2ldf - Revision history

OCA at 06:10, 27 November 2024

2024-11-27T06:10:35Z

← Older revision		Revision as of 06:10, 27 November 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ldf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atractaspis_microlepidota_microlepidota Atractaspis microlepidota microlepidota]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LDF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LDF FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ldf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atractaspis_microlepidota_microlepidota Atractaspis microlepidota microlepidota]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LDF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LDF FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ldf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ldf OCA], [https://pdbe.org/2ldf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ldf RCSB], [https://www.ebi.ac.uk/pdbsum/2ldf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ldf ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ldf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ldf OCA], [https://pdbe.org/2ldf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ldf RCSB], [https://www.ebi.ac.uk/pdbsum/2ldf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ldf ProSAT]</span></td></tr>
	</table>		</table>
	~~== Function ==~~
	[https://www.uniprot.org/uniprot/SRTXL_ATRMM SRTXL_ATRMM] Vasoconstrictor activity. These toxins cause cardiac arrest probably as a result of coronary vasospasm. The major effects of sarafotoxin-m are a progressive decrease in heart rate (bradycardia) that turns into an arrhythmic phase that is followed by an A-V block.<ref>PMID:15350691</ref> Sarafotoxin-m: vasoconstrictor activity. Causes cardiac arrest probably as a result of coronary vasospasm (By similarity). Displays low agonistic activities towards endothelin-2 receptor (EDNRB) (displays affinity in the micromolar range) (PubMed:21889567).<ref>PMID:15350691</ref> <ref>PMID:21889567</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 08:56, 14 June 2023

2023-06-14T08:56:19Z

← Older revision		Revision as of 08:56, 14 June 2023
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	==Solution structure of the long sarafotoxin srtx-m==		==Solution structure of the long sarafotoxin srtx-m==
	<StructureSection load='2ldf' size='340' side='right'caption='[[2ldf~~]], [[NMR_Ensembles_of_Models \| 10 NMR models~~]]' scene=''>		<StructureSection load='2ldf' size='340' side='right'caption='[[2ldf]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ldf]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LDF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LDF FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ldf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atractaspis_microlepidota_microlepidota Atractaspis microlepidota microlepidota]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LDF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LDF FirstGlance]. <br>
	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2lde\|2lde]]</div></td></tr>~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ldf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ldf OCA], [https://pdbe.org/2ldf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ldf RCSB], [https://www.ebi.ac.uk/pdbsum/2ldf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ldf ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ldf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ldf OCA], [https://pdbe.org/2ldf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ldf RCSB], [https://www.ebi.ac.uk/pdbsum/2ldf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ldf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/SRTXL_ATRMM SRTXL_ATRMM]] Vasoconstrictor activity. These toxins cause cardiac arrest probably as a result of coronary vasospasm. The major effects of sarafotoxin-m are a progressive decrease in heart rate (bradycardia) that turns into an arrhythmic phase that is followed by an A-V block.<ref>PMID:15350691</ref> Sarafotoxin-m: vasoconstrictor activity. Causes cardiac arrest probably as a result of coronary vasospasm (By similarity). Displays low agonistic activities towards endothelin-2 receptor (EDNRB) (displays affinity in the micromolar range) (PubMed:21889567).<ref>PMID:15350691</ref> <ref>PMID:21889567</ref>		[https://www.uniprot.org/uniprot/SRTXL_ATRMM SRTXL_ATRMM] Vasoconstrictor activity. These toxins cause cardiac arrest probably as a result of coronary vasospasm. The major effects of sarafotoxin-m are a progressive decrease in heart rate (bradycardia) that turns into an arrhythmic phase that is followed by an A-V block.<ref>PMID:15350691</ref> Sarafotoxin-m: vasoconstrictor activity. Causes cardiac arrest probably as a result of coronary vasospasm (By similarity). Displays low agonistic activities towards endothelin-2 receptor (EDNRB) (displays affinity in the micromolar range) (PubMed:21889567).<ref>PMID:15350691</ref> <ref>PMID:21889567</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Atractaspis microlepidota microlepidota]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Cordier, F]]		[[Category: Cordier F]]
	[[Category: Delepierre, M]]		[[Category: Delepierre M]]
	[[Category: Ducancel, F]]		[[Category: Ducancel F]]
	[[Category: Hajj, M]]		[[Category: Hajj M]]
	[[Category: Servent, D]]		[[Category: Servent D]]
	[[Category: Zorba, A]]		[[Category: Zorba A]]
	~~[[Category: Endothelin-like peptide]]~~
	~~[[Category: Toxin~~]]

