2js2 - Revision history

OCA at 19:04, 29 May 2024

2024-05-29T19:04:43Z

← Older revision		Revision as of 19:04, 29 May 2024
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	==Solution structure of first SH3 domain of adaptor Nck==		==Solution structure of first SH3 domain of adaptor Nck==
	<StructureSection load='2js2' size='340' side='right'caption='[[2js2~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>		<StructureSection load='2js2' size='340' side='right'caption='[[2js2]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2js2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JS2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JS2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2js2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JS2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JS2 FirstGlance]. <br>
	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[2js0\|2js0]]</div></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	~~<tr~~ id~~='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class~~="~~sblockDat~~">~~NCK1, NCK ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2js2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2js2 OCA], [https://pdbe.org/2js2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2js2 RCSB], [https://www.ebi.ac.uk/pdbsum/2js2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2js2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2js2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2js2 OCA], [https://pdbe.org/2js2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2js2 RCSB], [https://www.ebi.ac.uk/pdbsum/2js2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2js2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/NCK1_HUMAN NCK1_HUMAN]] Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.<ref>PMID:10026169</ref> <ref>PMID:16835242</ref> <ref>PMID:17803907</ref>		[https://www.uniprot.org/uniprot/NCK1_HUMAN NCK1_HUMAN] Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.<ref>PMID:10026169</ref> <ref>PMID:16835242</ref> <ref>PMID:17803907</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Human~~]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Carlin~~, C R~~]]		[[Category: Carlin CR]]
	[[Category: Choowongkomon, K]]		[[Category: Choowongkomon K]]
	[[Category: Hake~~, M J~~]]		[[Category: Hake MJ]]
	[[Category: Sonnichsen~~, F D]]~~		[[Category: Sonnichsen FD]]
	~~[[Category: Adaptor]]~~
	~~[[Category: Sh3 domain]]~~
	~~[[Category: Signaling]]~~
	~~[[Category: Signaling protein~~]]

OCA at 07:55, 7 April 2021

2021-04-07T07:55:04Z

← Older revision		Revision as of 07:55, 7 April 2021
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	==Solution structure of first SH3 domain of adaptor Nck==		==Solution structure of first SH3 domain of adaptor Nck==
	<StructureSection load='2js2' size='340' side='right' caption='[[2js2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2js2' size='340' side='right'caption='[[2js2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2js2]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JS2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2JS2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2js2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JS2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JS2 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2js0\|2js0]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2js0\|2js0]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NCK1, NCK ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NCK1, NCK ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2js2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2js2 OCA], [~~http~~://pdbe.org/2js2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2js2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2js2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2js2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2js2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2js2 OCA], [https://pdbe.org/2js2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2js2 RCSB], [https://www.ebi.ac.uk/pdbsum/2js2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2js2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/NCK1_HUMAN NCK1_HUMAN]] Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.<ref>PMID:10026169</ref> <ref>PMID:16835242</ref> <ref>PMID:17803907</ref>		[[https://www.uniprot.org/uniprot/NCK1_HUMAN NCK1_HUMAN]] Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.<ref>PMID:10026169</ref> <ref>PMID:16835242</ref> <ref>PMID:17803907</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
			[[Category: Large Structures]]
	[[Category: Carlin, C R]]		[[Category: Carlin, C R]]
	[[Category: Choowongkomon, K]]		[[Category: Choowongkomon, K]]

OCA at 06:42, 11 July 2018

2018-07-11T06:42:51Z

← Older revision		Revision as of 06:42, 11 July 2018
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	==Solution structure of first SH3 domain of adaptor Nck==		==Solution structure of first SH3 domain of adaptor Nck==
	<StructureSection load='2js2' size='340' side='right' caption='[[2js2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2js2' size='340' side='right' caption='[[2js2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
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	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2js0\|2js0]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2js0\|2js0]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NCK1, NCK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NCK1, NCK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2js2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2js2 OCA], [http://pdbe.org/2js2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2js2 RCSB], [http://www.ebi.ac.uk/pdbsum/2js2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2js2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2js2 OCA], [http://pdbe.org/2js2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2js2 RCSB], [http://www.ebi.ac.uk/pdbsum/2js2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2js2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/js/2js2_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/js/2js2_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2js2 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 15:10, 11 September 2015

