2fpc - Revision history

OCA at 09:07, 6 November 2024

2024-11-06T09:07:12Z

← Older revision		Revision as of 09:07, 6 November 2024
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	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fp/2fpc_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fp/2fpc_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/~~initialview01~~.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fpc ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fpc ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The enzyme strictosidine synthase (STR1) from the Indian medicinal plant Rauvolfia serpentina is of primary importance for the biosynthetic pathway of the indole alkaloid ajmaline. Moreover, STR1 initiates all biosynthetic pathways leading to the entire monoterpenoid indole alkaloid family representing an enormous structural variety of approximately 2000 compounds in higher plants. The crystal structures of STR1 in complex with its natural substrates tryptamine and secologanin provide structural understanding of the observed substrate preference and identify residues lining the active site surface that contact the substrates. STR1 catalyzes a Pictet-Spengler-type reaction and represents a novel six-bladed beta-propeller fold in plant proteins. Structure-based sequence alignment revealed a common repetitive sequence motif (three hydrophobic residues are followed by a small residue and a hydrophilic residue), indicating a possible evolutionary relationship between STR1 and several sequence-unrelated six-bladed beta-propeller structures. Structural analysis and site-directed mutagenesis experiments demonstrate the essential role of Glu-309 in catalysis. The data will aid in deciphering the details of the reaction mechanism of STR1 as well as other members of this enzyme family.

			The structure of Rauvolfia serpentina strictosidine synthase is a novel six-bladed beta-propeller fold in plant proteins.,Ma X, Panjikar S, Koepke J, Loris E, Stockigt J Plant Cell. 2006 Apr;18(4):907-20. Epub 2006 Mar 10. PMID:16531499<ref>PMID:16531499</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 2fpc" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Strictosidine Synthase\|Strictosidine Synthase]]		*[[Strictosidine Synthase\|Strictosidine Synthase]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:26, 3 April 2024

2024-04-03T06:26:14Z

← Older revision		Revision as of 06:26, 3 April 2024
Line 3:		Line 3:
	<StructureSection load='2fpc' size='340' side='right'caption='[[2fpc]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2fpc' size='340' side='right'caption='[[2fpc]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fpc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ophioxylon_serpentinum Ophioxylon serpentinum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FPC FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fpc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rauvolfia_serpentina Rauvolfia serpentina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FPC FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SCG:METHYL+(2S~~,~~3R,4S)-2-(BETA-D-GLUCOPYRANOSYLOXY)-4-(2-OXOETHYL)-~~3~~-VINYL-3,4-DIHYDRO-2H-PYRAN-5-CARBOXYLATE'>SCG</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[2fp8\|2fp8]], [[2fp9\|2fp9]], [[2fpb\|2fpb]]</div></td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SCG:METHYL+(2S,3R,4S)-2-(BETA-D-GLUCOPYRANOSYLOXY)-4-(2-OXOETHYL)-3-VINYL-3,4-DIHYDRO-2H-PYRAN-5-CARBOXYLATE'>SCG</scene></td></tr>
	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/Strictosidine_synthase Strictosidine synthase]~~, ~~with EC number [https://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=~~4.3.3.2 4~~.3.3.2]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fpc OCA], [https://pdbe.org/2fpc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fpc RCSB], [https://www.ebi.ac.uk/pdbsum/2fpc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fpc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fpc OCA], [https://pdbe.org/2fpc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fpc RCSB], [https://www.ebi.ac.uk/pdbsum/2fpc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fpc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/STSY_RAUSE STSY_RAUSE]] Catalyzes the stereospecific condensation of tryptamine with secologanin to form strictosidine, the key intermediate of indole alkaloid biosynthesis.		[https://www.uniprot.org/uniprot/STSY_RAUSE STSY_RAUSE] Catalyzes the stereospecific condensation of tryptamine with secologanin to form strictosidine, the key intermediate of indole alkaloid biosynthesis.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 21:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fpc ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fpc ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The enzyme strictosidine synthase (STR1) from the Indian medicinal plant Rauvolfia serpentina is of primary importance for the biosynthetic pathway of the indole alkaloid ajmaline. Moreover, STR1 initiates all biosynthetic pathways leading to the entire monoterpenoid indole alkaloid family representing an enormous structural variety of approximately 2000 compounds in higher plants. The crystal structures of STR1 in complex with its natural substrates tryptamine and secologanin provide structural understanding of the observed substrate preference and identify residues lining the active site surface that contact the substrates. STR1 catalyzes a Pictet-Spengler-type reaction and represents a novel six-bladed beta-propeller fold in plant proteins. Structure-based sequence alignment revealed a common repetitive sequence motif (three hydrophobic residues are followed by a small residue and a hydrophilic residue), indicating a possible evolutionary relationship between STR1 and several sequence-unrelated six-bladed beta-propeller structures. Structural analysis and site-directed mutagenesis experiments demonstrate the essential role of Glu-309 in catalysis. The data will aid in deciphering the details of the reaction mechanism of STR1 as well as other members of this enzyme family.

