2iak - Revision history

OCA at 09:02, 21 February 2024

2024-02-21T09:02:28Z

← Older revision		Revision as of 09:02, 21 February 2024
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	<StructureSection load='2iak' size='340' side='right'caption='[[2iak]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2iak' size='340' side='right'caption='[[2iak]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2iak]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IAK FirstGlance]. <br>		<table><tr><td colspan='2'>[[2iak]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IAK FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~Dst, Bpag1 ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iak OCA], [https://pdbe.org/2iak PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iak RCSB], [https://www.ebi.ac.uk/pdbsum/2iak PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iak ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/2iak TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iak OCA], [https://pdbe.org/2iak PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iak RCSB], [https://www.ebi.ac.uk/pdbsum/2iak PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iak ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/2iak TOPSAN]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/DYST_MOUSE DYST_MOUSE] Cytoskeletal linker protein. Acts as an integrator of intermediate filaments, actin and microtubule cytoskeleton networks. Required for anchoring either intermediate filaments to the actin cytoskeleton in neural and muscle cells or keratin-containing intermediate filaments to hemidesmosomes in epithelial cells. The proteins may self-aggregate to form filaments or a two-dimensional mesh. Regulates the organization and stability of the microtubule network of sensory neurons to allow axonal transport. Mediates docking of the dynein/dynactin motor complex to vesicle cargos for retrograde axonal transport through its interaction with TMEM108 and DCTN1.<ref>PMID:17287360</ref> Plays a structural role in the assembly of hemidesmosomes of epithelial cells; anchors keratin-containing intermediate filaments to the inner plaque of hemidesmosomes. Required for the regulation of keratinocyte polarity and motility; mediates integrin ITGB4 regulation of RAC1 activity. Required for bundling actin filaments around the nucleus.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2iak ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2iak ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Bullous pemphigoid antigen 1 (BPAG1) is a member of the plakin family of proteins. The plakins are multi-domain proteins that have been shown to interact with microtubules, actin filaments and intermediate filaments, as well as proteins found in cellular junctions. These interactions are mediated through different domains on the plakins. The interactions between plakins and components of specialized cell junctions such as desmosomes and hemidesmosomes are mediated through the so-called plakin domain, which is a common feature of the plakins. We report the crystal structure of a stable fragment from BPAG1, residues 226-448, defined by limited proteolysis of the whole plakin domain. The structure, determined by single-wavelength anomalous diffraction phasing from a selenomethionine-substituted crystal at 3.0 A resolution, reveals a tandem pair of triple helical bundles closely related to spectrin repeats. Based on this structure and analysis of sequence conservation, we propose that the architecture of plakin domains is defined by two pairs of spectrin repeats interrupted by a putative Src-Homology 3 (SH3) domain.

	Structural analysis of the plakin domain of bullous pemphigoid antigen1 (BPAG1) suggests that plakins are members of the spectrin superfamily.,Jefferson JJ, Ciatto C, Shapiro L, Liem RK J Mol Biol. 2007 Feb 9;366(1):244-57. Epub 2006 Nov 11. PMID:17161423<ref>PMID:17161423</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2iak" style="background-color:#fffaf0;"></div>~~
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Lk3 transgenic mice~~]]		[[Category: Mus musculus]]
	[[Category: Jefferson~~, J J]]~~		[[Category: Jefferson JJ]]
	~~[[Category: Cell adhesion]]~~
	~~[[Category: Spectrin repeat]]~~
	~~[[Category: Triple helical bundle~~]]

OCA at 11:12, 31 March 2021

2021-03-31T11:12:02Z

← Older revision		Revision as of 11:12, 31 March 2021
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	==Crystal Structure of a protease resistant fragment of the plakin domain of Bullous Pemphigoid Antigen1 (BPAG1)==		==Crystal Structure of a protease resistant fragment of the plakin domain of Bullous Pemphigoid Antigen1 (BPAG1)==
	<StructureSection load='2iak' size='340' side='right' caption='[[2iak]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2iak' size='340' side='right'caption='[[2iak]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2iak]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2IAK FirstGlance]. <br>		<table><tr><td colspan='2'>[[2iak]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IAK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dst, Bpag1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dst, Bpag1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2iak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iak OCA], [~~http~~://pdbe.org/2iak PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2iak RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2iak PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2iak ProSAT], [~~http~~://www.topsan.org/Proteins/NYSGXRC/2iak TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iak OCA], [https://pdbe.org/2iak PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iak RCSB], [https://www.ebi.ac.uk/pdbsum/2iak PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iak ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/2iak TOPSAN]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Jefferson, J J]]		[[Category: Jefferson, J J]]

