2ghv - Revision history

OCA at 00:58, 21 November 2024

2024-11-21T00:58:32Z

← Older revision		Revision as of 00:58, 21 November 2024
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	<StructureSection load='2ghv' size='340' side='right'caption='[[2ghv]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2ghv' size='340' side='right'caption='[[2ghv]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ghv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Cvhsa Cvhsa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GHV FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ghv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome-related_coronavirus Severe acute respiratory syndrome-related coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GHV FirstGlance]. <br>
	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[2ghw\|2ghw]], [[2ajf\|2ajf]]</div></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">S ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax~~.~~cgi?mode=Info~~&~~srchmode=5&id=227859 CVHSA])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ghv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ghv OCA], [https://pdbe.org/2ghv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ghv RCSB], [https://www.ebi.ac.uk/pdbsum/2ghv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ghv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ghv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ghv OCA], [https://pdbe.org/2ghv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ghv RCSB], [https://www.ebi.ac.uk/pdbsum/2ghv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ghv ProSAT]</span></td></tr>
	</table>		</table>
	~~== Function ==~~
	[[https://www.uniprot.org/uniprot/SPIKE_CVHSA SPIKE_CVHSA]] S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. S2 is a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gh/2ghv_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gh/2ghv_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/~~initialview01~~.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
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	==See Also==		==See Also==
	*[[Sandbox 3001\|Sandbox 3001]]		*[[Sandbox 3001\|Sandbox 3001]]
	*[[Spike protein\|Spike protein]]		*[[Spike protein 3D structures\|Spike protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Cvhsa]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Farzan, M]]		[[Category: Severe acute respiratory syndrome-related coronavirus]]
	[[Category: Hwang~~, W C~~]]		[[Category: Farzan M]]
	[[Category: Jaroszewski, L]]		[[Category: Hwang WC]]
	[[Category: Liddington~~, R C~~]]		[[Category: Jaroszewski L]]
	[[Category: Lin, Y]]		[[Category: Liddington RC]]
	[[Category: Marasco~~, W A~~]]		[[Category: Lin Y]]
	[[Category: Santelli, E]]		[[Category: Marasco WA]]
	[[Category: Stec, B]]		[[Category: Santelli E]]
	[[Category: Sui, J]]		[[Category: Stec B]]
	~~[[Category: S protein]]~~		[[Category: Sui J]]
	~~[[Category: Sar]]~~
	~~[[Category: Viral protein~~]]

OCA at 19:04, 10 March 2021

2021-03-10T19:04:28Z

← Older revision		Revision as of 19:04, 10 March 2021
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	==Crystal structure of SARS spike protein receptor binding domain==		==Crystal structure of SARS spike protein receptor binding domain==
	<StructureSection load='2ghv' size='340' side='right' caption='[[2ghv]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2ghv' size='340' side='right'caption='[[2ghv]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ghv]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Cvhsa Cvhsa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2GHV FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ghv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cvhsa Cvhsa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GHV FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ghw\|2ghw]], [[2ajf\|2ajf]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ghw\|2ghw]], [[2ajf\|2ajf]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">S ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227859 CVHSA])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">S ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227859 CVHSA])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ghv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ghv OCA], [~~http~~://pdbe.org/2ghv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ghv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ghv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ghv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ghv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ghv OCA], [https://pdbe.org/2ghv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ghv RCSB], [https://www.ebi.ac.uk/pdbsum/2ghv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ghv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/SPIKE_CVHSA SPIKE_CVHSA]] S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. S2 is a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.		[[https://www.uniprot.org/uniprot/SPIKE_CVHSA SPIKE_CVHSA]] S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. S2 is a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</div>		</div>
	<div class="pdbe-citations 2ghv" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2ghv" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Sandbox 3001\|Sandbox 3001]]
			*[[Spike protein\|Spike protein]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Cvhsa]]		[[Category: Cvhsa]]
			[[Category: Large Structures]]
	[[Category: Farzan, M]]		[[Category: Farzan, M]]
	[[Category: Hwang, W C]]		[[Category: Hwang, W C]]

OCA at 07:09, 20 June 2018

2018-06-20T07:09:00Z

← Older revision		Revision as of 07:09, 20 June 2018
Line 1:		Line 1:

	==Crystal structure of SARS spike protein receptor binding domain==		==Crystal structure of SARS spike protein receptor binding domain==
	<StructureSection load='2ghv' size='340' side='right' caption='[[2ghv]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2ghv' size='340' side='right' caption='[[2ghv]], [[Resolution\|resolution]] 2.20Å' scene=''>
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	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ghw\|2ghw]], [[2ajf\|2ajf]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ghw\|2ghw]], [[2ajf\|2ajf]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">S ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227859 CVHSA])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">S ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227859 CVHSA])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ghv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ghv OCA], [http://pdbe.org/2ghv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ghv RCSB], [http://www.ebi.ac.uk/pdbsum/2ghv PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ghv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ghv OCA], [http://pdbe.org/2ghv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ghv RCSB], [http://www.ebi.ac.uk/pdbsum/2ghv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ghv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gh/2ghv_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gh/2ghv_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 02:50, 8 February 2016

