2jev - Revision history

OCA at 14:45, 13 December 2023

2023-12-13T14:45:42Z

← Older revision		Revision as of 14:45, 13 December 2023
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	<StructureSection load='2jev' size='340' side='right'caption='[[2jev]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2jev' size='340' side='right'caption='[[2jev]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2jev]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JEV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JEV FirstGlance]. <br>		<table><tr><td colspan='2'>[[2jev]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JEV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JEV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NHQ:(3R)-27-AMINO-3-HYDROXY-2,2-DIMETHYL-4,8,14-TRIOXO-12-THIA-5,9,15,19,24-PENTAAZAHEPTACOS-1-YL+[(2S,3R,4S,5S)-5-(6-AMINO-9H-PURIN-9-YL)-4-HYDROXY-3-(PHOSPHONOOXY)TETRAHYDROFURAN-2-YL]METHYL+DIHYDROGEN+DIPHOSPHATE'>NHQ</scene~~></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2b3u\|2b3u]], [[2b3v\|2b3v]], [[2b4b\|2b4b]], [[2b4d\|2b4d]], [[2b58\|2b58]], [[2b5g\|2b5g]], [[2f5i\|2f5i]], [[2fxf\|2fxf]], [[2g3t\|2g3t]]</div></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NHQ:(3R)-27-AMINO-3-HYDROXY-2,2-DIMETHYL-4,8,14-TRIOXO-12-THIA-5,9,15,19,24-PENTAAZAHEPTACOS-1-YL+[(2S,3R,4S,5S)-5-(6-AMINO-9H-PURIN-9-YL)-4-HYDROXY-3-(PHOSPHONOOXY)TETRAHYDROFURAN-2-YL]METHYL+DIHYDROGEN+DIPHOSPHATE'>NHQ</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Diamine_N-acetyltransferase Diamine N-acetyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.57 2.3.1.57] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jev OCA], [https://pdbe.org/2jev PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jev RCSB], [https://www.ebi.ac.uk/pdbsum/2jev PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jev ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jev OCA], [https://pdbe.org/2jev PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jev RCSB], [https://www.ebi.ac.uk/pdbsum/2jev PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jev ProSAT]</span></td></tr>
	</table>		</table>
			== Disease ==
			[https://www.uniprot.org/uniprot/SAT1_HUMAN SAT1_HUMAN] Defects in SAT1 may be a cause of keratosis follicularis spinulosa decalvans X-linked (KFSDX) [MIM:[https://omim.org/entry/308800 308800]. A rare disorder affecting the skin and the eye. Affected men show thickening of the skin of the neck, ears, and extremities, especially the palms and soles, loss of eyebrows, eyelashes and beard, thickening of the eyelids with blepharitis and ectropion, and corneal degeneration.<ref>PMID:9341865</ref> <ref>PMID:12215835</ref>
			== Function ==
			[https://www.uniprot.org/uniprot/SAT1_HUMAN SAT1_HUMAN] Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine = spermidine >> spermine > N(1)-acetylspermine > putrescine. This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines. Also involved in the regulation of polyamine transport out of cells. Acts on 1,3-diaminopropane, 1,5-diaminopentane, putrescine, spermidine (forming N(1)- and N(8)-acetylspermidine), spermine, N(1)-acetylspermidine and N(8)-acetylspermidine.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Diamine N-acetyltransferase]]~~		[[Category: Homo sapiens]]
	~~[[Category: Human~~]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Blanchard~~, J S~~]]		[[Category: Blanchard JS]]
	[[Category: Chandler, J]]		[[Category: Chandler J]]
	[[Category: Hegde~~, S S~~]]		[[Category: Hegde SS]]
	[[Category: Vetting~~, M W~~]]		[[Category: Vetting MW]]
	[[Category: Yu, M]]		[[Category: Yu M]]
	~~[[Category: Acetyltransferase]]~~
	~~[[Category: Acyltransferase]]~~
	~~[[Category: Bisubstrate]]~~
	~~[[Category: Gcn5 related n-acetyltransferase]]~~
	~~[[Category: Gnat]]~~
	~~[[Category: Polyamine biosynthesis]]~~
	~~[[Category: Spermidine]]~~
	~~[[Category: Spermine]]~~
	~~[[Category: Transferase~~]]

