2l8a - Revision history

OCA at 06:54, 1 May 2024

2024-05-01T06:54:23Z

← Older revision		Revision as of 06:54, 1 May 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2l8a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L8A FirstGlance]. <br>		<table><tr><td colspan='2'>[[2l8a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L8A FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8a OCA], [https://pdbe.org/2l8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l8a RCSB], [https://www.ebi.ac.uk/pdbsum/2l8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l8a ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8a OCA], [https://pdbe.org/2l8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l8a RCSB], [https://www.ebi.ac.uk/pdbsum/2l8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l8a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/GUN2_BACSU GUN2_BACSU]		[https://www.uniprot.org/uniprot/GUN2_BACSU GUN2_BACSU]
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Cellulases participate in a number of biological events such as plant cell wall remodeling, nematode parasitism and microbial carbon uptake. Their ability to depolymerize crystalline cellulose is of great biotechnological interest for environmentally-compatible production of fuels from lignocellulosic biomass. However, industrial use of cellulases is somewhat limited both by their low catalytic efficiency and stability. In this work, we conducted a detailed functional and structural characterization of the thermostable cellulase 5A from Bacillus subtilis (BsCel5A), which consists of a GH5 catalytic domain fused to a family 3 carbohydrate-binding module (CBM3). NMR structural analysis revealed that the Bacillus CBM3 represents a new subfamily, which lacks the classical calcium-binding motif and variations in NMR frequencies in the presence of cellopentaose showed the importance of polar residues in the carbohydrate interaction. Together with the catalytic domain, the CBM3 forms a large planar surface for cellulose recognition, which conducts the substrate in a proper conformation to the active site and increases enzymatic efficiency. Notably, the manganese ion demonstrated to have a hyper-stabilizing effect on BsCel5A and by using deletion constructs and X-ray crystallography, we determined that this effect maps to a negatively charged motif located at the opposite face of the catalytic site.

	Dissecting structure-function-stability relationships of a thermostable GH5-CBM3 cellulase from Bacillus subtilis 168.,Santos C, Paiva J, Sforca M, Neves J, Navarro R, Cota J, Akao P, Hoffmam ZB, Meza A, Smetana J, Nogueira M, Polikarpov I, Xavier-Neto J, Squina F, Ward RJ, Ruller R, Zeri A, Murakami MT Biochem J. 2011 Sep 1. PMID:21880019<ref>PMID:21880019</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2l8a" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Glucanase 3D structures\|Glucanase 3D structures]]		*[[Glucanase 3D structures\|Glucanase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:25, 18 January 2023

2023-01-18T08:25:58Z

← Older revision		Revision as of 08:25, 18 January 2023
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	==Structure of a novel CBM3 lacking the calcium-binding site==		==Structure of a novel CBM3 lacking the calcium-binding site==
	<StructureSection load='2l8a' size='340' side='right'caption='[[2l8a~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>		<StructureSection load='2l8a' size='340' side='right'caption='[[2l8a]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2l8a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"vibrio_subtilis"_ehrenberg_1835 "vibrio~~ subtilis~~" ehrenberg 1835~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L8A FirstGlance]. <br>		<table><tr><td colspan='2'>[[2l8a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L8A FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3pzu\|3pzu]], [[3pzv\|3pzv]], [[3pzt\|3pzt]]</div></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8a OCA], [https://pdbe.org/2l8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l8a RCSB], [https://www.ebi.ac.uk/pdbsum/2l8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l8a ProSAT]</span></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eglS, bglC, gld, BSU18130 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8a OCA], [https://pdbe.org/2l8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l8a RCSB], [https://www.ebi.ac.uk/pdbsum/2l8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l8a ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/GUN2_BACSU GUN2_BACSU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Vibrio~~ subtilis ~~ehrenberg 1835]]~~		[[Category: Bacillus subtilis]]
	~~[[Category: Cellulase~~]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Meza~~, A N~~]]		[[Category: Meza AN]]
	[[Category: Murakami~~, M T~~]]		[[Category: Murakami MT]]
	[[Category: Navarro~~, R Z~~]]		[[Category: Navarro RZ]]
	[[Category: Neves~~, J L~~]]		[[Category: Neves JL]]
	[[Category: Paiva~~, J H~~]]		[[Category: Paiva JH]]
	[[Category: Santos~~, C R~~]]		[[Category: Santos CR]]
	[[Category: Sforca~~, M L~~]]		[[Category: Sforca ML]]
	[[Category: Zeri~~, A C]]~~		[[Category: Zeri AC]]
	~~[[Category: Carbohydrate-binding module]]~~
	~~[[Category: Family 3]]~~
	~~[[Category: Hydrolase~~]]

