2imp - Revision history

OCA at 08:30, 15 November 2023

2023-11-15T08:30:51Z

← Older revision		Revision as of 08:30, 15 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2imp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IMP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IMP FirstGlance]. <br>		<table><tr><td colspan='2'>[[2imp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IMP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IMP FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene>~~, ~~<scene name='pdbligand=NAI:~~1~~,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~OCS~~:~~CYSTEINESULFONIC~~+ACID'>~~OCS~~</scene><~~/td~~></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene>, <scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene>, <scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<~~tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td><~~td class~~=~~"sblockDat"><div style~~=~~'overflow~~: ~~auto; max-height: 3em;~~'>~~[[2hg2\|2hg2]], [[2ilu\|2ilu]]~~</~~div~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2imp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2imp OCA], [https://pdbe.org/2imp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2imp RCSB], [https://www.ebi.ac.uk/pdbsum/2imp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2imp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2imp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2imp OCA], [https://pdbe.org/2imp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2imp RCSB], [https://www.ebi.ac.uk/pdbsum/2imp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2imp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/ALDA_ECOLI ALDA_ECOLI]] Acts on lactaldehyde as well as other aldehydes.		[https://www.uniprot.org/uniprot/ALDA_ECOLI ALDA_ECOLI] Acts on lactaldehyde as well as other aldehydes.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Christianson~~, D W~~]]		[[Category: Christianson DW]]
	[[Category: Costanzo, L Di]]		[[Category: Di Costanzo L]]
	[[Category: Gomez~~, G A]]~~		[[Category: Gomez GA]]
	~~[[Category: Oxidoreductase]]~~
	~~[[Category: Protein-lactate-nadh ternary complex~~]]

OCA at 11:19, 31 March 2021

2021-03-31T11:19:05Z

← Older revision		Revision as of 11:19, 31 March 2021
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	<StructureSection load='2imp' size='340' side='right'caption='[[2imp]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2imp' size='340' side='right'caption='[[2imp]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2imp]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IMP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2IMP FirstGlance]. <br>		<table><tr><td colspan='2'>[[2imp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IMP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IMP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene>, <scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene>, <scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hg2\|2hg2]], [[2ilu\|2ilu]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2hg2\|2hg2]], [[2ilu\|2ilu]]</div></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2imp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2imp OCA], [~~http~~://pdbe.org/2imp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2imp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2imp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2imp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2imp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2imp OCA], [https://pdbe.org/2imp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2imp RCSB], [https://www.ebi.ac.uk/pdbsum/2imp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2imp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/ALDA_ECOLI ALDA_ECOLI]] Acts on lactaldehyde as well as other aldehydes.		[[https://www.uniprot.org/uniprot/ALDA_ECOLI ALDA_ECOLI]] Acts on lactaldehyde as well as other aldehydes.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 06:51, 27 March 2019

2019-03-27T06:51:01Z

← Older revision		Revision as of 06:51, 27 March 2019
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	==Crystal structure of lactaldehyde dehydrogenase from E. coli: the ternary complex with ~~product bound~~ (L)-~~lactate and NADH~~.==		==Crystal structure of lactaldehyde dehydrogenase from E. coli: the ternary complex with lactate (occupancy 0.5) and NADH. Crystals soaked with (L)-Lactate.==
	<StructureSection load='2imp' size='340' side='right' caption='[[2imp]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2imp' size='340' side='right'caption='[[2imp]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2imp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IMP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IMP FirstGlance]. <br>		<table><tr><td colspan='2'>[[2imp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IMP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IMP FirstGlance]. <br>
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	==See Also==		==See Also==
	*[[Aldehyde dehydrogenase\|Aldehyde dehydrogenase]]		*[[Aldehyde dehydrogenase 3D structures\|Aldehyde dehydrogenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
			[[Category: Large Structures]]
	[[Category: Christianson, D W]]		[[Category: Christianson, D W]]
	[[Category: Costanzo, L Di]]		[[Category: Costanzo, L Di]]

