference)

OCA at 10:40, 6 January 2013

2013-01-06T10:40:12Z

← Older revision		Revision as of 10:40, 6 January 2013
Line 1:		Line 1:
	~~{{Seed}}~~
	[[Image:2dso.png\|left\|200px]]		[[Image:2dso.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2dso", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2dso\| PDB=2dso \| SCENE= }}		{{STRUCTURE_2dso\| PDB=2dso \| SCENE= }}

	===Crystal structure of D138N mutant of Drp35, a 35kDa drug responsive protein from Staphylococcus aureus===		===Crystal structure of D138N mutant of Drp35, a 35kDa drug responsive protein from Staphylococcus aureus===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_17166853}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 17166853 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_17166853}}		{{ABSTRACT_PUBMED_17166853}}

	==About this Structure==		==About this Structure==
	~~2DSO~~ is a 6 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DSO OCA].		[[2dso]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DSO OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~17166853~~</ref><references group="xtra"/>		<ref group="xtra">PMID:017166853</ref><references group="xtra"/>
	[[Category: 1,4-lactonase]]		[[Category: 1,4-lactonase]]
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
Line 34:		Line 22:
	[[Category: Yao, M.]]		[[Category: Yao, M.]]
	[[Category: Beta propeller]]		[[Category: Beta propeller]]
			[[Category: Hydrolase]]
	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Feb 18 08:54:06 2009''~~

OCA at 06:54, 18 February 2009

2009-02-18T06:54:06Z

← Older revision		Revision as of 06:54, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
	2DSO is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DSO OCA].		2DSO is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DSO OCA].

	==Reference==		==Reference==
	Structural and mutational analyses of Drp35 from Staphylococcus aureus: a possible mechanism for its lactonase activity., Tanaka Y, Morikawa K, Ohki Y, Yao M, Tsumoto K, Watanabe N, Ohta T, Tanaka I, J Biol Chem. 2007 Feb 23;282(8):5770-80. Epub 2006 Dec 13. PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~17166853 17166853]~~		<ref group="xtra">PMID:17166853</ref><references group="xtra"/>
	[[Category: 1,4-lactonase]]		[[Category: 1,4-lactonase]]
	~~[[Category: Single protein]]~~
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
	[[Category: Morikawa, K.]]		[[Category: Morikawa, K.]]
Line 36:		Line 35:
	[[Category: Beta propeller]]		[[Category: Beta propeller]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 15~~:06~~:19 2008~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 08:54:06 2009''