2f8f - Revision history

OCA at 09:22, 30 August 2023

2023-08-30T09:22:34Z

← Older revision		Revision as of 09:22, 30 August 2023
Line 3:		Line 3:
	<StructureSection load='2f8f' size='340' side='right'caption='[[2f8f]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2f8f' size='340' side='right'caption='[[2f8f]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2f8f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Blood_fluke Blood fluke~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F8F FirstGlance]. <br>		<table><tr><td colspan='2'>[[2f8f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schistosoma_haematobium Schistosoma haematobium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F8F FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18]~~ </~~span~~></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8f OCA], [https://pdbe.org/2f8f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f8f RCSB], [https://www.ebi.ac.uk/pdbsum/2f8f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f8f ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8f OCA], [https://pdbe.org/2f8f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f8f RCSB], [https://www.ebi.ac.uk/pdbsum/2f8f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f8f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/GST28_SCHBO GST28_SCHBO]] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.		[https://www.uniprot.org/uniprot/GST28_SCHBO GST28_SCHBO] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Blood fluke]]~~
	~~[[Category: Glutathione transferase]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Angelucci, F]]		[[Category: Schistosoma haematobium]]
	[[Category: Baiocco, P]]		[[Category: Angelucci F]]
	[[Category: Bellelli, A]]		[[Category: Baiocco P]]
	[[Category: Brunori, M]]		[[Category: Bellelli A]]
	[[Category: Gourlay~~, L J~~]]		[[Category: Brunori M]]
	[[Category: Miele~~, A E]]~~		[[Category: Gourlay LJ]]
	~~[[Category: Homodimer]]~~		[[Category: Miele AE]]
	~~[[Category: Protein_gtt complex]]~~
	~~[[Category: Thioredoxin fold]]~~
	~~[[Category: Transferase~~]]

OCA at 17:23, 20 October 2021

2021-10-20T17:23:25Z

← Older revision		Revision as of 17:23, 20 October 2021
Line 3:		Line 3:
	<StructureSection load='2f8f' size='340' side='right'caption='[[2f8f]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2f8f' size='340' side='right'caption='[[2f8f]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2f8f]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Blood_fluke Blood fluke]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8F OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2F8F FirstGlance]. <br>		<table><tr><td colspan='2'>[[2f8f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Blood_fluke Blood fluke]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F8F FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2f8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8f OCA], [~~http~~://pdbe.org/2f8f PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2f8f RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2f8f PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2f8f ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8f OCA], [https://pdbe.org/2f8f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f8f RCSB], [https://www.ebi.ac.uk/pdbsum/2f8f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f8f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/GST28_SCHBO GST28_SCHBO]] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.		[[https://www.uniprot.org/uniprot/GST28_SCHBO GST28_SCHBO]] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 10:46, 18 March 2020

2020-03-18T10:46:48Z

← Older revision		Revision as of 10:46, 18 March 2020
Line 1:		Line 1:

	==Crystal structure of the Y10F mutant of the gluathione s-transferase from schistosoma haematobium==		==Crystal structure of the Y10F mutant of the gluathione s-transferase from schistosoma haematobium==
	<StructureSection load='2f8f' size='340' side='right' caption='[[2f8f]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2f8f' size='340' side='right'caption='[[2f8f]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2f8f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Blood_fluke Blood fluke]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F8F FirstGlance]. <br>		<table><tr><td colspan='2'>[[2f8f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Blood_fluke Blood fluke]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F8F FirstGlance]. <br>
Line 31:		Line 31:

	==See Also==		==See Also==
	*[[Glutathione S-transferase\|Glutathione S-transferase]]		*[[Glutathione S-transferase 3D structures\|Glutathione S-transferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 38:		Line 38:
	[[Category: Blood fluke]]		[[Category: Blood fluke]]
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
			[[Category: Large Structures]]
	[[Category: Angelucci, F]]		[[Category: Angelucci, F]]
	[[Category: Baiocco, P]]		[[Category: Baiocco, P]]

OCA at 09:10, 14 June 2018

2018-06-14T09:10:15Z

← Older revision		Revision as of 09:10, 14 June 2018
Line 1:		Line 1:

	==Crystal structure of the Y10F mutant of the gluathione s-transferase from schistosoma haematobium==		==Crystal structure of the Y10F mutant of the gluathione s-transferase from schistosoma haematobium==
	<StructureSection load='2f8f' size='340' side='right' caption='[[2f8f]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2f8f' size='340' side='right' caption='[[2f8f]], [[Resolution\|resolution]] 2.10Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8f OCA], [http://pdbe.org/2f8f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2f8f RCSB], [http://www.ebi.ac.uk/pdbsum/2f8f PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8f OCA], [http://pdbe.org/2f8f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2f8f RCSB], [http://www.ebi.ac.uk/pdbsum/2f8f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2f8f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f8/2f8f_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f8/2f8f_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f8f ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:24, 11 September 2015

