2fps - Revision history

OCA at 09:31, 30 August 2023

2023-08-30T09:31:04Z

← Older revision		Revision as of 09:31, 30 August 2023
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	<StructureSection load='2fps' size='340' side='right'caption='[[2fps]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2fps' size='340' side='right'caption='[[2fps]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fps]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Eco57 Eco57~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FPS FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fps]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FPS FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em~~;~~'>[[2fpr\|2fpr]], [[2fpu\|2fpu]], [[2fpw\|2fpw]], [[2fpx\|2fpx]]</div>~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~HisB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=83334 ECO57])</td~~></tr>
	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Histidinol-phosphatase Histidinol-phosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.15 3.1.3.15]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fps OCA], [https://pdbe.org/2fps PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fps RCSB], [https://www.ebi.ac.uk/pdbsum/2fps PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fps ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fps OCA], [https://pdbe.org/2fps PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fps RCSB], [https://www.ebi.ac.uk/pdbsum/2fps PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fps ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/HIS7_ECO57 HIS7_ECO57]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Eco57]]~~		[[Category: Escherichia coli O157:H7]]
	~~[[Category~~: ~~Histidinol-phosphatase~~]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	~~[[Category: Structural genomic]]~~		[[Category: Cygler M]]
	[[Category: Cygler, M]]		[[Category: Matte A]]
	[[Category: Matte, A]]		[[Category: Rangarajan ES]]
	[[Category: Rangarajan~~, E S]]~~
	~~[[Category: Bifunctional enzyme]]~~
	~~[[Category: Bsgi]]~~
	~~[[Category: Hisb]]~~
	~~[[Category: Histidinol phosphate phosphatase]]~~
	~~[[Category: Hydrolase~~]]

OCA at 15:31, 3 March 2021

2021-03-03T15:31:28Z

← Older revision		Revision as of 15:31, 3 March 2021
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	==Crystal structure of the N-terminal domain of E.coli HisB- Apo Ca model.==		==Crystal structure of the N-terminal domain of E.coli HisB- Apo Ca model.==
	<StructureSection load='2fps' size='340' side='right' caption='[[2fps]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2fps' size='340' side='right'caption='[[2fps]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fps]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2FPS FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fps]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FPS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fpr\|2fpr]], [[2fpu\|2fpu]], [[2fpw\|2fpw]], [[2fpx\|2fpx]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2fpr\|2fpr]], [[2fpu\|2fpu]], [[2fpw\|2fpw]], [[2fpx\|2fpx]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HisB ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HisB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Histidinol-phosphatase Histidinol-phosphatase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.15 3.1.3.15] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Histidinol-phosphatase Histidinol-phosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.15 3.1.3.15] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2fps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fps OCA], [~~http~~://pdbe.org/2fps PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2fps RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2fps PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2fps ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fps OCA], [https://pdbe.org/2fps PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fps RCSB], [https://www.ebi.ac.uk/pdbsum/2fps PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fps ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	[[Category: Eco57]]		[[Category: Eco57]]
	[[Category: Histidinol-phosphatase]]		[[Category: Histidinol-phosphatase]]
			[[Category: Large Structures]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	[[Category: Cygler, M]]		[[Category: Cygler, M]]

OCA at 18:49, 24 January 2018

2018-01-24T18:49:34Z

← Older revision		Revision as of 18:49, 24 January 2018
Line 1:		Line 1:

	==Crystal structure of the N-terminal domain of E.coli HisB- Apo Ca model.==		==Crystal structure of the N-terminal domain of E.coli HisB- Apo Ca model.==
	<StructureSection load='2fps' size='340' side='right' caption='[[2fps]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2fps' size='340' side='right' caption='[[2fps]], [[Resolution\|resolution]] 2.20Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HisB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HisB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidinol-phosphatase Histidinol-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.15 3.1.3.15] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidinol-phosphatase Histidinol-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.15 3.1.3.15] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fps OCA], [http://pdbe.org/2fps PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fps RCSB], [http://www.ebi.ac.uk/pdbsum/2fps PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fps OCA], [http://pdbe.org/2fps PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fps RCSB], [http://www.ebi.ac.uk/pdbsum/2fps PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2fps ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fp/2fps_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fp/2fps_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fps ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 00:59, 12 September 2015

