2eo7 - Revision history

OCA at 08:43, 25 October 2023

2023-10-25T08:43:10Z

← Older revision		Revision as of 08:43, 25 October 2023
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	==Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum==		==Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum==
	<StructureSection load='2eo7' size='340' side='right'caption='[[2eo7]]' scene=''>		<StructureSection load='2eo7' size='340' side='right'caption='[[2eo7]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EO7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EO7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2eo7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetivibrio_thermocellus Acetivibrio thermocellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EO7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EO7 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eo7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eo7 OCA], [https://pdbe.org/2eo7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eo7 RCSB], [https://www.ebi.ac.uk/pdbsum/2eo7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eo7 ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PRD_900011:beta-cellotetraose'>PRD_900011</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eo7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eo7 OCA], [https://pdbe.org/2eo7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eo7 RCSB], [https://www.ebi.ac.uk/pdbsum/2eo7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eo7 ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/P71140_ACETH P71140_ACETH]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 16:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eo7 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eo7 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The crystal structure of Cel44A, which is one of the enzymatic components of the cellulosome of Clostridium thermocellum, was solved at a resolution of 0.96 A. This enzyme belongs to glycoside hydrolase family (GH family) 44. The structure reveals that Cel44A consists of a TIM-like barrel domain and a beta-sandwich domain. The wild-type and the E186Q mutant structures complexed with substrates suggest that two glutamic acid residues, Glu(186) and Glu(359), are the active residues of the enzyme. Biochemical experiments were performed to confirm this idea. The structural features indicate that GH family 44 belongs to clan GH-A and that the reaction catalyzed by Cel44A is retaining type hydrolysis. The stereochemical course of hydrolysis was confirmed by a (1)H NMR experiment using the reduced cellooligosaccharide as a substrate.

			Crystal structure of Cel44A, a glycoside hydrolase family 44 endoglucanase from Clostridium thermocellum.,Kitago Y, Karita S, Watanabe N, Kamiya M, Aizawa T, Sakka K, Tanaka I J Biol Chem. 2007 Dec 7;282(49):35703-11. Epub 2007 Sep 28. PMID:17905739<ref>PMID:17905739</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 2eo7" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Glucanase 3D structures\|Glucanase 3D structures]]		*[[Glucanase 3D structures\|Glucanase 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Acetivibrio thermocellus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Karita S]]		[[Category: Karita S]]

OCA at 10:36, 17 February 2021

2021-02-17T10:36:26Z

← Older revision		Revision as of 10:36, 17 February 2021
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	==Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum==		==Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum==
	<StructureSection load='2eo7' size='340' side='right' caption='[[2eo7]]~~, [[Resolution\|resolution]] 1.75Å~~' scene=''>		<StructureSection load='2eo7' size='340' side='right'caption='[[2eo7]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>~~[[2eo7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"ruminiclostridium_thermocellum"_yutin_and_galperin_2013 "ruminiclostridium thermocellum" yutin and galperin 2013].~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EO7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2EO7 FirstGlance]. <br>		<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EO7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EO7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eo7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eo7 OCA], [https://pdbe.org/2eo7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eo7 RCSB], [https://www.ebi.ac.uk/pdbsum/2eo7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eo7 ProSAT]</span></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2e4t\|2e4t]], [[2e0p\|2e0p]], [[2ej1\|2ej1]], [[2eex\|2eex]]</td></tr>~~
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CelJ, CelJ-Cel44A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1515 "Ruminiclostridium thermocellum" Yutin and Galperin 2013])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2eo7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eo7 OCA], [~~http~~://pdbe.org/2eo7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2eo7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2eo7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2eo7 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eo7 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eo7 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The crystal structure of Cel44A, which is one of the enzymatic components of the cellulosome of Clostridium thermocellum, was solved at a resolution of 0.96 A. This enzyme belongs to glycoside hydrolase family (GH family) 44. The structure reveals that Cel44A consists of a TIM-like barrel domain and a beta-sandwich domain. The wild-type and the E186Q mutant structures complexed with substrates suggest that two glutamic acid residues, Glu(186) and Glu(359), are the active residues of the enzyme. Biochemical experiments were performed to confirm this idea. The structural features indicate that GH family 44 belongs to clan GH-A and that the reaction catalyzed by Cel44A is retaining type hydrolysis. The stereochemical course of hydrolysis was confirmed by a (1)H NMR experiment using the reduced cellooligosaccharide as a substrate.

