2ebf - Revision history

OCA at 10:36, 17 February 2021

2021-02-17T10:36:14Z

← Older revision		Revision as of 10:36, 17 February 2021
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	==Crystal structures reveal a thiol-protease like catalytic triad in the C-terminal region of Pasteurella multocida toxin==		==Crystal structures reveal a thiol-protease like catalytic triad in the C-terminal region of Pasteurella multocida toxin==
	<StructureSection load='2ebf' size='340' side='right' caption='[[2ebf]]~~, [[Resolution\|resolution]] 1.90Å~~' scene=''>		<StructureSection load='2ebf' size='340' side='right'caption='[[2ebf]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>~~[[2ebf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_multocidum"_lehmann_and_neumann_1899 "bacterium multocidum" lehmann and neumann 1899].~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EBF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2EBF FirstGlance]. <br>		<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EBF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PO:PHOSPHONATE'>2PO</scene>, <scene name='pdbligand=TRE:TREHALOSE'>TRE</scene></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ebf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ebf OCA], [https://pdbe.org/2ebf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ebf RCSB], [https://www.ebi.ac.uk/pdbsum/2ebf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ebf ProSAT]</span></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ebh\|2ebh]], [[2ec5\|2ec5]]</td></tr>~~
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">toxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=747 "Bacterium multocidum" Lehmann and Neumann 1899])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ebf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ebf OCA], [~~http~~://pdbe.org/2ebf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ebf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ebf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ebf ProSAT]</span></td></tr>
	</table>		</table>
	~~== Function ==~~
	[[http://www.uniprot.org/uniprot/TOXA_PASMD TOXA_PASMD]] This is a dermonecrotic toxin. This osteolytic toxin, induces bone resorption. Potent mitogen. This toxin is associated with the severe progressive form of the atrophic rhinitis, a major respiratory disease in pigs.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ebf ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ebf ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Pasteurella multocida toxin (PMT), one of the virulence factors produced by the bacteria, exerts its toxicity by up-regulating various signaling cascades downstream of the heterotrimeric GTPases Gq and G12/13 in an unknown fashion. Here, we present the crystal structure of the C-terminal region (residues 575-1,285) of PMT, which carries an intracellularly active moiety. The overall structure of C-terminal region of PMT displays a Trojan horse-like shape, composed of three domains with a "feet"-,"body"-, and "head"-type arrangement, which were designated C1, C2, and C3 from the N to the C terminus, respectively. The C1 domain, showing marked similarity in steric structure to the N-terminal domain of Clostridium difficile toxin B, was found to lead the toxin molecule to the plasma membrane. The C3 domain possesses the Cys-His-Asp catalytic triad that is organized only when the Cys is released from a disulfide bond. The steric alignment of the triad corresponded well to that of papain or other enzymes carrying Cys-His-Asp. PMT toxicities on target cells were completely abrogated when one of the amino acids constituting the triad was mutated. Our results indicate that PMT is an enzyme toxin carrying the cysteine protease-like catalytic triad dependent on the redox state and functions on the cytoplasmic face of the plasma membrane of target cells.

	Crystal structures reveal a thiol protease-like catalytic triad in the C-terminal region of Pasteurella multocida toxin.,Kitadokoro K, Kamitani S, Miyazawa M, Hanajima-Ozawa M, Fukui A, Miyake M, Horiguchi Y Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):5139-44. Epub 2007 Mar 14. PMID:17360394<ref>PMID:17360394</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2ebf" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bacterium multocidum lehmann and neumann 1899~~]]		[[Category: Large Structures]]
	[[Category: Horiguchi, Y]]		[[Category: Horiguchi Y]]
	[[Category: Kamitani, S]]		[[Category: Kamitani S]]
	[[Category: Kitadokoro, K]]		[[Category: Kitadokoro K]]
	~~[[Category: Pasteurella multocida toxin]]~~
	~~[[Category: Toxin]]~~
	~~[[Category: Trojan horse-like fold~~]]

OCA at 06:32, 6 June 2018

2018-06-06T06:32:11Z

← Older revision		Revision as of 06:32, 6 June 2018
Line 1:		Line 1:

	==Crystal structures reveal a thiol-protease like catalytic triad in the C-terminal region of Pasteurella multocida toxin==		==Crystal structures reveal a thiol-protease like catalytic triad in the C-terminal region of Pasteurella multocida toxin==
	<StructureSection load='2ebf' size='340' side='right' caption='[[2ebf]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2ebf' size='340' side='right' caption='[[2ebf]], [[Resolution\|resolution]] 1.90Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ebh\|2ebh]], [[2ec5\|2ec5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ebh\|2ebh]], [[2ec5\|2ec5]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">toxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=747 "Bacterium multocidum" Lehmann and Neumann 1899])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">toxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=747 "Bacterium multocidum" Lehmann and Neumann 1899])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ebf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ebf OCA], [http://pdbe.org/2ebf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ebf RCSB], [http://www.ebi.ac.uk/pdbsum/2ebf PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ebf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ebf OCA], [http://pdbe.org/2ebf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ebf RCSB], [http://www.ebi.ac.uk/pdbsum/2ebf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ebf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eb/2ebf_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eb/2ebf_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 22:06, 7 February 2016

2016-02-07T22:06:33Z

← Older revision		Revision as of 22:06, 7 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ebf ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 05:21, 10 September 2015

2015-09-10T05:21:53Z

← Older revision		Revision as of 05:21, 10 September 2015
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	<StructureSection load='2ebf' size='340' side='right' caption='[[2ebf]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2ebf' size='340' side='right' caption='[[2ebf]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ebf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pasteurella_multocida Pasteurella multocida~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EBF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EBF FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ebf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_multocidum"_lehmann_and_neumann_1899 "bacterium multocidum" lehmann and neumann 1899]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EBF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EBF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PO:PHOSPHONATE'>2PO</scene>, <scene name='pdbligand=TRE:TREHALOSE'>TRE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PO:PHOSPHONATE'>2PO</scene>, <scene name='pdbligand=TRE:TREHALOSE'>TRE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ebh\|2ebh]], [[2ec5\|2ec5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ebh\|2ebh]], [[2ec5\|2ec5]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">toxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=747 ~~Pasteurella multocida~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">toxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=747 "Bacterium multocidum" Lehmann and Neumann 1899])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ebf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ebf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ebf RCSB], [http://www.ebi.ac.uk/pdbsum/2ebf PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ebf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ebf OCA], [http://pdbe.org/2ebf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ebf RCSB], [http://www.ebi.ac.uk/pdbsum/2ebf PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/~~TOXA_PASMU TOXA_PASMU~~]] This is a dermonecrotic toxin. This osteolytic toxin, induces bone resorption. Potent mitogen. This toxin is associated with the severe progressive form of the atrophic rhinitis, a major respiratory disease in pigs.		[[http://www.uniprot.org/uniprot/TOXA_PASMD TOXA_PASMD]] This is a dermonecrotic toxin. This osteolytic toxin, induces bone resorption. Potent mitogen. This toxin is associated with the severe progressive form of the atrophic rhinitis, a major respiratory disease in pigs.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2ebf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Pasteurella multocida~~]]		[[Category: Bacterium multocidum lehmann and neumann 1899]]
	[[Category: Horiguchi, Y]]		[[Category: Horiguchi, Y]]
	[[Category: Kamitani, S]]		[[Category: Kamitani, S]]

OCA at 18:32, 24 December 2014

2014-12-24T18:32:14Z

← Older revision		Revision as of 18:32, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ebf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EBF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EBF FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ebf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EBF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EBF FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PO:PHOSPHONATE'>2PO</scene>, <scene name='pdbligand=TRE:TREHALOSE'>TRE</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PO:PHOSPHONATE'>2PO</scene>, <scene name='pdbligand=TRE:TREHALOSE'>TRE</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ebh\|2ebh]], [[2ec5\|2ec5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ebh\|2ebh]], [[2ec5\|2ec5]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">toxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=747 Pasteurella multocida])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">toxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=747 Pasteurella multocida])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ebf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ebf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ebf RCSB], [http://www.ebi.ac.uk/pdbsum/2ebf PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ebf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ebf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ebf RCSB], [http://www.ebi.ac.uk/pdbsum/2ebf PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/TOXA_PASMU TOXA_PASMU]] This is a dermonecrotic toxin. This osteolytic toxin, induces bone resorption. Potent mitogen. This toxin is associated with the severe progressive form of the atrophic rhinitis, a major respiratory disease in pigs.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Pasteurella multocida]]		[[Category: Pasteurella multocida]]
	[[Category: Horiguchi, Y.]]		[[Category: Horiguchi, Y]]
	[[Category: Kamitani, S.]]		[[Category: Kamitani, S]]
	[[Category: Kitadokoro, K.]]		[[Category: Kitadokoro, K]]
	[[Category: Pasteurella multocida toxin]]		[[Category: Pasteurella multocida toxin]]
	[[Category: Toxin]]		[[Category: Toxin]]
	[[Category: Trojan horse-like fold]]		[[Category: Trojan horse-like fold]]