OCA at 08:39, 4 July 2018

2018-07-04T08:39:17Z

← Older revision		Revision as of 08:39, 4 July 2018
Line 1:		Line 1:

	==Crystal Structure of a protease resistant fragment of the plakin domain of Bullous Pemphigoid Antigen1 (BPAG1)==		==Crystal Structure of a protease resistant fragment of the plakin domain of Bullous Pemphigoid Antigen1 (BPAG1)==
	<StructureSection load='2iak' size='340' side='right' caption='[[2iak]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2iak' size='340' side='right' caption='[[2iak]], [[Resolution\|resolution]] 3.00Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dst, Bpag1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dst, Bpag1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iak OCA], [http://pdbe.org/2iak PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2iak RCSB], [http://www.ebi.ac.uk/pdbsum/2iak PDBsum], [http://www.topsan.org/Proteins/NYSGXRC/2iak TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iak OCA], [http://pdbe.org/2iak PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2iak RCSB], [http://www.ebi.ac.uk/pdbsum/2iak PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2iak ProSAT], [http://www.topsan.org/Proteins/NYSGXRC/2iak TOPSAN]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ia/2iak_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ia/2iak_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 01:43, 8 February 2016

2016-02-08T01:43:58Z

← Older revision		Revision as of 01:43, 8 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2iak ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:42, 10 September 2015

2015-09-10T06:42:32Z

← Older revision		Revision as of 06:42, 10 September 2015
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	<StructureSection load='2iak' size='340' side='right' caption='[[2iak]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2iak' size='340' side='right' caption='[[2iak]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2iak]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IAK FirstGlance]. <br>		<table><tr><td colspan='2'>[[2iak]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IAK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dst, Bpag1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dst, Bpag1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iak OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2iak RCSB], [http://www.ebi.ac.uk/pdbsum/2iak PDBsum], [http://www.topsan.org/Proteins/NYSGXRC/2iak TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iak OCA], [http://pdbe.org/2iak PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2iak RCSB], [http://www.ebi.ac.uk/pdbsum/2iak PDBsum], [http://www.topsan.org/Proteins/NYSGXRC/2iak TOPSAN]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2iak" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Mus musculus~~]]		[[Category: Lk3 transgenic mice]]
	[[Category: Jefferson, J J]]		[[Category: Jefferson, J J]]
	[[Category: Cell adhesion]]		[[Category: Cell adhesion]]
	[[Category: Spectrin repeat]]		[[Category: Spectrin repeat]]
	[[Category: Triple helical bundle]]		[[Category: Triple helical bundle]]

OCA at 19:38, 24 December 2014

2014-12-24T19:38:42Z

← Older revision		Revision as of 19:38, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2iak]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IAK FirstGlance]. <br>		<table><tr><td colspan='2'>[[2iak]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IAK FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dst, Bpag1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dst, Bpag1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iak OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2iak RCSB], [http://www.ebi.ac.uk/pdbsum/2iak PDBsum], [http://www.topsan.org/Proteins/NYSGXRC/2iak TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iak OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2iak RCSB], [http://www.ebi.ac.uk/pdbsum/2iak PDBsum], [http://www.topsan.org/Proteins/NYSGXRC/2iak TOPSAN]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Jefferson, J J.]]		[[Category: Jefferson, J J]]
	[[Category: Cell adhesion]]		[[Category: Cell adhesion]]
	[[Category: Spectrin repeat]]		[[Category: Spectrin repeat]]
	[[Category: Triple helical bundle]]		[[Category: Triple helical bundle]]