OCA at 08:23, 4 July 2018

2018-07-04T08:23:41Z

← Older revision		Revision as of 08:23, 4 July 2018
Line 1:		Line 1:

	==Crystal structure of lactaldehyde dehydrogenase from E. coli: the ternary complex with product bound (L)-lactate and NADH.==		==Crystal structure of lactaldehyde dehydrogenase from E. coli: the ternary complex with product bound (L)-lactate and NADH.==
	<StructureSection load='2imp' size='340' side='right' caption='[[2imp]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2imp' size='340' side='right' caption='[[2imp]], [[Resolution\|resolution]] 2.10Å' scene=''>
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	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hg2\|2hg2]], [[2ilu\|2ilu]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hg2\|2hg2]], [[2ilu\|2ilu]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2imp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2imp OCA], [http://pdbe.org/2imp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2imp RCSB], [http://www.ebi.ac.uk/pdbsum/2imp PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2imp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2imp OCA], [http://pdbe.org/2imp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2imp RCSB], [http://www.ebi.ac.uk/pdbsum/2imp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2imp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/im/2imp_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/im/2imp_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2imp ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:55, 11 September 2015

2015-09-11T07:55:05Z

← Older revision		Revision as of 07:55, 11 September 2015
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	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hg2\|2hg2]], [[2ilu\|2ilu]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hg2\|2hg2]], [[2ilu\|2ilu]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2imp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2imp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2imp RCSB], [http://www.ebi.ac.uk/pdbsum/2imp PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2imp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2imp OCA], [http://pdbe.org/2imp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2imp RCSB], [http://www.ebi.ac.uk/pdbsum/2imp PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2imp" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 13:31, 25 December 2014

2014-12-25T13:31:17Z

← Older revision		Revision as of 13:31, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2imp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IMP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IMP FirstGlance]. <br>		<table><tr><td colspan='2'>[[2imp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IMP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IMP FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene>, <scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene>, <scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hg2\|2hg2]], [[2ilu\|2ilu]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hg2\|2hg2]], [[2ilu\|2ilu]]</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2imp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2imp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2imp RCSB], [http://www.ebi.ac.uk/pdbsum/2imp PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2imp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2imp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2imp RCSB], [http://www.ebi.ac.uk/pdbsum/2imp PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/ALDA_ECOLI ALDA_ECOLI]] Acts on lactaldehyde as well as other aldehydes.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Christianson, D W.]]		[[Category: Christianson, D W]]
	[[Category: Costanzo, L Di.]]		[[Category: Costanzo, L Di]]
	[[Category: Gomez, G A.]]		[[Category: Gomez, G A]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Protein-lactate-nadh ternary complex]]		[[Category: Protein-lactate-nadh ternary complex]]

OCA at 04:43, 29 September 2014

2014-09-29T04:43:44Z

← Older revision		Revision as of 04:43, 29 September 2014
Line 1:		Line 1:
	[[Image:~~2imp~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of lactaldehyde dehydrogenase from E. coli: the ternary complex with product bound (L)-lactate and NADH.==
			<StructureSection load='2imp' size='340' side='right' caption='[[2imp]], [[Resolution\|resolution]] 2.10Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2imp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IMP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IMP FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene>, <scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
			<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hg2\|2hg2]], [[2ilu\|2ilu]]</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2imp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2imp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2imp RCSB], [http://www.ebi.ac.uk/pdbsum/2imp PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/im/2imp_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Aldehyde dehydrogenases catalyze the oxidation of aldehyde substrates to the corresponding carboxylic acids. Lactaldehyde dehydrogenase from Escherichia coli (aldA gene product, P25553) is an NAD(+)-dependent enzyme implicated in the metabolism of l-fucose and l-rhamnose. During the heterologous expression and purification of taxadiene synthase from the Pacific yew, lactaldehyde dehydrogenase from E. coli was identified as a minor (</=5%) side-product subsequent to its unexpected crystallization. Accordingly, we now report the serendipitous crystal structure determination of unliganded lactaldehyde dehydrogenase from E. coli determined by the technique of multiple isomorphous replacement using anomalous scattering at 2.2 A resolution. Additionally, we report the crystal structure of the ternary enzyme complex with products lactate and NADH at 2.1 A resolution, and the crystal structure of the enzyme complex with NADPH at 2.7 A resolution. The structure of the ternary complex reveals that the nicotinamide ring of the cofactor is disordered between two conformations: one with the ring positioned in the active site in the so-called hydrolysis conformation, and another with the ring extended out of the active site into the solvent region, designated the out conformation. This represents the first crystal structure of an aldehyde dehydrogenase-product complex. The active site pocket in which lactate binds is more constricted than that of medium-chain dehydrogenases such as the YdcW gene product of E. coli. The structure of the binary complex with NADPH reveals the first view of the structural basis of specificity for NADH: the negatively charged carboxylate group of E179 destabilizes the binding of the 2'-phosphate group of NADPH sterically and electrostatically, thereby accounting for the lack of enzyme activity with this cofactor.