OCA at 07:38, 14 April 2021

2021-04-14T07:38:09Z

← Older revision		Revision as of 07:38, 14 April 2021
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	==Structure of a novel CBM3 lacking the calcium-binding site==		==Structure of a novel CBM3 lacking the calcium-binding site==
	<StructureSection load='2l8a' size='340' side='right' caption='[[2l8a]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2l8a' size='340' side='right'caption='[[2l8a]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2l8a]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8A OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2L8A FirstGlance]. <br>		<table><tr><td colspan='2'>[[2l8a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L8A FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pzu\|3pzu]], [[3pzv\|3pzv]], [[3pzt\|3pzt]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3pzu\|3pzu]], [[3pzv\|3pzv]], [[3pzt\|3pzt]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eglS, bglC, gld, BSU18130 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eglS, bglC, gld, BSU18130 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2l8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8a OCA], [~~http~~://pdbe.org/2l8a PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2l8a RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2l8a PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2l8a ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8a OCA], [https://pdbe.org/2l8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l8a RCSB], [https://www.ebi.ac.uk/pdbsum/2l8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l8a ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	==See Also==		==See Also==
	*[[Glucanase\|Glucanase]]		*[[Glucanase 3D structures\|Glucanase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Vibrio subtilis ehrenberg 1835]]		[[Category: Vibrio subtilis ehrenberg 1835]]
	[[Category: Cellulase]]		[[Category: Cellulase]]
			[[Category: Large Structures]]
	[[Category: Meza, A N]]		[[Category: Meza, A N]]
	[[Category: Murakami, M T]]		[[Category: Murakami, M T]]

OCA at 19:35, 1 August 2018

2018-08-01T19:35:05Z

← Older revision		Revision as of 19:35, 1 August 2018
Line 1:		Line 1:

	==Structure of a novel CBM3 lacking the calcium-binding site==		==Structure of a novel CBM3 lacking the calcium-binding site==
	<StructureSection load='2l8a' size='340' side='right' caption='[[2l8a]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2l8a' size='340' side='right' caption='[[2l8a]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2l8a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"~~bacillus_globigii~~"~~_migula_1900~~ "~~bacillus globigii~~" ~~migula 1900~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L8A FirstGlance]. <br>		<table><tr><td colspan='2'>[[2l8a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L8A FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pzu\|3pzu]], [[3pzv\|3pzv]], [[3pzt\|3pzt]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pzu\|3pzu]], [[3pzv\|3pzv]], [[3pzt\|3pzt]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eglS, bglC, gld, BSU18130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "~~Bacillus globigii~~" ~~Migula 1900~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eglS, bglC, gld, BSU18130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8a OCA], [http://pdbe.org/2l8a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l8a RCSB], [http://www.ebi.ac.uk/pdbsum/2l8a PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8a OCA], [http://pdbe.org/2l8a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l8a RCSB], [http://www.ebi.ac.uk/pdbsum/2l8a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2l8a ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bacillus globigii migula 1900~~]]		[[Category: Vibrio subtilis ehrenberg 1835]]
	[[Category: Cellulase]]		[[Category: Cellulase]]
	[[Category: Meza, A N]]		[[Category: Meza, A N]]

OCA at 03:57, 11 September 2015

2015-09-11T03:57:42Z

← Older revision		Revision as of 03:57, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2l8a' size='340' side='right' caption='[[2l8a]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2l8a' size='340' side='right' caption='[[2l8a]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2l8a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_subtilis Bacillus subtilis~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L8A FirstGlance]. <br>		<table><tr><td colspan='2'>[[2l8a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L8A FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pzu\|3pzu]], [[3pzv\|3pzv]], [[3pzt\|3pzt]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pzu\|3pzu]], [[3pzv\|3pzv]], [[3pzt\|3pzt]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eglS, bglC, gld, BSU18130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus ~~subtilis~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eglS, bglC, gld, BSU18130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l8a RCSB], [http://www.ebi.ac.uk/pdbsum/2l8a PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8a OCA], [http://pdbe.org/2l8a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l8a RCSB], [http://www.ebi.ac.uk/pdbsum/2l8a PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2l8a" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Bacillus ~~subtilis~~]]		[[Category: Bacillus globigii migula 1900]]
	[[Category: Cellulase]]		[[Category: Cellulase]]
	[[Category: Meza, A N]]		[[Category: Meza, A N]]

OCA at 17:32, 19 January 2015

2015-01-19T17:32:54Z

← Older revision		Revision as of 17:32, 19 January 2015
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2l8a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L8A FirstGlance]. <br>		<table><tr><td colspan='2'>[[2l8a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L8A FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pzu\|3pzu]], [[3pzv\|3pzv]], [[3pzt\|3pzt]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pzu\|3pzu]], [[3pzv\|3pzv]], [[3pzt\|3pzt]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eglS, bglC, gld, BSU18130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eglS, bglC, gld, BSU18130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l8a RCSB], [http://www.ebi.ac.uk/pdbsum/2l8a PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l8a RCSB], [http://www.ebi.ac.uk/pdbsum/2l8a PDBsum]</span></td></tr>
	<table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 25:
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
	[[Category: Cellulase]]		[[Category: Cellulase]]
	[[Category: Meza, A N.]]		[[Category: Meza, A N]]
	[[Category: Murakami, M T.]]		[[Category: Murakami, M T]]
	[[Category: Navarro, R Z.]]		[[Category: Navarro, R Z]]
	[[Category: Neves, J L.]]		[[Category: Neves, J L]]
	[[Category: Paiva, J H.]]		[[Category: Paiva, J H]]
	[[Category: Santos, C R.]]		[[Category: Santos, C R]]
	[[Category: Sforca, M L.]]		[[Category: Sforca, M L]]
	[[Category: Zeri, A C.]]		[[Category: Zeri, A C]]
	[[Category: Carbohydrate-binding module]]		[[Category: Carbohydrate-binding module]]
	[[Category: Family 3]]		[[Category: Family 3]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 01:01, 2 October 2014

