2fgr - Revision history

OCA at 09:23, 14 February 2024

2024-02-14T09:23:28Z

← Older revision		Revision as of 09:23, 14 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fgr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Delftia_acidovorans Delftia acidovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FGR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FGR FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fgr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Delftia_acidovorans Delftia acidovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FGR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FGR FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height~~: ~~3em;~~'>~~[[1e54\|1e54]], [[2fgq\|2fgq]]~~</~~div~~></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fgr OCA], [https://pdbe.org/2fgr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fgr RCSB], [https://www.ebi.ac.uk/pdbsum/2fgr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fgr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fgr OCA], [https://pdbe.org/2fgr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fgr RCSB], [https://www.ebi.ac.uk/pdbsum/2fgr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fgr ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/OMP32_DELAC OMP32_DELAC]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fgr ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fgr ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The porin Omp32 is the major outer membrane protein of the bacterium Delftia acidovorans. The crystal structures of the strongly anion-selective porin alone and in complex with the substrate malate were solved at 1.5 and 1.45 A resolution, respectively, and revealed a malate-binding motif adjacent to the channel constriction zone. Binding is mediated by interaction with a cluster of two arginine residues and two threonines. This binding site is specific for Omp32 and reflects the physiological adaptation of the organism to organic acids. Structural studies are combined with a 7-ns unbiased molecular dynamics simulation of the trimeric channel in a model membrane. Molecular dynamics trajectories show how malate ions are efficiently captured from the surrounding bulk solution by the electrostatic potential of the channel, translocated to the binding site region, and immobilized in the constriction zone. In accordance with these results, conductance measurements with Omp32 inserted in planar lipid membranes revealed binding of malate. The anion-selective channel Omp32 is the first reported example of a porin with a 16-stranded beta-barrel and proven substrate specificity. This finding suggests a new view on the correlation of porin structure with substrate binding in specific channels.

	High resolution crystal structures and molecular dynamics studies reveal substrate binding in the porin Omp32.,Zachariae U, Kluhspies T, De S, Engelhardt H, Zeth K J Biol Chem. 2006 Mar 17;281(11):7413-20. Epub 2006 Jan 23. PMID:16434398<ref>PMID:16434398</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2fgr" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Porin 3D structures\|Porin 3D structures]]		*[[Porin 3D structures\|Porin 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Delftia acidovorans]]		[[Category: Delftia acidovorans]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Engelhardt, H]]		[[Category: Engelhardt H]]
	[[Category: Zachariae, U]]		[[Category: Zachariae U]]
	[[Category: Zeth, K]]		[[Category: Zeth K]]
	~~[[Category: Membrane protein]]~~
	~~[[Category: Omp32 porin outer membrane protein~~]]

OCA at 16:04, 22 December 2021

2021-12-22T16:04:47Z

← Older revision		Revision as of 16:04, 22 December 2021
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	<StructureSection load='2fgr' size='340' side='right'caption='[[2fgr]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2fgr' size='340' side='right'caption='[[2fgr]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fgr]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Delftia_acidovorans Delftia acidovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FGR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2FGR FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fgr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Delftia_acidovorans Delftia acidovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FGR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FGR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e54\|1e54]], [[2fgq\|2fgq]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1e54\|1e54]], [[2fgq\|2fgq]]</div></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2fgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fgr OCA], [~~http~~://pdbe.org/2fgr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2fgr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2fgr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2fgr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fgr OCA], [https://pdbe.org/2fgr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fgr RCSB], [https://www.ebi.ac.uk/pdbsum/2fgr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fgr ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 10:50, 18 March 2020

