2fct - Revision history

OCA at 09:22, 14 February 2024

2024-02-14T09:22:42Z

← Older revision		Revision as of 09:22, 14 February 2024
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	<StructureSection load='2fct' size='340' side='right'caption='[[2fct]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='2fct' size='340' side='right'caption='[[2fct]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fct]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Psesy Psesy~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FCT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fct]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_syringae_pv._syringae Pseudomonas syringae pv. syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FCT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DSU:((2R,3S,4S,5S)-3,4-DIHYDROXY-5-(HYDROXYMETHYL)-5-((2R,3S,4S,5S,6R)-3,4,5-TRIHYDROXY-6-METHOXY-TETRAHYDRO-2H-PYRAN-2-YLOXY)-TETRAHYDROFURAN-2-YL)METHYL+NONANOATE'>DSU</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>~~</td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SyrB2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=321 PSESY])~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DSU:((2R,3S,4S,5S)-3,4-DIHYDROXY-5-(HYDROXYMETHYL)-5-((2R,3S,4S,5S,6R)-3,4,5-TRIHYDROXY-6-METHOXY-TETRAHYDRO-2H-PYRAN-2-YLOXY)-TETRAHYDROFURAN-2-YL)METHYL+NONANOATE'>DSU</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fct OCA], [https://pdbe.org/2fct PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fct RCSB], [https://www.ebi.ac.uk/pdbsum/2fct PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fct ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fct OCA], [https://pdbe.org/2fct PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fct RCSB], [https://www.ebi.ac.uk/pdbsum/2fct PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fct ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/SYRB2_PSESY SYRB2_PSESY] Involved in the biosynthesis of syringomycin E, a cyclic lipodepsinonapeptide toxin with phytotoxic activity (PubMed:16002467, PubMed:16528784, PubMed:19245217). Catalyzes the chlorination of L-Thr to 4-Cl-L-Thr when the amino acid is tethered via a thioester linkage to a covalently bound phosphopantetheine cofactor of the carrier protein SyrB1 (L-Thr-S-SyrB1), in a reaction that requires oxygen, chloride ions, ferrous iron and 2-oxoglutarate (PubMed:16002467, PubMed:16528784, PubMed:19245217). In vitro, can catalyze tandem chlorinations at the gamma-position of the threonyl-S-protein substrate (PubMed:16528784). Can also brominate L-Thr-S-SyrB1 in reactions performed with excess sodium bromide, but a preference for chloride versus bromide by a factor of 180 can be estimated by comparing the peak intensities (PubMed:16528784). In addition, in the presence of the nitrogenous anions NO2(-) or N3(-), SyrB2 can direct aliphatic nitration and azidation reactions by the same mechanism as the native halogenation reaction (PubMed:24463698).<ref>PMID:16002467</ref> <ref>PMID:16528784</ref> <ref>PMID:19245217</ref> <ref>PMID:24463698</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fct ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fct ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Non-haem Fe(II)/alpha-ketoglutarate (alphaKG)-dependent enzymes harness the reducing power of alphaKG to catalyse oxidative reactions, usually the hydroxylation of unactivated carbons, and are involved in processes such as natural product biosynthesis, the mammalian hypoxic response, and DNA repair. These enzymes couple the decarboxylation of alphaKG with the formation of a high-energy ferryl-oxo intermediate that acts as a hydrogen-abstracting species. All previously structurally characterized mononuclear iron enzymes contain a 2-His, 1-carboxylate motif that coordinates the iron. The two histidines and one carboxylate, known as the 'facial triad', form one triangular side of an octahedral iron coordination geometry. A subclass of mononuclear iron enzymes has been shown to catalyse halogenation reactions, rather than the more typical hydroxylation reaction. SyrB2, a member of this subclass, is a non-haem Fe(II)/alphaKG-dependent halogenase that catalyses the chlorination of threonine in syringomycin E biosynthesis. Here we report the structure of SyrB2 with both a chloride ion and alphaKG coordinated to the iron ion at 1.6 A resolution. This structure reveals a previously unknown coordination of iron, in which the carboxylate ligand of the facial triad is replaced by a chloride ion.

