2fc0 - Revision history

OCA at 09:24, 30 August 2023

2023-08-30T09:24:45Z

← Older revision		Revision as of 09:24, 30 August 2023
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	<StructureSection load='2fc0' size='340' side='right'caption='[[2fc0]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2fc0' size='340' side='right'caption='[[2fc0]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fc0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FC0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FC0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fc0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FC0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FC0 FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=DGP:2-DEOXYGUANOSINE~~-~~5-MONOPHOSPHATE'>DGP</scene>~~, ~~<scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[2fbt\|2fbt]], [[2fbv\|2fbv]], [[2fbx\|2fbx]]~~</~~div~~><~~/td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DGP:2-DEOXYGUANOSINE-5-MONOPHOSPHATE'>DGP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></~~td><td class="sblockDat"~~>~~WRN, RECQ3, RECQL2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fc0 OCA], [https://pdbe.org/2fc0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fc0 RCSB], [https://www.ebi.ac.uk/pdbsum/2fc0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fc0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fc0 OCA], [https://pdbe.org/2fc0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fc0 RCSB], [https://www.ebi.ac.uk/pdbsum/2fc0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fc0 ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
	[[https://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Defects in WRN are a cause of Werner syndrome (WRN) [MIM:[https://omim.org/entry/277700 277700]]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.<ref>PMID:16673358</ref> Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:[https://omim.org/entry/114500 114500]].		[https://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN] Defects in WRN are a cause of Werner syndrome (WRN) [MIM:[https://omim.org/entry/277700 277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.<ref>PMID:16673358</ref> Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:[https://omim.org/entry/114500 114500].
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity).<ref>PMID:11863428</ref> <ref>PMID:17563354</ref> <ref>PMID:18596042</ref> <ref>PMID:19652551</ref> <ref>PMID:19283071</ref>		[https://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN] Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity).<ref>PMID:11863428</ref> <ref>PMID:17563354</ref> <ref>PMID:18596042</ref> <ref>PMID:19652551</ref> <ref>PMID:19283071</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Human~~]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Perry~~, J J~~]]		[[Category: Perry JJ]]
	[[Category: Tainer~~, J A]]~~		[[Category: Tainer JA]]
	~~[[Category: 3'-5' exonuclease]]~~
	~~[[Category: Dnaq family]]~~
	~~[[Category: Recq]]~~
	~~[[Category: Transferase]]~~
	~~[[Category: Werner syndrome]]~~
	~~[[Category: Wrn~~]]

OCA at 07:14, 24 February 2021

2021-02-24T07:14:36Z

← Older revision		Revision as of 07:14, 24 February 2021
Line 1:		Line 1:

	==WRN exonuclease, Mn dGMP complex==		==WRN exonuclease, Mn dGMP complex==
	<StructureSection load='2fc0' size='340' side='right' caption='[[2fc0]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2fc0' size='340' side='right'caption='[[2fc0]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fc0]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FC0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2FC0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fc0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FC0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FC0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DGP:2-DEOXYGUANOSINE-5-MONOPHOSPHATE'>DGP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DGP:2-DEOXYGUANOSINE-5-MONOPHOSPHATE'>DGP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fbt\|2fbt]], [[2fbv\|2fbv]], [[2fbx\|2fbx]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2fbt\|2fbt]], [[2fbv\|2fbv]], [[2fbx\|2fbx]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WRN, RECQ3, RECQL2 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WRN, RECQ3, RECQL2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2fc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fc0 OCA], [~~http~~://pdbe.org/2fc0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2fc0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2fc0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2fc0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fc0 OCA], [https://pdbe.org/2fc0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fc0 RCSB], [https://www.ebi.ac.uk/pdbsum/2fc0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fc0 ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
	[[~~http~~://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Defects in WRN are a cause of Werner syndrome (WRN) [MIM:[~~http~~://omim.org/entry/277700 277700]]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.<ref>PMID:16673358</ref> Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:[~~http~~://omim.org/entry/114500 114500]].		[[https://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Defects in WRN are a cause of Werner syndrome (WRN) [MIM:[https://omim.org/entry/277700 277700]]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.<ref>PMID:16673358</ref> Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:[https://omim.org/entry/114500 114500]].
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity).<ref>PMID:11863428</ref> <ref>PMID:17563354</ref> <ref>PMID:18596042</ref> <ref>PMID:19652551</ref> <ref>PMID:19283071</ref>		[[https://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity).<ref>PMID:11863428</ref> <ref>PMID:17563354</ref> <ref>PMID:18596042</ref> <ref>PMID:19652551</ref> <ref>PMID:19283071</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	==See Also==		==See Also==
	*[[Helicase\|Helicase]]		*[[Helicase 3D structures\|Helicase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 40:		Line 40:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
			[[Category: Large Structures]]
	[[Category: Perry, J J]]		[[Category: Perry, J J]]
	[[Category: Tainer, J A]]		[[Category: Tainer, J A]]