	~~{{STRUCTURE_2imp\| PDB=2imp \| SCENE= }}~~		Crystal structure of lactaldehyde dehydrogenase from Escherichia coli and inferences regarding substrate and cofactor specificity.,Di Costanzo L, Gomez GA, Christianson DW J Mol Biol. 2007 Feb 16;366(2):481-93. Epub 2006 Nov 10. PMID:17173928<ref>PMID:17173928</ref>

	~~===Crystal structure~~ of ~~lactaldehyde dehydrogenase from E. coli:~~ the ~~ternary complex with product bound (L)-lactate and NADH~~.~~===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_17173928}}~~

	~~==About this Structure==~~
	~~[[2imp]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2IMP OCA].~~

	==See Also==		==See Also==
	*[[Aldehyde dehydrogenase\|Aldehyde dehydrogenase]]		*[[Aldehyde dehydrogenase\|Aldehyde dehydrogenase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:017173928<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Christianson, D W.]]		[[Category: Christianson, D W.]]

OCA at 17:15, 20 October 2012

2012-10-20T17:15:49Z

← Older revision		Revision as of 17:15, 20 October 2012
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	==About this Structure==		==About this Structure==
	[[2imp]] is a 1 chain structure ~~of [[Aldehyde dehydrogenase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IMP OCA].		[[2imp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IMP OCA].

	==See Also==		==See Also==

OCA: Protected "2imp" [edit=sysop:move=sysop]

2012-07-27T00:20:59Z

Protected "2imp" [edit=sysop:move=sysop]

← Older revision	Revision as of 00:20, 27 July 2012
(No difference)

OCA at 00:20, 27 July 2012

2012-07-27T00:20:58Z

← Older revision		Revision as of 00:20, 27 July 2012
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	~~{{Seed}}~~
	[[Image:2imp.png\|left\|200px]]		[[Image:2imp.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2imp", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2imp\| PDB=2imp \| SCENE= }}		{{STRUCTURE_2imp\| PDB=2imp \| SCENE= }}

	===Crystal structure of lactaldehyde dehydrogenase from E. coli: the ternary complex with product bound (L)-lactate and NADH.===		===Crystal structure of lactaldehyde dehydrogenase from E. coli: the ternary complex with product bound (L)-lactate and NADH.===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_17173928}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 17173928 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_17173928}}		{{ABSTRACT_PUBMED_17173928}}

	==About this Structure==		==About this Structure==
	~~2IMP~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IMP OCA].		[[2imp]] is a 1 chain structure of [[Aldehyde dehydrogenase]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IMP OCA].

			==See Also==
			*[[Aldehyde dehydrogenase\|Aldehyde dehydrogenase]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~17173928~~</ref><references group="xtra"/>		<ref group="xtra">PMID:017173928</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Christianson, D W.]]		[[Category: Christianson, D W.]]
	[[Category: Costanzo, L Di.]]		[[Category: Costanzo, L Di.]]
	[[Category: Gomez, G A.]]		[[Category: Gomez, G A.]]
			[[Category: Oxidoreductase]]
	[[Category: Protein-lactate-nadh ternary complex]]		[[Category: Protein-lactate-nadh ternary complex]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 17:28:20 2009''~~