2ev9 - Revision history

OCA at 08:43, 25 October 2023

2023-10-25T08:43:59Z

← Older revision		Revision as of 08:43, 25 October 2023
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	<StructureSection load='2ev9' size='340' side='right'caption='[[2ev9]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2ev9' size='340' side='right'caption='[[2ev9]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ev9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Thet8 Thet8~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EV9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EV9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ev9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EV9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EV9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SKM:(3R,4S,5R)-3,4,5-TRIHYDROXYCYCLOHEX-1-ENE-1-CARBOXYLIC+ACID'>SKM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1wxd\|1wxd]], [[2cy0\|2cy0]], [[2d5c\|2d5c]]</div></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SKM:(3R,4S,5R)-3,4,5-TRIHYDROXYCYCLOHEX-1-ENE-1-CARBOXYLIC+ACID'>SKM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AroE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>~~
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Shikimate_dehydrogenase Shikimate dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.25 1.1.1.25] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ev9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ev9 OCA], [https://pdbe.org/2ev9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ev9 RCSB], [https://www.ebi.ac.uk/pdbsum/2ev9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ev9 ProSAT], [https://www.topsan.org/Proteins/RSGI/2ev9 TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ev9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ev9 OCA], [https://pdbe.org/2ev9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ev9 RCSB], [https://www.ebi.ac.uk/pdbsum/2ev9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ev9 ProSAT], [https://www.topsan.org/Proteins/RSGI/2ev9 TOPSAN]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/AROE_THET8 AROE_THET8]] Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).[HAMAP-Rule:MF_00222]		[https://www.uniprot.org/uniprot/AROE_THET8 AROE_THET8] Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).[HAMAP-Rule:MF_00222]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	==See Also==		==See Also==
	*[[Shikimate dehydrogenase\|Shikimate dehydrogenase]]		*[[Shikimate dehydrogenase\|Shikimate dehydrogenase]]
			*[[Shikimate dehydrogenase 3D structures\|Shikimate dehydrogenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Shikimate dehydrogenase]]~~		[[Category: Thermus thermophilus HB8]]
	~~[[Category: Thet8~~]]		[[Category: Bagautdinov B]]
	[[Category: Bagautdinov, B]]		[[Category: Kunishima N]]
	[[Category: Kunishima, N]]
	~~[[Category: Structural genomic]]~~
	~~[[Category: Cofactor]]~~
	~~[[Category: Dimer]]~~
	~~[[Category: National project on protein structural and functional analyse]]~~
	~~[[Category: Nppsfa]]~~
	~~[[Category: Oxidoreductase]]~~
	~~[[Category: Rsgi]]~~
	~~[[Category: Shikimate]]~~
	~~[[Category: Substrate~~]]

OCA at 12:30, 10 February 2021

2021-02-10T12:30:57Z

← Older revision		Revision as of 12:30, 10 February 2021
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	==Crystal Structure of Shikimate 5-Dehydrogenase (AroE) from Thermus Thermophilus HB8 in complex with NADP(H) and shikimate==		==Crystal Structure of Shikimate 5-Dehydrogenase (AroE) from Thermus Thermophilus HB8 in complex with NADP(H) and shikimate==
	<StructureSection load='2ev9' size='340' side='right' caption='[[2ev9]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2ev9' size='340' side='right'caption='[[2ev9]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ev9]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EV9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2EV9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ev9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EV9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EV9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SKM:(3R,4S,5R)-3,4,5-TRIHYDROXYCYCLOHEX-1-ENE-1-CARBOXYLIC+ACID'>SKM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SKM:(3R,4S,5R)-3,4,5-TRIHYDROXYCYCLOHEX-1-ENE-1-CARBOXYLIC+ACID'>SKM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wxd\|1wxd]], [[2cy0\|2cy0]], [[2d5c\|2d5c]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1wxd\|1wxd]], [[2cy0\|2cy0]], [[2d5c\|2d5c]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AroE ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AroE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Shikimate_dehydrogenase Shikimate dehydrogenase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.25 1.1.1.25] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Shikimate_dehydrogenase Shikimate dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.25 1.1.1.25] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ev9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ev9 OCA], [~~http~~://pdbe.org/2ev9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ev9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ev9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ev9 ProSAT], [~~http~~://www.topsan.org/Proteins/RSGI/2ev9 TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ev9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ev9 OCA], [https://pdbe.org/2ev9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ev9 RCSB], [https://www.ebi.ac.uk/pdbsum/2ev9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ev9 ProSAT], [https://www.topsan.org/Proteins/RSGI/2ev9 TOPSAN]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/AROE_THET8 AROE_THET8]] Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).[HAMAP-Rule:MF_00222]		[[https://www.uniprot.org/uniprot/AROE_THET8 AROE_THET8]] Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).[HAMAP-Rule:MF_00222]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Shikimate dehydrogenase]]		[[Category: Shikimate dehydrogenase]]
	[[Category: Thet8]]		[[Category: Thet8]]

