2gex - Revision history

OCA at 18:59, 10 March 2021

2021-03-10T18:59:54Z

← Older revision		Revision as of 18:59, 10 March 2021
Line 1:		Line 1:

	==Crystal structure of SnoaL2 a putative hydroxylase from Streptomyces nogalater==		==Crystal structure of SnoaL2 a putative hydroxylase from Streptomyces nogalater==
	<StructureSection load='2gex' size='340' side='right' caption='[[2gex]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2gex' size='340' side='right'caption='[[2gex]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2gex]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/As_4.1442 As 4.1442]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GEX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2GEX FirstGlance]. <br>		<table><tr><td colspan='2'>[[2gex]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/As_4.1442 As 4.1442]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GEX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GEX FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2gey\|2gey]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2gey\|2gey]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">snoal2 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=38314 AS 4.1442])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">snoal2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=38314 AS 4.1442])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2gex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gex OCA], [~~http~~://pdbe.org/2gex PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2gex RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2gex PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2gex ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gex OCA], [https://pdbe.org/2gex PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gex RCSB], [https://www.ebi.ac.uk/pdbsum/2gex PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gex ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: As 4 1442]]		[[Category: As 4 1442]]
			[[Category: Large Structures]]
	[[Category: Beinker, P]]		[[Category: Beinker, P]]
	[[Category: Lohkamp, B]]		[[Category: Lohkamp, B]]

OCA at 07:10, 20 June 2018

2018-06-20T07:10:45Z

← Older revision		Revision as of 07:10, 20 June 2018
Line 1:		Line 1:

	==Crystal structure of SnoaL2 a putative hydroxylase from Streptomyces nogalater==		==Crystal structure of SnoaL2 a putative hydroxylase from Streptomyces nogalater==
	<StructureSection load='2gex' size='340' side='right' caption='[[2gex]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2gex' size='340' side='right' caption='[[2gex]], [[Resolution\|resolution]] 2.50Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2gey\|2gey]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2gey\|2gey]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">snoal2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=38314 AS 4.1442])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">snoal2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=38314 AS 4.1442])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gex OCA], [http://pdbe.org/2gex PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2gex RCSB], [http://www.ebi.ac.uk/pdbsum/2gex PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gex OCA], [http://pdbe.org/2gex PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2gex RCSB], [http://www.ebi.ac.uk/pdbsum/2gex PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2gex ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ge/2gex_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ge/2gex_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 17:01, 8 February 2016

2016-02-08T17:01:14Z

← Older revision		Revision as of 17:01, 8 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gex ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 13:37, 10 September 2015

2015-09-10T13:37:53Z

← Older revision		Revision as of 13:37, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2gex' size='340' side='right' caption='[[2gex]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2gex' size='340' side='right' caption='[[2gex]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2gex]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_nogalater Streptomyces nogalater~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GEX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GEX FirstGlance]. <br>		<table><tr><td colspan='2'>[[2gex]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1442 As 4.1442]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GEX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GEX FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2gey\|2gey]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2gey\|2gey]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">snoal2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=38314 ~~Streptomyces nogalater~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">snoal2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=38314 AS 4.1442])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gex OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2gex RCSB], [http://www.ebi.ac.uk/pdbsum/2gex PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gex OCA], [http://pdbe.org/2gex PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2gex RCSB], [http://www.ebi.ac.uk/pdbsum/2gex PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2gex" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Streptomyces nogalater~~]]		[[Category: As 4 1442]]
	[[Category: Beinker, P]]		[[Category: Beinker, P]]
	[[Category: Lohkamp, B]]		[[Category: Lohkamp, B]]

OCA at 08:25, 16 January 2015

2015-01-16T08:25:38Z

← Older revision		Revision as of 08:25, 16 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2gex]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_nogalater Streptomyces nogalater]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GEX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GEX FirstGlance]. <br>		<table><tr><td colspan='2'>[[2gex]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_nogalater Streptomyces nogalater]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GEX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GEX FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2gey\|2gey]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2gey\|2gey]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">snoal2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=38314 Streptomyces nogalater])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">snoal2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=38314 Streptomyces nogalater])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gex OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2gex RCSB], [http://www.ebi.ac.uk/pdbsum/2gex PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gex OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2gex RCSB], [http://www.ebi.ac.uk/pdbsum/2gex PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces nogalater]]		[[Category: Streptomyces nogalater]]
	[[Category: Beinker, P.]]		[[Category: Beinker, P]]
	[[Category: Lohkamp, B.]]		[[Category: Lohkamp, B]]
	[[Category: Schneider, G.]]		[[Category: Schneider, G]]
	[[Category: Alpha+beta barrel]]		[[Category: Alpha+beta barrel]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 08:05, 30 September 2014