OCA at 07:50, 9 February 2022

2022-02-09T07:50:52Z

← Older revision		Revision as of 07:50, 9 February 2022
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	<StructureSection load='2ldf' size='340' side='right'caption='[[2ldf]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='2ldf' size='340' side='right'caption='[[2ldf]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ldf]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LDF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2LDF FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ldf]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LDF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LDF FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2lde\|2lde]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2lde\|2lde]]</div></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2ldf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ldf OCA], [~~http~~://pdbe.org/2ldf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ldf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ldf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ldf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ldf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ldf OCA], [https://pdbe.org/2ldf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ldf RCSB], [https://www.ebi.ac.uk/pdbsum/2ldf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ldf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/SRTXL_ATRMM SRTXL_ATRMM]] Vasoconstrictor activity. These toxins cause cardiac arrest probably as a result of coronary vasospasm. The major effects of sarafotoxin-m are a progressive decrease in heart rate (bradycardia) that turns into an arrhythmic phase that is followed by an A-V block.<ref>PMID:15350691</ref> Sarafotoxin-m: vasoconstrictor activity. Causes cardiac arrest probably as a result of coronary vasospasm (By similarity). Displays low agonistic activities towards endothelin-2 receptor (EDNRB) (displays affinity in the micromolar range) (PubMed:21889567).<ref>PMID:15350691</ref> <ref>PMID:21889567</ref>		[[https://www.uniprot.org/uniprot/SRTXL_ATRMM SRTXL_ATRMM]] Vasoconstrictor activity. These toxins cause cardiac arrest probably as a result of coronary vasospasm. The major effects of sarafotoxin-m are a progressive decrease in heart rate (bradycardia) that turns into an arrhythmic phase that is followed by an A-V block.<ref>PMID:15350691</ref> Sarafotoxin-m: vasoconstrictor activity. Causes cardiac arrest probably as a result of coronary vasospasm (By similarity). Displays low agonistic activities towards endothelin-2 receptor (EDNRB) (displays affinity in the micromolar range) (PubMed:21889567).<ref>PMID:15350691</ref> <ref>PMID:21889567</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:02, 6 May 2020

2020-05-06T07:02:52Z

← Older revision		Revision as of 07:02, 6 May 2020
Line 1:		Line 1:

	==Solution structure of the long sarafotoxin srtx-m==		==Solution structure of the long sarafotoxin srtx-m==
	<StructureSection load='2ldf' size='340' side='right' caption='[[2ldf]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='2ldf' size='340' side='right'caption='[[2ldf]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ldf]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LDF OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2LDF FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ldf]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LDF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2LDF FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2lde\|2lde]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2lde\|2lde]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ldf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ldf OCA], [http://pdbe.org/2ldf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ldf RCSB], [http://www.ebi.ac.uk/pdbsum/2ldf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ldf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2ldf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ldf OCA], [http://pdbe.org/2ldf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ldf RCSB], [http://www.ebi.ac.uk/pdbsum/2ldf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ldf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Cordier, F]]		[[Category: Cordier, F]]
	[[Category: Delepierre, M]]		[[Category: Delepierre, M]]

OCA at 19:58, 1 August 2018

2018-08-01T19:58:49Z

← Older revision		Revision as of 19:58, 1 August 2018
Line 1:		Line 1:

	==Solution structure of the long sarafotoxin srtx-m==		==Solution structure of the long sarafotoxin srtx-m==
	<StructureSection load='2ldf' size='340' side='right' caption='[[2ldf]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='2ldf' size='340' side='right' caption='[[2ldf]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
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	<table><tr><td colspan='2'>[[2ldf]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LDF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LDF FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ldf]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LDF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LDF FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2lde\|2lde]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2lde\|2lde]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ldf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ldf OCA], [http://pdbe.org/2ldf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ldf RCSB], [http://www.ebi.ac.uk/pdbsum/2ldf PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ldf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ldf OCA], [http://pdbe.org/2ldf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ldf RCSB], [http://www.ebi.ac.uk/pdbsum/2ldf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ldf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 03:31, 11 September 2015