OCA at 11:26, 31 March 2021

2021-03-31T11:26:34Z

← Older revision		Revision as of 11:26, 31 March 2021
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	<StructureSection load='2jev' size='340' side='right'caption='[[2jev]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2jev' size='340' side='right'caption='[[2jev]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2jev]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JEV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2JEV FirstGlance]. <br>		<table><tr><td colspan='2'>[[2jev]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JEV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JEV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NHQ:(3R)-27-AMINO-3-HYDROXY-2,2-DIMETHYL-4,8,14-TRIOXO-12-THIA-5,9,15,19,24-PENTAAZAHEPTACOS-1-YL+[(2S,3R,4S,5S)-5-(6-AMINO-9H-PURIN-9-YL)-4-HYDROXY-3-(PHOSPHONOOXY)TETRAHYDROFURAN-2-YL]METHYL+DIHYDROGEN+DIPHOSPHATE'>NHQ</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NHQ:(3R)-27-AMINO-3-HYDROXY-2,2-DIMETHYL-4,8,14-TRIOXO-12-THIA-5,9,15,19,24-PENTAAZAHEPTACOS-1-YL+[(2S,3R,4S,5S)-5-(6-AMINO-9H-PURIN-9-YL)-4-HYDROXY-3-(PHOSPHONOOXY)TETRAHYDROFURAN-2-YL]METHYL+DIHYDROGEN+DIPHOSPHATE'>NHQ</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b3u\|2b3u]], [[2b3v\|2b3v]], [[2b4b\|2b4b]], [[2b4d\|2b4d]], [[2b58\|2b58]], [[2b5g\|2b5g]], [[2f5i\|2f5i]], [[2fxf\|2fxf]], [[2g3t\|2g3t]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2b3u\|2b3u]], [[2b3v\|2b3v]], [[2b4b\|2b4b]], [[2b4d\|2b4d]], [[2b58\|2b58]], [[2b5g\|2b5g]], [[2f5i\|2f5i]], [[2fxf\|2fxf]], [[2g3t\|2g3t]]</div></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Diamine_N-acetyltransferase Diamine N-acetyltransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.57 2.3.1.57] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Diamine_N-acetyltransferase Diamine N-acetyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.57 2.3.1.57] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2jev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jev OCA], [~~http~~://pdbe.org/2jev PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2jev RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2jev PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2jev ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jev OCA], [https://pdbe.org/2jev PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jev RCSB], [https://www.ebi.ac.uk/pdbsum/2jev PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jev ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 12:06, 10 May 2019

2019-05-10T12:06:49Z

← Older revision		Revision as of 12:06, 10 May 2019
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	==Crystal structure of human spermine,spermidine acetyltransferase in complex with a bisubstrate analog (N1-acetylspermine-S-CoA).==		==Crystal structure of human spermine,spermidine acetyltransferase in complex with a bisubstrate analog (N1-acetylspermine-S-CoA).==
	<StructureSection load='2jev' size='340' side='right' caption='[[2jev]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2jev' size='340' side='right'caption='[[2jev]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2jev]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JEV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JEV FirstGlance]. <br>		<table><tr><td colspan='2'>[[2jev]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JEV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JEV FirstGlance]. <br>
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	[[Category: Diamine N-acetyltransferase]]		[[Category: Diamine N-acetyltransferase]]
	[[Category: Human]]		[[Category: Human]]
			[[Category: Large Structures]]
	[[Category: Blanchard, J S]]		[[Category: Blanchard, J S]]
	[[Category: Chandler, J]]		[[Category: Chandler, J]]

