2ev1 - Revision history

OCA at 13:50, 13 March 2024

2024-03-13T13:50:13Z

← Older revision		Revision as of 13:50, 13 March 2024
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	<StructureSection load='2ev1' size='340' side='right'caption='[[2ev1]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='2ev1' size='340' side='right'caption='[[2ev1]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ev1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus~~ tuberculosis~~" (zopf 1883) klein 1884~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EV1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EV1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ev1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EV1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EV1 FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>~~, ~~<scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em~~;~~'>[[1y10\|1y10]], [[1y11\|1y11]], [[2ev2\|2ev2]], [[2ev3\|2ev3]], [[2ev4\|2ev4]]</div>~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene></td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>Rv1264 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1773~~ "~~Bacillus tuberculosis~~" ~~(Zopf 1883) Klein 1884])</td~~><~~/tr>~~
	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ev1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ev1 OCA], [https://pdbe.org/2ev1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ev1 RCSB], [https://www.ebi.ac.uk/pdbsum/2ev1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ev1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ev1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ev1 OCA], [https://pdbe.org/2ev1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ev1 RCSB], [https://www.ebi.ac.uk/pdbsum/2ev1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ev1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/Y1264_MYCTU Y1264_MYCTU]] Catalyzes the formation of the second messenger cAMP.		[https://www.uniprot.org/uniprot/Y1264_MYCTU Y1264_MYCTU] Catalyzes the formation of the second messenger cAMP.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ev1 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ev1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The universal secondary messenger cAMP is produced by adenylyl cyclases (ACs). Most bacterial and all eukaryotic ACs belong to class III of six divergent classes. A class III characteristic is formation of the catalytic pocket at a dimer interface and the presence of additional regulatory domains. Mycobacterium tuberculosis possesses 15 class III ACs, including Rv1264, which is activated at acidic pH due to pH-dependent structural transitions of the Rv1264 dimer. It has been shown by X-ray crystallography that the N-terminal regulatory and C-terminal catalytic domains of Rv1264 interact in completely different ways in the active and inhibited states. Here, we report an in-depth structural and functional analysis of the regulatory domain of Rv1264. The 1.6 A resolution crystal structure shows the protein in a tight, disk-shaped dimer, formed around a helical bundle, and involving a protein chain crossover. To understand pH regulation, we determined structures at acidic and basic pH values and employed structure-based mutagenesis in the holoenzyme to elucidate regulation using an AC activity assay. It has been shown that regulatory and catalytic domains must be linked in a single protein chain. The new studies demonstrate that the length of the linker segment is decisive for regulation. Several amino acids on the surface of the regulatory domain, when exchanged, altered the pH-dependence of AC activity. However, these residues are not conserved amongst a number of related ACs. The closely related mycobacterial Rv2212, but not Rv1264, is strongly activated by the addition of fatty acids. The structure resolved the presence of a deeply embedded fatty acid, characterised as oleic acid by mass spectrometry, which may serve as a hinge. From these data, we conclude that the regulatory domain is a structural scaffold used for distinct regulatory purposes.

	The structure of the regulatory domain of the adenylyl cyclase Rv1264 from Mycobacterium tuberculosis with bound oleic acid.,Findeisen F, Linder JU, Schultz A, Schultz JE, Brugger B, Wieland F, Sinning I, Tews I J Mol Biol. 2007 Jun 22;369(5):1282-95. Epub 2007 Apr 12. PMID:17482646<ref>PMID:17482646</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2ev1" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[3D Adenylyl cyclase 3D structures\|3D Adenylyl cyclase 3D structures]]		*[[3D Adenylyl cyclase 3D structures\|3D Adenylyl cyclase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Adenylate cyclase]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Findeisen, F~~]]		[[Category: Mycobacterium tuberculosis]]
	[[Category: ~~Sinning, I~~]]		[[Category: Findeisen F]]
	[[Category: ~~Tews,~~ I]]		[[Category: Sinning I]]
	[[Category: ~~Alpha-helical]]~~		[[Category: Tews I]]
	~~[[Category: Lyase]]~~
	~~[[Category: Oleic acid]]~~
	~~[[Category: Regulatory domain of adenylyl cyclase~~]]