2015-09-11T03:31:10Z

← Older revision		Revision as of 03:31, 11 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[2ldf]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LDF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LDF FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ldf]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LDF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LDF FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2lde\|2lde]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2lde\|2lde]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ldf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ldf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ldf RCSB], [http://www.ebi.ac.uk/pdbsum/2ldf PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ldf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ldf OCA], [http://pdbe.org/2ldf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ldf RCSB], [http://www.ebi.ac.uk/pdbsum/2ldf PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/~~SRTDL_ATRMM SRTDL_ATRMM~~]] Vasoconstrictor activity. These toxins cause cardiac arrest probably as a result of coronary vasospasm. The major effects of sarafotoxin-m are a progressive decrease in heart rate (bradycardia) that turns into an arrhythmic phase that is followed by an A-V block.<ref>PMID:15350691</ref> Sarafotoxin-m: vasoconstrictor activity. Causes cardiac arrest probably as a result of coronary vasospasm (By similarity). Displays low agonistic activities towards endothelin-2 receptor (EDNRB) (displays affinity in the micromolar range) (PubMed:21889567).<ref>PMID:15350691</ref>		[[http://www.uniprot.org/uniprot/SRTXL_ATRMM SRTXL_ATRMM]] Vasoconstrictor activity. These toxins cause cardiac arrest probably as a result of coronary vasospasm. The major effects of sarafotoxin-m are a progressive decrease in heart rate (bradycardia) that turns into an arrhythmic phase that is followed by an A-V block.<ref>PMID:15350691</ref> Sarafotoxin-m: vasoconstrictor activity. Causes cardiac arrest probably as a result of coronary vasospasm (By similarity). Displays low agonistic activities towards endothelin-2 receptor (EDNRB) (displays affinity in the micromolar range) (PubMed:21889567).<ref>PMID:15350691</ref> <ref>PMID:21889567</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2ldf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:30, 25 December 2014

2014-12-25T10:30:48Z

← Older revision		Revision as of 10:30, 25 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ldf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ldf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ldf RCSB], [http://www.ebi.ac.uk/pdbsum/2ldf PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ldf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ldf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ldf RCSB], [http://www.ebi.ac.uk/pdbsum/2ldf PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/SRTDL_ATRMM SRTDL_ATRMM]] Vasoconstrictor activity. These toxins cause cardiac arrest probably as a result of coronary vasospasm. The major effects of sarafotoxin-m are a progressive decrease in heart rate (bradycardia) that turns into an arrhythmic phase that is followed by an A-V block.<ref>PMID:15350691</ref> Sarafotoxin-m: vasoconstrictor activity. Causes cardiac arrest probably as a result of coronary vasospasm (By similarity). Displays low agonistic activities towards endothelin-2 receptor (EDNRB) (displays affinity in the micromolar range) (PubMed:21889567).<ref>PMID:15350691</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 05:56, 22 December 2014

2014-12-22T05:56:32Z

← Older revision		Revision as of 05:56, 22 December 2014
Line 2:		Line 2:
	<StructureSection load='2ldf' size='340' side='right' caption='[[2ldf]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='2ldf' size='340' side='right' caption='[[2ldf]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ldf]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LDF OCA]. <br>		<table><tr><td colspan='2'>[[2ldf]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LDF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LDF FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2lde\|2lde]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2lde\|2lde]]</td></tr>
	<tr~~><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class~~='~~plainlinks~~'~~>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>~~		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ldf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ldf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ldf RCSB], [http://www.ebi.ac.uk/pdbsum/2ldf PDBsum]</span></td></tr>
	~~<tr~~><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ldf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ldf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ldf RCSB], [http://www.ebi.ac.uk/pdbsum/2ldf PDBsum]</span></td></tr>		</table>
	<table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 12:
	Pharmacological and structural characterization of long-sarafotoxins, a new family of endothelin-like peptides: Role of the C-terminus extension.,Mourier G, Hajj M, Cordier F, Zorba A, Gao X, Coskun T, Herbet A, Marcon E, Beau F, Delepierre M, Ducancel F, Servent D Biochimie. 2011 Aug 29. PMID:21889567<ref>PMID:21889567</ref>		Pharmacological and structural characterization of long-sarafotoxins, a new family of endothelin-like peptides: Role of the C-terminus extension.,Mourier G, Hajj M, Cordier F, Zorba A, Gao X, Coskun T, Herbet A, Marcon E, Beau F, Delepierre M, Ducancel F, Servent D Biochimie. 2011 Aug 29. PMID:21889567<ref>PMID:21889567</ref>

