2epl - Revision history

OCA at 13:50, 13 March 2024

2024-03-13T13:50:04Z

← Older revision		Revision as of 13:50, 13 March 2024
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	<StructureSection load='2epl' size='340' side='right'caption='[[2epl]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='2epl' size='340' side='right'caption='[[2epl]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2epl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_10558 Atcc 10558~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EPL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EPL FirstGlance]. <br>		<table><tr><td colspan='2'>[[2epl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_gordonii Streptococcus gordonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EPL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EPL FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4Å</td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em~~;~~'>[[2epk\|2epk]], [[2epm\|2epm]], [[2epn\|2epn]], [[2epo\|2epo]]</div>~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>gcna ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1302 ATCC 10558])</td~~><~~/tr>~~
	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Beta-N-acetylhexosaminidase Beta-N-acetylhexosaminidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.52 3.2.1.52]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2epl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2epl OCA], [https://pdbe.org/2epl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2epl RCSB], [https://www.ebi.ac.uk/pdbsum/2epl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2epl ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2epl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2epl OCA], [https://pdbe.org/2epl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2epl RCSB], [https://www.ebi.ac.uk/pdbsum/2epl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2epl ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/Q6ST21_STRGN Q6ST21_STRGN]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2epl ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2epl ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The crystal structure of GcnA, an N-acetyl-beta-D-glucosaminidase from Streptococcus gordonii, was solved by multiple wavelength anomalous dispersion phasing using crystals of selenomethionine-substituted protein. GcnA is a homodimer with subunits each comprised of three domains. The structure of the C-terminal alpha-helical domain has not been observed previously and forms a large dimerisation interface. The fold of the N-terminal domain is observed in all structurally related glycosidases although its function is unknown. The central domain has a canonical (beta/alpha)(8) TIM-barrel fold which harbours the active site. The primary sequence and structure of this central domain identifies the enzyme as a family 20 glycosidase. Key residues implicated in catalysis have different conformations in two different crystal forms, which probably represent active and inactive conformations of the enzyme. The catalytic mechanism for this class of glycoside hydrolase, where the substrate rather than the enzyme provides the cleavage-inducing nucleophile, has been confirmed by the structure of GcnA complexed with a putative reaction intermediate analogue, N-acetyl-beta-D-glucosamine-thiazoline. The catalytic mechanism is discussed in light of these and other family 20 structures.

	Structure of N-acetyl-beta-D-glucosaminidase (GcnA) from the endocarditis pathogen Streptococcus gordonii and its complex with the mechanism-based inhibitor NAG-thiazoline.,Langley DB, Harty DW, Jacques NA, Hunter N, Guss JM, Collyer CA J Mol Biol. 2008 Mar 14;377(1):104-16. Epub 2007 Sep 16. PMID:18237743<ref>PMID:18237743</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2epl" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Atcc 10558]]~~
	~~[[Category: Beta-N-acetylhexosaminidase]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Collyer, C A~~]]		[[Category: Streptococcus gordonii]]
	[[Category: ~~Guss, J M~~]]		[[Category: Collyer CA]]
	[[Category: ~~Harty, D W.S~~]]		[[Category: Guss JM]]
	[[Category: ~~Langley, D B~~]]		[[Category: Harty DWS]]
	[[Category: ~~Family 20]]~~		[[Category: Langley DB]]
	~~[[Category: Gcna]]~~
	~~[[Category: Glucosaminidase]]~~
	~~[[Category: Glycoside hydrolase]]~~
	~~[[Category: Hydrolase~~]]

