2eas - Revision history

OCA: Redirecting to 4ycl

2015-04-01T12:57:22Z

Redirecting to 4ycl

← Older revision		Revision as of 12:57, 1 April 2015
Line 1:		Line 1:
	~~==Crystal structure of the SR CA2+-ATPASE with bound CPA==~~		#REDIRECT [[4ycl]] This PDB entry is obsolete and replaced by 4ycl
	~~<StructureSection load='2eas' size='340' side='right' caption='[[2eas]], [[Resolution\|resolution]] 3.40Å' scene=''>~~
	~~== Structural highlights ==~~
	<table><tr><td colspan='2'>[[2eas]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EAS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EAS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CZA:(6AR,11AS,11BR)-10-ACETYL-9-HYDROXY-7,7-DIMETHYL-2,6,6A,7,11A,11B-HEXAHYDRO-11H-PYRROLO[1,2 2,3]ISOINDOLO[4,5,6-CD]INDOL-11-ONE'>CZA</scene></td></tr>
	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>~~
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wpg\|1wpg]], [[2agv\|2agv]], [[2ear\|2ear]], [[2eat\|2eat]], [[2eau\|2eau]]</td></tr>~~
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8] </span></td></tr>
	~~<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eas FirstGlance],~~ [~~http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eas OCA],~~ [~~http://www.rcsb.org/pdb/explore.do?structureId=2eas RCSB], [http://www.ebi.ac.uk/pdbsum/2eas PDBsum]</span></td></tr>~~
	~~</table>~~
	~~== Function ==~~
	~~[[http://www.uniprot.org/uniprot/AT2A1_RABIT AT2A1_RABIT~~]] This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction (By similarity).
	~~== Evolutionary Conservation ==~~
	~~[[Image:Consurf_key_small.gif\|200px\|right]]~~
	~~Check<jmol>~~
	~~<jmolCheckbox>~~
	~~<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ea/2eas_consurf.spt"</scriptWhenChecked>~~
	~~<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>~~
	~~<text>to colour the structure by Evolutionary Conservation</text>~~
	~~</jmolCheckbox>~~
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
	~~<div style="clear:both"></div>~~
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Ca(2+)-ATPase of skeletal muscle sarcoplasmic reticulum is an ATP-driven Ca(2+) pump consisting of three cytoplasmic domains and 10 transmembrane helices. In the absence of Ca(2+), the three cytoplasmic domains gather to form a compact headpiece, but the ATPase is unstable without an inhibitor. Here we describe the crystal structures of Ca(2+)-ATPase in the absence of Ca(2+) stabilized with cyclopiazonic acid alone and in combination with other inhibitors. Cyclopiazonic acid is located in the transmembrane region of the protein near the cytoplasmic surface. The binding site partially overlaps with that of 2,5-di-tert-butyl-1,4-dihydroxybenzene but is separate from that of thapsigargin. The overall structure is ~~significantly different from that stabilized with thapsigargin: The cytoplasmic headpiece is more upright,~~ and the transmembrane helices M1-M4 are rearranged. Cyclopiazonic acid primarily alters the position of the M1' helix and thereby M2 and M4 and then M5. Because M5 is integrated into the phosphorylation domain, the whole cytoplasmic headpiece moves. These structural changes show how an event in the transmembrane domain can be transmitted to the cytoplasmic domain despite flexible links between them. They also reveal that Ca(2+)-ATPase has considerable plasticity even when fixed by ~~a transmembrane inhibitor, presumably to accommodate thermal fluctuations.~~

	Interdomain communication in calcium pump as revealed in the crystal structures with transmembrane inhibitors.,Takahashi M, Kondou Y, Toyoshima C Proc Natl Acad Sci U S A. 2007 Apr 3;104(14):5800-5. Epub 2007 Mar 26. PMID:17389383<ref>PMID:17389383</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~

