2fss - Revision history

OCA at 18:57, 29 May 2024

2024-05-29T18:57:23Z

← Older revision		Revision as of 18:57, 29 May 2024
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	<StructureSection load='2fss' size='340' side='right'caption='[[2fss]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='2fss' size='340' side='right'caption='[[2fss]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fss]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_18810 Atcc 18810~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FSS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FSS FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fss]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_boidinii Candida boidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FSS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FSS FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em~~;~~'>[[2a1z\|2a1z]]</div>~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>Cbfdh ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~5477 ATCC 18810])</td~~><~~/tr>~~
	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fss OCA], [https://pdbe.org/2fss PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fss RCSB], [https://www.ebi.ac.uk/pdbsum/2fss PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fss ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fss OCA], [https://pdbe.org/2fss PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fss RCSB], [https://www.ebi.ac.uk/pdbsum/2fss PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fss ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/FDH_CANBO FDH_CANBO] Catalyzes the NAD(+)-dependent oxidation of formate to carbon dioxide. Formate oxidation is the final step in the methanol oxidation pathway in methylotrophic microorganisms. Has a role in the detoxification of exogenous formate in non-methylotrophic organisms.[HAMAP-Rule:MF_03210]<ref>PMID:1248477</ref> <ref>PMID:9226256</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Atcc 18810]]~~
	~~[[Category: Formate dehydrogenase]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Lamzin~~, V S~~]]		[[Category: Lamzin VS]]
	[[Category: Schirwitz, K]]		[[Category: Schirwitz K]]
	[[Category: Schmidt, A]]		[[Category: Schmidt A]]
	~~[[Category: Formate binding site]]~~
	~~[[Category: Nad binding site]]~~
	~~[[Category: Oxidoreductase]]~~
	~~[[Category: Protein homo dimer]]~~
	~~[[Category: Rossmann fold~~]]

OCA at 15:32, 3 March 2021

2021-03-03T15:32:57Z

← Older revision		Revision as of 15:32, 3 March 2021
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	==Candida boidinii formate dehydrogenase (FDH) K47E mutant==		==Candida boidinii formate dehydrogenase (FDH) K47E mutant==
	<StructureSection load='2fss' size='340' side='right' caption='[[2fss]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='2fss' size='340' side='right'caption='[[2fss]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fss]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_18810 Atcc 18810]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FSS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2FSS FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fss]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18810 Atcc 18810]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FSS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FSS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a1z\|2a1z]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2a1z\|2a1z]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cbfdh ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5477 ATCC 18810])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cbfdh ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5477 ATCC 18810])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2fss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fss OCA], [~~http~~://pdbe.org/2fss PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2fss RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2fss PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2fss ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fss OCA], [https://pdbe.org/2fss PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fss RCSB], [https://www.ebi.ac.uk/pdbsum/2fss PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fss ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fs/2fss_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fs/2fss_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</div>		</div>
	<div class="pdbe-citations 2fss" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2fss" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Formate dehydrogenase 3D structures\|Formate dehydrogenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Atcc 18810]]		[[Category: Atcc 18810]]
	[[Category: Formate dehydrogenase]]		[[Category: Formate dehydrogenase]]
			[[Category: Large Structures]]
	[[Category: Lamzin, V S]]		[[Category: Lamzin, V S]]
	[[Category: Schirwitz, K]]		[[Category: Schirwitz, K]]

OCA at 07:43, 18 October 2017

2017-10-18T07:43:50Z

← Older revision		Revision as of 07:43, 18 October 2017
Line 1:		Line 1:

	==Candida boidinii formate dehydrogenase (FDH) K47E mutant==		==Candida boidinii formate dehydrogenase (FDH) K47E mutant==
	<StructureSection load='2fss' size='340' side='right' caption='[[2fss]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='2fss' size='340' side='right' caption='[[2fss]], [[Resolution\|resolution]] 1.70Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cbfdh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5477 ATCC 18810])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cbfdh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5477 ATCC 18810])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fss OCA], [http://pdbe.org/2fss PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fss RCSB], [http://www.ebi.ac.uk/pdbsum/2fss PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fss OCA], [http://pdbe.org/2fss PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fss RCSB], [http://www.ebi.ac.uk/pdbsum/2fss PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2fss ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	</div>		</div>
	<div class="pdbe-citations 2fss" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2fss" style="background-color:#fffaf0;"></div>

