2f4f - Revision history

OCA at 13:50, 13 March 2024

2024-03-13T13:50:25Z

← Older revision		Revision as of 13:50, 13 March 2024
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	<StructureSection load='2f4f' size='340' side='right'caption='[[2f4f]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2f4f' size='340' side='right'caption='[[2f4f]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2f4f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~'saccharolobus_solfataricus' 'saccharolobus~~ solfataricus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F4F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F4F FirstGlance]. <br>		<table><tr><td colspan='2'>[[2f4f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F4F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F4F FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[2f5g\|2f5g]]~~</~~div~~></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f4f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f4f OCA], [https://pdbe.org/2f4f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f4f RCSB], [https://www.ebi.ac.uk/pdbsum/2f4f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f4f ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f4f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f4f OCA], [https://pdbe.org/2f4f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f4f RCSB], [https://www.ebi.ac.uk/pdbsum/2f4f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f4f ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/Q97Y68_SACS2 Q97Y68_SACS2]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f4f ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f4f ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	IS200 transposases, present in many bacteria and Archaea, appear to be distinct from other groups of transposases. To provide a structural basis for understanding the action of IS200 transposases, we have determined the crystal structure of the SSO1474 protein from Sulfolobus solfataricus, a member of the IS200 family, in both Mn(2+)-bound and Mn(2+)-free forms. Its monomer fold is distinct from other classes of structurally characterized transposases. Two monomers form a tight dimer by exchanging the C-terminal alpha-helix and by merging the two central beta-sheets into a large beta-sheet. Glu(55), His(62), and four water molecules provide the direct coordination sphere of the catalytically essential metal ion in the Mn(2+)-bound structure. His(16), Asp(59), and His(60) also play important roles in maintaining the metal binding site. The catalytic site is formed at the interface between monomers. The candidate nucleophile in the transposition mechanism, strictly conserved Tyr(121) coming from the other monomer, is turned away from the active site, suggesting that a conformational change is likely to occur during the catalytic cycle.

	Crystal structure of a metal ion-bound IS200 transposase.,Lee HH, Yoon JY, Kim HS, Kang JY, Kim KH, Kim DJ, Ha JY, Mikami B, Yoon HJ, Suh SW J Biol Chem. 2006 Feb 17;281(7):4261-6. Epub 2005 Dec 11. PMID:16340015<ref>PMID:16340015</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2f4f" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Saccharolobus solfataricus]]		[[Category: Saccharolobus solfataricus]]
	~~[[Category: Large Structures]]~~		[[Category: Kang JY]]
	[[Category: Kang~~, J Y~~]]		[[Category: Kim DJ]]
	[[Category: Kim~~, D J~~]]		[[Category: Kim HS]]
	[[Category: Kim~~, H S~~]]		[[Category: Kim KH]]
	[[Category: Kim~~, K H~~]]		[[Category: Lee HH]]
	[[Category: Lee~~, H H~~]]		[[Category: Suh SW]]
	[[Category: Suh~~, S W~~]]		[[Category: Yoon JY]]
	[[Category: Yoon~~, J Y]]~~
	~~[[Category: Gene regulation]]~~
	~~[[Category: Mn complex~~]]

OCA at 16:00, 22 December 2021

2021-12-22T16:00:52Z

← Older revision		Revision as of 16:00, 22 December 2021
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	<StructureSection load='2f4f' size='340' side='right'caption='[[2f4f]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2f4f' size='340' side='right'caption='[[2f4f]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2f4f]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/'saccharolobus_solfataricus' 'saccharolobus solfataricus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F4F OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2F4F FirstGlance]. <br>		<table><tr><td colspan='2'>[[2f4f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/'saccharolobus_solfataricus' 'saccharolobus solfataricus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F4F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F4F FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2f5g\|2f5g]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2f5g\|2f5g]]</div></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2f4f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f4f OCA], [~~http~~://pdbe.org/2f4f PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2f4f RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2f4f PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2f4f ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f4f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f4f OCA], [https://pdbe.org/2f4f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f4f RCSB], [https://www.ebi.ac.uk/pdbsum/2f4f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f4f ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 10:45, 18 March 2020

