2kr2 - Revision history

OCA at 06:47, 1 May 2024

2024-05-01T06:47:57Z

← Older revision		Revision as of 06:47, 1 May 2024
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	==Xenopus laevis malectin complexed with maltose (Glcalpha1-4Glc)==		==Xenopus laevis malectin complexed with maltose (Glcalpha1-4Glc)==
	<StructureSection load='2kr2' size='340' side='right'caption='[[2kr2~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>		<StructureSection load='2kr2' size='340' side='right'caption='[[2kr2]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2kr2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~African_clawed_frog African clawed frog~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KR2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KR2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2kr2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KR2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KR2 FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[2jwp\|2jwp]], [[2k46\|2k46]]~~</~~div~~><~~/td~~></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900018:beta-maltose'>PRD_900018</scene></td></tr>
	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></~~td><td class="sblockDat"~~>~~mlec, mlec-A ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 African clawed frog])~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kr2 OCA], [https://pdbe.org/2kr2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kr2 RCSB], [https://www.ebi.ac.uk/pdbsum/2kr2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kr2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kr2 OCA], [https://pdbe.org/2kr2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kr2 RCSB], [https://www.ebi.ac.uk/pdbsum/2kr2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kr2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/MLECA_XENLA MLECA_XENLA]] Carbohydate-binding protein with a strong ligand preference for Glc2-N-glycan. May play a role in the early steps of protein N-glycosylation. Can bind di- or higher oligomers but not monomers of glucose, including maltose, maltotriose, maltotetraose, maltoheptaose, nigerose, kojibose, cellobiose and isomaltose, although based on their subcellular locations, these are unlikely to all be physiological ligands.<ref>PMID:18524852</ref>		[https://www.uniprot.org/uniprot/MLECA_XENLA MLECA_XENLA] Carbohydate-binding protein with a strong ligand preference for Glc2-N-glycan. May play a role in the early steps of protein N-glycosylation. Can bind di- or higher oligomers but not monomers of glucose, including maltose, maltotriose, maltotetraose, maltoheptaose, nigerose, kojibose, cellobiose and isomaltose, although based on their subcellular locations, these are unlikely to all be physiological ligands.<ref>PMID:18524852</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kr2 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kr2 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The endoplasmic reticulum malectin is a highly conserved protein in the animal kingdom that has no counterpart so far in lower organisms. We recently determined the structure of its conserved domain and found a highly selective binding to Glc(2)Man(9)GlcNAc(2), an intermediate of N-glycosylation. In our quest for putative ligands during the initial characterization of the protein, we noticed that the malectin domain is highly specific for diglucosides but quite tolerant towards the linkage of the glucosidic bond. To understand the molecular requirements for the observed promiscuity of the malectin domain, here we analyze the binding to a range of diglucosides through comparison of the protein chemical shift perturbation patterns and the saturation transfer difference spectra of the ligands including two maltose-mimicking drugs. A comparison of the maltose-bound structure of the malectin domain with the complex of the native ligand nigerose reveals why malectin is able to tolerate such a diversity of ligands.

	Analysis of the specific interactions between the lectin domain of malectin and diglucosides.,Schallus T, Feher K, Sternberg U, Rybin V, Muhle-Goll C Glycobiology. 2010 Aug;20(8):1010-20. Epub 2010 Apr 26. PMID:20466650<ref>PMID:20466650</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2kr2" style="background-color:#fffaf0;"></div>~~
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: African clawed frog]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Feher, K]]		[[Category: Xenopus laevis]]
	[[Category: Muhle-Goll, C]]		[[Category: Feher K]]
	[[Category: Schallus, T]]		[[Category: Muhle-Goll C]]
	~~[[Category: Carbohydrate binding protein]]~~		[[Category: Schallus T]]
	~~[[Category: Carbohydrate metabolism]]~~
	~~[[Category: Endoplasmic reticulum]]~~
	~~[[Category: Glycoprotein]]~~
	~~[[Category: Lectin/carbohydrate]]~~
	~~[[Category: Membrane]]~~
	~~[[Category: Transmembrane~~]]