	~~==See Also==~~
	*[[Formate dehydrogenase\|Formate dehydrogenase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 18:53, 8 February 2016

2016-02-08T18:53:06Z

← Older revision		Revision as of 18:53, 8 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fss ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 14:26, 10 September 2015

2015-09-10T14:26:04Z

← Older revision		Revision as of 14:26, 10 September 2015
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	<StructureSection load='2fss' size='340' side='right' caption='[[2fss]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='2fss' size='340' side='right' caption='[[2fss]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fss]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Candida_boidinii Candida boidinii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FSS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FSS FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fss]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18810 Atcc 18810]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FSS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FSS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a1z\|2a1z]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a1z\|2a1z]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cbfdh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5477 ~~Candida boidinii~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cbfdh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5477 ATCC 18810])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fss OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fss RCSB], [http://www.ebi.ac.uk/pdbsum/2fss PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fss OCA], [http://pdbe.org/2fss PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fss RCSB], [http://www.ebi.ac.uk/pdbsum/2fss PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2fss" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Candida boidinii~~]]		[[Category: Atcc 18810]]
	[[Category: Formate dehydrogenase]]		[[Category: Formate dehydrogenase]]
	[[Category: Lamzin, V S]]		[[Category: Lamzin, V S]]

OCA at 09:19, 16 January 2015

2015-01-16T09:19:33Z

OCA at 05:18, 29 September 2014

2014-09-29T05:18:14Z

← Older revision		Revision as of 05:18, 29 September 2014
Line 1:		Line 1:
	[[Image:~~2fss~~.~~png~~\|~~left~~\|~~200px~~]]		==Candida boidinii formate dehydrogenase (FDH) K47E mutant==
			<StructureSection load='2fss' size='340' side='right' caption='[[2fss]], [[Resolution\|resolution]] 1.70Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2fss]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_boidinii Candida boidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FSS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FSS FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a1z\|2a1z]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cbfdh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5477 Candida boidinii])</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fss OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fss RCSB], [http://www.ebi.ac.uk/pdbsum/2fss PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fs/2fss_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The understanding of the mechanism of enzymatic recovery of NADH is of biological and of considerable biotechnological interest, since the essential, but expensive, cofactor NADH is exhausted in asymmetric hydrogenation processes, but can be recovered by NAD(+)-dependent formate dehydrogenase (FDH). Most accepted for this purpose is the FDH from the yeast Candida boidinii (CbFDH), which, having relatively low thermostability and specific activity, has been targeted by enzyme engineering for several years. Optimization by mutagenesis studies was performed based on physiological studies and structure modeling. However, X-ray structural information has been required in order to clarify the enzymatic mechanism and to enhance the effectiveness and operational stability of enzymatic cofactor regenerators in biocatalytic enantiomer synthesis as well as to explain the observed biochemical differences between yeast and bacterial FDH. We designed two single-point mutants in CbFDH using an adapted surface engineering approach, and this allowed crystals suitable for high-resolution X-ray structural studies to be obtained. The mutations improved the crystallizability of the protein and also the catalytic properties and the stability of the enzyme. With these crystal structures, we explain the observed differences from both sources, and form the basis for further rational mutagenesis studies.