2015-09-12T00:59:58Z

← Older revision		Revision as of 00:59, 12 September 2015
Line 2:		Line 2:
	<StructureSection load='2fps' size='340' side='right' caption='[[2fps]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2fps' size='340' side='right' caption='[[2fps]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fps]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli_o157:h7 Escherichia coli o157:h7~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FPS FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fps]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco57 Eco57]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FPS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fpr\|2fpr]], [[2fpu\|2fpu]], [[2fpw\|2fpw]], [[2fpx\|2fpx]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fpr\|2fpr]], [[2fpu\|2fpu]], [[2fpw\|2fpw]], [[2fpx\|2fpx]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HisB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ~~Escherichia coli O157:H7~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HisB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidinol-phosphatase Histidinol-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.15 3.1.3.15] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidinol-phosphatase Histidinol-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.15 3.1.3.15] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fps OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fps RCSB], [http://www.ebi.ac.uk/pdbsum/2fps PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fps OCA], [http://pdbe.org/2fps PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fps RCSB], [http://www.ebi.ac.uk/pdbsum/2fps PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2fps" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Escherichia coli o157:h7~~]]		[[Category: Eco57]]
	[[Category: Histidinol-phosphatase]]		[[Category: Histidinol-phosphatase]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 09:23, 16 January 2015

2015-01-16T09:23:34Z

← Older revision		Revision as of 09:23, 16 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fps]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_o157:h7 Escherichia coli o157:h7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FPS FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fps]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_o157:h7 Escherichia coli o157:h7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FPS FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fpr\|2fpr]], [[2fpu\|2fpu]], [[2fpw\|2fpw]], [[2fpx\|2fpx]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fpr\|2fpr]], [[2fpu\|2fpu]], [[2fpw\|2fpw]], [[2fpx\|2fpx]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HisB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 Escherichia coli O157:H7])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HisB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 Escherichia coli O157:H7])</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidinol-phosphatase Histidinol-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.15 3.1.3.15] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidinol-phosphatase Histidinol-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.15 3.1.3.15] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fps OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fps RCSB], [http://www.ebi.ac.uk/pdbsum/2fps PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fps OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fps RCSB], [http://www.ebi.ac.uk/pdbsum/2fps PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	[[Category: Escherichia coli o157:h7]]		[[Category: Escherichia coli o157:h7]]
	[[Category: Histidinol-phosphatase]]		[[Category: Histidinol-phosphatase]]
	[[Category: ~~BSGI, Montreal-Kingston Bacterial~~ Structural ~~Genomics Initiative.~~]]		[[Category: Structural genomic]]
	[[Category: Cygler, M.]]		[[Category: Cygler, M]]
	[[Category: Matte, A.]]		[[Category: Matte, A]]
	[[Category: Rangarajan, E S]]		[[Category: Rangarajan, E S]]
	~~[[Category: Bacterial structure genomic]]~~		[[Category: Bifunctional enzyme]]
	[[Category: Bifunctional enzyme.]]
	[[Category: Bsgi]]		[[Category: Bsgi]]
	[[Category: Hisb]]		[[Category: Hisb]]
	[[Category: Histidinol phosphate phosphatase]]		[[Category: Histidinol phosphate phosphatase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	~~[[Category: Montreal-kingston bacterial structural genomics initiative]]~~
	~~[[Category: Structural genomic]]~~