OCA at 12:22, 10 February 2021

2021-02-10T12:22:32Z

← Older revision		Revision as of 12:22, 10 February 2021
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	==N-acetyl-B-D-glucosaminidase (GCNA) from Streptococcus gordonii==		==N-acetyl-B-D-glucosaminidase (GCNA) from Streptococcus gordonii==
	<StructureSection load='2epl' size='340' side='right' caption='[[2epl]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='2epl' size='340' side='right'caption='[[2epl]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2epl]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_10558 Atcc 10558]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EPL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2EPL FirstGlance]. <br>		<table><tr><td colspan='2'>[[2epl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_10558 Atcc 10558]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EPL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EPL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2epk\|2epk]], [[2epm\|2epm]], [[2epn\|2epn]], [[2epo\|2epo]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2epk\|2epk]], [[2epm\|2epm]], [[2epn\|2epn]], [[2epo\|2epo]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gcna ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1302 ATCC 10558])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gcna ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1302 ATCC 10558])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Beta-N-acetylhexosaminidase Beta-N-acetylhexosaminidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.52 3.2.1.52] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-N-acetylhexosaminidase Beta-N-acetylhexosaminidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.52 3.2.1.52] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2epl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2epl OCA], [~~http~~://pdbe.org/2epl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2epl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2epl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2epl ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2epl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2epl OCA], [https://pdbe.org/2epl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2epl RCSB], [https://www.ebi.ac.uk/pdbsum/2epl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2epl ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ep/2epl_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ep/2epl_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 35:		Line 35:
	[[Category: Atcc 10558]]		[[Category: Atcc 10558]]
	[[Category: Beta-N-acetylhexosaminidase]]		[[Category: Beta-N-acetylhexosaminidase]]
			[[Category: Large Structures]]
	[[Category: Collyer, C A]]		[[Category: Collyer, C A]]
	[[Category: Guss, J M]]		[[Category: Guss, J M]]

OCA at 08:33, 12 October 2017

2017-10-12T08:33:43Z

← Older revision		Revision as of 08:33, 12 October 2017
Line 1:		Line 1:

	==N-acetyl-B-D-glucosaminidase (GCNA) from Streptococcus gordonii==		==N-acetyl-B-D-glucosaminidase (GCNA) from Streptococcus gordonii==
	<StructureSection load='2epl' size='340' side='right' caption='[[2epl]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='2epl' size='340' side='right' caption='[[2epl]], [[Resolution\|resolution]] 1.40Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gcna ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1302 ATCC 10558])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gcna ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1302 ATCC 10558])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-N-acetylhexosaminidase Beta-N-acetylhexosaminidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.52 3.2.1.52] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-N-acetylhexosaminidase Beta-N-acetylhexosaminidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.52 3.2.1.52] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2epl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2epl OCA], [http://pdbe.org/2epl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2epl RCSB], [http://www.ebi.ac.uk/pdbsum/2epl PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2epl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2epl OCA], [http://pdbe.org/2epl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2epl RCSB], [http://www.ebi.ac.uk/pdbsum/2epl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2epl ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 02:24, 7 February 2016

2016-02-07T02:24:41Z

← Older revision		Revision as of 02:24, 7 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2epl ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 20:47, 9 September 2015

2015-09-09T20:47:05Z

← Older revision		Revision as of 20:47, 9 September 2015
Line 2:		Line 2:
	<StructureSection load='2epl' size='340' side='right' caption='[[2epl]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='2epl' size='340' side='right' caption='[[2epl]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2epl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptococcus_gordonii Streptococcus gordonii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EPL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EPL FirstGlance]. <br>		<table><tr><td colspan='2'>[[2epl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_10558 Atcc 10558]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EPL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EPL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2epk\|2epk]], [[2epm\|2epm]], [[2epn\|2epn]], [[2epo\|2epo]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2epk\|2epk]], [[2epm\|2epm]], [[2epn\|2epn]], [[2epo\|2epo]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gcna ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1302 ~~Streptococcus gordonii~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gcna ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1302 ATCC 10558])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-N-acetylhexosaminidase Beta-N-acetylhexosaminidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.52 3.2.1.52] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-N-acetylhexosaminidase Beta-N-acetylhexosaminidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.52 3.2.1.52] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2epl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2epl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2epl RCSB], [http://www.ebi.ac.uk/pdbsum/2epl PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2epl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2epl OCA], [http://pdbe.org/2epl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2epl RCSB], [http://www.ebi.ac.uk/pdbsum/2epl PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2epl" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Atcc 10558]]
	[[Category: Beta-N-acetylhexosaminidase]]		[[Category: Beta-N-acetylhexosaminidase]]
	~~[[Category: Streptococcus gordonii]]~~
	[[Category: Collyer, C A]]		[[Category: Collyer, C A]]
	[[Category: Guss, J M]]		[[Category: Guss, J M]]