	Crystal structure of Cel44A, a glycoside hydrolase family 44 endoglucanase from Clostridium thermocellum.,Kitago Y, Karita S, Watanabe N, Kamiya M, Aizawa T, Sakka K, Tanaka I J Biol Chem. 2007 Dec 7;282(49):35703-11. Epub 2007 Sep 28. PMID:17905739<ref>PMID:17905739</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2eo7" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Glucanase\|Glucanase]]		*[[Glucanase 3D structures\|Glucanase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Ruminiclostridium thermocellum yutin and galperin 2013~~]]		[[Category: Large Structures]]
	[[Category: Karita, S]]		[[Category: Karita S]]
	[[Category: Kitao, Y]]		[[Category: Kitao Y]]
	[[Category: Sakka, K]]		[[Category: Sakka K]]
	[[Category: Tanaka, I]]		[[Category: Tanaka I]]
	[[Category: Watanabe, N]]		[[Category: Watanabe N]]
	~~[[Category: Beta-sandwich]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: The composite domain of gh family 5]]~~
	~~[[Category: Tim-barrel]]~~
	~~[[Category: Tim-like barrel~~]]

OCA at 07:00, 6 June 2018

2018-06-06T07:00:23Z

← Older revision		Revision as of 07:00, 6 June 2018
Line 1:		Line 1:

	==Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum==		==Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum==
	<StructureSection load='2eo7' size='340' side='right' caption='[[2eo7]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='2eo7' size='340' side='right' caption='[[2eo7]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2eo7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Atcc_27405 Atcc 27405~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EO7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EO7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2eo7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"ruminiclostridium_thermocellum"_yutin_and_galperin_2013 "ruminiclostridium thermocellum" yutin and galperin 2013]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EO7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EO7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2e4t\|2e4t]], [[2e0p\|2e0p]], [[2ej1\|2ej1]], [[2eex\|2eex]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2e4t\|2e4t]], [[2e0p\|2e0p]], [[2ej1\|2ej1]], [[2eex\|2eex]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CelJ, CelJ-Cel44A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1515 ~~ATCC 27405~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CelJ, CelJ-Cel44A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1515 "Ruminiclostridium thermocellum" Yutin and Galperin 2013])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eo7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eo7 OCA], [http://pdbe.org/2eo7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2eo7 RCSB], [http://www.ebi.ac.uk/pdbsum/2eo7 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eo7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eo7 OCA], [http://pdbe.org/2eo7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2eo7 RCSB], [http://www.ebi.ac.uk/pdbsum/2eo7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2eo7 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eo/2eo7_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eo/2eo7_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eo7 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Atcc 27405~~]]		[[Category: Ruminiclostridium thermocellum yutin and galperin 2013]]
	[[Category: Karita, S]]		[[Category: Karita, S]]
	[[Category: Kitao, Y]]		[[Category: Kitao, Y]]