OCA at 09:00, 30 September 2014

2014-09-30T09:00:10Z

← Older revision		Revision as of 09:00, 30 September 2014
Line 1:		Line 1:
	[[Image:~~2fps~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of the N-terminal domain of E.coli HisB- Apo Ca model.==
			<StructureSection load='2fps' size='340' side='right' caption='[[2fps]], [[Resolution\|resolution]] 2.20Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2fps]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_o157:h7 Escherichia coli o157:h7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FPS FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2fpr\|2fpr]], [[2fpu\|2fpu]], [[2fpw\|2fpw]], [[2fpx\|2fpx]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HisB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 Escherichia coli O157:H7])</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidinol-phosphatase Histidinol-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.15 3.1.3.15] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fps OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fps RCSB], [http://www.ebi.ac.uk/pdbsum/2fps PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fp/2fps_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			HisB from Escherichia coli is a bifunctional enzyme catalyzing the sixth and eighth steps of l-histidine biosynthesis. The N-terminal domain (HisB-N) possesses histidinol phosphate phosphatase activity, and its crystal structure shows a single domain with fold similarity to the haloacid dehalogenase (HAD) enzyme family. HisB-N forms dimers in the crystal and in solution. The structure shows the presence of a structural Zn(2+) ion stabilizing the conformation of an extended loop. Two metal binding sites were also identified in the active site. Their presence was further confirmed by isothermal titration calorimetry. HisB-N is active in the presence of Mg(2+), Mn(2+), Co(2+), or Zn(2+), but Ca(2+) has an inhibitory effect. We have determined structures of several intermediate states corresponding to snapshots along the reaction pathway, including that of the phosphoaspartate intermediate. A catalytic mechanism, different from that described for other HAD enzymes, is proposed requiring the presence of the second metal ion not found in the active sites of previously characterized HAD enzymes, to complete the second half-reaction. The proposed mechanism is reminiscent of two-Mg(2+) ion catalysis utilized by DNA and RNA polymerases and many nucleases. The structure also provides an explanation for the inhibitory effect of Ca(2+).

	~~{{STRUCTURE_2fps\| PDB=2fps \| SCENE= }}~~		Structural snapshots of Escherichia coli histidinol phosphate phosphatase along the reaction pathway.,Rangarajan ES, Proteau A, Wagner J, Hung MN, Matte A, Cygler M J Biol Chem. 2006 Dec 8;281(49):37930-41. Epub 2006 Sep 11. PMID:16966333<ref>PMID:16966333</ref>

	~~===Crystal structure~~ of the ~~N-terminal domain~~ of E.~~coli HisB- Apo Ca model.===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_16966333}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2fps]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Escherichia_coli_o157:h7 Escherichia coli o157:h7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPS OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:016966333<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Escherichia coli o157:h7]]		[[Category: Escherichia coli o157:h7]]
	[[Category: Histidinol-phosphatase]]		[[Category: Histidinol-phosphatase]]

OCA: Protected "2fps" [edit=sysop:move=sysop]

2013-01-07T13:57:16Z

Protected "2fps" [edit=sysop:move=sysop]

← Older revision	Revision as of 13:57, 7 January 2013
(No difference)

OCA at 13:57, 7 January 2013

2013-01-07T13:57:11Z

← Older revision		Revision as of 13:57, 7 January 2013
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	~~{{Seed}}~~
	[[Image:2fps.png\|left\|200px]]		[[Image:2fps.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2fps", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2fps\| PDB=2fps \| SCENE= }}		{{STRUCTURE_2fps\| PDB=2fps \| SCENE= }}

	===Crystal structure of the N-terminal domain of E.coli HisB- Apo Ca model.===		===Crystal structure of the N-terminal domain of E.coli HisB- Apo Ca model.===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_16966333}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 16966333 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_16966333}}		{{ABSTRACT_PUBMED_16966333}}

