2eex - Revision history

OCA at 08:39, 25 October 2023

2023-10-25T08:39:01Z

← Older revision		Revision as of 08:39, 25 October 2023
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	<StructureSection load='2eex' size='340' side='right'caption='[[2eex]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2eex' size='340' side='right'caption='[[2eex]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2eex]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"ruminiclostridium_thermocellum"_(viljoen_et_al._1926)_yutin_and_galperin_2013 "ruminiclostridium thermocellum" (viljoen et al. 1926) yutin and galperin 2013~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EEX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EEX FirstGlance]. <br>		<table><tr><td colspan='2'>[[2eex]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetivibrio_thermocellus Acetivibrio thermocellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EEX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EEX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:~~ZINC+ION~~'>ZN</scene><~~/td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;~~'>~~[[2e4t\|2e4t]], [[2e0p\|2e0p]]~~</~~div></td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PRD_900016:beta-cellopentaose'>PRD_900016</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td~~>~~<td class="sblockDat">Cel44A ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1515 "Ruminiclostridium thermocellum" (Viljoen et al. 1926) Yutin and Galperin 2013])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eex OCA], [https://pdbe.org/2eex PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eex RCSB], [https://www.ebi.ac.uk/pdbsum/2eex PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eex ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eex OCA], [https://pdbe.org/2eex PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eex RCSB], [https://www.ebi.ac.uk/pdbsum/2eex PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eex ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/A3DD30_ACET2 A3DD30_ACET2]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Acetivibrio thermocellus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Karita, S]]		[[Category: Karita S]]
	[[Category: Kitago, Y]]		[[Category: Kitago Y]]
	[[Category: Sakka, K]]		[[Category: Sakka K]]
	[[Category: Tanaka, I]]		[[Category: Tanaka I]]
	[[Category: Watanabe, N]]		[[Category: Watanabe N]]
	~~[[Category: Gh family 44]]~~
	~~[[Category: Glycoside hydrolase]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: The composite domain of gh family 5]]~~
	~~[[Category: Tim barrel~~]]

OCA at 06:55, 10 November 2021

2021-11-10T06:55:22Z

← Older revision		Revision as of 06:55, 10 November 2021
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	==Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum==		==Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum==
	<StructureSection load='2eex' size='340' side='right'caption='[[2eex]]' scene=''>		<StructureSection load='2eex' size='340' side='right'caption='[[2eex]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EEX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EEX FirstGlance]. <br>		<table><tr><td colspan='2'>[[2eex]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"ruminiclostridium_thermocellum"_(viljoen_et_al._1926)_yutin_and_galperin_2013 "ruminiclostridium thermocellum" (viljoen et al. 1926) yutin and galperin 2013]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EEX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EEX FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eex OCA], [https://pdbe.org/2eex PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eex RCSB], [https://www.ebi.ac.uk/pdbsum/2eex PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eex ProSAT]</span></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2e4t\|2e4t]], [[2e0p\|2e0p]]</div></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cel44A ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1515 "Ruminiclostridium thermocellum" (Viljoen et al. 1926) Yutin and Galperin 2013])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eex OCA], [https://pdbe.org/2eex PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eex RCSB], [https://www.ebi.ac.uk/pdbsum/2eex PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eex ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eex ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eex ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The crystal structure of Cel44A, which is one of the enzymatic components of the cellulosome of Clostridium thermocellum, was solved at a resolution of 0.96 A. This enzyme belongs to glycoside hydrolase family (GH family) 44. The structure reveals that Cel44A consists of a TIM-like barrel domain and a beta-sandwich domain. The wild-type and the E186Q mutant structures complexed with substrates suggest that two glutamic acid residues, Glu(186) and Glu(359), are the active residues of the enzyme. Biochemical experiments were performed to confirm this idea. The structural features indicate that GH family 44 belongs to clan GH-A and that the reaction catalyzed by Cel44A is retaining type hydrolysis. The stereochemical course of hydrolysis was confirmed by a (1)H NMR experiment using the reduced cellooligosaccharide as a substrate.

