2ea1 - Revision history

OCA at 05:11, 17 October 2024

2024-10-17T05:11:05Z

← Older revision		Revision as of 05:11, 17 October 2024
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	<StructureSection load='2ea1' size='340' side='right'caption='[[2ea1]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2ea1' size='340' side='right'caption='[[2ea1]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ea1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EA1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ea1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EA1 FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GPG:GUANYLYL~~-2,~~5-PHOSPHOGUANOSINE'>GPG</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks'>[https~~:~~//en.wikipedia.org/wiki/Enterobacter_ribonuclease Enterobacter ribonuclease]~~, ~~with EC number [https://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=3.1.27.6 3.1.27.6]~~ </~~span~~></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GPG:GUANYLYL-2,5-PHOSPHOGUANOSINE'>GPG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ea1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ea1 OCA], [https://pdbe.org/2ea1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ea1 RCSB], [https://www.ebi.ac.uk/pdbsum/2ea1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ea1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ea1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ea1 OCA], [https://pdbe.org/2ea1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ea1 RCSB], [https://www.ebi.ac.uk/pdbsum/2ea1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ea1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/RNI_ECOLI RNI_ECOLI]] One of the few RNases that cleaves the phosphodiester bond between any two nucleotide. Shows a preference for cytidylic or guanylic acid.		[https://www.uniprot.org/uniprot/RNI_ECOLI RNI_ECOLI] One of the few RNases that cleaves the phosphodiester bond between any two nucleotide. Shows a preference for cytidylic or guanylic acid.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ea/2ea1_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ea/2ea1_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/~~initialview01~~.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ea1 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ea1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	We have constructed a strain that overproduces ribonuclease I of Escherichia coli and we have purified large quantities of the enzyme. Data from fluorescence, CD, and DSC measurements showed that it was a very stable protein. The conformation energy determined from urea and guanidine hydrochloride denaturation experiments was 11.5 kcal mol(-1) at pH 7.5. Thermal denaturation studies indicated that it had a T(m) of 64 degrees C at pH 4.0. RNase I belongs to the RNase T2/S-RNase group of endoribonucleases, but near the amino terminus it has an unusually long hydrophilic segment. Part of this was removed in the deletion construct, RNase I Delta(26-38). We have obtained crystals of both RNase I and of an enzyme-G2'p5'G complex in the P2(1) space group and oligonucleotide complexes with both wild type and mutant enzymes. The current study lays the groundwork for extensive investigation into the structure, function, and physical properties of this widely distributed group of ribonucleases.

	Overexpression, biophysical characterization, and crystallization of ribonuclease I from Escherichia coli, a broad-specificity enzyme in the RNase T2 family.,Padmanabhan S, Zhou K, Chu CY, Lim RW, Lim LW Arch Biochem Biophys. 2001 Jun 1;390(1):42-50. PMID:11368513<ref>PMID:11368513</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2ea1" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]		*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bacillus~~ coli ~~migula 1895]]~~		[[Category: Escherichia coli]]
	~~[[Category: Enterobacter ribonuclease~~]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Lim~~, L W~~]]		[[Category: Lim LW]]
	[[Category: Lim~~, R W~~]]		[[Category: Lim RW]]
	[[Category: Padmanabhan, S]]		[[Category: Padmanabhan S]]
	[[Category: Pan, J]]		[[Category: Pan J]]
	[[Category: Zhou, K]]		[[Category: Zhou K]]
	~~[[Category: Hydrolase]]~~
	~~[[Category: Protein-gpg complex~~]]

OCA at 17:22, 15 December 2021

2021-12-15T17:22:33Z

← Older revision		Revision as of 17:22, 15 December 2021
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	<StructureSection load='2ea1' size='340' side='right'caption='[[2ea1]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2ea1' size='340' side='right'caption='[[2ea1]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ea1]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EA1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2EA1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ea1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EA1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GPG:GUANYLYL-2,5-PHOSPHOGUANOSINE'>GPG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GPG:GUANYLYL-2,5-PHOSPHOGUANOSINE'>GPG</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Enterobacter_ribonuclease Enterobacter ribonuclease], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.6 3.1.27.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Enterobacter_ribonuclease Enterobacter ribonuclease], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.6 3.1.27.6] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ea1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ea1 OCA], [~~http~~://pdbe.org/2ea1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ea1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ea1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ea1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ea1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ea1 OCA], [https://pdbe.org/2ea1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ea1 RCSB], [https://www.ebi.ac.uk/pdbsum/2ea1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ea1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/RNI_ECOLI RNI_ECOLI]] One of the few RNases that cleaves the phosphodiester bond between any two nucleotide. Shows a preference for cytidylic or guanylic acid.		[[https://www.uniprot.org/uniprot/RNI_ECOLI RNI_ECOLI]] One of the few RNases that cleaves the phosphodiester bond between any two nucleotide. Shows a preference for cytidylic or guanylic acid.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	==See Also==		==See Also==
	*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]		*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]
	*[[Temp\|Temp]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:30, 11 March 2020

