2eip - Revision history

OCA at 09:19, 14 February 2024

2024-02-14T09:19:18Z

← Older revision		Revision as of 09:19, 14 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2eip]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1eip 1eip]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EIP FirstGlance]. <br>		<table><tr><td colspan='2'>[[2eip]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1eip 1eip]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EIP FirstGlance]. <br>
	</td></tr><tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"~~><span class~~=~~'plainlinks'~~>[~~https://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase~~]~~, with EC number [https://www~~.~~brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] </span>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eip OCA], [https://pdbe.org/2eip PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eip RCSB], [https://www.ebi.ac.uk/pdbsum/2eip PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eip ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eip OCA], [https://pdbe.org/2eip PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eip RCSB], [https://www.ebi.ac.uk/pdbsum/2eip PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eip ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/IPYR_ECOLI IPYR_ECOLI]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eip ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eip ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The refined crystal structures of hexameric soluble inorganic pyrophosphatase from E. coli (E-PPase) are reported to R factors of 18.7 and 18.3% at 2.15 and 2.2 A, respectively. The first contains one independent monomer; the other, two independent monomers, in an R32 unit cell. Because the E-PPase monomer is small with a large open active site, there are relatively few hydrophobic interactions that connect the active-site loops to the five-stranded twisted beta-barrel that is the hydrophobic core of the molecule. The active-site loops are, however, held in place by interactions between monomers around the threefold and twofold symmetry axes of the D(3) hexamer. Consequently, mutations of active-site residues (such as Glu20 and Lysl04) often affect protein stability and oligomeric structure. Conversely, mutations of residues in the interface between monomers (such as His136 and Hisl40) not only affect oligomeric structure but also affect active-site function. The effects of the H136Q and H140Q variants can be explained by the extended ionic interaction between H140, D143 and H136' of the neighbouring monomer. This interaction is further buttressed by an extensive hydrogen-bonding network that appears to explain why the E-PPase hexamer is so stable and also why the H136Q and H140Q variant proteins are less stable as hexamers.

	Structure of Escherichia coli inorganic pyrophosphatase at 2.2 A resolution.,Kankare J, Salminen T, Lahti R, Cooperman BS, Baykov AA, Goldman A Acta Crystallogr D Biol Crystallogr. 1996 May 1;52(Pt 3):551-63. PMID:15299678<ref>PMID:15299678</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2eip" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Inorganic pyrophosphatase 3D structures\|Inorganic pyrophosphatase 3D structures]]		*[[Inorganic pyrophosphatase 3D structures\|Inorganic pyrophosphatase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	~~[[Category: Inorganic diphosphatase]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Goldman, A]]		[[Category: Goldman A]]
	[[Category: Kankare~~, J A~~]]		[[Category: Kankare JA]]
	[[Category: Salminen, T]]		[[Category: Salminen T]]
	~~[[Category: Hydrolase]]~~
	~~[[Category: Inorganic pyrophoshatase]]~~
	~~[[Category: Inorganic pyrophosphatase]]~~
	~~[[Category: Magnesium~~]]

OCA at 17:24, 15 December 2021

2021-12-15T17:24:51Z

← Older revision		Revision as of 17:24, 15 December 2021
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	<StructureSection load='2eip' size='340' side='right'caption='[[2eip]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2eip' size='340' side='right'caption='[[2eip]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2eip]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1eip 1eip]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2EIP FirstGlance]. <br>		<table><tr><td colspan='2'>[[2eip]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1eip 1eip]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EIP FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2eip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eip OCA], [~~http~~://pdbe.org/2eip PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2eip RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2eip PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2eip ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eip OCA], [https://pdbe.org/2eip PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eip RCSB], [https://www.ebi.ac.uk/pdbsum/2eip PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eip ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 08:33, 11 March 2020