OCA at 09:13, 14 June 2018

2018-06-14T09:13:19Z

← Older revision		Revision as of 09:13, 14 June 2018
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	==Crystal Structure of Shikimate 5-Dehydrogenase (AroE) from Thermus Thermophilus HB8 in complex with NADP(H) and shikimate==		==Crystal Structure of Shikimate 5-Dehydrogenase (AroE) from Thermus Thermophilus HB8 in complex with NADP(H) and shikimate==
	<StructureSection load='2ev9' size='340' side='right' caption='[[2ev9]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2ev9' size='340' side='right' caption='[[2ev9]], [[Resolution\|resolution]] 1.90Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AroE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AroE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Shikimate_dehydrogenase Shikimate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.25 1.1.1.25] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Shikimate_dehydrogenase Shikimate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.25 1.1.1.25] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ev9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ev9 OCA], [http://pdbe.org/2ev9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ev9 RCSB], [http://www.ebi.ac.uk/pdbsum/2ev9 PDBsum], [http://www.topsan.org/Proteins/RSGI/2ev9 TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ev9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ev9 OCA], [http://pdbe.org/2ev9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ev9 RCSB], [http://www.ebi.ac.uk/pdbsum/2ev9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ev9 ProSAT], [http://www.topsan.org/Proteins/RSGI/2ev9 TOPSAN]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ev/2ev9_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ev/2ev9_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 15:32, 7 February 2016

2016-02-07T15:32:47Z

← Older revision		Revision as of 15:32, 7 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ev9 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 02:49, 10 September 2015

2015-09-10T02:49:52Z

← Older revision		Revision as of 02:49, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2ev9' size='340' side='right' caption='[[2ev9]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2ev9' size='340' side='right' caption='[[2ev9]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ev9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermus_thermophilus_hb8 Thermus thermophilus hb8~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EV9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EV9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ev9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EV9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EV9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SKM:(3R,4S,5R)-3,4,5-TRIHYDROXYCYCLOHEX-1-ENE-1-CARBOXYLIC+ACID'>SKM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SKM:(3R,4S,5R)-3,4,5-TRIHYDROXYCYCLOHEX-1-ENE-1-CARBOXYLIC+ACID'>SKM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wxd\|1wxd]], [[2cy0\|2cy0]], [[2d5c\|2d5c]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wxd\|1wxd]], [[2cy0\|2cy0]], [[2d5c\|2d5c]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AroE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 ~~Thermus thermophilus HB8~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AroE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Shikimate_dehydrogenase Shikimate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.25 1.1.1.25] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Shikimate_dehydrogenase Shikimate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.25 1.1.1.25] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ev9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ev9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ev9 RCSB], [http://www.ebi.ac.uk/pdbsum/2ev9 PDBsum], [http://www.topsan.org/Proteins/RSGI/2ev9 TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ev9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ev9 OCA], [http://pdbe.org/2ev9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ev9 RCSB], [http://www.ebi.ac.uk/pdbsum/2ev9 PDBsum], [http://www.topsan.org/Proteins/RSGI/2ev9 TOPSAN]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/AROE_THET8 AROE_THET8]] Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).[HAMAP-Rule:MF_00222]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2ev9" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Shikimate dehydrogenase]]		[[Category: Shikimate dehydrogenase]]
	[[Category: ~~Thermus thermophilus hb8~~]]		[[Category: Thet8]]
	[[Category: Bagautdinov, B]]		[[Category: Bagautdinov, B]]
	[[Category: Kunishima, N]]		[[Category: Kunishima, N]]