	Crystal structure of the non-haem iron halogenase SyrB2 in syringomycin biosynthesis.,Blasiak LC, Vaillancourt FH, Walsh CT, Drennan CL Nature. 2006 Mar 16;440(7082):368-71. PMID:16541079<ref>PMID:16541079</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2fct" style="background-color:#fffaf0;"></div>~~
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Psesy~~]]		[[Category: Pseudomonas syringae pv. syringae]]
	[[Category: Blasiak~~, L C~~]]		[[Category: Blasiak LC]]
	[[Category: Drennan~~, C L]]~~		[[Category: Drennan CL]]
	~~[[Category: Biosynthetic protein]]~~
	~~[[Category: Cupin]]~~
	~~[[Category: Halogenase]]~~
	~~[[Category: Mononuclear iron~~]]

OCA at 07:15, 24 February 2021

2021-02-24T07:15:02Z

← Older revision		Revision as of 07:15, 24 February 2021
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	==SyrB2 with Fe(II), chloride, and alpha-ketoglutarate==		==SyrB2 with Fe(II), chloride, and alpha-ketoglutarate==
	<StructureSection load='2fct' size='340' side='right' caption='[[2fct]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='2fct' size='340' side='right'caption='[[2fct]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fct]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Psesy Psesy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2FCT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fct]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Psesy Psesy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FCT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DSU:((2R,3S,4S,5S)-3,4-DIHYDROXY-5-(HYDROXYMETHYL)-5-((2R,3S,4S,5S,6R)-3,4,5-TRIHYDROXY-6-METHOXY-TETRAHYDRO-2H-PYRAN-2-YLOXY)-TETRAHYDROFURAN-2-YL)METHYL+NONANOATE'>DSU</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DSU:((2R,3S,4S,5S)-3,4-DIHYDROXY-5-(HYDROXYMETHYL)-5-((2R,3S,4S,5S,6R)-3,4,5-TRIHYDROXY-6-METHOXY-TETRAHYDRO-2H-PYRAN-2-YLOXY)-TETRAHYDROFURAN-2-YL)METHYL+NONANOATE'>DSU</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SyrB2 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=321 PSESY])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SyrB2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=321 PSESY])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2fct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fct OCA], [~~http~~://pdbe.org/2fct PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2fct RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2fct PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2fct ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fct OCA], [https://pdbe.org/2fct PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fct RCSB], [https://www.ebi.ac.uk/pdbsum/2fct PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fct ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Psesy]]		[[Category: Psesy]]
	[[Category: Blasiak, L C]]		[[Category: Blasiak, L C]]

OCA at 09:16, 14 June 2018

2018-06-14T09:16:39Z

← Older revision		Revision as of 09:16, 14 June 2018
Line 1:		Line 1:

	==SyrB2 with Fe(II), chloride, and alpha-ketoglutarate==		==SyrB2 with Fe(II), chloride, and alpha-ketoglutarate==
	<StructureSection load='2fct' size='340' side='right' caption='[[2fct]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='2fct' size='340' side='right' caption='[[2fct]], [[Resolution\|resolution]] 1.60Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DSU:((2R,3S,4S,5S)-3,4-DIHYDROXY-5-(HYDROXYMETHYL)-5-((2R,3S,4S,5S,6R)-3,4,5-TRIHYDROXY-6-METHOXY-TETRAHYDRO-2H-PYRAN-2-YLOXY)-TETRAHYDROFURAN-2-YL)METHYL+NONANOATE'>DSU</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DSU:((2R,3S,4S,5S)-3,4-DIHYDROXY-5-(HYDROXYMETHYL)-5-((2R,3S,4S,5S,6R)-3,4,5-TRIHYDROXY-6-METHOXY-TETRAHYDRO-2H-PYRAN-2-YLOXY)-TETRAHYDROFURAN-2-YL)METHYL+NONANOATE'>DSU</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SyrB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=321 PSESY])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SyrB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=321 PSESY])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fct OCA], [http://pdbe.org/2fct PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fct RCSB], [http://www.ebi.ac.uk/pdbsum/2fct PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fct OCA], [http://pdbe.org/2fct PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fct RCSB], [http://www.ebi.ac.uk/pdbsum/2fct PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2fct ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fc/2fct_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fc/2fct_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fct ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 14:50, 11 September 2015