OCA at 12:30, 10 February 2021

2021-02-10T12:30:31Z

← Older revision		Revision as of 12:30, 10 February 2021
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	==Structure of Rv1264N, the regulatory domain of the mycobacterial adenylyl cylcase Rv1264, at pH 6.0==		==Structure of Rv1264N, the regulatory domain of the mycobacterial adenylyl cylcase Rv1264, at pH 6.0==
	<StructureSection load='2ev1' size='340' side='right' caption='[[2ev1]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='2ev1' size='340' side='right'caption='[[2ev1]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ev1]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EV1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2EV1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ev1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EV1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EV1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y10\|1y10]], [[1y11\|1y11]], [[2ev2\|2ev2]], [[2ev3\|2ev3]], [[2ev4\|2ev4]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1y10\|1y10]], [[1y11\|1y11]], [[2ev2\|2ev2]], [[2ev3\|2ev3]], [[2ev4\|2ev4]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv1264 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv1264 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ev1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ev1 OCA], [~~http~~://pdbe.org/2ev1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ev1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ev1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ev1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ev1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ev1 OCA], [https://pdbe.org/2ev1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ev1 RCSB], [https://www.ebi.ac.uk/pdbsum/2ev1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ev1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/Y1264_MYCTU Y1264_MYCTU]] Catalyzes the formation of the second messenger cAMP.		[[https://www.uniprot.org/uniprot/Y1264_MYCTU Y1264_MYCTU]] Catalyzes the formation of the second messenger cAMP.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ev/2ev1_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ev/2ev1_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</div>		</div>
	<div class="pdbe-citations 2ev1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2ev1" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[3D Adenylyl cyclase 3D structures\|3D Adenylyl cyclase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Adenylate cyclase]]		[[Category: Adenylate cyclase]]
			[[Category: Large Structures]]
	[[Category: Findeisen, F]]		[[Category: Findeisen, F]]
	[[Category: Sinning, I]]		[[Category: Sinning, I]]

OCA at 07:29, 18 October 2017

2017-10-18T07:29:00Z

← Older revision		Revision as of 07:29, 18 October 2017
Line 1:		Line 1:

	==Structure of Rv1264N, the regulatory domain of the mycobacterial adenylyl cylcase Rv1264, at pH 6.0==		==Structure of Rv1264N, the regulatory domain of the mycobacterial adenylyl cylcase Rv1264, at pH 6.0==
	<StructureSection load='2ev1' size='340' side='right' caption='[[2ev1]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='2ev1' size='340' side='right' caption='[[2ev1]], [[Resolution\|resolution]] 1.60Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv1264 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv1264 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ev1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ev1 OCA], [http://pdbe.org/2ev1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ev1 RCSB], [http://www.ebi.ac.uk/pdbsum/2ev1 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ev1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ev1 OCA], [http://pdbe.org/2ev1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ev1 RCSB], [http://www.ebi.ac.uk/pdbsum/2ev1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ev1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 2ev1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2ev1" style="background-color:#fffaf0;"></div>

	~~==See Also==~~
	*[[Adenylyl cyclase\|Adenylyl cyclase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:23, 9 February 2016

2016-02-09T07:23:35Z

← Older revision		Revision as of 07:23, 9 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ev1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 19:56, 10 September 2015