OCA at 07:30, 14 April 2021

2021-04-14T07:30:47Z

← Older revision		Revision as of 07:30, 14 April 2021
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	==Xenopus laevis malectin complexed with maltose (Glcalpha1-4Glc)==		==Xenopus laevis malectin complexed with maltose (Glcalpha1-4Glc)==
	<StructureSection load='2kr2' size='340' side='right' caption='[[2kr2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2kr2' size='340' side='right'caption='[[2kr2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2kr2]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/African_clawed_frog African clawed frog]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KR2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2KR2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2kr2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/African_clawed_frog African clawed frog]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KR2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KR2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jwp\|2jwp]], [[2k46\|2k46]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2jwp\|2jwp]], [[2k46\|2k46]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mlec, mlec-A ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 African clawed frog])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mlec, mlec-A ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 African clawed frog])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2kr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kr2 OCA], [~~http~~://pdbe.org/2kr2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2kr2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2kr2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2kr2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kr2 OCA], [https://pdbe.org/2kr2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kr2 RCSB], [https://www.ebi.ac.uk/pdbsum/2kr2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kr2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/MLECA_XENLA MLECA_XENLA]] Carbohydate-binding protein with a strong ligand preference for Glc2-N-glycan. May play a role in the early steps of protein N-glycosylation. Can bind di- or higher oligomers but not monomers of glucose, including maltose, maltotriose, maltotetraose, maltoheptaose, nigerose, kojibose, cellobiose and isomaltose, although based on their subcellular locations, these are unlikely to all be physiological ligands.<ref>PMID:18524852</ref>		[[https://www.uniprot.org/uniprot/MLECA_XENLA MLECA_XENLA]] Carbohydate-binding protein with a strong ligand preference for Glc2-N-glycan. May play a role in the early steps of protein N-glycosylation. Can bind di- or higher oligomers but not monomers of glucose, including maltose, maltotriose, maltotetraose, maltoheptaose, nigerose, kojibose, cellobiose and isomaltose, although based on their subcellular locations, these are unlikely to all be physiological ligands.<ref>PMID:18524852</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: African clawed frog]]		[[Category: African clawed frog]]
			[[Category: Large Structures]]
	[[Category: Feher, K]]		[[Category: Feher, K]]
	[[Category: Muhle-Goll, C]]		[[Category: Muhle-Goll, C]]

OCA at 08:19, 25 July 2018

2018-07-25T08:19:45Z

← Older revision		Revision as of 08:19, 25 July 2018
Line 1:		Line 1:

	==Xenopus laevis malectin complexed with maltose (Glcalpha1-4Glc)==		==Xenopus laevis malectin complexed with maltose (Glcalpha1-4Glc)==
	<StructureSection load='2kr2' size='340' side='right' caption='[[2kr2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2kr2' size='340' side='right' caption='[[2kr2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jwp\|2jwp]], [[2k46\|2k46]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jwp\|2jwp]], [[2k46\|2k46]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mlec, mlec-A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 African clawed frog])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mlec, mlec-A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 African clawed frog])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kr2 OCA], [http://pdbe.org/2kr2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kr2 RCSB], [http://www.ebi.ac.uk/pdbsum/2kr2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kr2 OCA], [http://pdbe.org/2kr2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kr2 RCSB], [http://www.ebi.ac.uk/pdbsum/2kr2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2kr2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kr/2kr2_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kr/2kr2_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kr2 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 01:53, 10 September 2015

2015-09-10T01:53:50Z

← Older revision		Revision as of 01:53, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2kr2' size='340' side='right' caption='[[2kr2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2kr2' size='340' side='right' caption='[[2kr2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2kr2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Xenopus_laevis Xenopus laevis~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KR2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KR2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2kr2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/African_clawed_frog African clawed frog]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KR2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KR2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jwp\|2jwp]], [[2k46\|2k46]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jwp\|2jwp]], [[2k46\|2k46]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mlec, mlec-A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 ~~Xenopus laevis~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mlec, mlec-A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 African clawed frog])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kr2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kr2 RCSB], [http://www.ebi.ac.uk/pdbsum/2kr2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kr2 OCA], [http://pdbe.org/2kr2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kr2 RCSB], [http://www.ebi.ac.uk/pdbsum/2kr2 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2kr2" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Xenopus laevis~~]]		[[Category: African clawed frog]]
	[[Category: Feher, K]]		[[Category: Feher, K]]
	[[Category: Muhle-Goll, C]]		[[Category: Muhle-Goll, C]]

OCA at 15:25, 24 December 2014

2014-12-24T15:25:12Z

← Older revision		Revision as of 15:25, 24 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kr2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kr2 RCSB], [http://www.ebi.ac.uk/pdbsum/2kr2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kr2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kr2 RCSB], [http://www.ebi.ac.uk/pdbsum/2kr2 PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/MLECA_XENLA MLECA_XENLA]] Carbohydate-binding protein with a strong ligand preference for Glc2-N-glycan. May play a role in the early steps of protein N-glycosylation. Can bind di- or higher oligomers but not monomers of glucose, including maltose, maltotriose, maltotetraose, maltoheptaose, nigerose, kojibose, cellobiose and isomaltose, although based on their subcellular locations, these are unlikely to all be physiological ligands.<ref>PMID:18524852</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Xenopus laevis]]		[[Category: Xenopus laevis]]
	[[Category: Feher, K.]]		[[Category: Feher, K]]
	[[Category: Muhle-Goll, C.]]		[[Category: Muhle-Goll, C]]
	[[Category: Schallus, T.]]		[[Category: Schallus, T]]
	[[Category: Carbohydrate binding protein]]		[[Category: Carbohydrate binding protein]]
	[[Category: Carbohydrate metabolism]]		[[Category: Carbohydrate metabolism]]