OCA at 17:49, 11 September 2015

2015-09-11T17:49:08Z

← Older revision		Revision as of 17:49, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2eo7' size='340' side='right' caption='[[2eo7]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='2eo7' size='340' side='right' caption='[[2eo7]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2eo7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Clostridium_thermocellum Clostridium thermocellum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EO7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EO7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2eo7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_27405 Atcc 27405]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EO7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EO7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2e4t\|2e4t]], [[2e0p\|2e0p]], [[2ej1\|2ej1]], [[2eex\|2eex]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2e4t\|2e4t]], [[2e0p\|2e0p]], [[2ej1\|2ej1]], [[2eex\|2eex]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CelJ, CelJ-Cel44A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1515 ~~Clostridium thermocellum~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CelJ, CelJ-Cel44A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1515 ATCC 27405])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eo7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eo7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2eo7 RCSB], [http://www.ebi.ac.uk/pdbsum/2eo7 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eo7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eo7 OCA], [http://pdbe.org/2eo7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2eo7 RCSB], [http://www.ebi.ac.uk/pdbsum/2eo7 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2eo7" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Clostridium thermocellum~~]]		[[Category: Atcc 27405]]
	[[Category: Karita, S]]		[[Category: Karita, S]]
	[[Category: Kitao, Y]]		[[Category: Kitao, Y]]

OCA at 18:15, 15 January 2015

2015-01-15T18:15:32Z

← Older revision		Revision as of 18:15, 15 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2eo7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EO7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EO7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2eo7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EO7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EO7 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2e4t\|2e4t]], [[2e0p\|2e0p]], [[2ej1\|2ej1]], [[2eex\|2eex]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2e4t\|2e4t]], [[2e0p\|2e0p]], [[2ej1\|2ej1]], [[2eex\|2eex]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CelJ, CelJ-Cel44A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1515 Clostridium thermocellum])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CelJ, CelJ-Cel44A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1515 Clostridium thermocellum])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eo7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eo7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2eo7 RCSB], [http://www.ebi.ac.uk/pdbsum/2eo7 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eo7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eo7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2eo7 RCSB], [http://www.ebi.ac.uk/pdbsum/2eo7 PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Clostridium thermocellum]]		[[Category: Clostridium thermocellum]]
	[[Category: Karita, S.]]		[[Category: Karita, S]]
	[[Category: Kitao, Y.]]		[[Category: Kitao, Y]]
	[[Category: Sakka, K.]]		[[Category: Sakka, K]]
	[[Category: Tanaka, I.]]		[[Category: Tanaka, I]]
	[[Category: Watanabe, N~~.]]~~		[[Category: Watanabe, N]]
	~~[[Category: 30]]~~
	~~[[Category: 39]]~~
	~~[[Category: 51~~]]
	[[Category: Beta-sandwich]]		[[Category: Beta-sandwich]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 03:06, 29 September 2014

2014-09-29T03:06:07Z

← Older revision		Revision as of 03:06, 29 September 2014
Line 1:		Line 1:
	[[Image:~~2eo7~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum==
			<StructureSection load='2eo7' size='340' side='right' caption='[[2eo7]], [[Resolution\|resolution]] 1.75Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2eo7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EO7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EO7 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2e4t\|2e4t]], [[2e0p\|2e0p]], [[2ej1\|2ej1]], [[2eex\|2eex]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CelJ, CelJ-Cel44A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1515 Clostridium thermocellum])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eo7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eo7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2eo7 RCSB], [http://www.ebi.ac.uk/pdbsum/2eo7 PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eo/2eo7_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The crystal structure of Cel44A, which is one of the enzymatic components of the cellulosome of Clostridium thermocellum, was solved at a resolution of 0.96 A. This enzyme belongs to glycoside hydrolase family (GH family) 44. The structure reveals that Cel44A consists of a TIM-like barrel domain and a beta-sandwich domain. The wild-type and the E186Q mutant structures complexed with substrates suggest that two glutamic acid residues, Glu(186) and Glu(359), are the active residues of the enzyme. Biochemical experiments were performed to confirm this idea. The structural features indicate that GH family 44 belongs to clan GH-A and that the reaction catalyzed by Cel44A is retaining type hydrolysis. The stereochemical course of hydrolysis was confirmed by a (1)H NMR experiment using the reduced cellooligosaccharide as a substrate.

