2ff0 - Revision history

OCA at 18:56, 29 May 2024

2024-05-29T18:56:27Z

← Older revision		Revision as of 18:56, 29 May 2024
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	==Solution Structure of Steroidogenic Factor 1 DNA Binding Domain Bound to its Target Sequence in the Inhibin alpha-subunit Promoter==		==Solution Structure of Steroidogenic Factor 1 DNA Binding Domain Bound to its Target Sequence in the Inhibin alpha-subunit Promoter==
	<StructureSection load='2ff0' size='340' side='right'caption='[[2ff0~~]], [[NMR_Ensembles_of_Models \| 16 NMR models~~]]' scene=''>		<StructureSection load='2ff0' size='340' side='right'caption='[[2ff0]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ff0]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FF0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FF0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ff0]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FF0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FF0 FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~Nr5a1, Ftzf1 ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ff0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ff0 OCA], [https://pdbe.org/2ff0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ff0 RCSB], [https://www.ebi.ac.uk/pdbsum/2ff0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ff0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ff0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ff0 OCA], [https://pdbe.org/2ff0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ff0 RCSB], [https://www.ebi.ac.uk/pdbsum/2ff0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ff0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/STF1_MOUSE STF1_MOUSE]] Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity (By similarity). Transcription repressor of the Moloney leukemia virus long terminal repeat in undifferentiated murine embryonal carcinoma cells. Binds phosphatidylcholine and phospholipids with a phosphatidylinositol (PI) headgroup, in particular phosphatidyl(3,4)bisphosphate, phosphatidyl(3,5)bisphosphate and phosphatidyl(3,4,5)triphosphate. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.<ref>PMID:17210646</ref> <ref>PMID:18988706</ref>		[https://www.uniprot.org/uniprot/STF1_MOUSE STF1_MOUSE] Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity (By similarity). Transcription repressor of the Moloney leukemia virus long terminal repeat in undifferentiated murine embryonal carcinoma cells. Binds phosphatidylcholine and phospholipids with a phosphatidylinositol (PI) headgroup, in particular phosphatidyl(3,4)bisphosphate, phosphatidyl(3,5)bisphosphate and phosphatidyl(3,4,5)triphosphate. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.<ref>PMID:17210646</ref> <ref>PMID:18988706</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Lk3 transgenic mice~~]]		[[Category: Mus musculus]]
	[[Category: Little~~, T H~~]]		[[Category: Little TH]]
	[[Category: Matulis~~, C K~~]]		[[Category: Matulis CK]]
	[[Category: Mayo~~, K E~~]]		[[Category: Mayo KE]]
	[[Category: Radhakrishnan, I]]		[[Category: Radhakrishnan I]]
	[[Category: Ramachandran, A]]		[[Category: Ramachandran A]]
	[[Category: Weck, J]]		[[Category: Weck J]]
	[[Category: Zhang, Y]]		[[Category: Zhang Y]]
	[[Category: Zhang, Z]]		[[Category: Zhang Z]]
	~~[[Category: Hormone-growth factor-dna complex]]~~
	~~[[Category: Monomeric receptor-dna complex]]~~
	~~[[Category: Nuclear hormone receptor]]~~
	~~[[Category: Protein-dna complex~~]]

OCA at 07:16, 24 February 2021

2021-02-24T07:16:16Z

← Older revision		Revision as of 07:16, 24 February 2021
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	==Solution Structure of Steroidogenic Factor 1 DNA Binding Domain Bound to its Target Sequence in the Inhibin alpha-subunit Promoter==		==Solution Structure of Steroidogenic Factor 1 DNA Binding Domain Bound to its Target Sequence in the Inhibin alpha-subunit Promoter==
	<StructureSection load='2ff0' size='340' side='right' caption='[[2ff0]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>		<StructureSection load='2ff0' size='340' side='right'caption='[[2ff0]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ff0]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FF0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2FF0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ff0]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FF0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FF0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Nr5a1, Ftzf1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Nr5a1, Ftzf1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ff0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ff0 OCA], [~~http~~://pdbe.org/2ff0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ff0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ff0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ff0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ff0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ff0 OCA], [https://pdbe.org/2ff0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ff0 RCSB], [https://www.ebi.ac.uk/pdbsum/2ff0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ff0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/STF1_MOUSE STF1_MOUSE]] Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity (By similarity). Transcription repressor of the Moloney leukemia virus long terminal repeat in undifferentiated murine embryonal carcinoma cells. Binds phosphatidylcholine and phospholipids with a phosphatidylinositol (PI) headgroup, in particular phosphatidyl(3,4)bisphosphate, phosphatidyl(3,5)bisphosphate and phosphatidyl(3,4,5)triphosphate. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.<ref>PMID:17210646</ref> <ref>PMID:18988706</ref>		[[https://www.uniprot.org/uniprot/STF1_MOUSE STF1_MOUSE]] Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity (By similarity). Transcription repressor of the Moloney leukemia virus long terminal repeat in undifferentiated murine embryonal carcinoma cells. Binds phosphatidylcholine and phospholipids with a phosphatidylinositol (PI) headgroup, in particular phosphatidyl(3,4)bisphosphate, phosphatidyl(3,5)bisphosphate and phosphatidyl(3,4,5)triphosphate. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.<ref>PMID:17210646</ref> <ref>PMID:18988706</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Little, T H]]		[[Category: Little, T H]]