			Crystal structure of Cel44A, a glycoside hydrolase family 44 endoglucanase from Clostridium thermocellum.,Kitago Y, Karita S, Watanabe N, Kamiya M, Aizawa T, Sakka K, Tanaka I J Biol Chem. 2007 Dec 7;282(49):35703-11. Epub 2007 Sep 28. PMID:17905739<ref>PMID:17905739</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 2eex" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Glucanase 3D structures\|Glucanase 3D structures]]		*[[Glucanase 3D structures\|Glucanase 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Karita S]]		[[Category: Karita, S]]
	[[Category: Kitago Y]]		[[Category: Kitago, Y]]
	[[Category: Sakka K]]		[[Category: Sakka, K]]
	[[Category: Tanaka I]]		[[Category: Tanaka, I]]
	[[Category: Watanabe N]]		[[Category: Watanabe, N]]
			[[Category: Gh family 44]]
			[[Category: Glycoside hydrolase]]
			[[Category: Hydrolase]]
			[[Category: The composite domain of gh family 5]]
			[[Category: Tim barrel]]

OCA at 10:36, 17 February 2021

2021-02-17T10:36:19Z

← Older revision		Revision as of 10:36, 17 February 2021
Line 1:		Line 1:

	==Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum==		==Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum==
	<StructureSection load='2eex' size='340' side='right' caption='[[2eex]]~~, [[Resolution\|resolution]] 2.00Å~~' scene=''>		<StructureSection load='2eex' size='340' side='right'caption='[[2eex]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>~~[[2eex]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"ruminiclostridium_thermocellum"_yutin_and_galperin_2013 "ruminiclostridium thermocellum" yutin and galperin 2013].~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EEX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2EEX FirstGlance]. <br>		<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EEX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EEX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CE5:CELLOPENTAOSE'>CE5</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eex OCA], [https://pdbe.org/2eex PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eex RCSB], [https://www.ebi.ac.uk/pdbsum/2eex PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eex ProSAT]</span></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2e4t\|2e4t]], [[2e0p\|2e0p]]</td></tr>~~
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cel44A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1515 "Ruminiclostridium thermocellum" Yutin and Galperin 2013])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2eex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eex OCA], [~~http~~://pdbe.org/2eex PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2eex RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2eex PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2eex ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 19:		Line 16:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eex ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eex ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The crystal structure of Cel44A, which is one of the enzymatic components of the cellulosome of Clostridium thermocellum, was solved at a resolution of 0.96 A. This enzyme belongs to glycoside hydrolase family (GH family) 44. The structure reveals that Cel44A consists of a TIM-like barrel domain and a beta-sandwich domain. The wild-type and the E186Q mutant structures complexed with substrates suggest that two glutamic acid residues, Glu(186) and Glu(359), are the active residues of the enzyme. Biochemical experiments were performed to confirm this idea. The structural features indicate that GH family 44 belongs to clan GH-A and that the reaction catalyzed by Cel44A is retaining type hydrolysis. The stereochemical course of hydrolysis was confirmed by a (1)H NMR experiment using the reduced cellooligosaccharide as a substrate.

	Crystal structure of Cel44A, a glycoside hydrolase family 44 endoglucanase from Clostridium thermocellum.,Kitago Y, Karita S, Watanabe N, Kamiya M, Aizawa T, Sakka K, Tanaka I J Biol Chem. 2007 Dec 7;282(49):35703-11. Epub 2007 Sep 28. PMID:17905739<ref>PMID:17905739</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2eex" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Glucanase\|Glucanase]]		*[[Glucanase 3D structures\|Glucanase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Ruminiclostridium thermocellum yutin and galperin 2013~~]]		[[Category: Large Structures]]
	[[Category: Karita, S]]		[[Category: Karita S]]
	[[Category: Kitago, Y]]		[[Category: Kitago Y]]
	[[Category: Sakka, K]]		[[Category: Sakka K]]
	[[Category: Tanaka, I]]		[[Category: Tanaka I]]
	[[Category: Watanabe, N]]		[[Category: Watanabe N]]
	~~[[Category: Gh family 44]]~~
	~~[[Category: Glycoside hydrolase]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: The composite domain of gh family 5]]~~
	~~[[Category: Tim barrel~~]]