2016-02-08T02:50:50Z

← Older revision		Revision as of 02:50, 8 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ghv ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:09, 10 September 2015

2015-09-10T07:09:46Z

← Older revision		Revision as of 07:09, 10 September 2015
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	<StructureSection load='2ghv' size='340' side='right' caption='[[2ghv]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='2ghv' size='340' side='right' caption='[[2ghv]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ghv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sars_coronavirus Sars coronavirus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GHV FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ghv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cvhsa Cvhsa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GHV FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ghw\|2ghw]], [[2ajf\|2ajf]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ghw\|2ghw]], [[2ajf\|2ajf]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">S ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227859 ~~SARS coronavirus~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">S ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227859 CVHSA])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ghv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ghv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ghv RCSB], [http://www.ebi.ac.uk/pdbsum/2ghv PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ghv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ghv OCA], [http://pdbe.org/2ghv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ghv RCSB], [http://www.ebi.ac.uk/pdbsum/2ghv PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2ghv" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Sars coronavirus~~]]		[[Category: Cvhsa]]
	[[Category: Farzan, M]]		[[Category: Farzan, M]]
	[[Category: Hwang, W C]]		[[Category: Hwang, W C]]

OCA at 20:02, 24 December 2014

2014-12-24T20:02:14Z

← Older revision		Revision as of 20:02, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ghv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GHV FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ghv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GHV FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ghw\|2ghw]], [[2ajf\|2ajf]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ghw\|2ghw]], [[2ajf\|2ajf]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">S ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227859 SARS coronavirus])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">S ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227859 SARS coronavirus])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ghv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ghv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ghv RCSB], [http://www.ebi.ac.uk/pdbsum/2ghv PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ghv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ghv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ghv RCSB], [http://www.ebi.ac.uk/pdbsum/2ghv PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/SPIKE_CVHSA SPIKE_CVHSA]] S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. S2 is a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Sars coronavirus]]		[[Category: Sars coronavirus]]
	[[Category: Farzan, M.]]		[[Category: Farzan, M]]
	[[Category: Hwang, W C.]]		[[Category: Hwang, W C]]
	[[Category: Jaroszewski, L.]]		[[Category: Jaroszewski, L]]
	[[Category: Liddington, R C.]]		[[Category: Liddington, R C]]
	[[Category: Lin, Y.]]		[[Category: Lin, Y]]
	[[Category: Marasco, W A.]]		[[Category: Marasco, W A]]
	[[Category: Santelli, E.]]		[[Category: Santelli, E]]
	[[Category: Stec, B.]]		[[Category: Stec, B]]
	[[Category: Sui, J.]]		[[Category: Sui, J]]
	[[Category: S protein]]		[[Category: S protein]]
	[[Category: Sar]]		[[Category: Sar]]
	[[Category: Viral protein]]		[[Category: Viral protein]]

OCA at 10:24, 3 October 2014

2014-10-03T10:24:31Z

← Older revision		Revision as of 10:24, 3 October 2014
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	~~{{STRUCTURE_2ghv~~\| ~~PDB~~=2ghv \| ~~SCENE~~= }}		==Crystal structure of SARS spike protein receptor binding domain==
	===~~Crystal~~ structure of SARS spike protein receptor binding domain~~===~~		<StructureSection load='2ghv' size='340' side='right' caption='[[2ghv]], [[Resolution\|resolution]] 2.20Å' scene=''>
	~~{{ABSTRACT_PUBMED_16954221}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[2ghv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GHV FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ghw\|2ghw]], [[2ajf\|2ajf]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">S ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227859 SARS coronavirus])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ghv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ghv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ghv RCSB], [http://www.ebi.ac.uk/pdbsum/2ghv PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gh/2ghv_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Severe acute respiratory syndrome (SARS) is a newly emerged infectious disease that caused pandemic spread in 2003. The etiological agent of SARS is a novel coronavirus (SARS-CoV). The coronaviral surface spike protein S is a type I transmembrane glycoprotein that mediates initial host binding via the cell surface receptor angiotensin-converting enzyme 2 (ACE2), as well as the subsequent membrane fusion events required for cell entry. Here we report the crystal structure of the S1 receptor binding domain (RBD) in complex with a neutralizing antibody, 80R, at 2.3 A resolution, as well as the structure of the uncomplexed S1 RBD at 2.2 A resolution. We show that the 80R-binding epitope on the S1 RBD overlaps very closely with the ACE2-binding site, providing a rationale for the strong binding and broad neutralizing ability of the antibody. We provide a structural basis for the differential effects of certain mutations in the spike protein on 80R versus ACE2 binding, including escape mutants, which should facilitate the design of immunotherapeutics to treat a future SARS outbreak. We further show that the RBD of S1 forms dimers via an extensive interface that is disrupted in receptor- and antibody-bound crystal structures, and we propose a role for the dimer in virus stability and infectivity.