OCA at 09:14, 14 June 2018

2018-06-14T09:14:07Z

← Older revision		Revision as of 09:14, 14 June 2018
Line 1:		Line 1:

	==Solution Structure of Steroidogenic Factor 1 DNA Binding Domain Bound to its Target Sequence in the Inhibin alpha-subunit Promoter==		==Solution Structure of Steroidogenic Factor 1 DNA Binding Domain Bound to its Target Sequence in the Inhibin alpha-subunit Promoter==
	<StructureSection load='2ff0' size='340' side='right' caption='[[2ff0]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>		<StructureSection load='2ff0' size='340' side='right' caption='[[2ff0]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Nr5a1, Ftzf1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Nr5a1, Ftzf1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ff0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ff0 OCA], [http://pdbe.org/2ff0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ff0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ff0 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ff0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ff0 OCA], [http://pdbe.org/2ff0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ff0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ff0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ff0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ff/2ff0_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ff/2ff0_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 15:28, 8 February 2016

2016-02-08T15:28:06Z

← Older revision		Revision as of 15:28, 8 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ff0 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 12:57, 10 September 2015

2015-09-10T12:57:32Z

← Older revision		Revision as of 12:57, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2ff0' size='340' side='right' caption='[[2ff0]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>		<StructureSection load='2ff0' size='340' side='right' caption='[[2ff0]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ff0]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FF0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FF0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ff0]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FF0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FF0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Nr5a1, Ftzf1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Nr5a1, Ftzf1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ff0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ff0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ff0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ff0 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ff0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ff0 OCA], [http://pdbe.org/2ff0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ff0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ff0 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2ff0" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Mus musculus~~]]		[[Category: Lk3 transgenic mice]]
	[[Category: Little, T H]]		[[Category: Little, T H]]
	[[Category: Matulis, C K]]		[[Category: Matulis, C K]]

OCA at 00:20, 25 December 2014

2014-12-25T00:20:33Z

← Older revision		Revision as of 00:20, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ff0]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FF0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FF0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ff0]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FF0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FF0 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Nr5a1, Ftzf1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Nr5a1, Ftzf1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ff0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ff0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ff0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ff0 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ff0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ff0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ff0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ff0 PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/STF1_MOUSE STF1_MOUSE]] Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity (By similarity). Transcription repressor of the Moloney leukemia virus long terminal repeat in undifferentiated murine embryonal carcinoma cells. Binds phosphatidylcholine and phospholipids with a phosphatidylinositol (PI) headgroup, in particular phosphatidyl(3,4)bisphosphate, phosphatidyl(3,5)bisphosphate and phosphatidyl(3,4,5)triphosphate. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.<ref>PMID:17210646</ref> <ref>PMID:18988706</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Little, T H.]]		[[Category: Little, T H]]
	[[Category: Matulis, C K.]]		[[Category: Matulis, C K]]
	[[Category: Mayo, K E.]]		[[Category: Mayo, K E]]
	[[Category: Radhakrishnan, I.]]		[[Category: Radhakrishnan, I]]
	[[Category: Ramachandran, A.]]		[[Category: Ramachandran, A]]
	[[Category: Weck, J.]]		[[Category: Weck, J]]
	[[Category: Zhang, Y.]]		[[Category: Zhang, Y]]
	[[Category: Zhang, Z.]]		[[Category: Zhang, Z]]
	[[Category: Hormone-growth factor-dna complex]]		[[Category: Hormone-growth factor-dna complex]]
	[[Category: Monomeric receptor-dna complex]]		[[Category: Monomeric receptor-dna complex]]
	[[Category: Nuclear hormone receptor]]		[[Category: Nuclear hormone receptor]]
	[[Category: Protein-dna complex]]		[[Category: Protein-dna complex]]