	The structure of Rauvolfia serpentina strictosidine synthase is a novel six-bladed beta-propeller fold in plant proteins.,Ma X, Panjikar S, Koepke J, Loris E, Stockigt J Plant Cell. 2006 Apr;18(4):907-20. Epub 2006 Mar 10. PMID:16531499<ref>PMID:16531499</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2fpc" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Strictosidine Synthase\|Strictosidine Synthase]]		*[[Strictosidine Synthase\|Strictosidine Synthase]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Ophioxylon serpentinum]]~~		[[Category: Rauvolfia serpentina]]
	~~[[Category: Strictosidine synthase~~]]		[[Category: Panjikar S]]
	[[Category: Panjikar, S]]
	~~[[Category: Lyase]]~~
	~~[[Category: Six bladed beta propeller fold]]~~
	~~[[Category: Str1]]~~
	~~[[Category: Synthase~~]]

OCA at 09:55, 5 May 2021

2021-05-05T09:55:57Z

← Older revision		Revision as of 09:55, 5 May 2021
Line 3:		Line 3:
	<StructureSection load='2fpc' size='340' side='right'caption='[[2fpc]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2fpc' size='340' side='right'caption='[[2fpc]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fpc]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Devilpepper Devilpepper~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2FPC FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fpc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ophioxylon_serpentinum Ophioxylon serpentinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FPC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SCG:METHYL+(2S,3R,4S)-2-(BETA-D-GLUCOPYRANOSYLOXY)-4-(2-OXOETHYL)-3-VINYL-3,4-DIHYDRO-2H-PYRAN-5-CARBOXYLATE'>SCG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SCG:METHYL+(2S,3R,4S)-2-(BETA-D-GLUCOPYRANOSYLOXY)-4-(2-OXOETHYL)-3-VINYL-3,4-DIHYDRO-2H-PYRAN-5-CARBOXYLATE'>SCG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fp8\|2fp8]], [[2fp9\|2fp9]], [[2fpb\|2fpb]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2fp8\|2fp8]], [[2fp9\|2fp9]], [[2fpb\|2fpb]]</div></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Strictosidine_synthase Strictosidine synthase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.3.2 4.3.3.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Strictosidine_synthase Strictosidine synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.3.2 4.3.3.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2fpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fpc OCA], [~~http~~://pdbe.org/2fpc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2fpc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2fpc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2fpc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fpc OCA], [https://pdbe.org/2fpc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fpc RCSB], [https://www.ebi.ac.uk/pdbsum/2fpc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fpc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/STSY_RAUSE STSY_RAUSE]] Catalyzes the stereospecific condensation of tryptamine with secologanin to form strictosidine, the key intermediate of indole alkaloid biosynthesis.		[[https://www.uniprot.org/uniprot/STSY_RAUSE STSY_RAUSE]] Catalyzes the stereospecific condensation of tryptamine with secologanin to form strictosidine, the key intermediate of indole alkaloid biosynthesis.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 37:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Devilpepper]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
			[[Category: Ophioxylon serpentinum]]
	[[Category: Strictosidine synthase]]		[[Category: Strictosidine synthase]]
	[[Category: Panjikar, S]]		[[Category: Panjikar, S]]

OCA at 08:54, 24 July 2019

2019-07-24T08:54:26Z

← Older revision		Revision as of 08:54, 24 July 2019
Line 1:		Line 1:

	==Structure of Strictosidine Synthase, the Biosynthetic Entry to the Monoterpenoid Indole Alkaloid Family==		==Structure of Strictosidine Synthase, the Biosynthetic Entry to the Monoterpenoid Indole Alkaloid Family==
	<StructureSection load='2fpc' size='340' side='right' caption='[[2fpc]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2fpc' size='340' side='right'caption='[[2fpc]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fpc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Devilpepper Devilpepper]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FPC FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fpc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Devilpepper Devilpepper]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FPC FirstGlance]. <br>
Line 15:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fp/2fpc_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fp/2fpc_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 38:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Devilpepper]]		[[Category: Devilpepper]]
			[[Category: Large Structures]]
	[[Category: Strictosidine synthase]]		[[Category: Strictosidine synthase]]
	[[Category: Panjikar, S]]		[[Category: Panjikar, S]]