OCA at 06:47, 11 July 2018

2018-07-11T06:47:53Z

← Older revision		Revision as of 06:47, 11 July 2018
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	==~~CRYSTAL STRUCTURE OF HUMAN SPERMINE~~,~~SPERMIDINE ACETYLTRANSFERASE IN COMPLEX WITH A BISUBSTRATE ANALOG~~ (N1-~~ACETYLSPERMINE~~-S-~~COA~~).==
			==Crystal structure of human spermine,spermidine acetyltransferase in complex with a bisubstrate analog (N1-acetylspermine-S-CoA).==
	<StructureSection load='2jev' size='340' side='right' caption='[[2jev]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2jev' size='340' side='right' caption='[[2jev]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b3u\|2b3u]], [[2b3v\|2b3v]], [[2b4b\|2b4b]], [[2b4d\|2b4d]], [[2b58\|2b58]], [[2b5g\|2b5g]], [[2f5i\|2f5i]], [[2fxf\|2fxf]], [[2g3t\|2g3t]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b3u\|2b3u]], [[2b3v\|2b3v]], [[2b4b\|2b4b]], [[2b4d\|2b4d]], [[2b58\|2b58]], [[2b5g\|2b5g]], [[2f5i\|2f5i]], [[2fxf\|2fxf]], [[2g3t\|2g3t]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diamine_N-acetyltransferase Diamine N-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.57 2.3.1.57] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diamine_N-acetyltransferase Diamine N-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.57 2.3.1.57] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jev OCA], [http://pdbe.org/2jev PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jev RCSB], [http://www.ebi.ac.uk/pdbsum/2jev PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jev OCA], [http://pdbe.org/2jev PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jev RCSB], [http://www.ebi.ac.uk/pdbsum/2jev PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jev ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/je/2jev_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/je/2jev_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 04:39, 10 February 2016

2016-02-10T04:39:53Z

← Older revision		Revision as of 04:39, 10 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jev ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 05:28, 11 September 2015

2015-09-11T05:28:06Z

← Older revision		Revision as of 05:28, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2jev' size='340' side='right' caption='[[2jev]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2jev' size='340' side='right' caption='[[2jev]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2jev]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JEV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JEV FirstGlance]. <br>		<table><tr><td colspan='2'>[[2jev]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JEV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JEV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NHQ:(3R)-27-AMINO-3-HYDROXY-2,2-DIMETHYL-4,8,14-TRIOXO-12-THIA-5,9,15,19,24-PENTAAZAHEPTACOS-1-YL+[(2S,3R,4S,5S)-5-(6-AMINO-9H-PURIN-9-YL)-4-HYDROXY-3-(PHOSPHONOOXY)TETRAHYDROFURAN-2-YL]METHYL+DIHYDROGEN+DIPHOSPHATE'>NHQ</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NHQ:(3R)-27-AMINO-3-HYDROXY-2,2-DIMETHYL-4,8,14-TRIOXO-12-THIA-5,9,15,19,24-PENTAAZAHEPTACOS-1-YL+[(2S,3R,4S,5S)-5-(6-AMINO-9H-PURIN-9-YL)-4-HYDROXY-3-(PHOSPHONOOXY)TETRAHYDROFURAN-2-YL]METHYL+DIHYDROGEN+DIPHOSPHATE'>NHQ</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b3u\|2b3u]], [[2b3v\|2b3v]], [[2b4b\|2b4b]], [[2b4d\|2b4d]], [[2b58\|2b58]], [[2b5g\|2b5g]], [[2f5i\|2f5i]], [[2fxf\|2fxf]], [[2g3t\|2g3t]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b3u\|2b3u]], [[2b3v\|2b3v]], [[2b4b\|2b4b]], [[2b4d\|2b4d]], [[2b58\|2b58]], [[2b5g\|2b5g]], [[2f5i\|2f5i]], [[2fxf\|2fxf]], [[2g3t\|2g3t]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diamine_N-acetyltransferase Diamine N-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.57 2.3.1.57] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diamine_N-acetyltransferase Diamine N-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.57 2.3.1.57] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jev OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jev RCSB], [http://www.ebi.ac.uk/pdbsum/2jev PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jev OCA], [http://pdbe.org/2jev PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jev RCSB], [http://www.ebi.ac.uk/pdbsum/2jev PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2jev" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Diamine N-acetyltransferase]]		[[Category: Diamine N-acetyltransferase]]
	[[Category: ~~Homo sapiens~~]]		[[Category: Human]]
	[[Category: Blanchard, J S]]		[[Category: Blanchard, J S]]
	[[Category: Chandler, J]]		[[Category: Chandler, J]]