OCA at 01:33, 30 September 2014

2014-09-30T01:33:58Z

← Older revision		Revision as of 01:33, 30 September 2014
Line 1:		Line 1:
	[[Image:~~2ebf~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structures reveal a thiol-protease like catalytic triad in the C-terminal region of Pasteurella multocida toxin==
			<StructureSection load='2ebf' size='340' side='right' caption='[[2ebf]], [[Resolution\|resolution]] 1.90Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2ebf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EBF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EBF FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PO:PHOSPHONATE'>2PO</scene>, <scene name='pdbligand=TRE:TREHALOSE'>TRE</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ebh\|2ebh]], [[2ec5\|2ec5]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">toxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=747 Pasteurella multocida])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ebf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ebf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ebf RCSB], [http://www.ebi.ac.uk/pdbsum/2ebf PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eb/2ebf_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Pasteurella multocida toxin (PMT), one of the virulence factors produced by the bacteria, exerts its toxicity by up-regulating various signaling cascades downstream of the heterotrimeric GTPases Gq and G12/13 in an unknown fashion. Here, we present the crystal structure of the C-terminal region (residues 575-1,285) of PMT, which carries an intracellularly active moiety. The overall structure of C-terminal region of PMT displays a Trojan horse-like shape, composed of three domains with a "feet"-,"body"-, and "head"-type arrangement, which were designated C1, C2, and C3 from the N to the C terminus, respectively. The C1 domain, showing marked similarity in steric structure to the N-terminal domain of Clostridium difficile toxin B, was found to lead the toxin molecule to the plasma membrane. The C3 domain possesses the Cys-His-Asp catalytic triad that is organized only when the Cys is released from a disulfide bond. The steric alignment of the triad corresponded well to that of papain or other enzymes carrying Cys-His-Asp. PMT toxicities on target cells were completely abrogated when one of the amino acids constituting the triad was mutated. Our results indicate that PMT is an enzyme toxin carrying the cysteine protease-like catalytic triad dependent on the redox state and functions on the cytoplasmic face of the plasma membrane of target cells.

	~~{{STRUCTURE_2ebf\| PDB=2ebf \| SCENE= }}~~		Crystal structures reveal a thiol protease-like catalytic triad in the C-terminal region of Pasteurella multocida toxin.,Kitadokoro K, Kamitani S, Miyazawa M, Hanajima-Ozawa M, Fukui A, Miyake M, Horiguchi Y Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):5139-44. Epub 2007 Mar 14. PMID:17360394<ref>PMID:17360394</ref>

	~~===Crystal structures reveal~~ a ~~thiol-protease like catalytic triad in~~ the ~~C-terminal region of Pasteurella multocida toxin===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_17360394}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2ebf]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pasteurella_multocida Pasteurella multocida]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2EBF OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:017360394<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Pasteurella multocida]]		[[Category: Pasteurella multocida]]
	[[Category: Horiguchi, Y.]]		[[Category: Horiguchi, Y.]]

OCA: Protected "2ebf" [edit=sysop:move=sysop]

2013-01-06T10:48:20Z

Protected "2ebf" [edit=sysop:move=sysop]

← Older revision	Revision as of 10:48, 6 January 2013
(No difference)

OCA at 10:48, 6 January 2013

2013-01-06T10:48:15Z

← Older revision		Revision as of 10:48, 6 January 2013
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	~~{{Seed}}~~
	[[Image:2ebf.png\|left\|200px]]		[[Image:2ebf.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2ebf", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2ebf\| PDB=2ebf \| SCENE= }}		{{STRUCTURE_2ebf\| PDB=2ebf \| SCENE= }}

	===Crystal structures reveal a thiol-protease like catalytic triad in the C-terminal region of Pasteurella multocida toxin===		===Crystal structures reveal a thiol-protease like catalytic triad in the C-terminal region of Pasteurella multocida toxin===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_17360394}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 17360394 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_17360394}}		{{ABSTRACT_PUBMED_17360394}}

