2ht2 - Revision history

OCA at 08:09, 30 October 2024

2024-10-30T08:09:22Z

← Older revision		Revision as of 08:09, 30 October 2024
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	<StructureSection load='2ht2' size='340' side='right'caption='[[2ht2]], [[Resolution\|resolution]] 3.32Å' scene=''>		<StructureSection load='2ht2' size='340' side='right'caption='[[2ht2]], [[Resolution\|resolution]] 3.32Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ht2]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HT2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HT2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ht2]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HT2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HT2 FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.32Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'>[[1ots\|1ots]], [[2ht3\|2ht3]], [[2ht4\|2ht4]], [[2htk\|2htk]], [[2htl\|2htl]], [[2hlf\|2hlf]]</div~~><~~/td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">clcA, eriC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ht2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ht2 OCA], [https://pdbe.org/2ht2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ht2 RCSB], [https://www.ebi.ac.uk/pdbsum/2ht2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ht2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ht2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ht2 OCA], [https://pdbe.org/2ht2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ht2 RCSB], [https://www.ebi.ac.uk/pdbsum/2ht2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ht2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/CLCA_ECOLI CLCA_ECOLI]] Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response.<ref>PMID:12384697</ref> <ref>PMID:14985752</ref> <ref>PMID:16341087</ref> <ref>PMID:16905147</ref> <ref>PMID:18678918</ref>		[https://www.uniprot.org/uniprot/CLCA_ECOLI CLCA_ECOLI] Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response.<ref>PMID:12384697</ref> <ref>PMID:14985752</ref> <ref>PMID:16341087</ref> <ref>PMID:16905147</ref> <ref>PMID:18678918</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ht/2ht2_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ht/2ht2_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/~~initialview01~~.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]		[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Accardi, A]]		[[Category: Accardi A]]
	[[Category: Dutzler, R]]		[[Category: Dutzler R]]
	[[Category: Lobet, S]]		[[Category: Lobet S]]
	[[Category: Miller, C]]		[[Category: Miller C]]
	[[Category: Williams, C]]		[[Category: Williams C]]
	~~[[Category: Clc family of channel and transporter]]~~
	~~[[Category: Fab complex]]~~
	~~[[Category: H+/cl- antiporter]]~~
	~~[[Category: Membrane protein~~]]

OCA at 07:21, 24 March 2021

2021-03-24T07:21:33Z

← Older revision		Revision as of 07:21, 24 March 2021
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	==Structure of the Escherichia coli ClC chloride channel Y445H mutant and Fab complex==		==Structure of the Escherichia coli ClC chloride channel Y445H mutant and Fab complex==
	<StructureSection load='2ht2' size='340' side='right' caption='[[2ht2]], [[Resolution\|resolution]] 3.32Å' scene=''>		<StructureSection load='2ht2' size='340' side='right'caption='[[2ht2]], [[Resolution\|resolution]] 3.32Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ht2]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [~~http~~://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HT2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2HT2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ht2]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HT2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HT2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ots\|1ots]], [[2ht3\|2ht3]], [[2ht4\|2ht4]], [[2htk\|2htk]], [[2htl\|2htl]], [[2hlf\|2hlf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ots\|1ots]], [[2ht3\|2ht3]], [[2ht4\|2ht4]], [[2htk\|2htk]], [[2htl\|2htl]], [[2hlf\|2hlf]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">clcA, eriC ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">clcA, eriC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ht2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ht2 OCA], [~~http~~://pdbe.org/2ht2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ht2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ht2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ht2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ht2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ht2 OCA], [https://pdbe.org/2ht2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ht2 RCSB], [https://www.ebi.ac.uk/pdbsum/2ht2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ht2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/CLCA_ECOLI CLCA_ECOLI]] Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response.<ref>PMID:12384697</ref> <ref>PMID:14985752</ref> <ref>PMID:16341087</ref> <ref>PMID:16905147</ref> <ref>PMID:18678918</ref>		[[https://www.uniprot.org/uniprot/CLCA_ECOLI CLCA_ECOLI]] Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response.<ref>PMID:12384697</ref> <ref>PMID:14985752</ref> <ref>PMID:16341087</ref> <ref>PMID:16905147</ref> <ref>PMID:18678918</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
			[[Category: Large Structures]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Accardi, A]]		[[Category: Accardi, A]]

OCA at 06:49, 27 June 2018

2018-06-27T06:49:03Z

← Older revision		Revision as of 06:49, 27 June 2018
Line 1:		Line 1:

	==Structure of the Escherichia coli ClC chloride channel Y445H mutant and Fab complex==		==Structure of the Escherichia coli ClC chloride channel Y445H mutant and Fab complex==
	<StructureSection load='2ht2' size='340' side='right' caption='[[2ht2]], [[Resolution\|resolution]] 3.32Å' scene=''>		<StructureSection load='2ht2' size='340' side='right' caption='[[2ht2]], [[Resolution\|resolution]] 3.32Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ots\|1ots]], [[2ht3\|2ht3]], [[2ht4\|2ht4]], [[2htk\|2htk]], [[2htl\|2htl]], [[2hlf\|2hlf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ots\|1ots]], [[2ht3\|2ht3]], [[2ht4\|2ht4]], [[2htk\|2htk]], [[2htl\|2htl]], [[2hlf\|2hlf]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">clcA, eriC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">clcA, eriC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ht2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ht2 OCA], [http://pdbe.org/2ht2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ht2 RCSB], [http://www.ebi.ac.uk/pdbsum/2ht2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ht2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ht2 OCA], [http://pdbe.org/2ht2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ht2 RCSB], [http://www.ebi.ac.uk/pdbsum/2ht2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ht2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ht/2ht2_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ht/2ht2_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ht2 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	==See Also==		==See Also==
	*[[Monoclonal ~~Antibody~~\|Monoclonal ~~Antibody~~]]		*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 01:51, 12 September 2015

2015-09-12T01:51:03Z

← Older revision		Revision as of 01:51, 12 September 2015
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	<StructureSection load='2ht2' size='340' side='right' caption='[[2ht2]], [[Resolution\|resolution]] 3.32Å' scene=''>		<StructureSection load='2ht2' size='340' side='right' caption='[[2ht2]], [[Resolution\|resolution]] 3.32Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ht2]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HT2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HT2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ht2]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HT2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HT2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ots\|1ots]], [[2ht3\|2ht3]], [[2ht4\|2ht4]], [[2htk\|2htk]], [[2htl\|2htl]], [[2hlf\|2hlf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ots\|1ots]], [[2ht3\|2ht3]], [[2ht4\|2ht4]], [[2htk\|2htk]], [[2htl\|2htl]], [[2hlf\|2hlf]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">clcA, eriC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">clcA, eriC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ht2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ht2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ht2 RCSB], [http://www.ebi.ac.uk/pdbsum/2ht2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ht2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ht2 OCA], [http://pdbe.org/2ht2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ht2 RCSB], [http://www.ebi.ac.uk/pdbsum/2ht2 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2ht2" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Escherichia~~ coli]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Accardi, A]]		[[Category: Accardi, A]]

OCA at 22:16, 25 December 2014

2014-12-25T22:16:10Z

← Older revision		Revision as of 22:16, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ht2]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HT2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HT2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ht2]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HT2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HT2 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ots\|1ots]], [[2ht3\|2ht3]], [[2ht4\|2ht4]], [[2htk\|2htk]], [[2htl\|2htl]], [[2hlf\|2hlf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ots\|1ots]], [[2ht3\|2ht3]], [[2ht4\|2ht4]], [[2htk\|2htk]], [[2htl\|2htl]], [[2hlf\|2hlf]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">clcA, eriC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">clcA, eriC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ht2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ht2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ht2 RCSB], [http://www.ebi.ac.uk/pdbsum/2ht2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ht2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ht2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ht2 RCSB], [http://www.ebi.ac.uk/pdbsum/2ht2 PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/CLCA_ECOLI CLCA_ECOLI]] Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response.<ref>PMID:12384697</ref> <ref>PMID:14985752</ref> <ref>PMID:16341087</ref> <ref>PMID:16905147</ref> <ref>PMID:18678918</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 37:
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Accardi, A.]]		[[Category: Accardi, A]]
	[[Category: Dutzler, R.]]		[[Category: Dutzler, R]]
	[[Category: Lobet, S.]]		[[Category: Lobet, S]]
	[[Category: Miller, C.]]		[[Category: Miller, C]]
	[[Category: Williams, C.]]		[[Category: Williams, C]]
	[[Category: Clc family of channel and transporter]]		[[Category: Clc family of channel and transporter]]
	[[Category: Fab complex]]		[[Category: Fab complex]]
	[[Category: H+/cl- antiporter]]		[[Category: H+/cl- antiporter]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]