2015-09-11T14:50:54Z

← Older revision		Revision as of 14:50, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2fct' size='340' side='right' caption='[[2fct]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='2fct' size='340' side='right' caption='[[2fct]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fct]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_syringae_pv._syringae Pseudomonas syringae pv. syringae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FCT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fct]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Psesy Psesy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FCT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DSU:((2R,3S,4S,5S)-3,4-DIHYDROXY-5-(HYDROXYMETHYL)-5-((2R,3S,4S,5S,6R)-3,4,5-TRIHYDROXY-6-METHOXY-TETRAHYDRO-2H-PYRAN-2-YLOXY)-TETRAHYDROFURAN-2-YL)METHYL+NONANOATE'>DSU</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DSU:((2R,3S,4S,5S)-3,4-DIHYDROXY-5-(HYDROXYMETHYL)-5-((2R,3S,4S,5S,6R)-3,4,5-TRIHYDROXY-6-METHOXY-TETRAHYDRO-2H-PYRAN-2-YLOXY)-TETRAHYDROFURAN-2-YL)METHYL+NONANOATE'>DSU</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SyrB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=321 ~~Pseudomonas syringae pv. syringae~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SyrB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=321 PSESY])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fct OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fct RCSB], [http://www.ebi.ac.uk/pdbsum/2fct PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fct OCA], [http://pdbe.org/2fct PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fct RCSB], [http://www.ebi.ac.uk/pdbsum/2fct PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2fct" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Pseudomonas syringae pv. syringae~~]]		[[Category: Psesy]]
	[[Category: Blasiak, L C]]		[[Category: Blasiak, L C]]
	[[Category: Drennan, C L]]		[[Category: Drennan, C L]]

OCA at 09:26, 16 January 2015

2015-01-16T09:26:07Z

← Older revision		Revision as of 09:26, 16 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fct]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_syringae_pv._syringae Pseudomonas syringae pv. syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FCT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fct]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_syringae_pv._syringae Pseudomonas syringae pv. syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FCT FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DSU:((2R,3S,4S,5S)-3,4-DIHYDROXY-5-(HYDROXYMETHYL)-5-((2R,3S,4S,5S,6R)-3,4,5-TRIHYDROXY-6-METHOXY-TETRAHYDRO-2H-PYRAN-2-YLOXY)-TETRAHYDROFURAN-2-YL)METHYL+NONANOATE'>DSU</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DSU:((2R,3S,4S,5S)-3,4-DIHYDROXY-5-(HYDROXYMETHYL)-5-((2R,3S,4S,5S,6R)-3,4,5-TRIHYDROXY-6-METHOXY-TETRAHYDRO-2H-PYRAN-2-YLOXY)-TETRAHYDROFURAN-2-YL)METHYL+NONANOATE'>DSU</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SyrB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=321 Pseudomonas syringae pv. syringae])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SyrB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=321 Pseudomonas syringae pv. syringae])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fct OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fct RCSB], [http://www.ebi.ac.uk/pdbsum/2fct PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fct OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fct RCSB], [http://www.ebi.ac.uk/pdbsum/2fct PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Pseudomonas syringae pv. syringae]]		[[Category: Pseudomonas syringae pv. syringae]]
	[[Category: Blasiak, L C.]]		[[Category: Blasiak, L C]]
	[[Category: Drennan, C L.]]		[[Category: Drennan, C L]]
	[[Category: Biosynthetic protein]]		[[Category: Biosynthetic protein]]
	[[Category: Cupin]]		[[Category: Cupin]]
	[[Category: Halogenase]]		[[Category: Halogenase]]
	[[Category: Mononuclear iron]]		[[Category: Mononuclear iron]]