2015-09-11T09:24:23Z

← Older revision		Revision as of 09:24, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2f8f' size='340' side='right' caption='[[2f8f]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2f8f' size='340' side='right' caption='[[2f8f]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2f8f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Schistosoma_haematobium Schistosoma haematobium~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F8F FirstGlance]. <br>		<table><tr><td colspan='2'>[[2f8f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Blood_fluke Blood fluke]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F8F FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2f8f RCSB], [http://www.ebi.ac.uk/pdbsum/2f8f PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8f OCA], [http://pdbe.org/2f8f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2f8f RCSB], [http://www.ebi.ac.uk/pdbsum/2f8f PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2f8f" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Blood fluke]]
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
	~~[[Category: Schistosoma haematobium]]~~
	[[Category: Angelucci, F]]		[[Category: Angelucci, F]]
	[[Category: Baiocco, P]]		[[Category: Baiocco, P]]

OCA at 14:28, 25 December 2014

2014-12-25T14:28:01Z

← Older revision		Revision as of 14:28, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2f8f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Schistosoma_haematobium Schistosoma haematobium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F8F FirstGlance]. <br>		<table><tr><td colspan='2'>[[2f8f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Schistosoma_haematobium Schistosoma haematobium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F8F FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2f8f RCSB], [http://www.ebi.ac.uk/pdbsum/2f8f PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2f8f RCSB], [http://www.ebi.ac.uk/pdbsum/2f8f PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/GST28_SCHBO GST28_SCHBO]] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
	[[Category: Schistosoma haematobium]]		[[Category: Schistosoma haematobium]]
	[[Category: Angelucci, F.]]		[[Category: Angelucci, F]]
	[[Category: Baiocco, P.]]		[[Category: Baiocco, P]]
	[[Category: Bellelli, A.]]		[[Category: Bellelli, A]]
	[[Category: Brunori, M.]]		[[Category: Brunori, M]]
	[[Category: Gourlay, L J.]]		[[Category: Gourlay, L J]]
	[[Category: Miele, A E.]]		[[Category: Miele, A E]]
	[[Category: Homodimer]]		[[Category: Homodimer]]
	[[Category: Protein_gtt complex]]		[[Category: Protein_gtt complex]]
	[[Category: Thioredoxin fold]]		[[Category: Thioredoxin fold]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 11:19, 3 October 2014

2014-10-03T11:19:27Z

← Older revision		Revision as of 11:19, 3 October 2014
Line 1:		Line 1:
	~~{{STRUCTURE_2f8f\| PDB=2f8f \| SCENE= }}~~		==Crystal structure of the Y10F mutant of the gluathione s-transferase from schistosoma haematobium==
	===Crystal structure of the Y10F mutant of the gluathione s-transferase from schistosoma haematobium===		<StructureSection load='2f8f' size='340' side='right' caption='[[2f8f]], [[Resolution\|resolution]] 2.10Å' scene=''>
	~~{{ABSTRACT_PUBMED_16777141}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[2f8f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Schistosoma_haematobium Schistosoma haematobium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F8F FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene><br>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f8f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2f8f RCSB], [http://www.ebi.ac.uk/pdbsum/2f8f PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f8/2f8f_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			During turnover, the catalytic tyrosine residue (Tyr10) of the sigma class Schistosoma haematobium wild-type glutathione-S-transferase is expected to switch alternately in and out of the reduced glutathione-binding site (G-site). The Tyrout10 conformer forms a pi-cation interaction with the guanidinium group of Arg21. As in other similar glutathione-S-transferases, the catalytic Tyr has a low pKa of 7.2. In order to investigate the catalytic role of Tyr10, and the structural and functional roles of Arg21, we carried out structural studies on two Arg21 mutants (R21L and R21Q) and a Tyr10 mutant, Y10F. Our crystallographic data for the two Arg21 mutants indicate that only the Tyrout10 conformation is populated, thereby excluding a role of Arg21 in the stabilisation of the out conformation. However, Arg21 was confirmed to be catalytically important and essential for the low pKa of Tyr10. Upon comparison with structural data generated for reduced glutathione-bound and inhibitor-bound wild-type enzymes, it was observed that the orientations of Tyr10 and Arg35 are concerted and that, upon ligand binding, minor rearrangements occur within conserved residues in the active site loop. These rearrangements are coupled to quaternary rigid-body movements at the dimer interface and alterations in the localisation and structural order of the C-terminal domain.