	~~==See Also==~~
	*[[ATPase\|ATPase]]
	~~== References ==~~
	~~<references/>~~
	~~__TOC__~~
	~~</StructureSection>~~
	~~[[Category: Calcium-transporting ATPase]]~~
	~~[[Category: Oryctolagus cuniculus]]~~
	~~[[Category: Kondou, Y]]~~
	~~[[Category: Takahashi, M]]~~
	~~[[Category: Toyoshima, C]]~~
	~~[[Category: Ca2+]]~~
	~~[[Category: Had fold]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: Ion pump]]~~
	~~[[Category: Membrane protein]]~~
	~~[[Category: P-type atpase]]~~

OCA at 20:45, 25 December 2014

2014-12-25T20:45:10Z

← Older revision		Revision as of 20:45, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2eas]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EAS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EAS FirstGlance]. <br>		<table><tr><td colspan='2'>[[2eas]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EAS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EAS FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CZA:(6AR,11AS,11BR)-10-ACETYL-9-HYDROXY-7,7-DIMETHYL-2,6,6A,7,11A,11B-HEXAHYDRO-11H-PYRROLO[1,2 2,3]ISOINDOLO[4,5,6-CD]INDOL-11-ONE'>CZA</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CZA:(6AR,11AS,11BR)-10-ACETYL-9-HYDROXY-7,7-DIMETHYL-2,6,6A,7,11A,11B-HEXAHYDRO-11H-PYRROLO[1,2 2,3]ISOINDOLO[4,5,6-CD]INDOL-11-ONE'>CZA</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wpg\|1wpg]], [[2agv\|2agv]], [[2ear\|2ear]], [[2eat\|2eat]], [[2eau\|2eau]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wpg\|1wpg]], [[2agv\|2agv]], [[2ear\|2ear]], [[2eat\|2eat]], [[2eau\|2eau]]</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eas OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2eas RCSB], [http://www.ebi.ac.uk/pdbsum/2eas PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eas OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2eas RCSB], [http://www.ebi.ac.uk/pdbsum/2eas PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/AT2A1_RABIT AT2A1_RABIT]] This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 38:
	[[Category: Calcium-transporting ATPase]]		[[Category: Calcium-transporting ATPase]]
	[[Category: Oryctolagus cuniculus]]		[[Category: Oryctolagus cuniculus]]
	[[Category: Kondou, Y.]]		[[Category: Kondou, Y]]
	[[Category: Takahashi, M.]]		[[Category: Takahashi, M]]
	[[Category: Toyoshima, C.]]		[[Category: Toyoshima, C]]
	[[Category: Ca2+]]		[[Category: Ca2+]]
	[[Category: Had fold]]		[[Category: Had fold]]

OCA at 04:10, 29 September 2014

2014-09-29T04:10:52Z

← Older revision		Revision as of 04:10, 29 September 2014
Line 1:		Line 1:
	[[Image:~~2eas~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of the SR CA2+-ATPASE with bound CPA==
			<StructureSection load='2eas' size='340' side='right' caption='[[2eas]], [[Resolution\|resolution]] 3.40Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2eas]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EAS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EAS FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CZA:(6AR,11AS,11BR)-10-ACETYL-9-HYDROXY-7,7-DIMETHYL-2,6,6A,7,11A,11B-HEXAHYDRO-11H-PYRROLO[1,2 2,3]ISOINDOLO[4,5,6-CD]INDOL-11-ONE'>CZA</scene><br>
			<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wpg\|1wpg]], [[2agv\|2agv]], [[2ear\|2ear]], [[2eat\|2eat]], [[2eau\|2eau]]</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eas OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2eas RCSB], [http://www.ebi.ac.uk/pdbsum/2eas PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ea/2eas_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Ca(2+)-ATPase of skeletal muscle sarcoplasmic reticulum is an ATP-driven Ca(2+) pump consisting of three cytoplasmic domains and 10 transmembrane helices. In the absence of Ca(2+), the three cytoplasmic domains gather to form a compact headpiece, but the ATPase is unstable without an inhibitor. Here we describe the crystal structures of Ca(2+)-ATPase in the absence of Ca(2+) stabilized with cyclopiazonic acid alone and in combination with other inhibitors. Cyclopiazonic acid is located in the transmembrane region of the protein near the cytoplasmic surface. The binding site partially overlaps with that of 2,5-di-tert-butyl-1,4-dihydroxybenzene but is separate from that of thapsigargin. The overall structure is significantly different from that stabilized with thapsigargin: The cytoplasmic headpiece is more upright, and the transmembrane helices M1-M4 are rearranged. Cyclopiazonic acid primarily alters the position of the M1' helix and thereby M2 and M4 and then M5. Because M5 is integrated into the phosphorylation domain, the whole cytoplasmic headpiece moves. These structural changes show how an event in the transmembrane domain can be transmitted to the cytoplasmic domain despite flexible links between them. They also reveal that Ca(2+)-ATPase has considerable plasticity even when fixed by a transmembrane inhibitor, presumably to accommodate thermal fluctuations.