2015-09-11T15:10:57Z

← Older revision		Revision as of 15:10, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2js2' size='340' side='right' caption='[[2js2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2js2' size='340' side='right' caption='[[2js2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2js2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JS2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JS2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2js2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JS2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JS2 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2js0\|2js0]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2js0\|2js0]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NCK1, NCK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NCK1, NCK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2js2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2js2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2js2 RCSB], [http://www.ebi.ac.uk/pdbsum/2js2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2js2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2js2 OCA], [http://pdbe.org/2js2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2js2 RCSB], [http://www.ebi.ac.uk/pdbsum/2js2 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2js2" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Homo sapiens~~]]		[[Category: Human]]
	[[Category: Carlin, C R]]		[[Category: Carlin, C R]]
	[[Category: Choowongkomon, K]]		[[Category: Choowongkomon, K]]

OCA at 18:12, 25 December 2014

2014-12-25T18:12:38Z

← Older revision		Revision as of 18:12, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2js2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JS2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JS2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2js2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JS2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JS2 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2js0\|2js0]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2js0\|2js0]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NCK1, NCK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NCK1, NCK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2js2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2js2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2js2 RCSB], [http://www.ebi.ac.uk/pdbsum/2js2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2js2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2js2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2js2 RCSB], [http://www.ebi.ac.uk/pdbsum/2js2 PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/NCK1_HUMAN NCK1_HUMAN]] Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.<ref>PMID:10026169</ref> <ref>PMID:16835242</ref> <ref>PMID:17803907</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Carlin, C R.]]		[[Category: Carlin, C R]]
	[[Category: Choowongkomon, K.]]		[[Category: Choowongkomon, K]]
	[[Category: Hake, M J.]]		[[Category: Hake, M J]]
	[[Category: Sonnichsen, F D.]]		[[Category: Sonnichsen, F D]]
	[[Category: Adaptor]]		[[Category: Adaptor]]
	[[Category: Sh3 domain]]		[[Category: Sh3 domain]]
	[[Category: Signaling]]		[[Category: Signaling]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]

OCA at 09:23, 30 September 2014

2014-09-30T09:23:27Z

← Older revision		Revision as of 09:23, 30 September 2014
Line 1:		Line 1:
	[[Image:~~2js2~~.~~png~~\|~~left~~\|~~200px~~]]		==Solution structure of first SH3 domain of adaptor Nck==
			<StructureSection load='2js2' size='340' side='right' caption='[[2js2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2js2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JS2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JS2 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2js0\|2js0]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NCK1, NCK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2js2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2js2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2js2 RCSB], [http://www.ebi.ac.uk/pdbsum/2js2 PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/js/2js2_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Nck is a ubiquitously expressed adaptor protein containing Src homology 2 (SH2) and Src homology 3 (SH3) domains. It integrates downstream effector proteins with cell membrane receptors, such as the epidermal growth factor receptor (EGFR). EGFR plays a critical role in cellular proliferation and differentiation. The 45-residue juxtamembrane domain of EGFR (JM), located between the transmembrane and kinase domains, regulates receptor activation and trafficking to the basolateral membrane of polarized epithelia through a proline-rich motif that resembles a consensus SH3 domain binding site. We demonstrate here that the JM region can bind to Nck, showing a notable binding preference for the second SH3 domain. To elucidate the structural determinants for this interaction, we have determined the NMR solution structures of both the first and second Nck SH3 domains (Nck1-1 and Nck1-2). These domains adopt a canonical SH3 beta-barrel-like fold, containing five antiparallel strands separated by three loop regions and one 3 10-helical turn. Chemical shift perturbation studies have identified the residues that form the binding cleft of Nck1-2, which are primarily located in the RT and n-Src loops. JM binds to Nck1-2 with an affinity of approximately 80 microM through a positively charged sequence near the N-terminus, as opposed to the polyproline sequence. The two Nck SH3 domains exhibit both steric and electrostatic differences in their RT-Src and n-Src loops, and a model of the Nck1-2 domain complexed with the JM highlights the factors that define the putative binding mode for this ligand.

	~~{{STRUCTURE_2js2\| PDB=2js2 \| SCENE= }}~~		Specificity Determinants of a Novel Nck Interaction with the Juxtamembrane Domain of the Epidermal Growth Factor Receptor(,).,Hake MJ, Choowongkomon K, Kostenko O, Carlin CR, Sonnichsen FD Biochemistry. 2008 Feb 13;. PMID:18269246<ref>PMID:18269246</ref>

	~~===Solution structure of first SH3 domain~~ of ~~adaptor Nck===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_18269246}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2js2]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2JS2 OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:018269246<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Carlin, C R.]]		[[Category: Carlin, C R.]]