2020-03-11T08:33:41Z

← Older revision		Revision as of 08:33, 11 March 2020
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	==INORGANIC PYROPHOSPHATASE==		==INORGANIC PYROPHOSPHATASE==
	<StructureSection load='2eip' size='340' side='right' caption='[[2eip]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2eip' size='340' side='right'caption='[[2eip]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2eip]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1eip 1eip]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EIP FirstGlance]. <br>		<table><tr><td colspan='2'>[[2eip]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1eip 1eip]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EIP FirstGlance]. <br>
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	==See Also==		==See Also==
	*[[Inorganic pyrophosphatase\|Inorganic pyrophosphatase]]		*[[Inorganic pyrophosphatase 3D structures\|Inorganic pyrophosphatase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Inorganic diphosphatase]]		[[Category: Inorganic diphosphatase]]
			[[Category: Large Structures]]
	[[Category: Goldman, A]]		[[Category: Goldman, A]]
	[[Category: Kankare, J A]]		[[Category: Kankare, J A]]

OCA at 06:55, 6 June 2018

2018-06-06T06:55:43Z

← Older revision		Revision as of 06:55, 6 June 2018
Line 1:		Line 1:

	==INORGANIC PYROPHOSPHATASE==		==INORGANIC PYROPHOSPHATASE==
	<StructureSection load='2eip' size='340' side='right' caption='[[2eip]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2eip' size='340' side='right' caption='[[2eip]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2eip]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1eip 1eip]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EIP FirstGlance]. <br>		<table><tr><td colspan='2'>[[2eip]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1eip 1eip]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EIP FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eip OCA], [http://pdbe.org/2eip PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2eip RCSB], [http://www.ebi.ac.uk/pdbsum/2eip PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eip OCA], [http://pdbe.org/2eip PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2eip RCSB], [http://www.ebi.ac.uk/pdbsum/2eip PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2eip ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 10:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ei/2eip_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ei/2eip_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eip ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 00:47, 12 September 2015

2015-09-12T00:47:26Z

← Older revision		Revision as of 00:47, 12 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[2eip]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1eip 1eip]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EIP FirstGlance]. <br>		<table><tr><td colspan='2'>[[2eip]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1eip 1eip]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EIP FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eip OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2eip RCSB], [http://www.ebi.ac.uk/pdbsum/2eip PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eip OCA], [http://pdbe.org/2eip PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2eip RCSB], [http://www.ebi.ac.uk/pdbsum/2eip PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2eip" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 18:08, 15 January 2015

2015-01-15T18:08:14Z

← Older revision		Revision as of 18:08, 15 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2eip]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1eip 1eip]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EIP FirstGlance]. <br>		<table><tr><td colspan='2'>[[2eip]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1eip 1eip]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EIP FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eip OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2eip RCSB], [http://www.ebi.ac.uk/pdbsum/2eip PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eip OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2eip RCSB], [http://www.ebi.ac.uk/pdbsum/2eip PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Inorganic diphosphatase]]		[[Category: Inorganic diphosphatase]]
	[[Category: Goldman, A.]]		[[Category: Goldman, A]]
	[[Category: Kankare, J A.]]		[[Category: Kankare, J A]]
	[[Category: Salminen, T.]]		[[Category: Salminen, T]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Inorganic pyrophoshatase]]		[[Category: Inorganic pyrophoshatase]]
	[[Category: Inorganic pyrophosphatase]]		[[Category: Inorganic pyrophosphatase]]
	[[Category: Magnesium]]		[[Category: Magnesium]]

OCA at 05:13, 29 September 2014

2014-09-29T05:13:09Z

← Older revision		Revision as of 05:13, 29 September 2014
Line 1:		Line 1:
	[[Image:~~2eip~~.~~png~~\|~~left~~\|~~200px~~]]		==INORGANIC PYROPHOSPHATASE==
			<StructureSection load='2eip' size='340' side='right' caption='[[2eip]], [[Resolution\|resolution]] 2.20Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2eip]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1eip 1eip]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EIP FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eip OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2eip RCSB], [http://www.ebi.ac.uk/pdbsum/2eip PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ei/2eip_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The refined crystal structures of hexameric soluble inorganic pyrophosphatase from E. coli (E-PPase) are reported to R factors of 18.7 and 18.3% at 2.15 and 2.2 A, respectively. The first contains one independent monomer; the other, two independent monomers, in an R32 unit cell. Because the E-PPase monomer is small with a large open active site, there are relatively few hydrophobic interactions that connect the active-site loops to the five-stranded twisted beta-barrel that is the hydrophobic core of the molecule. The active-site loops are, however, held in place by interactions between monomers around the threefold and twofold symmetry axes of the D(3) hexamer. Consequently, mutations of active-site residues (such as Glu20 and Lysl04) often affect protein stability and oligomeric structure. Conversely, mutations of residues in the interface between monomers (such as His136 and Hisl40) not only affect oligomeric structure but also affect active-site function. The effects of the H136Q and H140Q variants can be explained by the extended ionic interaction between H140, D143 and H136' of the neighbouring monomer. This interaction is further buttressed by an extensive hydrogen-bonding network that appears to explain why the E-PPase hexamer is so stable and also why the H136Q and H140Q variant proteins are less stable as hexamers.