	~~==Function==~~		Probing the mechanism of GSH activation in Schistosoma haematobium glutathione-S-transferase by site-directed mutagenesis and X-ray crystallography.,Baiocco P, Gourlay LJ, Angelucci F, Fontaine J, Herve M, Miele AE, Trottein F, Brunori M, Bellelli A J Mol Biol. 2006 Jul 14;360(3):678-89. Epub 2006 Jun 2. PMID:16777141<ref>PMID:16777141</ref>
	~~[[http://www.uniprot.org/uniprot/GST28_SCHBO GST28_SCHBO]] Conjugation~~ of ~~reduced~~ glutathione ~~to a wide number of exogenous~~ and ~~endogenous hydrophobic electrophiles~~. ~~GST isoenzymes appear to play a central role in the parasite detoxification system~~. ~~Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut~~.

	~~==About this Structure==~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~[[2f8f]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Schistosoma_haematobium Schistosoma haematobium]~~. ~~Full crystallographic information is available from [http:/~~/~~oca.weizmann.ac.il/oca-bin/ocashort?id=2F8F OCA].~~		</div>

	==See Also==		==See Also==
	*[[Glutathione S-transferase\|Glutathione S-transferase]]		*[[Glutathione S-transferase\|Glutathione S-transferase]]
			== References ==
	==~~Reference~~==		<references/>
	~~<ref group="xtra">PMID:016777141</ref>~~<references ~~group="xtra"~~/><~~references~~/>		__TOC__
			</StructureSection>
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
	[[Category: Schistosoma haematobium]]		[[Category: Schistosoma haematobium]]

OCA at 08:29, 23 April 2014

2014-04-23T08:29:06Z

← Older revision		Revision as of 08:29, 23 April 2014
Line 1:		Line 1:
	~~[[Image:2f8f.png\|left\|200px]]~~

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2f8f", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2f8f\| PDB=2f8f \| SCENE= }}		{{STRUCTURE_2f8f\| PDB=2f8f \| SCENE= }}

	===Crystal structure of the Y10F mutant of the gluathione s-transferase from schistosoma haematobium===		===Crystal structure of the Y10F mutant of the gluathione s-transferase from schistosoma haematobium===
			{{ABSTRACT_PUBMED_16777141}}

			==Function==
	~~<!--~~		[[http://www.uniprot.org/uniprot/GST28_SCHBO GST28_SCHBO]] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
	~~The line below this paragraph, {{ABSTRACT_PUBMED_16777141}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov), where 16777141 is~~ the ~~PubMed ID number~~.
	~~-->~~
	~~{{ABSTRACT_PUBMED_16777141}}~~

	==About this Structure==		==About this Structure==
	[[2f8f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Schistosoma_haematobium Schistosoma haematobium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8F OCA].		[[2f8f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Schistosoma_haematobium Schistosoma haematobium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8F OCA].

			==See Also==
			*[[Glutathione S-transferase\|Glutathione S-transferase]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:016777141</ref><references group="xtra"/>		<ref group="xtra">PMID:016777141</ref><references group="xtra"/><references/>
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
	[[Category: Schistosoma haematobium]]		[[Category: Schistosoma haematobium]]

OCA: Protected "2f8f" [edit=sysop:move=sysop]

2012-01-18T10:00:38Z

Protected "2f8f" [edit=sysop:move=sysop]

← Older revision	Revision as of 10:00, 18 January 2012
(No difference)

OCA at 10:00, 18 January 2012

2012-01-18T10:00:36Z

← Older revision		Revision as of 10:00, 18 January 2012
Line 1:		Line 1:
	~~{{Seed}}~~
	[[Image:2f8f.png\|left\|200px]]		[[Image:2f8f.png\|left\|200px]]

Line 20:		Line 19:

	==About this Structure==		==About this Structure==
	~~2F8F~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Schistosoma_haematobium Schistosoma haematobium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8F OCA].		[[2f8f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Schistosoma_haematobium Schistosoma haematobium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8F OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~16777141~~</ref><references group="xtra"/>		<ref group="xtra">PMID:016777141</ref><references group="xtra"/>
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
	[[Category: Schistosoma haematobium]]		[[Category: Schistosoma haematobium]]
Line 35:		Line 34:
	[[Category: Protein_gtt complex]]		[[Category: Protein_gtt complex]]
	[[Category: Thioredoxin fold]]		[[Category: Thioredoxin fold]]
			[[Category: Transferase]]
	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 18:42:01 2009''~~