2015-09-10T19:56:12Z

← Older revision		Revision as of 19:56, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2ev1' size='340' side='right' caption='[[2ev1]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='2ev1' size='340' side='right' caption='[[2ev1]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ev1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mycobacterium_tuberculosis Mycobacterium~~ tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EV1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EV1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ev1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EV1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EV1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y10\|1y10]], [[1y11\|1y11]], [[2ev2\|2ev2]], [[2ev3\|2ev3]], [[2ev4\|2ev4]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y10\|1y10]], [[1y11\|1y11]], [[2ev2\|2ev2]], [[2ev3\|2ev3]], [[2ev4\|2ev4]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv1264 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 ~~Mycobacterium~~ tuberculosis])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv1264 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ev1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ev1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ev1 RCSB], [http://www.ebi.ac.uk/pdbsum/2ev1 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ev1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ev1 OCA], [http://pdbe.org/2ev1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ev1 RCSB], [http://www.ebi.ac.uk/pdbsum/2ev1 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 29:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2ev1" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 37:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Adenylate cyclase]]		[[Category: Adenylate cyclase]]
	~~[[Category: Mycobacterium tuberculosis]]~~
	[[Category: Findeisen, F]]		[[Category: Findeisen, F]]
	[[Category: Sinning, I]]		[[Category: Sinning, I]]

OCA at 05:20, 25 December 2014

2014-12-25T05:20:05Z

← Older revision		Revision as of 05:20, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ev1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EV1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EV1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ev1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EV1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EV1 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y10\|1y10]], [[1y11\|1y11]], [[2ev2\|2ev2]], [[2ev3\|2ev3]], [[2ev4\|2ev4]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y10\|1y10]], [[1y11\|1y11]], [[2ev2\|2ev2]], [[2ev3\|2ev3]], [[2ev4\|2ev4]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv1264 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv1264 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ev1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ev1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ev1 RCSB], [http://www.ebi.ac.uk/pdbsum/2ev1 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ev1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ev1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ev1 RCSB], [http://www.ebi.ac.uk/pdbsum/2ev1 PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/Y1264_MYCTU Y1264_MYCTU]] Catalyzes the formation of the second messenger cAMP.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 38:
	[[Category: Adenylate cyclase]]		[[Category: Adenylate cyclase]]
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
	[[Category: Findeisen, F.]]		[[Category: Findeisen, F]]
	[[Category: Sinning, I.]]		[[Category: Sinning, I]]
	[[Category: Tews, I.]]		[[Category: Tews, I]]
	[[Category: Alpha-helical]]		[[Category: Alpha-helical]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Oleic acid]]		[[Category: Oleic acid]]
	[[Category: Regulatory domain of adenylyl cyclase]]		[[Category: Regulatory domain of adenylyl cyclase]]

OCA at 05:01, 29 September 2014

2014-09-29T05:01:52Z

← Older revision		Revision as of 05:01, 29 September 2014
Line 1:		Line 1:
	[[Image:~~2ev1~~.~~png~~\|~~left~~\|~~200px~~]]		==Structure of Rv1264N, the regulatory domain of the mycobacterial adenylyl cylcase Rv1264, at pH 6.0==
			<StructureSection load='2ev1' size='340' side='right' caption='[[2ev1]], [[Resolution\|resolution]] 1.60Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2ev1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EV1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EV1 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1y10\|1y10]], [[1y11\|1y11]], [[2ev2\|2ev2]], [[2ev3\|2ev3]], [[2ev4\|2ev4]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv1264 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ev1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ev1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ev1 RCSB], [http://www.ebi.ac.uk/pdbsum/2ev1 PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ev/2ev1_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The universal secondary messenger cAMP is produced by adenylyl cyclases (ACs). Most bacterial and all eukaryotic ACs belong to class III of six divergent classes. A class III characteristic is formation of the catalytic pocket at a dimer interface and the presence of additional regulatory domains. Mycobacterium tuberculosis possesses 15 class III ACs, including Rv1264, which is activated at acidic pH due to pH-dependent structural transitions of the Rv1264 dimer. It has been shown by X-ray crystallography that the N-terminal regulatory and C-terminal catalytic domains of Rv1264 interact in completely different ways in the active and inhibited states. Here, we report an in-depth structural and functional analysis of the regulatory domain of Rv1264. The 1.6 A resolution crystal structure shows the protein in a tight, disk-shaped dimer, formed around a helical bundle, and involving a protein chain crossover. To understand pH regulation, we determined structures at acidic and basic pH values and employed structure-based mutagenesis in the holoenzyme to elucidate regulation using an AC activity assay. It has been shown that regulatory and catalytic domains must be linked in a single protein chain. The new studies demonstrate that the length of the linker segment is decisive for regulation. Several amino acids on the surface of the regulatory domain, when exchanged, altered the pH-dependence of AC activity. However, these residues are not conserved amongst a number of related ACs. The closely related mycobacterial Rv2212, but not Rv1264, is strongly activated by the addition of fatty acids. The structure resolved the presence of a deeply embedded fatty acid, characterised as oleic acid by mass spectrometry, which may serve as a hinge. From these data, we conclude that the regulatory domain is a structural scaffold used for distinct regulatory purposes.