2020-03-18T10:45:30Z

← Older revision		Revision as of 10:45, 18 March 2020
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	==Crystal structure of IS200 transposase==		==Crystal structure of IS200 transposase==
	<StructureSection load='2f4f' size='340' side='right' caption='[[2f4f]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2f4f' size='340' side='right'caption='[[2f4f]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2f4f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/'saccharolobus_solfataricus' 'saccharolobus solfataricus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F4F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F4F FirstGlance]. <br>		<table><tr><td colspan='2'>[[2f4f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/'saccharolobus_solfataricus' 'saccharolobus solfataricus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F4F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F4F FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Saccharolobus solfataricus]]		[[Category: Saccharolobus solfataricus]]
			[[Category: Large Structures]]
	[[Category: Kang, J Y]]		[[Category: Kang, J Y]]
	[[Category: Kim, D J]]		[[Category: Kim, D J]]

OCA at 09:18, 14 June 2018

2018-06-14T09:18:18Z

← Older revision		Revision as of 09:18, 14 June 2018
Line 1:		Line 1:

	==Crystal structure of IS200 transposase==		==Crystal structure of IS200 transposase==
	<StructureSection load='2f4f' size='340' side='right' caption='[[2f4f]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2f4f' size='340' side='right' caption='[[2f4f]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2f4f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Atcc_35091 Atcc 35091~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F4F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F4F FirstGlance]. <br>		<table><tr><td colspan='2'>[[2f4f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/'saccharolobus_solfataricus' 'saccharolobus solfataricus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F4F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F4F FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2f5g\|2f5g]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2f5g\|2f5g]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f4f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f4f OCA], [http://pdbe.org/2f4f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2f4f RCSB], [http://www.ebi.ac.uk/pdbsum/2f4f PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f4f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f4f OCA], [http://pdbe.org/2f4f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2f4f RCSB], [http://www.ebi.ac.uk/pdbsum/2f4f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2f4f ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f4/2f4f_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f4/2f4f_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Atcc 35091~~]]		[[Category: Saccharolobus solfataricus]]
	[[Category: Kang, J Y]]		[[Category: Kang, J Y]]
	[[Category: Kim, D J]]		[[Category: Kim, D J]]

OCA at 12:09, 8 February 2016

2016-02-08T12:09:28Z

← Older revision		Revision as of 12:09, 8 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f4f ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 11:26, 10 September 2015

2015-09-10T11:26:29Z

← Older revision		Revision as of 11:26, 10 September 2015
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	<StructureSection load='2f4f' size='340' side='right' caption='[[2f4f]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2f4f' size='340' side='right' caption='[[2f4f]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2f4f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sulfolobus_solfataricus Sulfolobus solfataricus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F4F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F4F FirstGlance]. <br>		<table><tr><td colspan='2'>[[2f4f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F4F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F4F FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2f5g\|2f5g]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2f5g\|2f5g]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f4f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f4f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2f4f RCSB], [http://www.ebi.ac.uk/pdbsum/2f4f PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f4f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f4f OCA], [http://pdbe.org/2f4f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2f4f RCSB], [http://www.ebi.ac.uk/pdbsum/2f4f PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2f4f" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Sulfolobus solfataricus~~]]		[[Category: Atcc 35091]]
	[[Category: Kang, J Y]]		[[Category: Kang, J Y]]
	[[Category: Kim, D J]]		[[Category: Kim, D J]]

OCA at 09:27, 16 January 2015

2015-01-16T09:27:03Z

← Older revision		Revision as of 09:27, 16 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2f4f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F4F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F4F FirstGlance]. <br>		<table><tr><td colspan='2'>[[2f4f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F4F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F4F FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2f5g\|2f5g]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2f5g\|2f5g]]</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f4f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f4f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2f4f RCSB], [http://www.ebi.ac.uk/pdbsum/2f4f PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f4f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f4f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2f4f RCSB], [http://www.ebi.ac.uk/pdbsum/2f4f PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Sulfolobus solfataricus]]		[[Category: Sulfolobus solfataricus]]
	[[Category: Kang, J Y.]]		[[Category: Kang, J Y]]
	[[Category: Kim, D J.]]		[[Category: Kim, D J]]
	[[Category: Kim, H S.]]		[[Category: Kim, H S]]
	[[Category: Kim, K H.]]		[[Category: Kim, K H]]
	[[Category: Lee, H H.]]		[[Category: Lee, H H]]
	[[Category: Suh, S W.]]		[[Category: Suh, S W]]
	[[Category: Yoon, J Y.]]		[[Category: Yoon, J Y]]
	[[Category: Gene regulation]]		[[Category: Gene regulation]]
	[[Category: Mn complex]]		[[Category: Mn complex]]