2014-09-30T08:05:22Z

← Older revision		Revision as of 08:05, 30 September 2014
Line 1:		Line 1:
	[[Image:~~2gex~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of SnoaL2 a putative hydroxylase from Streptomyces nogalater==
			<StructureSection load='2gex' size='340' side='right' caption='[[2gex]], [[Resolution\|resolution]] 2.50Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2gex]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_nogalater Streptomyces nogalater]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GEX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GEX FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2gey\|2gey]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">snoal2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=38314 Streptomyces nogalater])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gex OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2gex RCSB], [http://www.ebi.ac.uk/pdbsum/2gex PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ge/2gex_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			SnoaL2 and AclR are homologous enzymes in the biosynthesis of the aromatic polyketides nogalamycin in Streptomyces nogalater and cinerubin in Streptomyces galilaeus, respectively. Evidence obtained from gene transfer experiments suggested that SnoaL2 catalyzes the hydroxylation of the C-1 carbon atom of the polyketide chain. Here we show that AclR is also involved in the production of 1-hydroxylated anthracyclines in vivo. The three-dimensional structure of SnoaL2 has been determined by multi-wavelength anomalous diffraction to 2.5A resolution, and that of AclR to 1.8A resolution using molecular replacement. Both enzymes are dimers in solution and in the crystal. The fold of the enzyme subunits consists of an alpha+beta barrel. The dimer interface is formed by packing of the beta-sheets from the two subunits against each other. In the interior of the alpha+beta barrel a hydrophobic cavity is formed that most likely binds the substrate and harbors the active site. The subunit fold and the architecture of the active site in SnoaL2 and AclR are similar to that of the polyketide cyclases SnoaL and AknH; however, they show completely different quaternary structures. A comparison of the active site pockets of the putative hydroxylases AclR and SnoaL2 with those of bona fide polyketide cyclases reveals distinct differences in amino acids lining the cavity that might be responsible for the switch in chemistry. The moderate degree of sequence similarity and the preservation of the three-dimensional fold of the polypeptide chain suggest that these enzymes are evolutionary related. Members of this enzyme family appear to have evolved from a common protein scaffold by divergent evolution to catalyze reactions chemically as diverse as aldol condensation and hydroxylation.

	~~{{STRUCTURE_2gex\| PDB=2gex \| SCENE= }}~~		Crystal structures of SnoaL2 and AclR: two putative hydroxylases in the biosynthesis of aromatic polyketide antibiotics.,Beinker P, Lohkamp B, Peltonen T, Niemi J, Mantsala P, Schneider G J Mol Biol. 2006 Jun 9;359(3):728-40. Epub 2006 Apr 19. PMID:16650858<ref>PMID:16650858</ref>

	~~===Crystal structure~~ of ~~SnoaL2 a putative hydroxylase from Streptomyces nogalater===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_16650858}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2gex]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptomyces_nogalater Streptomyces nogalater]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2GEX OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:016650858<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Streptomyces nogalater]]		[[Category: Streptomyces nogalater]]
	[[Category: Beinker, P.]]		[[Category: Beinker, P.]]

OCA: Protected "2gex" [edit=sysop:move=sysop]

2013-01-07T13:09:32Z

Protected "2gex" [edit=sysop:move=sysop]

← Older revision	Revision as of 13:09, 7 January 2013
(No difference)

OCA at 13:09, 7 January 2013

2013-01-07T13:09:30Z

← Older revision		Revision as of 13:09, 7 January 2013
Line 1:		Line 1:
	~~{{Seed}}~~
	[[Image:2gex.png\|left\|200px]]		[[Image:2gex.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2gex", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2gex\| PDB=2gex \| SCENE= }}		{{STRUCTURE_2gex\| PDB=2gex \| SCENE= }}

	===Crystal structure of SnoaL2 a putative hydroxylase from Streptomyces nogalater===		===Crystal structure of SnoaL2 a putative hydroxylase from Streptomyces nogalater===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_16650858}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 16650858 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_16650858}}		{{ABSTRACT_PUBMED_16650858}}