	~~{{STRUCTURE_2eip\| PDB=2eip \| SCENE= }}~~		Structure of Escherichia coli inorganic pyrophosphatase at 2.2 A resolution.,Kankare J, Salminen T, Lahti R, Cooperman BS, Baykov AA, Goldman A Acta Crystallogr D Biol Crystallogr. 1996 May 1;52(Pt 3):551-63. PMID:15299678<ref>PMID:15299678</ref>

	~~===INORGANIC PYROPHOSPHATASE===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_15299678}}~~

	~~==About this Structure==~~
	~~[[2eip]] is~~ a ~~2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes~~ the ~~now removed PDB entry [http://oca~~.~~weizmann~~.ac.~~il/oca-bin/send-pdb?obs=1&id=1eip 1eip]. Full crystallographic information is available from [http:/~~/~~oca.weizmann.ac.il/oca-bin/ocashort?id=2EIP OCA].~~

	==See Also==		==See Also==
	*[[Inorganic pyrophosphatase\|Inorganic pyrophosphatase]]		*[[Inorganic pyrophosphatase\|Inorganic pyrophosphatase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:015299678<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Inorganic diphosphatase]]		[[Category: Inorganic diphosphatase]]

OCA at 21:56, 20 October 2012

2012-10-20T21:56:50Z

← Older revision		Revision as of 21:56, 20 October 2012
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	==About this Structure==		==About this Structure==
	[[2eip]] is a 2 chain structure ~~of [[Inorganic pyrophosphatase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1eip 1eip]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIP OCA].		[[2eip]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1eip 1eip]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIP OCA].

	==See Also==		==See Also==

OCA: Protected "2eip" [edit=sysop:move=sysop]

2012-07-27T14:44:18Z

Protected "2eip" [edit=sysop:move=sysop]

← Older revision	Revision as of 14:44, 27 July 2012
(No difference)

OCA at 14:44, 27 July 2012

2012-07-27T14:44:16Z

← Older revision		Revision as of 14:44, 27 July 2012
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	~~{{Seed}}~~
	[[Image:2eip.png\|left\|200px]]		[[Image:2eip.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2eip", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2eip\| PDB=2eip \| SCENE= }}		{{STRUCTURE_2eip\| PDB=2eip \| SCENE= }}

	===INORGANIC PYROPHOSPHATASE===		===INORGANIC PYROPHOSPHATASE===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_15299678}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 15299678 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_15299678}}		{{ABSTRACT_PUBMED_15299678}}

	==About this Structure==		==About this Structure==
	~~2EIP~~ is a 2 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1eip 1eip]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIP OCA].		[[2eip]] is a 2 chain structure of [[Inorganic pyrophosphatase]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1eip 1eip]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EIP OCA].

			==See Also==
			*[[Inorganic pyrophosphatase\|Inorganic pyrophosphatase]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~15299678~~</ref><references group="xtra"/>		<ref group="xtra">PMID:015299678</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Inorganic diphosphatase]]		[[Category: Inorganic diphosphatase]]
Line 31:		Line 22:
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Inorganic pyrophoshatase]]		[[Category: Inorganic pyrophoshatase]]
			[[Category: Inorganic pyrophosphatase]]
	[[Category: Magnesium]]		[[Category: Magnesium]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 08:33:00 2009''~~