	~~==Function==~~		Structural basis of neutralization by a human anti-severe acute respiratory syndrome spike protein antibody, 80R.,Hwang WC, Lin Y, Santelli E, Sui J, Jaroszewski L, Stec B, Farzan M, Marasco WA, Liddington RC J Biol Chem. 2006 Nov 10;281(45):34610-6. Epub 2006 Sep 5. PMID:16954221<ref>PMID:16954221</ref>
	~~[[http://www.uniprot.org/uniprot/SPIKE_CVHSA SPIKE_CVHSA]] S1 attaches the virion to the cell membrane~~ by ~~interacting with~~ human ~~ACE2 and CLEC4M/DC~~-~~SIGNR~~, ~~initiating the infection~~. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. S2 is a class I viral fusion protein. Under the current model, ~~the protein has at least three conformational states: pre-fusion native state~~, ~~pre-hairpin intermediate state~~, ~~and post-fusion hairpin state~~. ~~During viral and target cell membrane fusion, the coiled coil regions~~ (~~heptad repeats~~) ~~assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C~~-~~terminal region of the ectodomain~~. ~~The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes~~.

	~~==About this Structure==~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~[[2ghv]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Sars_coronavirus Sars coronavirus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2GHV OCA].~~		</div>
			== References ==
	==~~Reference~~==		<references/>
	~~<ref group="xtra">PMID:016954221</ref>~~<references ~~group="xtra"~~/><~~references~~/>		__TOC__
			</StructureSection>
	[[Category: Sars coronavirus]]		[[Category: Sars coronavirus]]
	[[Category: Farzan, M.]]		[[Category: Farzan, M.]]

OCA: Protected "2ghv" [edit=sysop:move=sysop]

2014-04-23T08:16:50Z

Protected "2ghv" [edit=sysop:move=sysop]

← Older revision	Revision as of 08:16, 23 April 2014
(No difference)

OCA at 08:16, 23 April 2014

2014-04-23T08:16:47Z

← Older revision		Revision as of 08:16, 23 April 2014
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	~~{{Seed}}~~
	~~[[Image:2ghv.png\|left\|200px]]~~

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2ghv", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2ghv\| PDB=2ghv \| SCENE= }}		{{STRUCTURE_2ghv\| PDB=2ghv \| SCENE= }}

	===Crystal structure of SARS spike protein receptor binding domain===		===Crystal structure of SARS spike protein receptor binding domain===
			{{ABSTRACT_PUBMED_16954221}}

			==Function==
	~~<!--~~		[[http://www.uniprot.org/uniprot/SPIKE_CVHSA SPIKE_CVHSA]] S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. S2 is a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
	~~The line below this paragraph, {{ABSTRACT_PUBMED_16954221}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov)~~, ~~where 16954221~~ is the ~~PubMed ID number~~.
	-->
	~~{{ABSTRACT_PUBMED_16954221}}~~

	==About this Structure==		==About this Structure==
	~~2GHV~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/~~Human_sars_coronavirus Human sars~~ coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHV OCA].		[[2ghv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHV OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~16954221~~</ref><references group="xtra"/>		<ref group="xtra">PMID:016954221</ref><references group="xtra"/><references/>
	[[Category: ~~Human sars~~ coronavirus]]		[[Category: Sars coronavirus]]
	[[Category: Farzan, M.]]		[[Category: Farzan, M.]]
	[[Category: Hwang, W C.]]		[[Category: Hwang, W C.]]
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	[[Category: S protein]]		[[Category: S protein]]
	[[Category: Sar]]		[[Category: Sar]]
			[[Category: Viral protein]]
	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Mon Feb 16 18:16:03 2009''~~

OCA at 16:16, 16 February 2009

2009-02-16T16:16:04Z

← Older revision		Revision as of 16:16, 16 February 2009
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	==About this Structure==		==About this Structure==
	2GHV is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHV OCA].		2GHV is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Human_sars_coronavirus Human sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHV OCA].

	==Reference==		==Reference==
	Structural basis of neutralization by a human anti-severe acute respiratory syndrome spike protein antibody, 80R., Hwang WC, Lin Y, Santelli E, Sui J, Jaroszewski L, Stec B, Farzan M, Marasco WA, Liddington RC, J Biol Chem. 2006 Nov 10;281(45):34610-6. Epub 2006 Sep 5. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/16954221 16954221]~~		<ref group="xtra">PMID:16954221</ref><references group="xtra"/>
	[[Category: Human sars coronavirus]]		[[Category: Human sars coronavirus]]
	~~[[Category: Single protein]]~~
	[[Category: Farzan, M.]]		[[Category: Farzan, M.]]
	[[Category: Hwang, W C.]]		[[Category: Hwang, W C.]]
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	[[Category: Sar]]		[[Category: Sar]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun Jul 27 21~~:56:~~55 2008~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 18:16:03 2009''