OCA at 15:24, 19 January 2015

2015-01-19T15:24:06Z

← Older revision		Revision as of 15:24, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2jev]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JEV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JEV FirstGlance]. <br>		<table><tr><td colspan='2'>[[2jev]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JEV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JEV FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NHQ:(3R)-27-AMINO-3-HYDROXY-2,2-DIMETHYL-4,8,14-TRIOXO-12-THIA-5,9,15,19,24-PENTAAZAHEPTACOS-1-YL+[(2S,3R,4S,5S)-5-(6-AMINO-9H-PURIN-9-YL)-4-HYDROXY-3-(PHOSPHONOOXY)TETRAHYDROFURAN-2-YL]METHYL+DIHYDROGEN+DIPHOSPHATE'>NHQ</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NHQ:(3R)-27-AMINO-3-HYDROXY-2,2-DIMETHYL-4,8,14-TRIOXO-12-THIA-5,9,15,19,24-PENTAAZAHEPTACOS-1-YL+[(2S,3R,4S,5S)-5-(6-AMINO-9H-PURIN-9-YL)-4-HYDROXY-3-(PHOSPHONOOXY)TETRAHYDROFURAN-2-YL]METHYL+DIHYDROGEN+DIPHOSPHATE'>NHQ</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b3u\|2b3u]], [[2b3v\|2b3v]], [[2b4b\|2b4b]], [[2b4d\|2b4d]], [[2b58\|2b58]], [[2b5g\|2b5g]], [[2f5i\|2f5i]], [[2fxf\|2fxf]], [[2g3t\|2g3t]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b3u\|2b3u]], [[2b3v\|2b3v]], [[2b4b\|2b4b]], [[2b4d\|2b4d]], [[2b58\|2b58]], [[2b5g\|2b5g]], [[2f5i\|2f5i]], [[2fxf\|2fxf]], [[2g3t\|2g3t]]</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diamine_N-acetyltransferase Diamine N-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.57 2.3.1.57] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diamine_N-acetyltransferase Diamine N-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.57 2.3.1.57] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jev OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jev RCSB], [http://www.ebi.ac.uk/pdbsum/2jev PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jev OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jev RCSB], [http://www.ebi.ac.uk/pdbsum/2jev PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Diamine N-acetyltransferase]]		[[Category: Diamine N-acetyltransferase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Blanchard, J S.]]		[[Category: Blanchard, J S]]
	[[Category: Chandler, J.]]		[[Category: Chandler, J]]
	[[Category: Hegde, S S.]]		[[Category: Hegde, S S]]
	[[Category: Vetting, M W.]]		[[Category: Vetting, M W]]
	[[Category: Yu, M.]]		[[Category: Yu, M]]
	[[Category: Acetyltransferase]]		[[Category: Acetyltransferase]]
	[[Category: Acyltransferase]]		[[Category: Acyltransferase]]

OCA at 08:41, 30 September 2014

2014-09-30T08:41:53Z

← Older revision		Revision as of 08:41, 30 September 2014
Line 1:		Line 1:
	~~{{STRUCTURE_2jev\| PDB=2jev \| SCENE= }}~~		==CRYSTAL STRUCTURE OF HUMAN SPERMINE,SPERMIDINE ACETYLTRANSFERASE IN COMPLEX WITH A BISUBSTRATE ANALOG (N1-ACETYLSPERMINE-S-COA).==
	===CRYSTAL STRUCTURE OF HUMAN SPERMINE,SPERMIDINE ACETYLTRANSFERASE IN COMPLEX WITH A BISUBSTRATE ANALOG (N1-ACETYLSPERMINE-S-COA).===		<StructureSection load='2jev' size='340' side='right' caption='[[2jev]], [[Resolution\|resolution]] 2.30Å' scene=''>
	~~{{ABSTRACT_PUBMED_17516632}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[2jev]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JEV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JEV FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NHQ:(3R)-27-AMINO-3-HYDROXY-2,2-DIMETHYL-4,8,14-TRIOXO-12-THIA-5,9,15,19,24-PENTAAZAHEPTACOS-1-YL+[(2S,3R,4S,5S)-5-(6-AMINO-9H-PURIN-9-YL)-4-HYDROXY-3-(PHOSPHONOOXY)TETRAHYDROFURAN-2-YL]METHYL+DIHYDROGEN+DIPHOSPHATE'>NHQ</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b3u\|2b3u]], [[2b3v\|2b3v]], [[2b4b\|2b4b]], [[2b4d\|2b4d]], [[2b58\|2b58]], [[2b5g\|2b5g]], [[2f5i\|2f5i]], [[2fxf\|2fxf]], [[2g3t\|2g3t]]</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diamine_N-acetyltransferase Diamine N-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.57 2.3.1.57] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jev FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jev OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jev RCSB], [http://www.ebi.ac.uk/pdbsum/2jev PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/je/2jev_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The N1-acetylation of spermidine and spermine by spermidine/spermine acetyltransferase (SSAT) is a crucial step in the regulation of the cellular polyamine levels in eukaryotic cells. Altered polyamine levels are associated with a variety of cancers as well as other diseases, and key enzymes in the polyamine pathway, including SSAT, are being explored as potential therapeutic drug targets. We have expressed and purified human SSAT in Escherichia coli and characterized its kinetic and chemical mechanism. Initial velocity and inhibition studies support a random sequential mechanism for the enzyme. The bisubstrate analogue, N1-spermine-acetyl-coenzyme A, exhibited linear, competitive inhibition against both substrates with a true Ki of 6 nM. The pH-activity profile was bell-shaped, depending on the ionization state of two groups exhibiting apparent pKa values of 7.27 and 8.87. The three-dimensional crystal structure of SSAT with bound bisubstrate inhibitor was determined at 2.3 A resolution. The structure of the SSAT-spermine-acetyl-coenzyme A complex suggested that Tyr140 acts as general acid and Glu92, through one or more water molecules, acts as the general base during catalysis. On the basis of kinetic properties, pH dependence, and structural information, we propose an acid/base-assisted reaction catalyzed by SSAT, involving a ternary complex.