OCA at 09:21, 14 June 2018

2018-06-14T09:21:19Z

← Older revision		Revision as of 09:21, 14 June 2018
Line 1:		Line 1:

	==WRN exonuclease, Mn dGMP complex==		==WRN exonuclease, Mn dGMP complex==
	<StructureSection load='2fc0' size='340' side='right' caption='[[2fc0]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2fc0' size='340' side='right' caption='[[2fc0]], [[Resolution\|resolution]] 2.00Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fbt\|2fbt]], [[2fbv\|2fbv]], [[2fbx\|2fbx]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fbt\|2fbt]], [[2fbv\|2fbv]], [[2fbx\|2fbx]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WRN, RECQ3, RECQL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WRN, RECQ3, RECQL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fc0 OCA], [http://pdbe.org/2fc0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fc0 RCSB], [http://www.ebi.ac.uk/pdbsum/2fc0 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fc0 OCA], [http://pdbe.org/2fc0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fc0 RCSB], [http://www.ebi.ac.uk/pdbsum/2fc0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2fc0 ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 16:		Line 17:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fc/2fc0_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fc/2fc0_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fc0 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 08:06, 11 September 2015

2015-09-11T08:06:10Z

← Older revision		Revision as of 08:06, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2fc0' size='340' side='right' caption='[[2fc0]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2fc0' size='340' side='right' caption='[[2fc0]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fc0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FC0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FC0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fc0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FC0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FC0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DGP:2-DEOXYGUANOSINE-5-MONOPHOSPHATE'>DGP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DGP:2-DEOXYGUANOSINE-5-MONOPHOSPHATE'>DGP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fbt\|2fbt]], [[2fbv\|2fbv]], [[2fbx\|2fbx]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fbt\|2fbt]], [[2fbv\|2fbv]], [[2fbx\|2fbx]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WRN, RECQ3, RECQL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WRN, RECQ3, RECQL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fc0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fc0 RCSB], [http://www.ebi.ac.uk/pdbsum/2fc0 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fc0 OCA], [http://pdbe.org/2fc0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fc0 RCSB], [http://www.ebi.ac.uk/pdbsum/2fc0 PDBsum]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 30:		Line 30:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2fc0" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 37:		Line 38:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Homo sapiens~~]]		[[Category: Human]]
	[[Category: Perry, J J]]		[[Category: Perry, J J]]
	[[Category: Tainer, J A]]		[[Category: Tainer, J A]]

OCA at 08:41, 16 January 2015

2015-01-16T08:41:06Z

← Older revision		Revision as of 08:41, 16 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fc0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FC0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FC0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fc0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FC0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FC0 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DGP:2-DEOXYGUANOSINE-5-MONOPHOSPHATE'>DGP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DGP:2-DEOXYGUANOSINE-5-MONOPHOSPHATE'>DGP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fbt\|2fbt]], [[2fbv\|2fbv]], [[2fbx\|2fbx]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fbt\|2fbt]], [[2fbv\|2fbv]], [[2fbx\|2fbx]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WRN, RECQ3, RECQL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WRN, RECQ3, RECQL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fc0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fc0 RCSB], [http://www.ebi.ac.uk/pdbsum/2fc0 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fc0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fc0 RCSB], [http://www.ebi.ac.uk/pdbsum/2fc0 PDBsum]</span></td></tr>
	<table>		</table>
	== Disease ==		== Disease ==
	[[http://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Defects in WRN are a cause of Werner syndrome (WRN) [MIM:[http://omim.org/entry/277700 277700]]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.<ref>PMID:16673358</ref> Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:[http://omim.org/entry/114500 114500]].		[[http://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Defects in WRN are a cause of Werner syndrome (WRN) [MIM:[http://omim.org/entry/277700 277700]]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.<ref>PMID:16673358</ref> Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:[http://omim.org/entry/114500 114500]].
Line 38:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Perry, J J.]]		[[Category: Perry, J J]]
	[[Category: Tainer, J A.]]		[[Category: Tainer, J A]]
	[[Category: 3'-5' exonuclease]]		[[Category: 3'-5' exonuclease]]
	[[Category: Dnaq family]]		[[Category: Dnaq family]]