OCA at 06:44, 6 June 2018

2018-06-06T06:44:01Z

← Older revision		Revision as of 06:44, 6 June 2018
Line 1:		Line 1:

	==Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum==		==Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum==
	<StructureSection load='2eex' size='340' side='right' caption='[[2eex]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2eex' size='340' side='right' caption='[[2eex]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2eex]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Atcc_27405 Atcc 27405~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EEX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EEX FirstGlance]. <br>		<table><tr><td colspan='2'>[[2eex]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"ruminiclostridium_thermocellum"_yutin_and_galperin_2013 "ruminiclostridium thermocellum" yutin and galperin 2013]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EEX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EEX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CE5:CELLOPENTAOSE'>CE5</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CE5:CELLOPENTAOSE'>CE5</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2e4t\|2e4t]], [[2e0p\|2e0p]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2e4t\|2e4t]], [[2e0p\|2e0p]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cel44A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1515 ~~ATCC 27405~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cel44A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1515 "Ruminiclostridium thermocellum" Yutin and Galperin 2013])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eex OCA], [http://pdbe.org/2eex PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2eex RCSB], [http://www.ebi.ac.uk/pdbsum/2eex PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eex OCA], [http://pdbe.org/2eex PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2eex RCSB], [http://www.ebi.ac.uk/pdbsum/2eex PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2eex ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ee/2eex_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ee/2eex_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Atcc 27405~~]]		[[Category: Ruminiclostridium thermocellum yutin and galperin 2013]]
	[[Category: Karita, S]]		[[Category: Karita, S]]
	[[Category: Kitago, Y]]		[[Category: Kitago, Y]]

OCA at 18:38, 9 February 2016

2016-02-09T18:38:09Z

← Older revision		Revision as of 18:38, 9 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eex ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 00:57, 11 September 2015

2015-09-11T00:57:59Z

← Older revision		Revision as of 00:57, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2eex' size='340' side='right' caption='[[2eex]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2eex' size='340' side='right' caption='[[2eex]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2eex]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Clostridium_thermocellum Clostridium thermocellum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EEX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EEX FirstGlance]. <br>		<table><tr><td colspan='2'>[[2eex]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_27405 Atcc 27405]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EEX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EEX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CE5:CELLOPENTAOSE'>CE5</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CE5:CELLOPENTAOSE'>CE5</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2e4t\|2e4t]], [[2e0p\|2e0p]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2e4t\|2e4t]], [[2e0p\|2e0p]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cel44A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1515 ~~Clostridium thermocellum~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cel44A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1515 ATCC 27405])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eex OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2eex RCSB], [http://www.ebi.ac.uk/pdbsum/2eex PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eex OCA], [http://pdbe.org/2eex PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2eex RCSB], [http://www.ebi.ac.uk/pdbsum/2eex PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2eex" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Clostridium thermocellum~~]]		[[Category: Atcc 27405]]
	[[Category: Karita, S]]		[[Category: Karita, S]]
	[[Category: Kitago, Y]]		[[Category: Kitago, Y]]