	~~{{STRUCTURE_2eo7\| PDB=2eo7 \| SCENE= }}~~		Crystal structure of Cel44A, a glycoside hydrolase family 44 endoglucanase from Clostridium thermocellum.,Kitago Y, Karita S, Watanabe N, Kamiya M, Aizawa T, Sakka K, Tanaka I J Biol Chem. 2007 Dec 7;282(49):35703-11. Epub 2007 Sep 28. PMID:17905739<ref>PMID:17905739</ref>

	~~===Crystal structure of Cel44A~~, ~~GH family 44 endoglucanase from Clostridium thermocellum===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_17905739}}~~

	~~==About this Structure==~~
	~~[[2eo7]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Clostridium_thermocellum Clostridium thermocellum]~~. ~~Full crystallographic information is available from [http://oca.weizmann.ac.il~~/~~oca-bin/ocashort?id=2EO7 OCA].~~

	==See Also==		==See Also==
	*[[Glucanase\|Glucanase]]		*[[Glucanase\|Glucanase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:017905739<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Clostridium thermocellum]]		[[Category: Clostridium thermocellum]]
	[[Category: Karita, S.]]		[[Category: Karita, S.]]

OCA at 20:44, 20 October 2012

2012-10-20T20:44:24Z

← Older revision		Revision as of 20:44, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[2eo7]] is a 1 chain structure ~~of [[Glucanase]]~~ with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EO7 OCA].		[[2eo7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EO7 OCA].

	==See Also==		==See Also==

OCA: Protected "2eo7" [edit=sysop:move=sysop]

2012-07-27T11:27:54Z

Protected "2eo7" [edit=sysop:move=sysop]

← Older revision	Revision as of 11:27, 27 July 2012
(No difference)

OCA at 11:27, 27 July 2012

2012-07-27T11:27:52Z

← Older revision		Revision as of 11:27, 27 July 2012
Line 1:		Line 1:
	~~{{Seed}}~~
	[[Image:2eo7.png\|left\|200px]]		[[Image:2eo7.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2eo7", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2eo7\| PDB=2eo7 \| SCENE= }}		{{STRUCTURE_2eo7\| PDB=2eo7 \| SCENE= }}

	===Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum===		===Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_17905739}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 17905739 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_17905739}}		{{ABSTRACT_PUBMED_17905739}}

	==About this Structure==		==About this Structure==
	~~2EO7~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EO7 OCA].		[[2eo7]] is a 1 chain structure of [[Glucanase]] with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EO7 OCA].

			==See Also==
			*[[Glucanase\|Glucanase]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~17905739~~</ref><references group="xtra"/>		<ref group="xtra">PMID:017905739</ref><references group="xtra"/>
	[[Category: Clostridium thermocellum]]		[[Category: Clostridium thermocellum]]
	[[Category: Karita, S.]]		[[Category: Karita, S.]]
Line 38:		Line 29:
	[[Category: Tim-barrel]]		[[Category: Tim-barrel]]
	[[Category: Tim-like barrel]]		[[Category: Tim-like barrel]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 22 20:54:54 2009''~~

OCA at 17:54, 22 July 2009

2009-07-22T17:54:56Z

← Older revision		Revision as of 17:54, 22 July 2009
Line 12:		Line 12:
	===Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum===		===Crystal structure of Cel44A, GH family 44 endoglucanase from Clostridium thermocellum===


			<!--
			The line below this paragraph, {{ABSTRACT_PUBMED_17905739}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 17905739 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_17905739}}

	==About this Structure==		==About this Structure==
	2EO7 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EO7 OCA].		2EO7 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EO7 OCA].

			==Reference==
			<ref group="xtra">PMID:17905739</ref><references group="xtra"/>
	[[Category: Clostridium thermocellum]]		[[Category: Clostridium thermocellum]]
	[[Category: Karita, S.]]		[[Category: Karita, S.]]
Line 30:		Line 39:
	[[Category: Tim-like barrel]]		[[Category: Tim-like barrel]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Feb 18 05~~:09:09 2009''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 22 20:54:54 2009''