OCA at 07:42, 18 October 2017

2017-10-18T07:42:29Z

← Older revision		Revision as of 07:42, 18 October 2017
Line 1:		Line 1:

	==Structure of Strictosidine Synthase, the Biosynthetic Entry to the Monoterpenoid Indole Alkaloid Family==		==Structure of Strictosidine Synthase, the Biosynthetic Entry to the Monoterpenoid Indole Alkaloid Family==
	<StructureSection load='2fpc' size='340' side='right' caption='[[2fpc]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2fpc' size='340' side='right' caption='[[2fpc]], [[Resolution\|resolution]] 3.00Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fp8\|2fp8]], [[2fp9\|2fp9]], [[2fpb\|2fpb]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fp8\|2fp8]], [[2fp9\|2fp9]], [[2fpb\|2fpb]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Strictosidine_synthase Strictosidine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.3.2 4.3.3.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Strictosidine_synthase Strictosidine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.3.2 4.3.3.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fpc OCA], [http://pdbe.org/2fpc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fpc RCSB], [http://www.ebi.ac.uk/pdbsum/2fpc PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fpc OCA], [http://pdbe.org/2fpc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fpc RCSB], [http://www.ebi.ac.uk/pdbsum/2fpc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2fpc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 00:01, 8 February 2016

2016-02-08T00:01:10Z

← Older revision		Revision as of 00:01, 8 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fpc ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:03, 10 September 2015

2015-09-10T06:03:25Z

← Older revision		Revision as of 06:03, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2fpc' size='340' side='right' caption='[[2fpc]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2fpc' size='340' side='right' caption='[[2fpc]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fpc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Rauvolfia_serpentina Rauvolfia serpentina~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FPC FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fpc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Devilpepper Devilpepper]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FPC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SCG:METHYL+(2S,3R,4S)-2-(BETA-D-GLUCOPYRANOSYLOXY)-4-(2-OXOETHYL)-3-VINYL-3,4-DIHYDRO-2H-PYRAN-5-CARBOXYLATE'>SCG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SCG:METHYL+(2S,3R,4S)-2-(BETA-D-GLUCOPYRANOSYLOXY)-4-(2-OXOETHYL)-3-VINYL-3,4-DIHYDRO-2H-PYRAN-5-CARBOXYLATE'>SCG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fp8\|2fp8]], [[2fp9\|2fp9]], [[2fpb\|2fpb]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fp8\|2fp8]], [[2fp9\|2fp9]], [[2fpb\|2fpb]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Strictosidine_synthase Strictosidine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.3.2 4.3.3.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Strictosidine_synthase Strictosidine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.3.2 4.3.3.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fpc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fpc RCSB], [http://www.ebi.ac.uk/pdbsum/2fpc PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fpc OCA], [http://pdbe.org/2fpc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fpc RCSB], [http://www.ebi.ac.uk/pdbsum/2fpc PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2fpc" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Rauvolfia serpentina~~]]		[[Category: Devilpepper]]
	[[Category: Strictosidine synthase]]		[[Category: Strictosidine synthase]]
	[[Category: Panjikar, S]]		[[Category: Panjikar, S]]

OCA at 19:07, 24 December 2014

2014-12-24T19:07:36Z

← Older revision		Revision as of 19:07, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fpc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rauvolfia_serpentina Rauvolfia serpentina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FPC FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fpc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rauvolfia_serpentina Rauvolfia serpentina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FPC FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SCG:METHYL+(2S,3R,4S)-2-(BETA-D-GLUCOPYRANOSYLOXY)-4-(2-OXOETHYL)-3-VINYL-3,4-DIHYDRO-2H-PYRAN-5-CARBOXYLATE'>SCG</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SCG:METHYL+(2S,3R,4S)-2-(BETA-D-GLUCOPYRANOSYLOXY)-4-(2-OXOETHYL)-3-VINYL-3,4-DIHYDRO-2H-PYRAN-5-CARBOXYLATE'>SCG</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fp8\|2fp8]], [[2fp9\|2fp9]], [[2fpb\|2fpb]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fp8\|2fp8]], [[2fp9\|2fp9]], [[2fpb\|2fpb]]</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Strictosidine_synthase Strictosidine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.3.2 4.3.3.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Strictosidine_synthase Strictosidine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.3.2 4.3.3.2] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fpc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fpc RCSB], [http://www.ebi.ac.uk/pdbsum/2fpc PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fpc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fpc RCSB], [http://www.ebi.ac.uk/pdbsum/2fpc PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/STSY_RAUSE STSY_RAUSE]] Catalyzes the stereospecific condensation of tryptamine with secologanin to form strictosidine, the key intermediate of indole alkaloid biosynthesis.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 37:
	[[Category: Rauvolfia serpentina]]		[[Category: Rauvolfia serpentina]]
	[[Category: Strictosidine synthase]]		[[Category: Strictosidine synthase]]
	[[Category: Panjikar, S.]]		[[Category: Panjikar, S]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Six bladed beta propeller fold]]		[[Category: Six bladed beta propeller fold]]
	[[Category: Str1]]		[[Category: Str1]]
	[[Category: Synthase]]		[[Category: Synthase]]