OCA: Protected "2js2" [edit=sysop:move=sysop]

2013-01-07T14:17:29Z

Protected "2js2" [edit=sysop:move=sysop]

← Older revision	Revision as of 14:17, 7 January 2013
(No difference)

OCA at 14:17, 7 January 2013

2013-01-07T14:17:27Z

← Older revision		Revision as of 14:17, 7 January 2013
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	~~{{Seed}}~~
	[[Image:2js2.png\|left\|200px]]		[[Image:2js2.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2js2", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2js2\| PDB=2js2 \| SCENE= }}		{{STRUCTURE_2js2\| PDB=2js2 \| SCENE= }}

	===Solution structure of first SH3 domain of adaptor Nck===		===Solution structure of first SH3 domain of adaptor Nck===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_18269246}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 18269246 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_18269246}}		{{ABSTRACT_PUBMED_18269246}}

	==About this Structure==		==About this Structure==
	~~2JS2~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JS2 OCA].		[[2js2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JS2 OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~18269246~~</ref><references group="xtra"/>		<ref group="xtra">PMID:018269246</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Carlin, C R.]]		[[Category: Carlin, C R.]]
Line 33:		Line 21:
	[[Category: Signaling]]		[[Category: Signaling]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 01:37:07 2009''~~

OCA at 23:37, 17 February 2009

2009-02-17T23:37:10Z

← Older revision		Revision as of 23:37, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
	2JS2 is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JS2 OCA].		2JS2 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JS2 OCA].

	==Reference==		==Reference==
	Specificity Determinants of a Novel Nck Interaction with the Juxtamembrane Domain of the Epidermal Growth Factor Receptor(,)., Hake MJ, Choowongkomon K, Kostenko O, Carlin CR, Sonnichsen FD, Biochemistry. 2008 Feb 13;. PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~18269246 18269246]~~		<ref group="xtra">PMID:18269246</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	~~[[Category: Single protein]]~~
	[[Category: Carlin, C R.]]		[[Category: Carlin, C R.]]
	[[Category: Choowongkomon, K.]]		[[Category: Choowongkomon, K.]]
Line 35:		Line 34:
	[[Category: Signaling protein]]		[[Category: Signaling protein]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 08~~:39:~~27 2008~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 01:37:07 2009''

OCA at 05:39, 29 July 2008

2008-07-29T05:39:32Z

← Older revision		Revision as of 05:39, 29 July 2008
Line 1:		Line 1:
	[[Image:2js2.~~jpg~~\|left\|200px]]		{{Seed}}
			[[Image:2js2.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_2js2\| PDB=2js2 \| SCENE= }}		{{STRUCTURE_2js2\| PDB=2js2 \| SCENE= }}

	~~'''~~Solution structure of first SH3 domain of adaptor Nck~~'''~~		===Solution structure of first SH3 domain of adaptor Nck===


	~~==Overview==~~		<!--
	Nck is a ubiquitously expressed adaptor protein containing Src homology 2 (SH2) and Src homology 3 (SH3) domains. It integrates downstream effector proteins with cell membrane receptors, such as the epidermal growth factor receptor (EGFR). EGFR plays a critical role in cellular proliferation and differentiation. The ~~45-residue juxtamembrane domain of EGFR (JM)~~, ~~located between the transmembrane and kinase domains~~, regulates receptor activation and trafficking to the basolateral membrane of polarized epithelia through a proline-rich motif that resembles a consensus SH3 domain binding site. We demonstrate here that the ~~JM region can bind~~ to ~~Nck, showing a notable binding preference for~~ the ~~second SH3 domain. To elucidate the structural determinants for this interaction, we have determined the NMR solution structures of both the first and second Nck SH3 domains~~ (~~Nck1-1 and Nck1-2)~~. ~~These domains adopt a canonical SH3 beta-barrel-like fold, containing five antiparallel strands separated by three loop regions and one 3 10-helical turn~~. ~~Chemical shift perturbation studies have identified the residues that form the binding cleft of Nck1-2~~, ~~which are primarily located in~~ the ~~RT and n-Src loops~~. ~~JM binds to Nck1~~-~~2 with an affinity of approximately 80 microM through a positively charged sequence near the N~~-terminus, as opposed to the polyproline sequence. The two Nck SH3 domains exhibit both steric and electrostatic differences in their RT-Src and n-Src loops, and a model of the Nck1-2 domain complexed with the JM highlights the factors that define the putative binding mode for this ligand.		The line below this paragraph, {{ABSTRACT_PUBMED_18269246}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 18269246 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_18269246}}

	==About this Structure==		==About this Structure==
	2JS2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JS2 OCA].		2JS2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JS2 OCA].

	==Reference==		==Reference==
Line 30:		Line 34:
	[[Category: Signaling]]		[[Category: Signaling]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]
	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 09~~:14:39 2008''
			''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:39:27 2008''