2020-03-18T10:50:02Z

← Older revision		Revision as of 10:50, 18 March 2020
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	==High resolution Xray structure of Omp32==		==High resolution Xray structure of Omp32==
	<StructureSection load='2fgr' size='340' side='right' caption='[[2fgr]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2fgr' size='340' side='right'caption='[[2fgr]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fgr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Delftia_acidovorans Delftia acidovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FGR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FGR FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fgr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Delftia_acidovorans Delftia acidovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FGR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FGR FirstGlance]. <br>
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	==See Also==		==See Also==
	*[[Porin\|Porin]]		*[[Porin 3D structures\|Porin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Delftia acidovorans]]		[[Category: Delftia acidovorans]]
			[[Category: Large Structures]]
	[[Category: Engelhardt, H]]		[[Category: Engelhardt, H]]
	[[Category: Zachariae, U]]		[[Category: Zachariae, U]]

OCA at 09:12, 14 June 2018

2018-06-14T09:12:12Z

← Older revision		Revision as of 09:12, 14 June 2018
Line 1:		Line 1:

	==High resolution Xray structure of Omp32==		==High resolution Xray structure of Omp32==
	<StructureSection load='2fgr' size='340' side='right' caption='[[2fgr]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2fgr' size='340' side='right' caption='[[2fgr]], [[Resolution\|resolution]] 1.50Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e54\|1e54]], [[2fgq\|2fgq]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e54\|1e54]], [[2fgq\|2fgq]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fgr OCA], [http://pdbe.org/2fgr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fgr RCSB], [http://www.ebi.ac.uk/pdbsum/2fgr PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fgr OCA], [http://pdbe.org/2fgr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fgr RCSB], [http://www.ebi.ac.uk/pdbsum/2fgr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2fgr ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fg/2fgr_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fg/2fgr_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 03:16, 7 February 2016

2016-02-07T03:16:57Z

← Older revision		Revision as of 03:16, 7 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fgr ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 21:08, 9 September 2015

2015-09-09T21:08:57Z

← Older revision		Revision as of 21:08, 9 September 2015
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e54\|1e54]], [[2fgq\|2fgq]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e54\|1e54]], [[2fgq\|2fgq]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fgr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fgr RCSB], [http://www.ebi.ac.uk/pdbsum/2fgr PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fgr OCA], [http://pdbe.org/2fgr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fgr RCSB], [http://www.ebi.ac.uk/pdbsum/2fgr PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2fgr" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 07:25, 24 December 2014

2014-12-24T07:25:13Z

← Older revision		Revision as of 07:25, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fgr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Delftia_acidovorans Delftia acidovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FGR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FGR FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fgr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Delftia_acidovorans Delftia acidovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FGR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FGR FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e54\|1e54]], [[2fgq\|2fgq]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e54\|1e54]], [[2fgq\|2fgq]]</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fgr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fgr RCSB], [http://www.ebi.ac.uk/pdbsum/2fgr PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fgr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fgr RCSB], [http://www.ebi.ac.uk/pdbsum/2fgr PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Delftia acidovorans]]		[[Category: Delftia acidovorans]]
	[[Category: Engelhardt, H.]]		[[Category: Engelhardt, H]]
	[[Category: Zachariae, U.]]		[[Category: Zachariae, U]]
	[[Category: Zeth, K.]]		[[Category: Zeth, K]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]
	[[Category: Omp32 porin outer membrane protein]]		[[Category: Omp32 porin outer membrane protein]]