OCA at 10:57, 30 September 2014

2014-09-30T10:57:55Z

← Older revision		Revision as of 10:57, 30 September 2014
Line 1:		Line 1:
	[[Image:~~2iak~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal Structure of a protease resistant fragment of the plakin domain of Bullous Pemphigoid Antigen1 (BPAG1)==
			<StructureSection load='2iak' size='340' side='right' caption='[[2iak]], [[Resolution\|resolution]] 3.00Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2iak]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IAK FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dst, Bpag1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iak OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2iak RCSB], [http://www.ebi.ac.uk/pdbsum/2iak PDBsum], [http://www.topsan.org/Proteins/NYSGXRC/2iak TOPSAN]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ia/2iak_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Bullous pemphigoid antigen 1 (BPAG1) is a member of the plakin family of proteins. The plakins are multi-domain proteins that have been shown to interact with microtubules, actin filaments and intermediate filaments, as well as proteins found in cellular junctions. These interactions are mediated through different domains on the plakins. The interactions between plakins and components of specialized cell junctions such as desmosomes and hemidesmosomes are mediated through the so-called plakin domain, which is a common feature of the plakins. We report the crystal structure of a stable fragment from BPAG1, residues 226-448, defined by limited proteolysis of the whole plakin domain. The structure, determined by single-wavelength anomalous diffraction phasing from a selenomethionine-substituted crystal at 3.0 A resolution, reveals a tandem pair of triple helical bundles closely related to spectrin repeats. Based on this structure and analysis of sequence conservation, we propose that the architecture of plakin domains is defined by two pairs of spectrin repeats interrupted by a putative Src-Homology 3 (SH3) domain.

	~~{{STRUCTURE_2iak\| PDB=2iak \| SCENE= }}~~		Structural analysis of the plakin domain of bullous pemphigoid antigen1 (BPAG1) suggests that plakins are members of the spectrin superfamily.,Jefferson JJ, Ciatto C, Shapiro L, Liem RK J Mol Biol. 2007 Feb 9;366(1):244-57. Epub 2006 Nov 11. PMID:17161423<ref>PMID:17161423</ref>

	~~===Crystal Structure of~~ a ~~protease resistant fragment~~ of the ~~plakin domain of Bullous Pemphigoid Antigen1 (BPAG1)===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_17161423}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2iak]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2IAK OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:017161423<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Jefferson, J J.]]		[[Category: Jefferson, J J.]]

OCA: Protected "2iak" [edit=sysop:move=sysop]

2013-01-07T12:44:08Z

Protected "2iak" [edit=sysop:move=sysop]

← Older revision	Revision as of 12:44, 7 January 2013
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OCA at 12:44, 7 January 2013

2013-01-07T12:44:06Z

← Older revision		Revision as of 12:44, 7 January 2013
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	~~{{Seed}}~~
	[[Image:2iak.png\|left\|200px]]		[[Image:2iak.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2iak", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
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	{{STRUCTURE_2iak\| PDB=2iak \| SCENE= }}		{{STRUCTURE_2iak\| PDB=2iak \| SCENE= }}

	===Crystal Structure of a protease resistant fragment of the plakin domain of Bullous Pemphigoid Antigen1 (BPAG1)===		===Crystal Structure of a protease resistant fragment of the plakin domain of Bullous Pemphigoid Antigen1 (BPAG1)===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_17161423}}, adds the Publication Abstract to the page~~
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	{{ABSTRACT_PUBMED_17161423}}		{{ABSTRACT_PUBMED_17161423}}

	==About this Structure==		==About this Structure==
	~~2IAK~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAK OCA].		[[2iak]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IAK OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~17161423~~</ref><references group="xtra"/>		<ref group="xtra">PMID:017161423</ref><references group="xtra"/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Jefferson, J J.]]		[[Category: Jefferson, J J.]]
			[[Category: Cell adhesion]]
	[[Category: Spectrin repeat]]		[[Category: Spectrin repeat]]
	[[Category: Triple helical bundle]]		[[Category: Triple helical bundle]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 17:54:23 2009''~~

OCA at 15:54, 16 February 2009

2009-02-16T15:54:25Z

← Older revision		Revision as of 15:54, 16 February 2009
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	==Reference==		==Reference==
	Structural analysis of the plakin domain of bullous pemphigoid antigen1 (BPAG1) suggests that plakins are members of the spectrin superfamily., Jefferson JJ, Ciatto C, Shapiro L, Liem RK, J Mol Biol. 2007 Feb 9;366(1):244-57. Epub 2006 Nov 11. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/17161423 17161423]~~		<ref group="xtra">PMID:17161423</ref><references group="xtra"/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Jefferson, J J.]]		[[Category: Jefferson, J J.]]
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	[[Category: Triple helical bundle]]		[[Category: Triple helical bundle]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun Nov~~ 16 14:41:~~50 2008~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 17:54:23 2009''