	~~{{STRUCTURE_2fss\| PDB=2fss \| SCENE= }}~~		High-resolution structures of formate dehydrogenase from Candida boidinii.,Schirwitz K, Schmidt A, Lamzin VS Protein Sci. 2007 Jun;16(6):1146-56. PMID:17525463<ref>PMID:17525463</ref>

	~~===Candida boidinii formate dehydrogenase (FDH) K47E mutant===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_17525463}}~~

	~~==About this Structure==~~
	~~[[2fss]] is~~ a ~~4 chain structure with sequence from [http://en.wikipedia~~.~~org/wiki/Candida_boidinii Candida boidinii]~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann.ac.il/oca-bin~~/~~ocashort?id=2FSS OCA].~~

	==See Also==		==See Also==
	*[[Formate dehydrogenase\|Formate dehydrogenase]]		*[[Formate dehydrogenase\|Formate dehydrogenase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:017525463<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Candida boidinii]]		[[Category: Candida boidinii]]
	[[Category: Formate dehydrogenase]]		[[Category: Formate dehydrogenase]]

OCA at 11:07, 20 October 2012

2012-10-20T11:07:53Z

← Older revision		Revision as of 11:07, 20 October 2012
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	==About this Structure==		==About this Structure==
	[[2fss]] is a 4 chain structure ~~of [[Formate dehydrogenase]]~~ with sequence from [http://en.wikipedia.org/wiki/Candida_boidinii Candida boidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FSS OCA].		[[2fss]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_boidinii Candida boidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FSS OCA].

	==See Also==		==See Also==

OCA: Protected "2fss" [edit=sysop:move=sysop]

2012-07-26T04:20:41Z

Protected "2fss" [edit=sysop:move=sysop]

← Older revision	Revision as of 04:20, 26 July 2012
(No difference)

OCA at 04:20, 26 July 2012

2012-07-26T04:20:39Z

← Older revision		Revision as of 04:20, 26 July 2012
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	~~{{Seed}}~~
	[[Image:2fss.png\|left\|200px]]		[[Image:2fss.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2fss", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
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	{{STRUCTURE_2fss\| PDB=2fss \| SCENE= }}		{{STRUCTURE_2fss\| PDB=2fss \| SCENE= }}

	===Candida boidinii formate dehydrogenase (FDH) K47E mutant===		===Candida boidinii formate dehydrogenase (FDH) K47E mutant===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_17525463}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 17525463 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_17525463}}		{{ABSTRACT_PUBMED_17525463}}

	==About this Structure==		==About this Structure==
	~~2FSS~~ is a 4 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Candida_boidinii Candida boidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FSS OCA].		[[2fss]] is a 4 chain structure of [[Formate dehydrogenase]] with sequence from [http://en.wikipedia.org/wiki/Candida_boidinii Candida boidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FSS OCA].

			==See Also==
			*[[Formate dehydrogenase\|Formate dehydrogenase]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~17525463~~</ref><references group="xtra"/>		<ref group="xtra">PMID:017525463</ref><references group="xtra"/>
	[[Category: Candida boidinii]]		[[Category: Candida boidinii]]
	[[Category: Formate dehydrogenase]]		[[Category: Formate dehydrogenase]]
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	[[Category: Protein homo dimer]]		[[Category: Protein homo dimer]]
	[[Category: Rossmann fold]]		[[Category: Rossmann fold]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 29 21:01:47 2009''~~

← Older revision		Revision as of 09:19, 16 January 2015
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2fss]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_boidinii Candida boidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FSS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FSS FirstGlance]. <br>		<table><tr><td colspan='2'>[[2fss]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_boidinii Candida boidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FSS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FSS FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a1z\|2a1z]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a1z\|2a1z]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cbfdh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5477 Candida boidinii])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cbfdh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5477 Candida boidinii])</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fss OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fss RCSB], [http://www.ebi.ac.uk/pdbsum/2fss PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fss OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2fss RCSB], [http://www.ebi.ac.uk/pdbsum/2fss PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	[[Category: Candida boidinii]]		[[Category: Candida boidinii]]
	[[Category: Formate dehydrogenase]]		[[Category: Formate dehydrogenase]]
	[[Category: Lamzin, V S.]]		[[Category: Lamzin, V S]]
	[[Category: Schirwitz, K.]]		[[Category: Schirwitz, K]]
	[[Category: Schmidt, A.]]		[[Category: Schmidt, A]]
	[[Category: Formate binding site]]		[[Category: Formate binding site]]
	[[Category: Nad binding site]]		[[Category: Nad binding site]]