OCA at 17:01, 15 January 2015

2015-01-15T17:01:45Z

← Older revision		Revision as of 17:01, 15 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ev9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EV9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EV9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ev9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EV9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EV9 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SKM:(3R,4S,5R)-3,4,5-TRIHYDROXYCYCLOHEX-1-ENE-1-CARBOXYLIC+ACID'>SKM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SKM:(3R,4S,5R)-3,4,5-TRIHYDROXYCYCLOHEX-1-ENE-1-CARBOXYLIC+ACID'>SKM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wxd\|1wxd]], [[2cy0\|2cy0]], [[2d5c\|2d5c]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wxd\|1wxd]], [[2cy0\|2cy0]], [[2d5c\|2d5c]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AroE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 Thermus thermophilus HB8])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AroE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 Thermus thermophilus HB8])</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Shikimate_dehydrogenase Shikimate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.25 1.1.1.25] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Shikimate_dehydrogenase Shikimate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.25 1.1.1.25] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ev9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ev9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ev9 RCSB], [http://www.ebi.ac.uk/pdbsum/2ev9 PDBsum], [http://www.topsan.org/Proteins/RSGI/2ev9 TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ev9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ev9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ev9 RCSB], [http://www.ebi.ac.uk/pdbsum/2ev9 PDBsum], [http://www.topsan.org/Proteins/RSGI/2ev9 TOPSAN]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	[[Category: Shikimate dehydrogenase]]		[[Category: Shikimate dehydrogenase]]
	[[Category: Thermus thermophilus hb8]]		[[Category: Thermus thermophilus hb8]]
	[[Category: Bagautdinov, B.]]		[[Category: Bagautdinov, B]]
	[[Category: Kunishima, N.]]		[[Category: Kunishima, N]]
	[[Category: ~~RSGI, RIKEN~~ Structural ~~Genomics/Proteomics Initiative.~~]]		[[Category: Structural genomic]]
	[[Category: Cofactor]]		[[Category: Cofactor]]
	[[Category: Dimer]]		[[Category: Dimer]]
Line 44:		Line 44:
	[[Category: Nppsfa]]		[[Category: Nppsfa]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	~~[[Category: Riken structural genomics/proteomics initiative]]~~
	[[Category: Rsgi]]		[[Category: Rsgi]]
	[[Category: Shikimate]]		[[Category: Shikimate]]
	~~[[Category: Structural genomic]]~~
	[[Category: Substrate]]		[[Category: Substrate]]

OCA at 02:05, 30 September 2014

2014-09-30T02:05:58Z

← Older revision		Revision as of 02:05, 30 September 2014
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	[[Image:~~2ev9~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal Structure of Shikimate 5-Dehydrogenase (AroE) from Thermus Thermophilus HB8 in complex with NADP(H) and shikimate==
			<StructureSection load='2ev9' size='340' side='right' caption='[[2ev9]], [[Resolution\|resolution]] 1.90Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2ev9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EV9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EV9 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SKM:(3R,4S,5R)-3,4,5-TRIHYDROXYCYCLOHEX-1-ENE-1-CARBOXYLIC+ACID'>SKM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wxd\|1wxd]], [[2cy0\|2cy0]], [[2d5c\|2d5c]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AroE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 Thermus thermophilus HB8])</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Shikimate_dehydrogenase Shikimate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.25 1.1.1.25] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ev9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ev9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ev9 RCSB], [http://www.ebi.ac.uk/pdbsum/2ev9 PDBsum], [http://www.topsan.org/Proteins/RSGI/2ev9 TOPSAN]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ev/2ev9_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Shikimate dehydrogenase (EC 1.1.1.25) catalyses the fourth step of the shikimate pathway which is required for the synthesis of the aromatic amino acids and other aromatic compounds in bacteria, microbial eukaryotes, and plants. The crystal structures of the shikimate dehydrogenase AroE from Thermus thermophilus HB8 in its ligand-free form, binary complexes with cofactor NADP+ or substrate shikimate, and the ternary complex with both NADP(H) and shikimate were determined by X-ray diffraction method at atomic resolutions. The crystals are nearly isomorphous with the asymmetric unit containing a dimer, each subunit of which has a bi-domain structure of compact alpha/beta sandwich folds. The two subunits of the enzyme display asymmetry in the crystals due to different relative orientations between the N- and C-terminal domains resulting in a slightly different closure of the interdomain clefts. NADP(H) is bound to the more closed form only. This closed conformation with apparent higher affinity to the cofactor is also observed in the unliganded crystal form, indicating that the NADP(H) binding to TtAroE may follow the selection mode where the cofactor binds to the subunit that happens to be in the closed conformation in solution. Crystal structures of the closed subunits with and without NADP(H) show no significant structural difference, suggesting that the cofactor binding to the closed subunit corresponds to the lock-and-key model in TtAroE. On the other hand, shikimate binds to both open and closed subunit conformers of both apo and NADP(H)-liganded holo enzyme forms. The ternary complex TtAroE:NADP(H):shikimate allows unambiguous visualization of the SDH permitting elucidation of the roles of conserved residues Lys64 and Asp100 in the hydride ion transfer between NADP(H) and shikimate.