	==About this Structure==		==About this Structure==
	~~2GEX~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Streptomyces_nogalater Streptomyces nogalater]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GEX OCA].		[[2gex]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_nogalater Streptomyces nogalater]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GEX OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~16650858~~</ref><references group="xtra"/>		<ref group="xtra">PMID:016650858</ref><references group="xtra"/>
	[[Category: Streptomyces nogalater]]		[[Category: Streptomyces nogalater]]
	[[Category: Beinker, P.]]		[[Category: Beinker, P.]]
Line 29:		Line 17:
	[[Category: Schneider, G.]]		[[Category: Schneider, G.]]
	[[Category: Alpha+beta barrel]]		[[Category: Alpha+beta barrel]]
			[[Category: Oxidoreductase]]
	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 00:38:21 2009''~~

OCA at 22:38, 16 February 2009

2009-02-16T22:38:22Z

← Older revision		Revision as of 22:38, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
	2GEX is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Streptomyces_nogalater Streptomyces nogalater]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GEX OCA].		2GEX is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Streptomyces_nogalater Streptomyces nogalater]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GEX OCA].

	==Reference==		==Reference==
	Crystal structures of SnoaL2 and AclR: two putative hydroxylases in the biosynthesis of aromatic polyketide antibiotics., Beinker P, Lohkamp B, Peltonen T, Niemi J, Mantsala P, Schneider G, J Mol Biol. 2006 Jun 9;359(3):728-40. Epub 2006 Apr 19. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/16650858 16650858]~~		<ref group="xtra">PMID:16650858</ref><references group="xtra"/>
	~~[[Category: Single protein]]~~
	[[Category: Streptomyces nogalater]]		[[Category: Streptomyces nogalater]]
	[[Category: Beinker, P.]]		[[Category: Beinker, P.]]
Line 31:		Line 30:
	[[Category: Alpha+beta barrel]]		[[Category: Alpha+beta barrel]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Jul 28 05~~:06:~~10 2008~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 00:38:21 2009''

OCA at 02:06, 28 July 2008

2008-07-28T02:06:11Z

← Older revision		Revision as of 02:06, 28 July 2008
Line 1:		Line 1:
	[[Image:2gex.~~gif~~\|left\|200px]]		{{Seed}}
			[[Image:2gex.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_2gex\| PDB=2gex \| SCENE= }}		{{STRUCTURE_2gex\| PDB=2gex \| SCENE= }}

	~~'''~~Crystal structure of SnoaL2 a putative hydroxylase from Streptomyces nogalater~~'''~~		===Crystal structure of SnoaL2 a putative hydroxylase from Streptomyces nogalater===


	~~==Overview==~~		<!--
	SnoaL2 and AclR are homologous enzymes in the biosynthesis of the aromatic polyketides nogalamycin in Streptomyces nogalater and cinerubin in Streptomyces galilaeus, respectively. Evidence obtained from gene transfer experiments suggested that SnoaL2 catalyzes the hydroxylation of the C-~~1 carbon atom of the polyketide chain. Here we show that AclR is also involved in the production of 1~~-~~hydroxylated anthracyclines in vivo.~~ The ~~three-dimensional structure of SnoaL2 has been determined by multi-wavelength anomalous diffraction to 2.5A resolution~~, ~~and that of AclR~~ to ~~1.8A resolution using molecular replacement. Both enzymes are dimers in solution and in~~ the ~~crystal~~. ~~The fold of the enzyme subunits consists of an alpha+beta barrel~~. ~~The dimer interface~~ is ~~formed by packing of~~ the ~~beta~~-sheets from the two subunits against each other. In the interior of the alpha+beta barrel a hydrophobic cavity is formed that most likely binds the substrate and harbors the active site. The subunit fold and the architecture of the active site in SnoaL2 and AclR are similar to that of the polyketide cyclases SnoaL and AknH; however, they show completely different quaternary structures. A comparison of the active site pockets of the putative hydroxylases AclR and SnoaL2 with those of bona fide polyketide cyclases reveals distinct differences in amino acids lining the cavity that might be responsible for the switch in chemistry. The moderate degree of sequence similarity and the preservation of the three-dimensional fold of the polypeptide chain suggest that these enzymes are evolutionary related. Members of this enzyme family appear to have evolved from a common protein scaffold by divergent evolution to catalyze reactions chemically as diverse as aldol condensation and hydroxylation.		The line below this paragraph, {{ABSTRACT_PUBMED_16650858}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 16650858 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_16650858}}

	==About this Structure==		==About this Structure==
Line 26:		Line 30:
	[[Category: Schneider, G.]]		[[Category: Schneider, G.]]
	[[Category: Alpha+beta barrel]]		[[Category: Alpha+beta barrel]]
	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4~~ 05:01:32 2008''
			''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 05:06:10 2008''