2flt - Revision history

OCA at 09:45, 25 December 2024

2024-12-25T09:45:06Z

← Older revision		Revision as of 09:45, 25 December 2024
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	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fl/2flt_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fl/2flt_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/~~initialview01~~.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>

OCA at 09:28, 30 August 2023

2023-08-30T09:28:51Z

← Older revision		Revision as of 09:28, 30 August 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2flt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Coryneform_bacterium Coryneform bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FLT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2flt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Coryneform_bacterium Coryneform bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FLT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2flt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2flt OCA], [https://pdbe.org/2flt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2flt RCSB], [https://www.ebi.ac.uk/pdbsum/2flt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2flt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2flt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2flt OCA], [https://pdbe.org/2flt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2flt RCSB], [https://www.ebi.ac.uk/pdbsum/2flt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2flt ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/Q6VPE5_9CORY Q6VPE5_9CORY]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	[[Category: Coryneform bacterium]]		[[Category: Coryneform bacterium]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Jong~~, R M.de]]~~		[[Category: De Jong RM]]
	~~[[Category: 3-chloroacrylic acid dehalogenase]]~~
	~~[[Category: 4-oxalocrotonate tautomerase]]~~
	~~[[Category: 4ot family]]~~
	~~[[Category: Covalent inhibitor]]~~
	~~[[Category: Hydratase]]~~
	~~[[Category: Hydrolase~~]]

OCA at 16:06, 22 December 2021

2021-12-22T16:06:22Z

← Older revision		Revision as of 16:06, 22 December 2021
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	<StructureSection load='2flt' size='340' side='right'caption='[[2flt]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2flt' size='340' side='right'caption='[[2flt]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2flt]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Coryneform_bacterium Coryneform bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2FLT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2flt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Coryneform_bacterium Coryneform bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FLT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2flt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2flt OCA], [~~http~~://pdbe.org/2flt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2flt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2flt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2flt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2flt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2flt OCA], [https://pdbe.org/2flt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2flt RCSB], [https://www.ebi.ac.uk/pdbsum/2flt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2flt ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 10:51, 18 March 2020

2020-03-18T10:51:42Z

← Older revision		Revision as of 10:51, 18 March 2020
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	==The X-ray structure of the cis-3-chloroacrylic acid dehalogenase cis-CaaD inactivated with (R)-Oxirane-2-carboxylate==		==The X-ray structure of the cis-3-chloroacrylic acid dehalogenase cis-CaaD inactivated with (R)-Oxirane-2-carboxylate==
	<StructureSection load='2flt' size='340' side='right' caption='[[2flt]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2flt' size='340' side='right'caption='[[2flt]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2flt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Coryneform_bacterium Coryneform bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FLT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2flt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Coryneform_bacterium Coryneform bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FLT FirstGlance]. <br>
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	==See Also==		==See Also==
	*[[Dehalogenase\|Dehalogenase]]		*[[Dehalogenase 3D structures\|Dehalogenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Coryneform bacterium]]		[[Category: Coryneform bacterium]]
			[[Category: Large Structures]]
	[[Category: Jong, R M.de]]		[[Category: Jong, R M.de]]
	[[Category: 3-chloroacrylic acid dehalogenase]]		[[Category: 3-chloroacrylic acid dehalogenase]]

OCA at 09:05, 14 June 2018

2018-06-14T09:05:10Z

← Older revision		Revision as of 09:05, 14 June 2018
Line 1:		Line 1:

	==The X-ray structure of the cis-3-chloroacrylic acid dehalogenase cis-CaaD inactivated with (R)-Oxirane-2-carboxylate==		==The X-ray structure of the cis-3-chloroacrylic acid dehalogenase cis-CaaD inactivated with (R)-Oxirane-2-carboxylate==
	<StructureSection load='2flt' size='340' side='right' caption='[[2flt]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2flt' size='340' side='right' caption='[[2flt]], [[Resolution\|resolution]] 2.10Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2flt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Coryneform_bacterium Coryneform bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FLT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2flt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Coryneform_bacterium Coryneform bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FLT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2flt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2flt OCA], [http://pdbe.org/2flt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2flt RCSB], [http://www.ebi.ac.uk/pdbsum/2flt PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2flt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2flt OCA], [http://pdbe.org/2flt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2flt RCSB], [http://www.ebi.ac.uk/pdbsum/2flt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2flt ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fl/2flt_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fl/2flt_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 05:10, 7 February 2016

2016-02-07T05:10:17Z

← Older revision		Revision as of 05:10, 7 February 2016
Line 14:		Line 14:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2flt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:10, 9 September 2015

2015-09-09T22:10:10Z

← Older revision		Revision as of 22:10, 9 September 2015
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	<table><tr><td colspan='2'>[[2flt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Coryneform_bacterium Coryneform bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FLT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2flt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Coryneform_bacterium Coryneform bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FLT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2flt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2flt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2flt RCSB], [http://www.ebi.ac.uk/pdbsum/2flt PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2flt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2flt OCA], [http://pdbe.org/2flt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2flt RCSB], [http://www.ebi.ac.uk/pdbsum/2flt PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2flt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 09:15, 16 January 2015