	~~==About this Structure==~~		Mechanistic and structural analysis of human spermidine/spermine N1-acetyltransferase.,Hegde SS, Chandler J, Vetting MW, Yu M, Blanchard JS Biochemistry. 2007 Jun 19;46(24):7187-95. Epub 2007 May 22. PMID:17516632<ref>PMID:17516632</ref>
	~~[[2jev]] is a 2 chain structure with sequence from [http:~~/~~/en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.~~il/oca-bin~~/~~ocashort?id=2JEV OCA].~~
			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>

	==See Also==		==See Also==
	*[[Spermidine/spermine N-acetyltransferase\|Spermidine/spermine N-acetyltransferase]]		*[[Spermidine/spermine N-acetyltransferase\|Spermidine/spermine N-acetyltransferase]]
			== References ==
	==~~Reference~~==		<references/>
	~~<ref group="xtra">PMID:017516632</ref>~~<references ~~group="xtra"~~/><~~references~~/>		__TOC__
			</StructureSection>
	[[Category: Diamine N-acetyltransferase]]		[[Category: Diamine N-acetyltransferase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]

OCA at 00:14, 25 March 2013

2013-03-25T00:14:04Z

← Older revision		Revision as of 00:14, 25 March 2013
Line 1:		Line 1:
	~~[[Image:2jev.png\|left\|200px]]~~

	{{STRUCTURE_2jev\| PDB=2jev \| SCENE= }}		{{STRUCTURE_2jev\| PDB=2jev \| SCENE= }}

	===CRYSTAL STRUCTURE OF HUMAN SPERMINE,SPERMIDINE ACETYLTRANSFERASE IN COMPLEX WITH A BISUBSTRATE ANALOG (N1-ACETYLSPERMINE-S-COA).===		===CRYSTAL STRUCTURE OF HUMAN SPERMINE,SPERMIDINE ACETYLTRANSFERASE IN COMPLEX WITH A BISUBSTRATE ANALOG (N1-ACETYLSPERMINE-S-COA).===

	{{ABSTRACT_PUBMED_17516632}}		{{ABSTRACT_PUBMED_17516632}}

Line 14:		Line 10:

	==Reference==		==Reference==
	<ref group="xtra">PMID:017516632</ref><references group="xtra"/>		<ref group="xtra">PMID:017516632</ref><references group="xtra"/><references/>
	[[Category: Diamine N-acetyltransferase]]		[[Category: Diamine N-acetyltransferase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]

OCA at 16:21, 20 October 2012

2012-10-20T16:21:49Z

← Older revision		Revision as of 16:21, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[2jev]] is a 2 chain structure ~~of [[Spermidine/spermine N-acetyltransferase]]~~ with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JEV OCA].		[[2jev]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JEV OCA].

	==See Also==		==See Also==