OCA at 02:58, 30 September 2014

2014-09-30T02:58:11Z

← Older revision		Revision as of 02:58, 30 September 2014
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	[[Image:~~2ff0~~.~~png~~\|~~left~~\|~~200px~~]]		==Solution Structure of Steroidogenic Factor 1 DNA Binding Domain Bound to its Target Sequence in the Inhibin alpha-subunit Promoter==
			<StructureSection load='2ff0' size='340' side='right' caption='[[2ff0]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2ff0]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FF0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FF0 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Nr5a1, Ftzf1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ff0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ff0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ff0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ff0 PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ff/2ff0_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Steroidogenic factor 1 (SF1) is a member of the NR5A subfamily of nuclear hormone receptors and is considered a master regulator of reproduction because it regulates a number of genes encoding reproductive hormones and enzymes involved in steroid hormone biosynthesis. Like other NR5A members, SF1 harbors a highly conserved approximately 30-residue segment called the FTZ-F1 box C-terminal to the core DNA binding domain (DBD) common to all nuclear receptors and binds to 9-bp DNA sequences as a monomer. Here we describe the solution structure of the SF1 DBD in complex with an atypical sequence in the proximal promoter region of the inhibin-alpha gene that encodes a subunit of a reproductive hormone. SF1 forms a specific complex with the DNA through a bipartite motif binding to the major and minor grooves through the core DBD and the N-terminal segment of the FTZ-F1 box, respectively, in a manner previously described for two other monomeric receptors, nerve growth factor-induced-B and estrogen-related receptor 2. However, unlike these receptors, SF1 harbors a helix in the C-terminal segment of the FTZ-F1 box that interacts with both the core DBD and DNA and serves as an important determinant of stability of the complex. We propose that the FTZ-F1 helix along with the core DBD serves as a platform for interactions with coactivators and other DNA-bound factors in the vicinity.

	~~{{STRUCTURE_2ff0\| PDB=2ff0 \| SCENE= }}~~		Sequence-specific deoxyribonucleic acid (DNA) recognition by steroidogenic factor 1: a helix at the carboxy terminus of the DNA binding domain is necessary for complex stability.,Little TH, Zhang Y, Matulis CK, Weck J, Zhang Z, Ramachandran A, Mayo KE, Radhakrishnan I Mol Endocrinol. 2006 Apr;20(4):831-43. Epub 2005 Dec 8. PMID:16339274<ref>PMID:16339274</ref>

	~~===Solution Structure~~ of ~~Steroidogenic Factor 1 DNA Binding Domain Bound to its Target Sequence in~~ the ~~Inhibin alpha-subunit Promoter===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_16339274}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2ff0]] is a 3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2FF0 OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:016339274<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Little, T H.]]		[[Category: Little, T H.]]

OCA: Protected "2ff0" [edit=sysop:move=sysop]

2013-01-07T12:38:04Z

Protected "2ff0" [edit=sysop:move=sysop]

← Older revision	Revision as of 12:38, 7 January 2013
(No difference)

OCA at 12:38, 7 January 2013

2013-01-07T12:38:01Z

← Older revision		Revision as of 12:38, 7 January 2013
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	~~{{Seed}}~~
	[[Image:2ff0.png\|left\|200px]]		[[Image:2ff0.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2ff0", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2ff0\| PDB=2ff0 \| SCENE= }}		{{STRUCTURE_2ff0\| PDB=2ff0 \| SCENE= }}

	===Solution Structure of Steroidogenic Factor 1 DNA Binding Domain Bound to its Target Sequence in the Inhibin alpha-subunit Promoter===		===Solution Structure of Steroidogenic Factor 1 DNA Binding Domain Bound to its Target Sequence in the Inhibin alpha-subunit Promoter===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_16339274}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 16339274 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_16339274}}		{{ABSTRACT_PUBMED_16339274}}

	==About this Structure==		==About this Structure==
	~~2FF0~~ is a 3 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FF0 OCA].		[[2ff0]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FF0 OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~16339274~~</ref><references group="xtra"/>		<ref group="xtra">PMID:016339274</ref><references group="xtra"/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Little, T H.]]		[[Category: Little, T H.]]
Line 33:		Line 21:
	[[Category: Zhang, Y.]]		[[Category: Zhang, Y.]]
	[[Category: Zhang, Z.]]		[[Category: Zhang, Z.]]
			[[Category: Hormone-growth factor-dna complex]]
	[[Category: Monomeric receptor-dna complex]]		[[Category: Monomeric receptor-dna complex]]
	[[Category: Nuclear hormone receptor]]		[[Category: Nuclear hormone receptor]]
	[[Category: Protein-dna complex]]		[[Category: Protein-dna complex]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 00:01:20 2009''~~

OCA at 22:01, 16 February 2009

2009-02-16T22:01:21Z

← Older revision		Revision as of 22:01, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
	2FF0 is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FF0 OCA].		2FF0 is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FF0 OCA].

	==Reference==		==Reference==
	Sequence-specific deoxyribonucleic acid (DNA) recognition by steroidogenic factor 1: a helix at the carboxy terminus of the DNA binding domain is necessary for complex stability., Little TH, Zhang Y, Matulis CK, Weck J, Zhang Z, Ramachandran A, Mayo KE, Radhakrishnan I, Mol Endocrinol. 2006 Apr;20(4):831-43. Epub 2005 Dec 8. PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~16339274 16339274]~~		<ref group="xtra">PMID:16339274</ref><references group="xtra"/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	~~[[Category: Single protein]]~~
	[[Category: Little, T H.]]		[[Category: Little, T H.]]
	[[Category: Matulis, C K.]]		[[Category: Matulis, C K.]]
Line 38:		Line 37:
	[[Category: Protein-dna complex]]		[[Category: Protein-dna complex]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Jul 28 04~~:22:~~42 2008~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 00:01:20 2009''