OCA at 10:21, 30 September 2014

2014-09-30T10:21:31Z

← Older revision		Revision as of 10:21, 30 September 2014
Line 1:		Line 1:
	~~{{STRUCTURE_2fpc\| PDB=2fpc \| SCENE= }}~~		==Structure of Strictosidine Synthase, the Biosynthetic Entry to the Monoterpenoid Indole Alkaloid Family==
	===Structure of Strictosidine Synthase, the Biosynthetic Entry to the Monoterpenoid Indole Alkaloid Family===		<StructureSection load='2fpc' size='340' side='right' caption='[[2fpc]], [[Resolution\|resolution]] 3.00Å' scene=''>
	~~{{ABSTRACT_PUBMED_16531499}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[2fpc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rauvolfia_serpentina Rauvolfia serpentina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FPC FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SCG:METHYL+(2S,3R,4S)-2-(BETA-D-GLUCOPYRANOSYLOXY)-4-(2-OXOETHYL)-3-VINYL-3,4-DIHYDRO-2H-PYRAN-5-CARBOXYLATE'>SCG</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fp8\|2fp8]], [[2fp9\|2fp9]], [[2fpb\|2fpb]]</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Strictosidine_synthase Strictosidine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.3.2 4.3.3.2] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fpc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fpc RCSB], [http://www.ebi.ac.uk/pdbsum/2fpc PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fp/2fpc_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The enzyme strictosidine synthase (STR1) from the Indian medicinal plant Rauvolfia serpentina is of primary importance for the biosynthetic pathway of the indole alkaloid ajmaline. Moreover, STR1 initiates all biosynthetic pathways leading to the entire monoterpenoid indole alkaloid family representing an enormous structural variety of approximately 2000 compounds in higher plants. The crystal structures of STR1 in complex with its natural substrates tryptamine and secologanin provide structural understanding of the observed substrate preference and identify residues lining the active site surface that contact the substrates. STR1 catalyzes a Pictet-Spengler-type reaction and represents a novel six-bladed beta-propeller fold in plant proteins. Structure-based sequence alignment revealed a common repetitive sequence motif (three hydrophobic residues are followed by a small residue and a hydrophilic residue), indicating a possible evolutionary relationship between STR1 and several sequence-unrelated six-bladed beta-propeller structures. Structural analysis and site-directed mutagenesis experiments demonstrate the essential role of Glu-309 in catalysis. The data will aid in deciphering the details of the reaction mechanism of STR1 as well as other members of this enzyme family.

	~~==About this Structure==~~		The structure of Rauvolfia serpentina strictosidine synthase is a novel six-bladed beta-propeller fold in plant proteins.,Ma X, Panjikar S, Koepke J, Loris E, Stockigt J Plant Cell. 2006 Apr;18(4):907-20. Epub 2006 Mar 10. PMID:16531499<ref>PMID:16531499</ref>
	~~[[2fpc]]~~ is a ~~2 chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org/wiki/Rauvolfia_serpentina Rauvolfia serpentina]. Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.~~il/oca-bin~~/~~ocashort?id=2FPC OCA].~~
			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>

	==See Also==		==See Also==
	*[[Strictosidine Synthase\|Strictosidine Synthase]]		*[[Strictosidine Synthase\|Strictosidine Synthase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:016531499<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Rauvolfia serpentina]]		[[Category: Rauvolfia serpentina]]
	[[Category: Strictosidine synthase]]		[[Category: Strictosidine synthase]]

OCA at 10:45, 11 March 2013

2013-03-11T10:45:40Z

← Older revision		Revision as of 10:45, 11 March 2013
Line 1:		Line 1:
	~~[[Image:2fpc.png\|left\|200px]]~~

	{{STRUCTURE_2fpc\| PDB=2fpc \| SCENE= }}		{{STRUCTURE_2fpc\| PDB=2fpc \| SCENE= }}

	===Structure of Strictosidine Synthase, the Biosynthetic Entry to the Monoterpenoid Indole Alkaloid Family===		===Structure of Strictosidine Synthase, the Biosynthetic Entry to the Monoterpenoid Indole Alkaloid Family===

	{{ABSTRACT_PUBMED_16531499}}		{{ABSTRACT_PUBMED_16531499}}