OCA at 00:33, 30 September 2014

2014-09-30T00:33:27Z

← Older revision		Revision as of 00:33, 30 September 2014
Line 1:		Line 1:
	~~{{STRUCTURE_2fc0~~\| ~~PDB~~=2fc0 \| ~~SCENE~~= }}		==WRN exonuclease, Mn dGMP complex==
	===WRN exonuclease, ~~Mn dGMP complex~~===		<StructureSection load='2fc0' size='340' side='right' caption='[[2fc0]], [[Resolution\|resolution]] 2.00Å' scene=''>
	~~{{ABSTRACT_PUBMED_16622405}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[2fc0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FC0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FC0 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DGP:2-DEOXYGUANOSINE-5-MONOPHOSPHATE'>DGP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fbt\|2fbt]], [[2fbv\|2fbv]], [[2fbx\|2fbx]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WRN, RECQ3, RECQL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fc0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fc0 RCSB], [http://www.ebi.ac.uk/pdbsum/2fc0 PDBsum]</span></td></tr>
			<table>
			== Disease ==
			[[http://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Defects in WRN are a cause of Werner syndrome (WRN) [MIM:[http://omim.org/entry/277700 277700]]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.<ref>PMID:16673358</ref> Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:[http://omim.org/entry/114500 114500]].
			== Function ==
			[[http://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity).<ref>PMID:11863428</ref> <ref>PMID:17563354</ref> <ref>PMID:18596042</ref> <ref>PMID:19652551</ref> <ref>PMID:19283071</ref>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fc/2fc0_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			WRN is unique among the five human RecQ DNA helicases in having a functional exonuclease domain (WRN-exo) and being defective in the premature aging and cancer-related disorder Werner syndrome. Here, we characterize WRN-exo crystal structures, biochemical activity and participation in DNA end joining. Metal-ion complex structures, active site mutations and activity assays reveal a nuclease mechanism mediated by two metal ions. The DNA end-binding Ku70/80 complex specifically stimulates WRN-exo activity, and structure-based mutational inactivation of WRN-exo alters DNA end joining in human cells. We furthermore establish structural and biochemical similarities of WRN-exo to DnaQ-family replicative proofreading exonucleases, describing WRN-specific adaptations consistent with double-stranded DNA specificity and functionally important conformational changes. These results indicate WRN-exo is a human DnaQ family member and support DnaQ-like proofreading activities stimulated by Ku70/80, with implications for WRN functions in age-related pathologies and maintenance of genomic integrity.

	~~==Disease==~~		WRN exonuclease structure and molecular mechanism imply an editing role in DNA end processing.,Perry JJ, Yannone SM, Holden LG, Hitomi C, Asaithamby A, Han S, Cooper PK, Chen DJ, Tainer JA Nat Struct Mol Biol. 2006 May;13(5):414-22. Epub 2006 Apr 23. PMID:16622405<ref>PMID:16622405</ref>
	~~[[http://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Defects~~ in ~~WRN are a cause of Werner syndrome (WRN) [MIM:[http://omim~~.~~org/entry/277700 277700]]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders~~, ~~including atherosclerosis~~, ~~cancer~~, ~~non-insulin-dependent diabetes mellitus~~, ~~ocular cataracts and osteoporosis. The major cause of death~~, ~~at a median age of 47~~, ~~is myocardial infarction~~. ~~Currently all known WS mutations produces prematurely terminated proteins~~.<ref>PMID:~~16673358~~</ref> ~~Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:[http://omim.org/entry/114500 114500]].~~

	~~==Function==~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~[[http:~~/~~/www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with~~ a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of ~~DNA polymerases at~~ the ~~site of DNA lesions~~. ~~Important for genomic integrity~~. ~~Plays a role in the formation~~ of ~~DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity)~~.<~~ref~~>~~PMID:11863428</ref><ref>PMID:17563354~~</~~ref><ref~~>~~PMID:18596042</ref><ref>PMID:19652551</ref><ref>PMID:19283071</ref>~~		</div>

	~~==About this Structure==~~
	[[2fc0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FC0 OCA].