2020-03-11T08:30:16Z

← Older revision		Revision as of 08:30, 11 March 2020
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	==Crystal structure of Ribonuclease I from Escherichia coli COMPLEXED WITH GUANYLYL-2(PRIME),5(PRIME)-GUANOSINE==		==Crystal structure of Ribonuclease I from Escherichia coli COMPLEXED WITH GUANYLYL-2(PRIME),5(PRIME)-GUANOSINE==
	<StructureSection load='2ea1' size='340' side='right' caption='[[2ea1]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2ea1' size='340' side='right'caption='[[2ea1]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ea1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EA1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EA1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ea1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EA1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EA1 FirstGlance]. <br>
Line 31:		Line 31:

	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]
	*[[Temp\|Temp]]		*[[Temp\|Temp]]
	== References ==		== References ==
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	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Enterobacter ribonuclease]]		[[Category: Enterobacter ribonuclease]]
			[[Category: Large Structures]]
	[[Category: Lim, L W]]		[[Category: Lim, L W]]
	[[Category: Lim, R W]]		[[Category: Lim, R W]]

OCA at 07:02, 6 June 2018

2018-06-06T07:02:56Z

← Older revision		Revision as of 07:02, 6 June 2018
Line 1:		Line 1:

	==Crystal structure of Ribonuclease I from Escherichia coli COMPLEXED WITH GUANYLYL-2(PRIME),5(PRIME)-GUANOSINE==		==Crystal structure of Ribonuclease I from Escherichia coli COMPLEXED WITH GUANYLYL-2(PRIME),5(PRIME)-GUANOSINE==
	<StructureSection load='2ea1' size='340' side='right' caption='[[2ea1]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2ea1' size='340' side='right' caption='[[2ea1]], [[Resolution\|resolution]] 1.80Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GPG:GUANYLYL-2,5-PHOSPHOGUANOSINE'>GPG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GPG:GUANYLYL-2,5-PHOSPHOGUANOSINE'>GPG</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Enterobacter_ribonuclease Enterobacter ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.6 3.1.27.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Enterobacter_ribonuclease Enterobacter ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.6 3.1.27.6] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ea1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ea1 OCA], [http://pdbe.org/2ea1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ea1 RCSB], [http://www.ebi.ac.uk/pdbsum/2ea1 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ea1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ea1 OCA], [http://pdbe.org/2ea1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ea1 RCSB], [http://www.ebi.ac.uk/pdbsum/2ea1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ea1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ea/2ea1_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ea/2ea1_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ea1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:09, 11 September 2015

2015-09-11T10:09:34Z

← Older revision		Revision as of 10:09, 11 September 2015
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	<StructureSection load='2ea1' size='340' side='right' caption='[[2ea1]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2ea1' size='340' side='right' caption='[[2ea1]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ea1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EA1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EA1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ea1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EA1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EA1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GPG:GUANYLYL-2,5-PHOSPHOGUANOSINE'>GPG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GPG:GUANYLYL-2,5-PHOSPHOGUANOSINE'>GPG</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Enterobacter_ribonuclease Enterobacter ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.6 3.1.27.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Enterobacter_ribonuclease Enterobacter ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.6 3.1.27.6] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ea1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ea1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ea1 RCSB], [http://www.ebi.ac.uk/pdbsum/2ea1 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ea1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ea1 OCA], [http://pdbe.org/2ea1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ea1 RCSB], [http://www.ebi.ac.uk/pdbsum/2ea1 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2ea1" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
	*[[Temp\|Temp]]		*[[Temp\|Temp]]
	*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Bacillus coli migula 1895]]
	[[Category: Enterobacter ribonuclease]]		[[Category: Enterobacter ribonuclease]]
	~~[[Category: Escherichia coli]]~~
	[[Category: Lim, L W]]		[[Category: Lim, L W]]
	[[Category: Lim, R W]]		[[Category: Lim, R W]]

OCA at 14:56, 25 December 2014

2014-12-25T14:56:40Z

← Older revision		Revision as of 14:56, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ea1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EA1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EA1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ea1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EA1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EA1 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GPG:GUANYLYL-2,5-PHOSPHOGUANOSINE'>GPG</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GPG:GUANYLYL-2,5-PHOSPHOGUANOSINE'>GPG</scene></td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Enterobacter_ribonuclease Enterobacter ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.6 3.1.27.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Enterobacter_ribonuclease Enterobacter ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.6 3.1.27.6] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ea1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ea1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ea1 RCSB], [http://www.ebi.ac.uk/pdbsum/2ea1 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ea1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ea1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ea1 RCSB], [http://www.ebi.ac.uk/pdbsum/2ea1 PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/RNI_ECOLI RNI_ECOLI]] One of the few RNases that cleaves the phosphodiester bond between any two nucleotide. Shows a preference for cytidylic or guanylic acid.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 30:
	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
			*[[Temp\|Temp]]
			*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 38:
	[[Category: Enterobacter ribonuclease]]		[[Category: Enterobacter ribonuclease]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Lim, L W.]]		[[Category: Lim, L W]]
	[[Category: Lim, R W.]]		[[Category: Lim, R W]]
	[[Category: Padmanabhan, S.]]		[[Category: Padmanabhan, S]]
	[[Category: Pan, J.]]		[[Category: Pan, J]]
	[[Category: Zhou, K.]]		[[Category: Zhou, K]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Protein-gpg complex]]		[[Category: Protein-gpg complex]]