	==About this Structure==		==About this Structure==
	~~2FPS~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/~~Escherichia_coli~~ Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPS OCA].		[[2fps]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_o157:h7 Escherichia coli o157:h7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPS OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~16966333~~</ref><references group="xtra"/>		<ref group="xtra">PMID:016966333</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli o157:h7]]
	[[Category: Histidinol-phosphatase]]		[[Category: Histidinol-phosphatase]]
	[[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]]		[[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]]
Line 35:		Line 23:
	[[Category: Hisb]]		[[Category: Hisb]]
	[[Category: Histidinol phosphate phosphatase]]		[[Category: Histidinol phosphate phosphatase]]
			[[Category: Hydrolase]]
	[[Category: Montreal-kingston bacterial structural genomics initiative]]		[[Category: Montreal-kingston bacterial structural genomics initiative]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 08:49:26 2009''~~

OCA at 06:49, 18 February 2009

2009-02-18T06:49:28Z

← Older revision		Revision as of 06:49, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
	2FPS is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPS OCA].		2FPS is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FPS OCA].

	==Reference==		==Reference==
	Structural snapshots of Escherichia coli histidinol phosphate phosphatase along the reaction pathway., Rangarajan ES, Proteau A, Wagner J, Hung MN, Matte A, Cygler M, J Biol Chem. 2006 Dec 8;281(49):37930-41. Epub 2006 Sep 11. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/16966333 16966333]~~		<ref group="xtra">PMID:16966333</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Histidinol-phosphatase]]		[[Category: Histidinol-phosphatase]]
	~~[[Category: Single protein]]~~
	[[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]]		[[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]]
	[[Category: Cygler, M.]]		[[Category: Cygler, M.]]
Line 39:		Line 38:
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 15~~:01:~~13 2008~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 08:49:26 2009''

OCA at 12:01, 29 July 2008

2008-07-29T12:01:19Z

← Older revision		Revision as of 12:01, 29 July 2008
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	[[Image:2fps.~~jpg~~\|left\|200px]]		{{Seed}}
			[[Image:2fps.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_2fps\| PDB=2fps \| SCENE= }}		{{STRUCTURE_2fps\| PDB=2fps \| SCENE= }}

	~~'''~~Crystal structure of the N-terminal domain of E.coli HisB- Apo Ca model.~~'''~~		===Crystal structure of the N-terminal domain of E.coli HisB- Apo Ca model.===


	~~==Overview==~~		<!--
	~~HisB from Escherichia coli is a bifunctional enzyme catalyzing the sixth and eighth steps of l-histidine biosynthesis.~~ The ~~N-terminal domain (HisB-N) possesses histidinol phosphate phosphatase activity~~, ~~and its crystal structure shows a single domain with fold similarity~~ to the ~~haloacid dehalogenase~~ (~~HAD) enzyme family~~. ~~HisB-N forms dimers in the crystal and in solution~~. ~~The structure shows the presence of a structural Zn(2+~~) ~~ion stabilizing the conformation of an extended loop. Two metal binding sites were also identified in the active site. Their presence was further confirmed by isothermal titration calorimetry. HisB-N~~ is ~~active in~~ the ~~presence of Mg(2+), Mn(2+), Co(2+), or Zn(2+), but Ca(2+) has an inhibitory effect~~. We have determined structures of several intermediate states corresponding to snapshots along the reaction pathway, including that of the phosphoaspartate intermediate. A catalytic mechanism, different from that described for other HAD enzymes, is proposed requiring the presence of the second metal ion not found in the active sites of previously characterized HAD enzymes, to complete the second half-~~reaction. The proposed mechanism is reminiscent of two~~-~~Mg(2+) ion catalysis utilized by DNA and RNA polymerases and many nucleases. The structure also provides an explanation for the inhibitory effect of Ca(2+).~~		The line below this paragraph, {{ABSTRACT_PUBMED_16966333}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 16966333 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_16966333}}

	==About this Structure==		==About this Structure==
Line 34:		Line 38:
	[[Category: Montreal-kingston bacterial structural genomics initiative]]		[[Category: Montreal-kingston bacterial structural genomics initiative]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 04~~:10:46 2008''
			''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:01:13 2008''