2015-01-16T09:15:27Z

← Older revision		Revision as of 09:15, 16 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2flt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Coryneform_bacterium Coryneform bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FLT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2flt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Coryneform_bacterium Coryneform bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FLT FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2flt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2flt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2flt RCSB], [http://www.ebi.ac.uk/pdbsum/2flt PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2flt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2flt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2flt RCSB], [http://www.ebi.ac.uk/pdbsum/2flt PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Coryneform bacterium]]		[[Category: Coryneform bacterium]]
	[[Category: Jong, R M.de.]]		[[Category: Jong, R M.de]]
	[[Category: 3-chloroacrylic acid dehalogenase]]		[[Category: 3-chloroacrylic acid dehalogenase]]
	[[Category: 4-oxalocrotonate tautomerase]]		[[Category: 4-oxalocrotonate tautomerase]]

OCA at 08:52, 30 September 2014

2014-09-30T08:52:13Z

← Older revision		Revision as of 08:52, 30 September 2014
Line 1:		Line 1:
	[[Image:~~2flt~~.~~png~~\|~~left~~\|~~200px~~]]		==The X-ray structure of the cis-3-chloroacrylic acid dehalogenase cis-CaaD inactivated with (R)-Oxirane-2-carboxylate==
			<StructureSection load='2flt' size='340' side='right' caption='[[2flt]], [[Resolution\|resolution]] 2.10Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2flt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Coryneform_bacterium Coryneform bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FLT FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene><br>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2flt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2flt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2flt RCSB], [http://www.ebi.ac.uk/pdbsum/2flt PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fl/2flt_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The bacterial degradation pathways for the nematocide 1,3-dichloropropene rely on hydrolytic dehalogenation reactions catalyzed by cis- and trans-3-chloroacrylic acid dehalogenases (cis-CaaD and CaaD, respectively). X-ray crystal structures of native cis-CaaD and cis-CaaD inactivated by (R)-oxirane-2-carboxylate were elucidated. They locate four known catalytic residues (Pro-1, Arg-70, Arg-73, and Glu-114) and two previously unknown, potential catalytic residues (His-28 and Tyr-103'). The Y103F and H28A mutants of these latter two residues displayed reductions in cis-CaaD activity confirming their importance in catalysis. The structure of the inactivated enzyme shows covalent modification of the Pro-1 nitrogen atom by (R)-2-hydroxypropanoate at the C3 position. The interactions in the complex implicate Arg-70 or a water molecule bound to Arg-70 as the proton donor for the epoxide ring-opening reaction and Arg-73 and His-28 as primary binding contacts for the carboxylate group. This proposed binding mode places the (R)-enantiomer, but not the (S)-enantiomer, in position to covalently modify Pro-1. The absence of His-28 (or an equivalent) in CaaD could account for the fact that CaaD is not inactivated by either enantiomer. The cis-CaaD structures support a mechanism in which Glu-114 and Tyr-103' activate a water molecule for addition to C3 of the substrate and His-28, Arg-70, and Arg-73 interact with the C1 carboxylate group to assist in substrate binding and polarization. Pro-1 provides a proton at C2. The involvement of His-28 and Tyr-103' distinguishes the cis-CaaD mechanism from the otherwise parallel CaaD mechanism. The two mechanisms probably evolved independently as the result of an early gene duplication of a common ancestor.

	~~{{STRUCTURE_2flt\| PDB=2flt \| SCENE= }}~~		Crystal structures of native and inactivated cis-3-chloroacrylic acid dehalogenase. Structural basis for substrate specificity and inactivation by (R)-oxirane-2-carboxylate.,de Jong RM, Bazzacco P, Poelarends GJ, Johnson WH Jr, Kim YJ, Burks EA, Serrano H, Thunnissen AM, Whitman CP, Dijkstra BW J Biol Chem. 2007 Jan 26;282(4):2440-9. Epub 2006 Nov 22. PMID:17121835<ref>PMID:17121835</ref>

	~~===The X-ray structure~~ of the ~~cis-3-chloroacrylic acid dehalogenase cis-CaaD inactivated with (R)-Oxirane-2-carboxylate===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>

	~~{{ABSTRACT_PUBMED_17121835}}~~		==See Also==
			*[[Dehalogenase\|Dehalogenase]]
	==~~About this Structure~~==		== References ==
	[[~~2flt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Coryneform_bacterium Coryneform bacterium~~]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLT OCA~~].		<references/>
			__TOC__
	==~~Reference~~==		</StructureSection>
	<~~ref group="xtra">PMID:017121835<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Coryneform bacterium]]		[[Category: Coryneform bacterium]]
	[[Category: Jong, R M.de.]]		[[Category: Jong, R M.de.]]

OCA: Protected "2flt" [edit=sysop:move=sysop]

2013-01-07T11:12:52Z

Protected "2flt" [edit=sysop:move=sysop]

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