2014-10-02T01:01:49Z

← Older revision		Revision as of 01:01, 2 October 2014
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	[[~~Image~~:~~2l8a~~.~~png~~\|~~left~~\|~~200px~~]]		==Structure of a novel CBM3 lacking the calcium-binding site==
			<StructureSection load='2l8a' size='340' side='right' caption='[[2l8a]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2l8a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L8A FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pzu\|3pzu]], [[3pzv\|3pzv]], [[3pzt\|3pzt]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eglS, bglC, gld, BSU18130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l8a RCSB], [http://www.ebi.ac.uk/pdbsum/2l8a PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Cellulases participate in a number of biological events such as plant cell wall remodeling, nematode parasitism and microbial carbon uptake. Their ability to depolymerize crystalline cellulose is of great biotechnological interest for environmentally-compatible production of fuels from lignocellulosic biomass. However, industrial use of cellulases is somewhat limited both by their low catalytic efficiency and stability. In this work, we conducted a detailed functional and structural characterization of the thermostable cellulase 5A from Bacillus subtilis (BsCel5A), which consists of a GH5 catalytic domain fused to a family 3 carbohydrate-binding module (CBM3). NMR structural analysis revealed that the Bacillus CBM3 represents a new subfamily, which lacks the classical calcium-binding motif and variations in NMR frequencies in the presence of cellopentaose showed the importance of polar residues in the carbohydrate interaction. Together with the catalytic domain, the CBM3 forms a large planar surface for cellulose recognition, which conducts the substrate in a proper conformation to the active site and increases enzymatic efficiency. Notably, the manganese ion demonstrated to have a hyper-stabilizing effect on BsCel5A and by using deletion constructs and X-ray crystallography, we determined that this effect maps to a negatively charged motif located at the opposite face of the catalytic site.

	~~{{STRUCTURE_2l8a\| PDB=2l8a \| SCENE= }}~~		Dissecting structure-function-stability relationships of a thermostable GH5-CBM3 cellulase from Bacillus subtilis 168.,Santos C, Paiva J, Sforca M, Neves J, Navarro R, Cota J, Akao P, Hoffmam ZB, Meza A, Smetana J, Nogueira M, Polikarpov I, Xavier-Neto J, Squina F, Ward RJ, Ruller R, Zeri A, Murakami MT Biochem J. 2011 Sep 1. PMID:21880019<ref>PMID:21880019</ref>

	~~===Structure~~ of ~~a novel CBM3 lacking~~ the ~~calcium-binding site===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_21880019}}~~

	~~==About this Structure==~~
	~~[[2l8a]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_subtilis Bacillus subtilis]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2L8A OCA].~~

	==See Also==		==See Also==
	*[[Glucanase\|Glucanase]]		*[[Glucanase\|Glucanase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:021880019<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
	[[Category: Cellulase]]		[[Category: Cellulase]]

OCA at 15:49, 20 October 2012

2012-10-20T15:49:08Z

← Older revision		Revision as of 15:49, 20 October 2012
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	==About this Structure==		==About this Structure==
	[[2l8a]] is a 1 chain structure ~~of [[Glucanase]]~~ with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8A OCA].		[[2l8a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8A OCA].

	==See Also==		==See Also==

OCA at 19:36, 26 July 2012

2012-07-26T19:36:19Z

← Older revision		Revision as of 19:36, 26 July 2012
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	[[Image:2l8a.png\|left\|200px]]		[[Image:2l8a.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2l8a", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2l8a\| PDB=2l8a \| SCENE= }}		{{STRUCTURE_2l8a\| PDB=2l8a \| SCENE= }}

	===Structure of a novel CBM3 lacking the calcium-binding site===		===Structure of a novel CBM3 lacking the calcium-binding site===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_21880019}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 21880019 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_21880019}}		{{ABSTRACT_PUBMED_21880019}}

	==About this Structure==		==About this Structure==
	[[2l8a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8A OCA].		[[2l8a]] is a 1 chain structure of [[Glucanase]] with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8A OCA].

			==See Also==
			*[[Glucanase\|Glucanase]]

	==Reference==		==Reference==

OCA at 06:10, 28 December 2011

2011-12-28T06:10:00Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:2l8a.png|left|200px]]
-The entry 2l8a is ON HOLD  until Paper Publication
+<!--
-Authors: Paiva, J.H., Meza, A.N., Sforca, M.L., Navarro, R.Z., Neves, J.L., Santos, C.R., Murakami, M.T., Zeri, A.C.
+===Structure of a novel CBM3 lacking the calcium-binding site===
-Description: Structure of a novel CBM3 lacking the calcium-binding site