OCA at 05:55, 29 September 2014

2014-09-29T05:55:40Z

← Older revision		Revision as of 05:55, 29 September 2014
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	[[Image:~~2ht2~~.~~png~~\|~~left~~\|~~200px~~]]		==Structure of the Escherichia coli ClC chloride channel Y445H mutant and Fab complex==
			<StructureSection load='2ht2' size='340' side='right' caption='[[2ht2]], [[Resolution\|resolution]] 3.32Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2ht2]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HT2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HT2 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ots\|1ots]], [[2ht3\|2ht3]], [[2ht4\|2ht4]], [[2htk\|2htk]], [[2htl\|2htl]], [[2hlf\|2hlf]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">clcA, eriC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ht2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ht2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ht2 RCSB], [http://www.ebi.ac.uk/pdbsum/2ht2 PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ht/2ht2_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The Cl-/H+ exchange-transporter CLC-ec1 mediates stoichiometric transmembrane exchange of two Cl- ions for one proton. A conserved tyrosine residue, Y445, coordinates one of the bound Cl- ions visible in the structure of this protein and is located near the intersection of the Cl- and H+ pathways. Mutants of this tyrosine were scrutinized for effects on the coupled transport of Cl- and H+ determined electrophysiologically and on protein structure determined crystallographically. Despite the strong conservation of Y445 in the CLC family, substitution of F or W at this position preserves wild-type transport behavior. Substitution by A, E, or H, however, produces uncoupled proteins with robust Cl- transport but greatly impaired movement of H+. The obligatory 2 Cl-/1 H+ stoichiometry is thus lost in these mutants. The structures of all the mutants are essentially identical to wild-type, but apparent anion occupancy in the Cl- binding region correlates with functional H+ coupling. In particular, as determined by anomalous diffraction in crystals grown in Br-, an electrophysiologically competent Cl- analogue, the well-coupled transporters show strong Br- electron density at the "inner" and "central" Cl- binding sites. However, in the uncoupled mutants, Br- density is absent at the central site, while still present at the inner site. An additional mutant, Y445L, is intermediate in both functional and structural features. This mutant clearly exchanges H+ for Cl-, but at a reduced H+-to-Cl- ratio; likewise, both the central and inner sites are occupied by Br-, but the central site shows lower Br- density than in wild-type (or in Y445F,W). The correlation between proton coupling and central-site occupancy argues that halide binding to the central transport site somehow facilitates movement of H+, a synergism that is not readily understood in terms of alternating-site antiport schemes.

	~~{{STRUCTURE_2ht2\| PDB=2ht2 \| SCENE= }}~~		Synergism between halide binding and proton transport in a CLC-type exchanger.,Accardi A, Lobet S, Williams C, Miller C, Dutzler R J Mol Biol. 2006 Sep 29;362(4):691-9. Epub 2006 Aug 2. PMID:16949616<ref>PMID:16949616</ref>

	~~===Structure~~ of the ~~Escherichia coli ClC chloride channel Y445H mutant and Fab complex===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_16949616}}~~

	~~==About this Structure==~~
	~~[[2ht2]] is a 6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli] and [http://en~~.~~wikipedia.org~~/~~wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HT2 OCA].~~

	==See Also==		==See Also==
	*[[Monoclonal Antibody\|Monoclonal Antibody]]		*[[Monoclonal Antibody\|Monoclonal Antibody]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:016949616<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]

OCA at 22:17, 20 October 2012

2012-10-20T22:17:31Z

← Older revision		Revision as of 22:17, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[2ht2]] is a 6 chain structure ~~of [[Monoclonal Antibody]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HT2 OCA].		[[2ht2]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HT2 OCA].

	==See Also==		==See Also==

OCA: Protected "2ht2" [edit=sysop:move=sysop]

2012-07-27T15:50:15Z

Protected "2ht2" [edit=sysop:move=sysop]

← Older revision	Revision as of 15:50, 27 July 2012
(No difference)

OCA at 15:50, 27 July 2012

2012-07-27T15:50:14Z

← Older revision		Revision as of 15:50, 27 July 2012
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	~~{{Seed}}~~
	[[Image:2ht2.png\|left\|200px]]		[[Image:2ht2.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2ht2", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2ht2\| PDB=2ht2 \| SCENE= }}		{{STRUCTURE_2ht2\| PDB=2ht2 \| SCENE= }}

	===Structure of the Escherichia coli ClC chloride channel Y445H mutant and Fab complex===		===Structure of the Escherichia coli ClC chloride channel Y445H mutant and Fab complex===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_16949616}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 16949616 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_16949616}}		{{ABSTRACT_PUBMED_16949616}}

	==About this Structure==		==About this Structure==
	~~2HT2~~ is a 6 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HT2 OCA].		[[2ht2]] is a 6 chain structure of [[Monoclonal Antibody]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HT2 OCA].

			==See Also==
			*[[Monoclonal Antibody\|Monoclonal Antibody]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~16949616~~</ref><references group="xtra"/>		<ref group="xtra">PMID:016949616</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
Line 35:		Line 26:
	[[Category: H+/cl- antiporter]]		[[Category: H+/cl- antiporter]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 09:31:18 2009''~~

OCA at 07:31, 18 February 2009

2009-02-18T07:31:19Z

← Older revision		Revision as of 07:31, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
	2HT2 is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HT2 OCA].		2HT2 is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HT2 OCA].

	==Reference==		==Reference==
	~~Synergism between halide binding and proton transport in a CLC-type exchanger., Accardi A, Lobet S, Williams C, Miller C, Dutzler R, J Mol Biol. 2006 Sep 29;362(4):691-9. Epub 2006 Aug 2.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/16949616 16949616]~~		<ref group="xtra">PMID:16949616</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	~~[[Category: Single protein]]~~
	[[Category: Accardi, A.]]		[[Category: Accardi, A.]]
	[[Category: Dutzler, R.]]		[[Category: Dutzler, R.]]
Line 37:		Line 36:
	[[Category: Membrane protein]]		[[Category: Membrane protein]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 15~~:47:~~19 2008~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 09:31:18 2009''