OCA at 16:27, 15 January 2015

2015-01-15T16:27:55Z

← Older revision		Revision as of 16:27, 15 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2epl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_gordonii Streptococcus gordonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EPL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EPL FirstGlance]. <br>		<table><tr><td colspan='2'>[[2epl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_gordonii Streptococcus gordonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EPL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EPL FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2epk\|2epk]], [[2epm\|2epm]], [[2epn\|2epn]], [[2epo\|2epo]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2epk\|2epk]], [[2epm\|2epm]], [[2epn\|2epn]], [[2epo\|2epo]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gcna ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1302 Streptococcus gordonii])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gcna ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1302 Streptococcus gordonii])</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-N-acetylhexosaminidase Beta-N-acetylhexosaminidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.52 3.2.1.52] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-N-acetylhexosaminidase Beta-N-acetylhexosaminidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.52 3.2.1.52] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2epl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2epl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2epl RCSB], [http://www.ebi.ac.uk/pdbsum/2epl PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2epl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2epl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2epl RCSB], [http://www.ebi.ac.uk/pdbsum/2epl PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	[[Category: Beta-N-acetylhexosaminidase]]		[[Category: Beta-N-acetylhexosaminidase]]
	[[Category: Streptococcus gordonii]]		[[Category: Streptococcus gordonii]]
	[[Category: Collyer, C A.]]		[[Category: Collyer, C A]]
	[[Category: Guss, J M.]]		[[Category: Guss, J M]]
	[[Category: Harty, D W.S.]]		[[Category: Harty, D W.S]]
	[[Category: Langley, D B.]]		[[Category: Langley, D B]]
	[[Category: Family 20]]		[[Category: Family 20]]
	[[Category: Gcna]]		[[Category: Gcna]]

OCA at 00:02, 30 September 2014

2014-09-30T00:02:30Z

← Older revision		Revision as of 00:02, 30 September 2014
Line 1:		Line 1:
	[[Image:~~2epl~~.~~png~~\|~~left~~\|~~200px~~]]		==N-acetyl-B-D-glucosaminidase (GCNA) from Streptococcus gordonii==
			<StructureSection load='2epl' size='340' side='right' caption='[[2epl]], [[Resolution\|resolution]] 1.40Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2epl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_gordonii Streptococcus gordonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EPL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EPL FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2epk\|2epk]], [[2epm\|2epm]], [[2epn\|2epn]], [[2epo\|2epo]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gcna ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1302 Streptococcus gordonii])</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-N-acetylhexosaminidase Beta-N-acetylhexosaminidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.52 3.2.1.52] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2epl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2epl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2epl RCSB], [http://www.ebi.ac.uk/pdbsum/2epl PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ep/2epl_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The crystal structure of GcnA, an N-acetyl-beta-D-glucosaminidase from Streptococcus gordonii, was solved by multiple wavelength anomalous dispersion phasing using crystals of selenomethionine-substituted protein. GcnA is a homodimer with subunits each comprised of three domains. The structure of the C-terminal alpha-helical domain has not been observed previously and forms a large dimerisation interface. The fold of the N-terminal domain is observed in all structurally related glycosidases although its function is unknown. The central domain has a canonical (beta/alpha)(8) TIM-barrel fold which harbours the active site. The primary sequence and structure of this central domain identifies the enzyme as a family 20 glycosidase. Key residues implicated in catalysis have different conformations in two different crystal forms, which probably represent active and inactive conformations of the enzyme. The catalytic mechanism for this class of glycoside hydrolase, where the substrate rather than the enzyme provides the cleavage-inducing nucleophile, has been confirmed by the structure of GcnA complexed with a putative reaction intermediate analogue, N-acetyl-beta-D-glucosamine-thiazoline. The catalytic mechanism is discussed in light of these and other family 20 structures.