OCA at 17:46, 15 January 2015

2015-01-15T17:46:08Z

← Older revision		Revision as of 17:46, 15 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2eex]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EEX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EEX FirstGlance]. <br>		<table><tr><td colspan='2'>[[2eex]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EEX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EEX FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CE5:CELLOPENTAOSE'>CE5</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CE5:CELLOPENTAOSE'>CE5</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2e4t\|2e4t]], [[2e0p\|2e0p]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2e4t\|2e4t]], [[2e0p\|2e0p]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cel44A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1515 Clostridium thermocellum])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cel44A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1515 Clostridium thermocellum])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eex OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2eex RCSB], [http://www.ebi.ac.uk/pdbsum/2eex PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eex OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2eex RCSB], [http://www.ebi.ac.uk/pdbsum/2eex PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Clostridium thermocellum]]		[[Category: Clostridium thermocellum]]
	[[Category: Karita, S.]]		[[Category: Karita, S]]
	[[Category: Kitago, Y.]]		[[Category: Kitago, Y]]
	[[Category: Sakka, K.]]		[[Category: Sakka, K]]
	[[Category: Tanaka, I.]]		[[Category: Tanaka, I]]
	[[Category: Watanabe, N~~.]]~~		[[Category: Watanabe, N]]
	~~[[Category: 30]]~~
	~~[[Category: 39]]~~
	~~[[Category: 51~~]]
	[[Category: Gh family 44]]		[[Category: Gh family 44]]
	[[Category: Glycoside hydrolase]]		[[Category: Glycoside hydrolase]]

OCA at 03:59, 29 September 2014

2014-09-29T03:59:12Z

← Older revision		Revision as of 03:59, 29 September 2014
Line 1:		Line 1:
	[[Image:~~2eex~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum==
			<StructureSection load='2eex' size='340' side='right' caption='[[2eex]], [[Resolution\|resolution]] 2.00Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2eex]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EEX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EEX FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CE5:CELLOPENTAOSE'>CE5</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2e4t\|2e4t]], [[2e0p\|2e0p]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cel44A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1515 Clostridium thermocellum])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eex OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2eex RCSB], [http://www.ebi.ac.uk/pdbsum/2eex PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ee/2eex_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The crystal structure of Cel44A, which is one of the enzymatic components of the cellulosome of Clostridium thermocellum, was solved at a resolution of 0.96 A. This enzyme belongs to glycoside hydrolase family (GH family) 44. The structure reveals that Cel44A consists of a TIM-like barrel domain and a beta-sandwich domain. The wild-type and the E186Q mutant structures complexed with substrates suggest that two glutamic acid residues, Glu(186) and Glu(359), are the active residues of the enzyme. Biochemical experiments were performed to confirm this idea. The structural features indicate that GH family 44 belongs to clan GH-A and that the reaction catalyzed by Cel44A is retaining type hydrolysis. The stereochemical course of hydrolysis was confirmed by a (1)H NMR experiment using the reduced cellooligosaccharide as a substrate.

	~~{{STRUCTURE_2eex\| PDB=2eex \| SCENE= }}~~		Crystal structure of Cel44A, a glycoside hydrolase family 44 endoglucanase from Clostridium thermocellum.,Kitago Y, Karita S, Watanabe N, Kamiya M, Aizawa T, Sakka K, Tanaka I J Biol Chem. 2007 Dec 7;282(49):35703-11. Epub 2007 Sep 28. PMID:17905739<ref>PMID:17905739</ref>

	~~===Crystal structure of Cel44A~~, ~~GH family 44 endoglucanase from Clostridium thermocellum===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_17905739}}~~

	~~==About this Structure==~~
	~~[[2eex]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Clostridium_thermocellum Clostridium thermocellum]~~. ~~Full crystallographic information is available from [http://oca.weizmann.ac.il~~/~~oca-bin/ocashort?id=2EEX OCA].~~

	==See Also==		==See Also==
	*[[Glucanase\|Glucanase]]		*[[Glucanase\|Glucanase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:017905739<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Clostridium thermocellum]]		[[Category: Clostridium thermocellum]]
	[[Category: Karita, S.]]		[[Category: Karita, S.]]

OCA at 14:45, 20 October 2012

2012-10-20T14:45:41Z

← Older revision		Revision as of 14:45, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[2eex]] is a 1 chain structure ~~of [[Glucanase]]~~ with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EEX OCA].		[[2eex]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EEX OCA].

	==See Also==		==See Also==

OCA: Protected "2eex" [edit=sysop:move=sysop]

2012-07-26T16:09:54Z

Protected "2eex" [edit=sysop:move=sysop]

← Older revision	Revision as of 16:09, 26 July 2012
(No difference)