OCA at 01:21, 30 September 2014

2014-09-30T01:21:53Z

← Older revision		Revision as of 01:21, 30 September 2014
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	[[Image:~~2f4f~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of IS200 transposase==
			<StructureSection load='2f4f' size='340' side='right' caption='[[2f4f]], [[Resolution\|resolution]] 1.80Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2f4f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F4F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F4F FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2f5g\|2f5g]]</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f4f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f4f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2f4f RCSB], [http://www.ebi.ac.uk/pdbsum/2f4f PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f4/2f4f_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			IS200 transposases, present in many bacteria and Archaea, appear to be distinct from other groups of transposases. To provide a structural basis for understanding the action of IS200 transposases, we have determined the crystal structure of the SSO1474 protein from Sulfolobus solfataricus, a member of the IS200 family, in both Mn(2+)-bound and Mn(2+)-free forms. Its monomer fold is distinct from other classes of structurally characterized transposases. Two monomers form a tight dimer by exchanging the C-terminal alpha-helix and by merging the two central beta-sheets into a large beta-sheet. Glu(55), His(62), and four water molecules provide the direct coordination sphere of the catalytically essential metal ion in the Mn(2+)-bound structure. His(16), Asp(59), and His(60) also play important roles in maintaining the metal binding site. The catalytic site is formed at the interface between monomers. The candidate nucleophile in the transposition mechanism, strictly conserved Tyr(121) coming from the other monomer, is turned away from the active site, suggesting that a conformational change is likely to occur during the catalytic cycle.

	~~{{STRUCTURE_2f4f\| PDB=2f4f \| SCENE= }}~~		Crystal structure of a metal ion-bound IS200 transposase.,Lee HH, Yoon JY, Kim HS, Kang JY, Kim KH, Kim DJ, Ha JY, Mikami B, Yoon HJ, Suh SW J Biol Chem. 2006 Feb 17;281(7):4261-6. Epub 2005 Dec 11. PMID:16340015<ref>PMID:16340015</ref>

	~~===Crystal structure~~ of ~~IS200 transposase===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_16340015}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2f4f]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2F4F OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:016340015<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Sulfolobus solfataricus]]		[[Category: Sulfolobus solfataricus]]
	[[Category: Kang, J Y.]]		[[Category: Kang, J Y.]]

OCA: Protected "2f4f" [edit=sysop:move=sysop]

2013-01-07T11:21:37Z

Protected "2f4f" [edit=sysop:move=sysop]

← Older revision	Revision as of 11:21, 7 January 2013
(No difference)

OCA at 11:21, 7 January 2013

2013-01-07T11:21:33Z

← Older revision		Revision as of 11:21, 7 January 2013
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	~~{{Seed}}~~
	[[Image:2f4f.png\|left\|200px]]		[[Image:2f4f.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2f4f", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2f4f\| PDB=2f4f \| SCENE= }}		{{STRUCTURE_2f4f\| PDB=2f4f \| SCENE= }}

	===Crystal structure of IS200 transposase===		===Crystal structure of IS200 transposase===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_16340015}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 16340015 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_16340015}}		{{ABSTRACT_PUBMED_16340015}}

	==About this Structure==		==About this Structure==
	~~2F4F~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F4F OCA].		[[2f4f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F4F OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~16340015~~</ref><references group="xtra"/>		<ref group="xtra">PMID:016340015</ref><references group="xtra"/>
	[[Category: Sulfolobus solfataricus]]		[[Category: Sulfolobus solfataricus]]
	[[Category: Kang, J Y.]]		[[Category: Kang, J Y.]]
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	[[Category: Suh, S W.]]		[[Category: Suh, S W.]]
	[[Category: Yoon, J Y.]]		[[Category: Yoon, J Y.]]
			[[Category: Gene regulation]]
	[[Category: Mn complex]]		[[Category: Mn complex]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 21:53:34 2009''~~