OCA at 02:34, 29 September 2014

2014-09-29T02:34:04Z

← Older revision		Revision as of 02:34, 29 September 2014
Line 1:		Line 1:
	[[Image:~~2fgr~~.~~png~~\|~~left~~\|~~200px~~]]		==High resolution Xray structure of Omp32==
			<StructureSection load='2fgr' size='340' side='right' caption='[[2fgr]], [[Resolution\|resolution]] 1.50Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2fgr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Delftia_acidovorans Delftia acidovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FGR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FGR FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1e54\|1e54]], [[2fgq\|2fgq]]</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fgr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fgr RCSB], [http://www.ebi.ac.uk/pdbsum/2fgr PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fg/2fgr_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The porin Omp32 is the major outer membrane protein of the bacterium Delftia acidovorans. The crystal structures of the strongly anion-selective porin alone and in complex with the substrate malate were solved at 1.5 and 1.45 A resolution, respectively, and revealed a malate-binding motif adjacent to the channel constriction zone. Binding is mediated by interaction with a cluster of two arginine residues and two threonines. This binding site is specific for Omp32 and reflects the physiological adaptation of the organism to organic acids. Structural studies are combined with a 7-ns unbiased molecular dynamics simulation of the trimeric channel in a model membrane. Molecular dynamics trajectories show how malate ions are efficiently captured from the surrounding bulk solution by the electrostatic potential of the channel, translocated to the binding site region, and immobilized in the constriction zone. In accordance with these results, conductance measurements with Omp32 inserted in planar lipid membranes revealed binding of malate. The anion-selective channel Omp32 is the first reported example of a porin with a 16-stranded beta-barrel and proven substrate specificity. This finding suggests a new view on the correlation of porin structure with substrate binding in specific channels.

	~~{{STRUCTURE_2fgr\| PDB=2fgr \| SCENE= }}~~		High resolution crystal structures and molecular dynamics studies reveal substrate binding in the porin Omp32.,Zachariae U, Kluhspies T, De S, Engelhardt H, Zeth K J Biol Chem. 2006 Mar 17;281(11):7413-20. Epub 2006 Jan 23. PMID:16434398<ref>PMID:16434398</ref>

	~~===High resolution Xray structure~~ of ~~Omp32===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_16434398}}~~

	~~==About this Structure==~~
	~~[[2fgr]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Delftia_acidovorans Delftia acidovorans]~~. ~~Full crystallographic information is available from [http://oca.weizmann.ac.il~~/~~oca-bin/ocashort?id=2FGR OCA].~~

	==See Also==		==See Also==
	*[[Porin\|Porin]]		*[[Porin\|Porin]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:016434398<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Delftia acidovorans]]		[[Category: Delftia acidovorans]]
	[[Category: Engelhardt, H.]]		[[Category: Engelhardt, H.]]

OCA at 05:26, 20 October 2012

2012-10-20T05:26:33Z

← Older revision		Revision as of 05:26, 20 October 2012
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	==About this Structure==		==About this Structure==
	[[2fgr]] is a 2 chain structure ~~of [[Porin]]~~ with sequence from [http://en.wikipedia.org/wiki/Delftia_acidovorans Delftia acidovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FGR OCA].		[[2fgr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Delftia_acidovorans Delftia acidovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FGR OCA].

	==See Also==		==See Also==

OCA at 10:02, 25 July 2012

2012-07-25T10:02:22Z

← Older revision		Revision as of 10:02, 25 July 2012
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	[[Image:2fgr.png\|left\|200px]]		[[Image:2fgr.png\|left\|200px]]

	~~<!--~~
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	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2fgr\| PDB=2fgr \| SCENE= }}		{{STRUCTURE_2fgr\| PDB=2fgr \| SCENE= }}

	===High resolution Xray structure of Omp32===		===High resolution Xray structure of Omp32===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_16434398}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 16434398 is the PubMed ID number.~~
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	{{ABSTRACT_PUBMED_16434398}}		{{ABSTRACT_PUBMED_16434398}}

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	==See Also==		==See Also==
	*[[Porin]]		*[[Porin\|Porin]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~16434398~~</ref><references group="xtra"/>		<ref group="xtra">PMID:016434398</ref><references group="xtra"/>
	[[Category: Delftia acidovorans]]		[[Category: Delftia acidovorans]]
	[[Category: Engelhardt, H.]]		[[Category: Engelhardt, H.]]
	[[Category: Zachariae, U.]]		[[Category: Zachariae, U.]]
	[[Category: Zeth, K.]]		[[Category: Zeth, K.]]
			[[Category: Membrane protein]]
	[[Category: Omp32 porin outer membrane protein]]		[[Category: Omp32 porin outer membrane protein]]