	~~{{STRUCTURE_2eas\| PDB=2eas \| SCENE= }}~~		Interdomain communication in calcium pump as revealed in the crystal structures with transmembrane inhibitors.,Takahashi M, Kondou Y, Toyoshima C Proc Natl Acad Sci U S A. 2007 Apr 3;104(14):5800-5. Epub 2007 Mar 26. PMID:17389383<ref>PMID:17389383</ref>

	~~===Crystal structure~~ of the ~~SR CA2+-ATPASE with bound CPA===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_17389383}}~~

	~~==About this Structure==~~
	~~[[2eas]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]~~. ~~Full crystallographic information is available from [http://oca.weizmann.ac.il~~/~~oca-bin/ocashort?id=2EAS OCA].~~

	==See Also==		==See Also==
	*[[ATPase\|ATPase]]		*[[ATPase\|ATPase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:017389383<~~/~~ref~~><~~ref group="xtra">PMID:010864315</ref><ref group="xtra">PMID:012167852</ref><ref group="xtra">PMID:015229613</ref><ref group="xtra">PMID:015448704</ref><references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Calcium-transporting ATPase]]		[[Category: Calcium-transporting ATPase]]
	[[Category: Oryctolagus cuniculus]]		[[Category: Oryctolagus cuniculus]]

OCA at 21:25, 20 October 2012

2012-10-20T21:25:20Z

← Older revision		Revision as of 21:25, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[2eas]] is a 1 chain structure ~~of [[ATPase]]~~ with sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EAS OCA].		[[2eas]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EAS OCA].

	==See Also==		==See Also==
Line 14:		Line 14:

	==Reference==		==Reference==
	<ref group="xtra">PMID:017389383</ref><ref group="xtra">PMID:010864315</ref><ref group="xtra">PMID:012167852</ref><ref group="xtra">PMID:015229613</ref><ref group="xtra">PMID:015448704~~</ref><ref group="xtra">PMID:016150713~~</ref><references group="xtra"/>		<ref group="xtra">PMID:017389383</ref><ref group="xtra">PMID:010864315</ref><ref group="xtra">PMID:012167852</ref><ref group="xtra">PMID:015229613</ref><ref group="xtra">PMID:015448704</ref><references group="xtra"/>
	[[Category: Calcium-transporting ATPase]]		[[Category: Calcium-transporting ATPase]]
	[[Category: Oryctolagus cuniculus]]		[[Category: Oryctolagus cuniculus]]

OCA at 13:12, 27 July 2012

2012-07-27T13:12:22Z

← Older revision		Revision as of 13:12, 27 July 2012
Line 1:		Line 1:
	[[Image:2eas.png\|left\|200px]]		[[Image:2eas.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2eas", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2eas\| PDB=2eas \| SCENE= }}		{{STRUCTURE_2eas\| PDB=2eas \| SCENE= }}

	===Crystal structure of the SR CA2+-ATPASE with bound CPA===		===Crystal structure of the SR CA2+-ATPASE with bound CPA===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_17389383}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 17389383 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_17389383}}		{{ABSTRACT_PUBMED_17389383}}

Line 22:		Line 11:

	==See Also==		==See Also==
	*[[ATPase]]		*[[ATPase\|ATPase]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~17389383~~</ref><ref group="xtra">PMID:~~10864315~~</ref><ref group="xtra">PMID:~~12167852~~</ref><ref group="xtra">PMID:~~15229613~~</ref><ref group="xtra">PMID:~~15448704~~</ref><ref group="xtra">PMID:~~16150713~~</ref><references group="xtra"/>		<ref group="xtra">PMID:017389383</ref><ref group="xtra">PMID:010864315</ref><ref group="xtra">PMID:012167852</ref><ref group="xtra">PMID:015229613</ref><ref group="xtra">PMID:015448704</ref><ref group="xtra">PMID:016150713</ref><references group="xtra"/>
	[[Category: Calcium-transporting ATPase]]		[[Category: Calcium-transporting ATPase]]
	[[Category: Oryctolagus cuniculus]]		[[Category: Oryctolagus cuniculus]]

OCA: Protected "2eas" [edit=sysop:move=sysop]

2010-12-26T23:09:04Z

Protected "2eas" [edit=sysop:move=sysop]

← Older revision	Revision as of 23:09, 26 December 2010
(No difference)

OCA at 23:09, 26 December 2010

2010-12-26T23:09:02Z

← Older revision		Revision as of 23:09, 26 December 2010
Line 1:		Line 1:
	~~{{Seed}}~~
	[[Image:2eas.png\|left\|200px]]		[[Image:2eas.png\|left\|200px]]

Line 20:		Line 19:

	==About this Structure==		==About this Structure==
	~~2EAS~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EAS OCA].		[[2eas]] is a 1 chain structure of [[ATPase]] with sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EAS OCA].

			==See Also==
			*[[ATPase]]

	==Reference==		==Reference==
Line 35:		Line 37:
	[[Category: Membrane protein]]		[[Category: Membrane protein]]
	[[Category: P-type atpase]]		[[Category: P-type atpase]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 06:41:29 2009''~~

OCA at 04:41, 18 February 2009

2009-02-18T04:41:30Z

← Older revision		Revision as of 04:41, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
	2EAS is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EAS OCA].		2EAS is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EAS OCA].

	==Reference==		==Reference==
	Interdomain communication in calcium pump as revealed in the crystal structures with transmembrane inhibitors., Takahashi M, Kondou Y, Toyoshima C, Proc Natl Acad Sci U S A. 2007 Apr 3;104(14):5800-5. Epub 2007 Mar 26. PMID:~~[http://www.ncbi.nlm.nih.gov/pubmed~~/~~17389383 17389383]~~		<ref group="xtra">PMID:17389383</ref><ref group="xtra">PMID:10864315</ref><ref group="xtra">PMID:12167852</ref><ref group="xtra">PMID:15229613</ref><ref group="xtra">PMID:15448704</ref><ref group="xtra">PMID:16150713</ref><references group="xtra"/>

	~~Crystal structure of the calcium pump of sarcoplasmic reticulum at 2.6 A resolution., Toyoshima C, Nakasako M, Nomura H, Ogawa H, Nature. 2000 Jun 8;405(6787):647-55.~~ PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed/10864315 10864315]~~

	~~Structural changes in the calcium pump accompanying the dissociation of calcium., Toyoshima C, Nomura H, Nature. 2002 Aug 8;418(6898):605-11.~~ PMID:~~[http://www.ncbi.nlm.nih.gov/pubmed~~/~~12167852 12167852]~~

	~~Crystal structure of the calcium pump with a bound ATP analogue., Toyoshima C, Mizutani T, Nature. 2004 Jul 29;430(6999):529-35. Epub 2004 Jun 30.~~ PMID:~~[http://www.ncbi.nlm.nih.gov/pubmed~~/~~15229613 15229613]~~

	~~Lumenal gating mechanism revealed in calcium pump crystal structures with phosphate analogues., Toyoshima C, Nomura H, Tsuda T, Nature. 2004 Nov 18;432(7015):361-8. Epub 2004 Sep 26.~~ PMID:~~[http:~~/~~/www.ncbi.nlm.nih.gov/pubmed/15448704 15448704]~~