	==About this Structure==		==About this Structure==
	~~2EBF~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EBF OCA].		[[2ebf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EBF OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~17360394~~</ref><references group="xtra"/>		<ref group="xtra">PMID:017360394</ref><references group="xtra"/>
	[[Category: Pasteurella multocida]]		[[Category: Pasteurella multocida]]
	[[Category: Horiguchi, Y.]]		[[Category: Horiguchi, Y.]]
Line 29:		Line 17:
	[[Category: Kitadokoro, K.]]		[[Category: Kitadokoro, K.]]
	[[Category: Pasteurella multocida toxin]]		[[Category: Pasteurella multocida toxin]]
			[[Category: Toxin]]
	[[Category: Trojan horse-like fold]]		[[Category: Trojan horse-like fold]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 16:48:49 2009''~~

OCA at 14:48, 16 February 2009

2009-02-16T14:48:51Z

← Older revision		Revision as of 14:48, 16 February 2009
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	==About this Structure==		==About this Structure==
	2EBF is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EBF OCA].		2EBF is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EBF OCA].

	==Reference==		==Reference==
	Crystal structures reveal a thiol protease-like catalytic triad in the C-terminal region of Pasteurella multocida toxin., Kitadokoro K, Kamitani S, Miyazawa M, Hanajima-Ozawa M, Fukui A, Miyake M, Horiguchi Y, Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):5139-44. Epub 2007 Mar 14. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/17360394 17360394]~~		<ref group="xtra">PMID:17360394</ref><references group="xtra"/>
	[[Category: Pasteurella multocida]]		[[Category: Pasteurella multocida]]
	~~[[Category: Single protein]]~~
	[[Category: Horiguchi, Y.]]		[[Category: Horiguchi, Y.]]
	[[Category: Kamitani, S.]]		[[Category: Kamitani, S.]]
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	[[Category: Trojan horse-like fold]]		[[Category: Trojan horse-like fold]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun Jul 27 20~~:04:~~09 2008~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 16:48:49 2009''

OCA at 17:04, 27 July 2008

2008-07-27T17:04:13Z

← Older revision		Revision as of 17:04, 27 July 2008
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	[[Image:2ebf.~~jpg~~\|left\|200px]]		{{Seed}}
			[[Image:2ebf.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_2ebf\| PDB=2ebf \| SCENE= }}		{{STRUCTURE_2ebf\| PDB=2ebf \| SCENE= }}

	~~'''~~Crystal structures reveal a thiol-protease like catalytic triad in the C-terminal region of Pasteurella multocida toxin~~'''~~		===Crystal structures reveal a thiol-protease like catalytic triad in the C-terminal region of Pasteurella multocida toxin===


	~~==Overview==~~		<!--
	Pasteurella multocida toxin (PMT), one of the virulence factors produced by the bacteria, exerts its toxicity by up-regulating various signaling cascades downstream of the heterotrimeric GTPases Gq and G12/13 in an unknown fashion. Here, we present the crystal structure of the C-~~terminal region (residues 575~~-~~1,285) of PMT, which carries an intracellularly active moiety.~~ The ~~overall structure of C-terminal region of PMT displays a Trojan horse-like shape, composed of three domains with a "feet"-,"body"-, and "head"-type arrangement~~, ~~which were designated C1~~, ~~C2, and C3 from~~ the N to the ~~C terminus, respectively~~. ~~The C1 domain~~, showing marked similarity in steric structure to the N-terminal domain of Clostridium difficile toxin B, was found to lead the toxin molecule to the plasma membrane. The C3 domain possesses the Cys-His-Asp catalytic triad that is ~~organized only when~~ the ~~Cys is released from a disulfide bond~~. ~~The steric alignment of the triad corresponded well to that of papain or other enzymes carrying Cys~~-~~His~~-Asp. PMT toxicities on target cells were completely abrogated when one of the amino acids constituting the triad was mutated. Our results indicate that PMT is an enzyme toxin carrying the cysteine protease-like catalytic triad dependent on the redox state and functions on the cytoplasmic face of the plasma membrane of target cells.		The line below this paragraph, {{ABSTRACT_PUBMED_17360394}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 17360394 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_17360394}}

	==About this Structure==		==About this Structure==
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	[[Category: Pasteurella multocida toxin]]		[[Category: Pasteurella multocida toxin]]
	[[Category: Trojan horse-like fold]]		[[Category: Trojan horse-like fold]]
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