	~~{{STRUCTURE_2ev1\| PDB=2ev1 \| SCENE= }}~~		The structure of the regulatory domain of the adenylyl cyclase Rv1264 from Mycobacterium tuberculosis with bound oleic acid.,Findeisen F, Linder JU, Schultz A, Schultz JE, Brugger B, Wieland F, Sinning I, Tews I J Mol Biol. 2007 Jun 22;369(5):1282-95. Epub 2007 Apr 12. PMID:17482646<ref>PMID:17482646</ref>

	~~===Structure of Rv1264N~~, ~~the regulatory domain~~ of the ~~mycobacterial adenylyl cylcase Rv1264, at pH 6~~.~~0===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_17482646}}~~

	~~==About this Structure==~~
	~~[[2ev1]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il~~/~~oca-bin/ocashort?id=2EV1 OCA].~~

	==See Also==		==See Also==
	*[[Adenylyl cyclase\|Adenylyl cyclase]]		*[[Adenylyl cyclase\|Adenylyl cyclase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:017482646<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Adenylate cyclase]]		[[Category: Adenylate cyclase]]
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]

OCA at 13:06, 20 October 2012

2012-10-20T13:06:10Z

← Older revision		Revision as of 13:06, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[2ev1]] is a 2 chain structure ~~of [[Adenylyl cyclase]]~~ with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EV1 OCA].		[[2ev1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EV1 OCA].

	==See Also==		==See Also==

OCA at 10:54, 26 July 2012

2012-07-26T10:54:51Z

← Older revision		Revision as of 10:54, 26 July 2012
Line 1:		Line 1:
	[[Image:2ev1.png\|left\|200px]]		[[Image:2ev1.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2ev1", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2ev1\| PDB=2ev1 \| SCENE= }}		{{STRUCTURE_2ev1\| PDB=2ev1 \| SCENE= }}

	===Structure of Rv1264N, the regulatory domain of the mycobacterial adenylyl cylcase Rv1264, at pH 6.0===		===Structure of Rv1264N, the regulatory domain of the mycobacterial adenylyl cylcase Rv1264, at pH 6.0===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_17482646}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 17482646 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_17482646}}		{{ABSTRACT_PUBMED_17482646}}

Line 22:		Line 11:

	==See Also==		==See Also==
	*[[Adenylyl cyclase]]		*[[Adenylyl cyclase\|Adenylyl cyclase]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~17482646~~</ref><references group="xtra"/>		<ref group="xtra">PMID:017482646</ref><references group="xtra"/>
	[[Category: Adenylate cyclase]]		[[Category: Adenylate cyclase]]
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]

OCA: Protected "2ev1" [edit=sysop:move=sysop]

2010-12-27T01:04:53Z

Protected "2ev1" [edit=sysop:move=sysop]

← Older revision	Revision as of 01:04, 27 December 2010
(No difference)