	~~{{STRUCTURE_2epl\| PDB=2epl \| SCENE= }}~~		Structure of N-acetyl-beta-D-glucosaminidase (GcnA) from the endocarditis pathogen Streptococcus gordonii and its complex with the mechanism-based inhibitor NAG-thiazoline.,Langley DB, Harty DW, Jacques NA, Hunter N, Guss JM, Collyer CA J Mol Biol. 2008 Mar 14;377(1):104-16. Epub 2007 Sep 16. PMID:18237743<ref>PMID:18237743</ref>

	~~===N-acetyl-B-D-glucosaminidase (GCNA) from Streptococcus gordonii===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_18237743}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2epl]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptococcus_gordonii Streptococcus gordonii]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2EPL OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:018237743<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Beta-N-acetylhexosaminidase]]		[[Category: Beta-N-acetylhexosaminidase]]
	[[Category: Streptococcus gordonii]]		[[Category: Streptococcus gordonii]]

OCA: Protected "2epl" [edit=sysop:move=sysop]

2013-01-06T09:29:44Z

Protected "2epl" [edit=sysop:move=sysop]

← Older revision	Revision as of 09:29, 6 January 2013
(No difference)

OCA at 09:29, 6 January 2013

2013-01-06T09:29:39Z

← Older revision		Revision as of 09:29, 6 January 2013
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	~~{{Seed}}~~
	[[Image:2epl.png\|left\|200px]]		[[Image:2epl.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2epl", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2epl\| PDB=2epl \| SCENE= }}		{{STRUCTURE_2epl\| PDB=2epl \| SCENE= }}

	===N-acetyl-B-D-glucosaminidase (GCNA) from Streptococcus gordonii===		===N-acetyl-B-D-glucosaminidase (GCNA) from Streptococcus gordonii===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_18237743}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 18237743 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_18237743}}		{{ABSTRACT_PUBMED_18237743}}

	==About this Structure==		==About this Structure==
	~~2EPL~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_gordonii Streptococcus gordonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EPL OCA].		[[2epl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_gordonii Streptococcus gordonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EPL OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~18237743~~</ref><references group="xtra"/>		<ref group="xtra">PMID:018237743</ref><references group="xtra"/>
	[[Category: Beta-N-acetylhexosaminidase]]		[[Category: Beta-N-acetylhexosaminidase]]
	[[Category: Streptococcus gordonii]]		[[Category: Streptococcus gordonii]]
Line 34:		Line 22:
	[[Category: Glucosaminidase]]		[[Category: Glucosaminidase]]
	[[Category: Glycoside hydrolase]]		[[Category: Glycoside hydrolase]]
			[[Category: Hydrolase]]
	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Mon Feb 16 10:18:39 2009''~~

OCA at 08:18, 16 February 2009

2009-02-16T08:18:43Z

← Older revision		Revision as of 08:18, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
	2EPL is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_gordonii Streptococcus gordonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EPL OCA].		2EPL is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_gordonii Streptococcus gordonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EPL OCA].

	==Reference==		==Reference==
	Structure of N-acetyl-beta-D-glucosaminidase (GcnA) from the endocarditis pathogen Streptococcus gordonii and its complex with the mechanism-based inhibitor NAG-thiazoline., Langley DB, Harty DW, Jacques NA, Hunter N, Guss JM, Collyer CA, J Mol Biol. 2008 Mar 14;377(1):104-16. Epub 2007 Sep 16. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18237743 18237743]~~		<ref group="xtra">PMID:18237743</ref><references group="xtra"/>
	[[Category: Beta-N-acetylhexosaminidase]]		[[Category: Beta-N-acetylhexosaminidase]]
	~~[[Category: Single protein]]~~
	[[Category: Streptococcus gordonii]]		[[Category: Streptococcus gordonii]]
	[[Category: Collyer, C A.]]		[[Category: Collyer, C A.]]
Line 36:		Line 35:
	[[Category: Glycoside hydrolase]]		[[Category: Glycoside hydrolase]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun Jul 27 12~~:35:~~18 2008~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 10:18:39 2009''