OCA at 01:20, 30 September 2014

2014-09-30T01:20:47Z

← Older revision		Revision as of 01:20, 30 September 2014
Line 1:		Line 1:
	[[Image:~~2fct~~.~~png~~\|~~left~~\|~~200px~~]]		==SyrB2 with Fe(II), chloride, and alpha-ketoglutarate==
			<StructureSection load='2fct' size='340' side='right' caption='[[2fct]], [[Resolution\|resolution]] 1.60Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2fct]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_syringae_pv._syringae Pseudomonas syringae pv. syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FCT FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DSU:((2R,3S,4S,5S)-3,4-DIHYDROXY-5-(HYDROXYMETHYL)-5-((2R,3S,4S,5S,6R)-3,4,5-TRIHYDROXY-6-METHOXY-TETRAHYDRO-2H-PYRAN-2-YLOXY)-TETRAHYDROFURAN-2-YL)METHYL+NONANOATE'>DSU</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene><br>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SyrB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=321 Pseudomonas syringae pv. syringae])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fct OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fct RCSB], [http://www.ebi.ac.uk/pdbsum/2fct PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fc/2fct_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Non-haem Fe(II)/alpha-ketoglutarate (alphaKG)-dependent enzymes harness the reducing power of alphaKG to catalyse oxidative reactions, usually the hydroxylation of unactivated carbons, and are involved in processes such as natural product biosynthesis, the mammalian hypoxic response, and DNA repair. These enzymes couple the decarboxylation of alphaKG with the formation of a high-energy ferryl-oxo intermediate that acts as a hydrogen-abstracting species. All previously structurally characterized mononuclear iron enzymes contain a 2-His, 1-carboxylate motif that coordinates the iron. The two histidines and one carboxylate, known as the 'facial triad', form one triangular side of an octahedral iron coordination geometry. A subclass of mononuclear iron enzymes has been shown to catalyse halogenation reactions, rather than the more typical hydroxylation reaction. SyrB2, a member of this subclass, is a non-haem Fe(II)/alphaKG-dependent halogenase that catalyses the chlorination of threonine in syringomycin E biosynthesis. Here we report the structure of SyrB2 with both a chloride ion and alphaKG coordinated to the iron ion at 1.6 A resolution. This structure reveals a previously unknown coordination of iron, in which the carboxylate ligand of the facial triad is replaced by a chloride ion.

	~~{{STRUCTURE_2fct\| PDB=2fct \| SCENE= }}~~		Crystal structure of the non-haem iron halogenase SyrB2 in syringomycin biosynthesis.,Blasiak LC, Vaillancourt FH, Walsh CT, Drennan CL Nature. 2006 Mar 16;440(7082):368-71. PMID:16541079<ref>PMID:16541079</ref>

	~~===SyrB2 with Fe(II)~~, ~~chloride, and alpha-ketoglutarate===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_16541079}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2fct]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pseudomonas_syringae_pv~~.~~_syringae Pseudomonas syringae pv. syringae]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2FCT OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:016541079<~~/~~ref><ref group="xtra">PMID:018774824</ref~~><~~references group="xtra"~~/>
	[[Category: Pseudomonas syringae pv. syringae]]		[[Category: Pseudomonas syringae pv. syringae]]
	[[Category: Blasiak, L C.]]		[[Category: Blasiak, L C.]]

OCA: Protected "2fct" [edit=sysop:move=sysop]

2013-01-07T13:59:24Z

Protected "2fct" [edit=sysop:move=sysop]

← Older revision	Revision as of 13:59, 7 January 2013
(No difference)

OCA at 13:59, 7 January 2013

2013-01-07T13:59:21Z

← Older revision		Revision as of 13:59, 7 January 2013
Line 1:		Line 1:
	~~{{Seed}}~~
	[[Image:2fct.png\|left\|200px]]		[[Image:2fct.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2fct", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2fct\| PDB=2fct \| SCENE= }}		{{STRUCTURE_2fct\| PDB=2fct \| SCENE= }}

	===SyrB2 with Fe(II), chloride, and alpha-ketoglutarate===		===SyrB2 with Fe(II), chloride, and alpha-ketoglutarate===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_16541079}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 16541079 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_16541079}}		{{ABSTRACT_PUBMED_16541079}}