	Structural role of countertransport revealed in Ca(2+) pump crystal structure in the absence of Ca(2+)., Obara K, Miyashita N, Xu C, Toyoshima I, Sugita Y, Inesi G, Toyoshima C, Proc Natl Acad Sci U S A. 2005 Oct 11;102(41):14489-96. Epub 2005 Sep 6. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/16150713 16150713]~~
	[[Category: Calcium-transporting ATPase]]		[[Category: Calcium-transporting ATPase]]
	[[Category: Oryctolagus cuniculus]]		[[Category: Oryctolagus cuniculus]]
	~~[[Category: Single protein]]~~
	[[Category: Kondou, Y.]]		[[Category: Kondou, Y.]]
	[[Category: Takahashi, M.]]		[[Category: Takahashi, M.]]
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	[[Category: P-type atpase]]		[[Category: P-type atpase]]

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OCA at 10:25, 29 July 2008

2008-07-29T10:25:06Z

← Older revision		Revision as of 10:25, 29 July 2008
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			[[Image:2eas.png\|left\|200px]]

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	{{STRUCTURE_2eas\| PDB=2eas \| SCENE= }}		{{STRUCTURE_2eas\| PDB=2eas \| SCENE= }}

	~~'''~~Crystal structure of the SR CA2+-ATPASE with bound CPA~~'''~~		===Crystal structure of the SR CA2+-ATPASE with bound CPA===


	~~==Overview==~~		<!--
	~~Ca(2+)~~-~~ATPase of skeletal muscle sarcoplasmic reticulum is an ATP~~-~~driven Ca(2+) pump consisting of three cytoplasmic domains and 10 transmembrane helices. In the absence of Ca(2+)~~, the ~~three cytoplasmic domains gather~~ to ~~form a compact headpiece, but~~ the ~~ATPase is unstable without an inhibitor. Here we describe the crystal structures of Ca~~(~~2+)-ATPase in the absence of Ca(2+) stabilized with cyclopiazonic acid alone and in combination with other inhibitors~~. ~~Cyclopiazonic acid is located in the transmembrane region of the protein near the cytoplasmic surface~~. ~~The binding site partially overlaps with that of 2,5-di-tert-butyl-1~~,~~4-dihydroxybenzene but~~ is ~~separate from that of thapsigargin~~. ~~The overall structure is significantly different from that stabilized with thapsigargin: The cytoplasmic headpiece is more upright, and the transmembrane helices M1~~-M4 are rearranged. Cyclopiazonic acid primarily alters the position of the M1' helix and thereby M2 and M4 and then M5. Because M5 is integrated into the phosphorylation domain, the whole cytoplasmic headpiece moves. These structural changes show how an event in the transmembrane domain can be transmitted to the cytoplasmic domain despite flexible links between them. They also reveal that Ca(2+)-~~ATPase has considerable plasticity even when fixed by a transmembrane inhibitor, presumably to accommodate thermal fluctuations.~~		The line below this paragraph, {{ABSTRACT_PUBMED_17389383}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 17389383 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_17389383}}

	==About this Structure==		==About this Structure==
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	==Reference==		==Reference==
	Interdomain communication in calcium pump as revealed in the crystal structures with transmembrane inhibitors., Takahashi M, Kondou Y, Toyoshima C, Proc Natl Acad Sci U S A. 2007 Apr 3;104(14):5800-5. Epub 2007 Mar 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17389383 17389383]		Interdomain communication in calcium pump as revealed in the crystal structures with transmembrane inhibitors., Takahashi M, Kondou Y, Toyoshima C, Proc Natl Acad Sci U S A. 2007 Apr 3;104(14):5800-5. Epub 2007 Mar 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17389383 17389383]

			Crystal structure of the calcium pump of sarcoplasmic reticulum at 2.6 A resolution., Toyoshima C, Nakasako M, Nomura H, Ogawa H, Nature. 2000 Jun 8;405(6787):647-55. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10864315 10864315]