OCA at 15:29, 12 October 2014

2014-10-12T15:29:54Z

← Older revision		Revision as of 15:29, 12 October 2014
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	[[Image:~~2kr2~~.~~png~~\|~~left~~\|~~200px~~]]		==Xenopus laevis malectin complexed with maltose (Glcalpha1-4Glc)==
			<StructureSection load='2kr2' size='340' side='right' caption='[[2kr2]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2kr2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KR2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KR2 FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jwp\|2jwp]], [[2k46\|2k46]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mlec, mlec-A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 Xenopus laevis])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kr2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kr2 RCSB], [http://www.ebi.ac.uk/pdbsum/2kr2 PDBsum]</span></td></tr>
			</table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kr/2kr2_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The endoplasmic reticulum malectin is a highly conserved protein in the animal kingdom that has no counterpart so far in lower organisms. We recently determined the structure of its conserved domain and found a highly selective binding to Glc(2)Man(9)GlcNAc(2), an intermediate of N-glycosylation. In our quest for putative ligands during the initial characterization of the protein, we noticed that the malectin domain is highly specific for diglucosides but quite tolerant towards the linkage of the glucosidic bond. To understand the molecular requirements for the observed promiscuity of the malectin domain, here we analyze the binding to a range of diglucosides through comparison of the protein chemical shift perturbation patterns and the saturation transfer difference spectra of the ligands including two maltose-mimicking drugs. A comparison of the maltose-bound structure of the malectin domain with the complex of the native ligand nigerose reveals why malectin is able to tolerate such a diversity of ligands.

	~~{{STRUCTURE_2kr2\| PDB=2kr2 \| SCENE= }}~~		Analysis of the specific interactions between the lectin domain of malectin and diglucosides.,Schallus T, Feher K, Sternberg U, Rybin V, Muhle-Goll C Glycobiology. 2010 Aug;20(8):1010-20. Epub 2010 Apr 26. PMID:20466650<ref>PMID:20466650</ref>

	~~===Xenopus laevis malectin complexed with maltose (Glcalpha1-4Glc)===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_20466650}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2kr2]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Xenopus_laevis Xenopus laevis]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2KR2 OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:020466650<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Xenopus laevis]]		[[Category: Xenopus laevis]]
	[[Category: Feher, K.]]		[[Category: Feher, K.]]

OCA: Protected "2kr2" [edit=sysop:move=sysop]

2013-01-07T13:49:32Z

Protected "2kr2" [edit=sysop:move=sysop]

← Older revision	Revision as of 13:49, 7 January 2013
(No difference)

OCA at 13:49, 7 January 2013

2013-01-07T13:49:29Z

← Older revision		Revision as of 13:49, 7 January 2013
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	~~{{Seed}}~~
	[[Image:2kr2.png\|left\|200px]]		[[Image:2kr2.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2kr2", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2kr2\| PDB=2kr2 \| SCENE= }}		{{STRUCTURE_2kr2\| PDB=2kr2 \| SCENE= }}

	===Xenopus laevis malectin complexed with maltose (Glcalpha1-4Glc)===		===Xenopus laevis malectin complexed with maltose (Glcalpha1-4Glc)===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_20466650}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 20466650 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_20466650}}		{{ABSTRACT_PUBMED_20466650}}

	==About this Structure==		==About this Structure==
	~~2KR2~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KR2 OCA].		[[2kr2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KR2 OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~20466650~~</ref><references group="xtra"/>		<ref group="xtra">PMID:020466650</ref><references group="xtra"/>
	[[Category: Xenopus laevis]]		[[Category: Xenopus laevis]]
	[[Category: Feher, K.]]		[[Category: Feher, K.]]
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	[[Category: Membrane]]		[[Category: Membrane]]
	[[Category: Transmembrane]]		[[Category: Transmembrane]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 28 11:58:16 2010''~~

OCA at 07:51, 28 July 2010

2010-07-28T07:51:34Z

← Older revision		Revision as of 07:51, 28 July 2010
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	{{Seed}}		{{Seed}}
	[[Image:2kr2.~~jpg~~\|left\|200px]]		[[Image:2kr2.png\|left\|200px]]

	<!--		<!--
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	[[Category: Transmembrane]]		[[Category: Transmembrane]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul ~~14 16~~:15:32 2010''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 28 11:58:16 2010''

OCA at 12:09, 14 July 2010

2010-07-14T12:09:23Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+{{Seed}}
-The entry 2kr2 is ON HOLD  until Paper Publication
+<!--
-Authors: Schallus, T., Feher, K., Muhle-Goll, C.
+===Xenopus laevis malectin complexed with maltose (Glcalpha1-4Glc)===
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul  7 08:20:26 2010''
+<!--