	==See Also==		==See Also==
	*[[Helicase\|Helicase]]		*[[Helicase\|Helicase]]
			== References ==
	==~~Reference~~==		<references/>
	~~<ref group="xtra">PMID:016622405</ref>~~<references ~~group="xtra"~~/><~~references~~/>		__TOC__
			</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Perry, J J.]]		[[Category: Perry, J J.]]

OCA at 04:04, 25 March 2013

2013-03-25T04:04:55Z

← Older revision		Revision as of 04:04, 25 March 2013
Line 1:		Line 1:
	~~[[Image:2fc0.png\|left\|200px]]~~

	{{STRUCTURE_2fc0\| PDB=2fc0 \| SCENE= }}		{{STRUCTURE_2fc0\| PDB=2fc0 \| SCENE= }}
			===WRN exonuclease, Mn dGMP complex===
			{{ABSTRACT_PUBMED_16622405}}

	===WRN ~~exonuclease~~, ~~Mn dGMP complex===~~		==Disease==
			[[http://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Defects in WRN are a cause of Werner syndrome (WRN) [MIM:[http://omim.org/entry/277700 277700]]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.<ref>PMID:16673358</ref> Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:[http://omim.org/entry/114500 114500]].

	~~{{ABSTRACT_PUBMED_16622405}}~~		==Function==
			[[http://www.uniprot.org/uniprot/WRN_HUMAN WRN_HUMAN]] Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity).<ref>PMID:11863428</ref><ref>PMID:17563354</ref><ref>PMID:18596042</ref><ref>PMID:19652551</ref><ref>PMID:19283071</ref>

	==About this Structure==		==About this Structure==
Line 14:		Line 16:

	==Reference==		==Reference==
	<ref group="xtra">PMID:016622405</ref><references group="xtra"/>		<ref group="xtra">PMID:016622405</ref><references group="xtra"/><references/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Perry, J J.]]		[[Category: Perry, J J.]]

OCA at 17:20, 20 October 2012

2012-10-20T17:20:10Z

← Older revision		Revision as of 17:20, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[2fc0]] is a 1 chain structure ~~of [[Helicase]]~~ with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FC0 OCA].		[[2fc0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FC0 OCA].

	==See Also==		==See Also==

OCA: Protected "2fc0" [edit=sysop:move=sysop]

2012-07-27T00:35:27Z

Protected "2fc0" [edit=sysop:move=sysop]

← Older revision	Revision as of 00:35, 27 July 2012
(No difference)

OCA at 00:35, 27 July 2012

2012-07-27T00:35:25Z

← Older revision		Revision as of 00:35, 27 July 2012
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	~~{{Seed}}~~
	[[Image:2fc0.png\|left\|200px]]		[[Image:2fc0.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2fc0", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2fc0\| PDB=2fc0 \| SCENE= }}		{{STRUCTURE_2fc0\| PDB=2fc0 \| SCENE= }}

	===WRN exonuclease, Mn dGMP complex===		===WRN exonuclease, Mn dGMP complex===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_16622405}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 16622405 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_16622405}}		{{ABSTRACT_PUBMED_16622405}}

	==About this Structure==		==About this Structure==
	~~2FC0~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FC0 OCA].		[[2fc0]] is a 1 chain structure of [[Helicase]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FC0 OCA].

			==See Also==
			*[[Helicase\|Helicase]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~16622405~~</ref><references group="xtra"/>		<ref group="xtra">PMID:016622405</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Perry, J J.]]		[[Category: Perry, J J.]]
Line 30:		Line 21:
	[[Category: Dnaq family]]		[[Category: Dnaq family]]
	[[Category: Recq]]		[[Category: Recq]]
			[[Category: Transferase]]
	[[Category: Werner syndrome]]		[[Category: Werner syndrome]]
	[[Category: Wrn]]		[[Category: Wrn]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 17:37:25 2009''~~