			Structural changes in the calcium pump accompanying the dissociation of calcium., Toyoshima C, Nomura H, Nature. 2002 Aug 8;418(6898):605-11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12167852 12167852]

			Crystal structure of the calcium pump with a bound ATP analogue., Toyoshima C, Mizutani T, Nature. 2004 Jul 29;430(6999):529-35. Epub 2004 Jun 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15229613 15229613]

			Lumenal gating mechanism revealed in calcium pump crystal structures with phosphate analogues., Toyoshima C, Nomura H, Tsuda T, Nature. 2004 Nov 18;432(7015):361-8. Epub 2004 Sep 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15448704 15448704]

			Structural role of countertransport revealed in Ca(2+) pump crystal structure in the absence of Ca(2+)., Obara K, Miyashita N, Xu C, Toyoshima I, Sugita Y, Inesi G, Toyoshima C, Proc Natl Acad Sci U S A. 2005 Oct 11;102(41):14489-96. Epub 2005 Sep 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16150713 16150713]
	[[Category: Calcium-transporting ATPase]]		[[Category: Calcium-transporting ATPase]]
	[[Category: Oryctolagus cuniculus]]		[[Category: Oryctolagus cuniculus]]
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	[[Category: Membrane protein]]		[[Category: Membrane protein]]
	[[Category: P-type atpase]]		[[Category: P-type atpase]]
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			''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:25:05 2008''

OCA at 10:24, 30 April 2008

2008-04-30T10:24:12Z

← Older revision		Revision as of 10:24, 30 April 2008
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	[[Image:2eas.gif\|left\|200px]]		[[Image:2eas.gif\|left\|200px]]

	~~{{Structure~~		<!--
	~~\|PDB= 2eas \|SIZE=350\|CAPTION= <scene name='initialview01'>2eas</scene>~~, ~~resolution 3~~.~~40Å~~		The line below this paragraph, containing "STRUCTURE_2eas", creates the "Structure Box" on the page.
	~~\|SITE=~~		You may change the PDB parameter (which sets the PDB file loaded into the applet)
	~~\|LIGAND= <~~scene ~~name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=CZA:(6AR,11AS,11BR~~)~~-10-ACETYL-9-HYDROXY-7,7-DIMETHYL-2~~,~~6,6A,7,11A,11B-HEXAHYDRO-11H-PYRROLO[1',2':2,3]ISOINDOLO[4,5,6-CD]INDOL-11-ONE'>CZA</scene>~~		or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
	~~\|ACTIVITY= <span class='plainlinks'>[http://en~~.~~wikipedia.org/wiki/Calcium~~-~~transporting_ATPase Calcium~~-~~transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8] </span~~>		or leave the SCENE parameter empty for the default display.
	\|~~GENE~~=		-->
	\|~~DOMAIN~~=		{{STRUCTURE_2eas\| PDB=2eas \| SCENE= }}
	~~\|RELATEDENTRY=[[1wpg\|1WPG]], [[2agv\|2AGV]], [[2ear\|2EAR]], [[2eat\|2EAT]], [[2eau\|2EAU]]~~
	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eas OCA], [http://www.ebi.ac.uk/pdbsum/2eas PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2eas RCSB]</span>
	}}

	'''Crystal structure of the SR CA2+-ATPASE with bound CPA'''		'''Crystal structure of the SR CA2+-ATPASE with bound CPA'''
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	[[Category: Takahashi, M.]]		[[Category: Takahashi, M.]]
	[[Category: Toyoshima, C.]]		[[Category: Toyoshima, C.]]
	[[Category: ~~ca2~~+]]		[[Category: Ca2+]]
	[[Category: ~~had~~ fold]]		[[Category: Had fold]]
	[[Category: ~~ion~~ pump]]		[[Category: Hydrolase]]
	[[Category: ~~membrane~~ protein]]		[[Category: Ion pump]]
	[[Category: p-type atpase]]		[[Category: Membrane protein]]
			[[Category: P-type atpase]]
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