OCA at 10:14, 29 September 2014

2014-09-29T10:14:08Z

← Older revision		Revision as of 10:14, 29 September 2014
Line 1:		Line 1:
	[[Image:~~2ea1~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of Ribonuclease I from Escherichia coli COMPLEXED WITH GUANYLYL-2(PRIME),5(PRIME)-GUANOSINE==
			<StructureSection load='2ea1' size='340' side='right' caption='[[2ea1]], [[Resolution\|resolution]] 1.80Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2ea1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EA1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EA1 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GPG:GUANYLYL-2,5-PHOSPHOGUANOSINE'>GPG</scene><br>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Enterobacter_ribonuclease Enterobacter ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.6 3.1.27.6] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ea1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ea1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ea1 RCSB], [http://www.ebi.ac.uk/pdbsum/2ea1 PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ea/2ea1_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			We have constructed a strain that overproduces ribonuclease I of Escherichia coli and we have purified large quantities of the enzyme. Data from fluorescence, CD, and DSC measurements showed that it was a very stable protein. The conformation energy determined from urea and guanidine hydrochloride denaturation experiments was 11.5 kcal mol(-1) at pH 7.5. Thermal denaturation studies indicated that it had a T(m) of 64 degrees C at pH 4.0. RNase I belongs to the RNase T2/S-RNase group of endoribonucleases, but near the amino terminus it has an unusually long hydrophilic segment. Part of this was removed in the deletion construct, RNase I Delta(26-38). We have obtained crystals of both RNase I and of an enzyme-G2'p5'G complex in the P2(1) space group and oligonucleotide complexes with both wild type and mutant enzymes. The current study lays the groundwork for extensive investigation into the structure, function, and physical properties of this widely distributed group of ribonucleases.

	~~{{STRUCTURE_2ea1\| PDB=2ea1 \| SCENE= }}~~		Overexpression, biophysical characterization, and crystallization of ribonuclease I from Escherichia coli, a broad-specificity enzyme in the RNase T2 family.,Padmanabhan S, Zhou K, Chu CY, Lim RW, Lim LW Arch Biochem Biophys. 2001 Jun 1;390(1):42-50. PMID:11368513<ref>PMID:11368513</ref>

	~~===Crystal structure of Ribonuclease I from Escherichia coli COMPLEXED WITH GUANYLYL-2(PRIME)~~,~~5(PRIME)-GUANOSINE===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_11368513}}~~

	~~==About this Structure==~~
	~~[[2ea1]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http://oca.weizmann.ac.il~~/~~oca-bin/ocashort?id=2EA1 OCA].~~

	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:011368513<~~/~~ref~~><~~ref group="xtra">PMID:002541256</ref><references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Enterobacter ribonuclease]]		[[Category: Enterobacter ribonuclease]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]

OCA at 18:04, 20 October 2012

2012-10-20T18:04:01Z

← Older revision		Revision as of 18:04, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[2ea1]] is a 1 chain structure ~~of [[Ribonuclease]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EA1 OCA].		[[2ea1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EA1 OCA].

	==See Also==		==See Also==

OCA at 02:52, 27 July 2012

2012-07-27T02:52:04Z

← Older revision		Revision as of 02:52, 27 July 2012
Line 1:		Line 1:
	[[Image:2ea1.png\|left\|200px]]		[[Image:2ea1.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2ea1", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2ea1\| PDB=2ea1 \| SCENE= }}		{{STRUCTURE_2ea1\| PDB=2ea1 \| SCENE= }}

	===Crystal structure of Ribonuclease I from Escherichia coli COMPLEXED WITH GUANYLYL-2(PRIME),5(PRIME)-GUANOSINE===		===Crystal structure of Ribonuclease I from Escherichia coli COMPLEXED WITH GUANYLYL-2(PRIME),5(PRIME)-GUANOSINE===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_11368513}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 11368513 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_11368513}}		{{ABSTRACT_PUBMED_11368513}}

	==About this Structure==		==About this Structure==
	[[2ea1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EA1 OCA].		[[2ea1]] is a 1 chain structure of [[Ribonuclease]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EA1 OCA].

			==See Also==
			*[[Ribonuclease\|Ribonuclease]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~11368513~~</ref><ref group="xtra">PMID:~~2541256~~</ref><references group="xtra"/>		<ref group="xtra">PMID:011368513</ref><ref group="xtra">PMID:002541256</ref><references group="xtra"/>
	[[Category: Enterobacter ribonuclease]]		[[Category: Enterobacter ribonuclease]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]

OCA: Protected "2ea1" [edit=sysop:move=sysop]

2011-03-15T00:11:28Z

Protected "2ea1" [edit=sysop:move=sysop]

← Older revision	Revision as of 00:11, 15 March 2011
(No difference)