	==About this Structure==		==About this Structure==
	~~2FCT~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Pseudomonas_syringae_pv._syringae Pseudomonas syringae pv. syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCT OCA].		[[2fct]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_syringae_pv._syringae Pseudomonas syringae pv. syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCT OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~16541079~~</ref><references group="xtra"/>		<ref group="xtra">PMID:016541079</ref><ref group="xtra">PMID:018774824</ref><references group="xtra"/>
	[[Category: Pseudomonas syringae pv. syringae]]		[[Category: Pseudomonas syringae pv. syringae]]
	[[Category: Blasiak, L C.]]		[[Category: Blasiak, L C.]]
	[[Category: Drennan, C L.]]		[[Category: Drennan, C L.]]
			[[Category: Biosynthetic protein]]
	[[Category: Cupin]]		[[Category: Cupin]]
	[[Category: Halogenase]]		[[Category: Halogenase]]
	[[Category: Mononuclear iron]]		[[Category: Mononuclear iron]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 00:52:13 2009''~~

OCA at 22:52, 17 February 2009

2009-02-17T22:52:14Z

← Older revision		Revision as of 22:52, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
	2FCT is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Pseudomonas_syringae_pv._syringae Pseudomonas syringae pv. syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCT OCA].		2FCT is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_syringae_pv._syringae Pseudomonas syringae pv. syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCT OCA].

	==Reference==		==Reference==
	~~Crystal structure of the non-haem iron halogenase SyrB2 in syringomycin biosynthesis., Blasiak LC, Vaillancourt FH, Walsh CT, Drennan CL, Nature. 2006 Mar 16;440(7082):368-71.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/16541079 16541079]~~		<ref group="xtra">PMID:16541079</ref><references group="xtra"/>
	[[Category: Pseudomonas syringae pv. syringae]]		[[Category: Pseudomonas syringae pv. syringae]]
	~~[[Category: Single protein]]~~
	[[Category: Blasiak, L C.]]		[[Category: Blasiak, L C.]]
	[[Category: Drennan, C L.]]		[[Category: Drennan, C L.]]
Line 32:		Line 31:
	[[Category: Mononuclear iron]]		[[Category: Mononuclear iron]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 08~~:18:~~07 2008~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 00:52:13 2009''

OCA at 05:18, 29 July 2008

2008-07-29T05:18:08Z

← Older revision		Revision as of 05:18, 29 July 2008
Line 1:		Line 1:
	[[Image:2fct.~~gif~~\|left\|200px]]		{{Seed}}
			[[Image:2fct.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_2fct\| PDB=2fct \| SCENE= }}		{{STRUCTURE_2fct\| PDB=2fct \| SCENE= }}

	~~'''~~SyrB2 with Fe(II), chloride, and alpha-ketoglutarate~~'''~~		===SyrB2 with Fe(II), chloride, and alpha-ketoglutarate===


	~~==Overview==~~		<!--
	~~Non~~-~~haem Fe(II)/alpha~~-~~ketoglutarate (alphaKG)-dependent enzymes harness the reducing power of alphaKG to catalyse oxidative reactions, usually the hydroxylation of unactivated carbons~~, ~~and are involved in processes such as natural product biosynthesis~~, the ~~mammalian hypoxic response, and DNA repair. These enzymes couple~~ the ~~decarboxylation of alphaKG with the formation of a high-energy ferryl-oxo intermediate that acts~~ as ~~a hydrogen-abstracting species. All previously structurally characterized mononuclear iron enzymes contain a 2-His, 1-carboxylate motif that coordinates the iron~~. ~~The two histidines and one carboxylate, known as the 'facial triad', form one triangular side of an octahedral iron coordination geometry~~. ~~A subclass of mononuclear iron enzymes has been shown to catalyse halogenation reactions~~, ~~rather than~~ the ~~more typical hydroxylation reaction~~. ~~SyrB2, a member of this subclass, is a non~~-~~haem Fe(II)/alphaKG~~-dependent halogenase that catalyses the chlorination of threonine in syringomycin E biosynthesis. Here we report the structure of SyrB2 with both a chloride ion and alphaKG coordinated to the iron ion at 1.6 A resolution. This structure reveals a previously unknown coordination of iron, in which the carboxylate ligand of the facial triad is replaced by a chloride ion.		The line below this paragraph, {{ABSTRACT_PUBMED_16541079}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 16541079 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_16541079}}

	==About this Structure==		==About this Structure==
Line 27:		Line 31:
	[[Category: Halogenase]]		[[Category: Halogenase]]
	[[Category: Mononuclear iron]]		[[Category: Mononuclear iron]]
	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 03~~:44:34 2008''
			''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:18:07 2008''