	~~{{STRUCTURE_2ev9\| PDB=2ev9 \| SCENE= }}~~		Crystal structures of shikimate dehydrogenase AroE from Thermus thermophilus HB8 and its cofactor and substrate complexes: insights into the enzymatic mechanism.,Bagautdinov B, Kunishima N J Mol Biol. 2007 Oct 19;373(2):424-38. Epub 2007 Aug 21. PMID:17825835<ref>PMID:17825835</ref>

	~~===Crystal Structure~~ of ~~Shikimate 5-Dehydrogenase (AroE) from Thermus Thermophilus HB8 in complex with NADP(H) and shikimate===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>

	~~{{ABSTRACT_PUBMED_17825835}}~~		==See Also==
			*[[Shikimate dehydrogenase\|Shikimate dehydrogenase]]
	==~~About this Structure~~==		== References ==
	[[~~2ev9]~~] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EV9 OCA].		<references/>
			__TOC__
	==~~Reference~~==		</StructureSection>
	<~~ref group="xtra">PMID:017825835<~~/~~ref~~><~~ref group="xtra">PMID:017649975</ref><references group="xtra"~~/>
	[[Category: Shikimate dehydrogenase]]		[[Category: Shikimate dehydrogenase]]
	[[Category: Thermus thermophilus hb8]]		[[Category: Thermus thermophilus hb8]]

OCA: Protected "2ev9" [edit=sysop:move=sysop]

2013-01-06T11:18:48Z

Protected "2ev9" [edit=sysop:move=sysop]

← Older revision	Revision as of 11:18, 6 January 2013
(No difference)

OCA at 11:18, 6 January 2013

2013-01-06T11:18:46Z

← Older revision		Revision as of 11:18, 6 January 2013
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	~~{{Seed}}~~
	[[Image:2ev9.png\|left\|200px]]		[[Image:2ev9.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2ev9", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2ev9\| PDB=2ev9 \| SCENE= }}		{{STRUCTURE_2ev9\| PDB=2ev9 \| SCENE= }}

	===Crystal Structure of Shikimate 5-Dehydrogenase (AroE) from Thermus Thermophilus HB8 in complex with NADP(H) and shikimate===		===Crystal Structure of Shikimate 5-Dehydrogenase (AroE) from Thermus Thermophilus HB8 in complex with NADP(H) and shikimate===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_17825835}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 17825835 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_17825835}}		{{ABSTRACT_PUBMED_17825835}}

	==About this Structure==		==About this Structure==
	~~2EV9~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/~~Thermus_thermophilus~~ Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EV9 OCA].		[[2ev9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EV9 OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~17825835~~</ref><references group="xtra"/>		<ref group="xtra">PMID:017825835</ref><ref group="xtra">PMID:017649975</ref><references group="xtra"/>
	[[Category: Shikimate dehydrogenase]]		[[Category: Shikimate dehydrogenase]]
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus hb8]]
	[[Category: Bagautdinov, B.]]		[[Category: Bagautdinov, B.]]
	[[Category: Kunishima, N.]]		[[Category: Kunishima, N.]]
Line 39:		Line 27:
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	[[Category: Substrate]]		[[Category: Substrate]]
	~~[[Category: X-ray diffraction]]~~

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 14:48:16 2009''~~

OCA at 12:48, 16 February 2009

2009-02-16T12:48:17Z

← Older revision		Revision as of 12:48, 16 February 2009
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	==Reference==		==Reference==
	Crystal structures of shikimate dehydrogenase AroE from Thermus thermophilus HB8 and its cofactor and substrate complexes: insights into the enzymatic mechanism., Bagautdinov B, Kunishima N, J Mol Biol. 2007 Oct 19;373(2):424-38. Epub 2007 Aug 21. PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~17825835 17825835]~~		<ref group="xtra">PMID:17825835</ref><references group="xtra"/>
	[[Category: Shikimate dehydrogenase]]		[[Category: Shikimate dehydrogenase]]
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]
Line 41:		Line 41:
	[[Category: X-ray diffraction]]		[[Category: X-ray diffraction]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun Nov~~ 16 17:42:~~11 2008~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 14:48:16 2009''