	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 19:		Line 18:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Cordier, F.]]		[[Category: Cordier, F]]
	[[Category: Delepierre, M.]]		[[Category: Delepierre, M]]
	[[Category: Ducancel, F.]]		[[Category: Ducancel, F]]
	[[Category: Hajj, M.]]		[[Category: Hajj, M]]
	[[Category: Servent, D.]]		[[Category: Servent, D]]
	[[Category: Zorba, A.]]		[[Category: Zorba, A]]
	[[Category: Endothelin-like peptide]]		[[Category: Endothelin-like peptide]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 09:51, 1 May 2014

2014-05-01T09:51:19Z

← Older revision		Revision as of 09:51, 1 May 2014
Line 2:		Line 2:
	<StructureSection load='2ldf' size='340' side='right' caption='[[2ldf]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='2ldf' size='340' side='right' caption='[[2ldf]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	[[2ldf]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LDF OCA]. <br>		<table><tr><td colspan='2'>[[2ldf]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LDF OCA]. <br>
	<b>[[Related_structure\|Related:]]</b> [[2lde\|2lde]]<br>		</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2lde\|2lde]]</td></tr>
	<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>		<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
	<b>Resources:</b> <span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ldf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ldf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ldf RCSB], [http://www.ebi.ac.uk/pdbsum/2ldf PDBsum]</span><br>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ldf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ldf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ldf RCSB], [http://www.ebi.ac.uk/pdbsum/2ldf PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
	Long-sarafotoxins (l-SRTXs) have recently been identified in both the venom of Atractaspis microlepidota and that of Atractaspis irregularis. They are characterized by different C-terminus extensions that follow the invariant Trp21, which plays a crucial role in endothelin-receptor binding. We initially determined the toxicity and three-dimensional structures of two chemically synthesized l-SRTXs that have different C-terminus extensions, namely SRTX-m (24 aa, including extension "D-E-P") and SRTX-i3 (25 aa, including extension "V-N-R-N"). Both peptides were shown to be highly toxic in mice and displayed the cysteine-stabilized alpha-helical motif that characterizes endothelins and short-SRTXs, to which a longer C-terminus with variable flexibility is added. To discern the functional and pharmacological consequences of the supplementary amino acids, different chimerical as well as truncated forms of SRTX were designed and synthesized. Thus, we either removed the extra-C-terminal residues of SRTX-m or i3, or grafted the latter onto the C-terminal extremity of a short-SRTX (s-SRTX) (ie. SRTX-b). Our competitive binding assays where SRTXs competed for iodinated endothelin-1 binding to cloned ET(A) and ET(B) receptor subtypes over-expressed in CHO cells, revealed the essential role of the C-terminus extensions for ET-receptor recognition. Indeed, l-SRTXs displayed an affinity three to four orders of magnitude lower as compared to SRTX-b for the two receptor subtypes. Moreover, grafting the C-terminus extension to SRTX-b induced a drastic decrease in affinity, while its removal (truncated l-SRTXs) yielded an affinity for ET-receptors similar to that of s-SRTXs. Furthermore, we established by intracellular Ca(2+) measurements that l-SRTXs, as well as s-SRTXs, display agonistic activities. We thus confirmed in these functional assays the major difference in potency for these two SRTX families as well as the crucial role of the C-terminus extension in their various pharmacological profiles. Finally, one of the chimeric toxin synthesized in this study appears to be one of the most potent and selective ligand of the ET(B) receptor known to date.		Long-sarafotoxins (l-SRTXs) have recently been identified in both the venom of Atractaspis microlepidota and that of Atractaspis irregularis. They are characterized by different C-terminus extensions that follow the invariant Trp21, which plays a crucial role in endothelin-receptor binding. We initially determined the toxicity and three-dimensional structures of two chemically synthesized l-SRTXs that have different C-terminus extensions, namely SRTX-m (24 aa, including extension "D-E-P") and SRTX-i3 (25 aa, including extension "V-N-R-N"). Both peptides were shown to be highly toxic in mice and displayed the cysteine-stabilized alpha-helical motif that characterizes endothelins and short-SRTXs, to which a longer C-terminus with variable flexibility is added. To discern the functional and pharmacological consequences of the supplementary amino acids, different chimerical as well as truncated forms of SRTX were designed and synthesized. Thus, we either removed the extra-C-terminal residues of SRTX-m or i3, or grafted the latter onto the C-terminal extremity of a short-SRTX (s-SRTX) (ie. SRTX-b). Our competitive binding assays where SRTXs competed for iodinated endothelin-1 binding to cloned ET(A) and ET(B) receptor subtypes over-expressed in CHO cells, revealed the essential role of the C-terminus extensions for ET-receptor recognition. Indeed, l-SRTXs displayed an affinity three to four orders of magnitude lower as compared to SRTX-b for the two receptor subtypes. Moreover, grafting the C-terminus extension to SRTX-b induced a drastic decrease in affinity, while its removal (truncated l-SRTXs) yielded an affinity for ET-receptors similar to that of s-SRTXs. Furthermore, we established by intracellular Ca(2+) measurements that l-SRTXs, as well as s-SRTXs, display agonistic activities. We thus confirmed in these functional assays the major difference in potency for these two SRTX families as well as the crucial role of the C-terminus extension in their various pharmacological profiles. Finally, one of the chimeric toxin synthesized in this study appears to be one of the most potent and selective ligand of the ET(B) receptor known to date.
Line 12:		Line 14:

	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>		From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
			</div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:14, 30 April 2014

2014-04-30T10:14:41Z

← Older revision		Revision as of 10:14, 30 April 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	[[2ldf]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LDF OCA]. <br>		[[2ldf]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LDF OCA]. <br>
	<b>Related:</b> [[2lde\|2lde]]<br>		<b>[[Related_structure\|Related:]]</b> [[2lde\|2lde]]<br>
	<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>		<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
			<b>Resources:</b> <span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ldf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ldf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ldf RCSB], [http://www.ebi.ac.uk/pdbsum/2ldf PDBsum]</span><br>
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
	Long-sarafotoxins (l-SRTXs) have recently been identified in both the venom of Atractaspis microlepidota and that of Atractaspis irregularis. They are characterized by different C-terminus extensions that follow the invariant Trp21, which plays a crucial role in endothelin-receptor binding. We initially determined the toxicity and three-dimensional structures of two chemically synthesized l-SRTXs that have different C-terminus extensions, namely SRTX-m (24 aa, including extension "D-E-P") and SRTX-i3 (25 aa, including extension "V-N-R-N"). Both peptides were shown to be highly toxic in mice and displayed the cysteine-stabilized alpha-helical motif that characterizes endothelins and short-SRTXs, to which a longer C-terminus with variable flexibility is added. To discern the functional and pharmacological consequences of the supplementary amino acids, different chimerical as well as truncated forms of SRTX were designed and synthesized. Thus, we either removed the extra-C-terminal residues of SRTX-m or i3, or grafted the latter onto the C-terminal extremity of a short-SRTX (s-SRTX) (ie. SRTX-b). Our competitive binding assays where SRTXs competed for iodinated endothelin-1 binding to cloned ET(A) and ET(B) receptor subtypes over-expressed in CHO cells, revealed the essential role of the C-terminus extensions for ET-receptor recognition. Indeed, l-SRTXs displayed an affinity three to four orders of magnitude lower as compared to SRTX-b for the two receptor subtypes. Moreover, grafting the C-terminus extension to SRTX-b induced a drastic decrease in affinity, while its removal (truncated l-SRTXs) yielded an affinity for ET-receptors similar to that of s-SRTXs. Furthermore, we established by intracellular Ca(2+) measurements that l-SRTXs, as well as s-SRTXs, display agonistic activities. We thus confirmed in these functional assays the major difference in potency for these two SRTX families as well as the crucial role of the C-terminus extension in their various pharmacological profiles. Finally, one of the chimeric toxin synthesized in this study appears to be one of the most potent and selective ligand of the ET(B) receptor known to date.		Long-sarafotoxins (l-SRTXs) have recently been identified in both the venom of Atractaspis microlepidota and that of Atractaspis irregularis. They are characterized by different C-terminus extensions that follow the invariant Trp21, which plays a crucial role in endothelin-receptor binding. We initially determined the toxicity and three-dimensional structures of two chemically synthesized l-SRTXs that have different C-terminus extensions, namely SRTX-m (24 aa, including extension "D-E-P") and SRTX-i3 (25 aa, including extension "V-N-R-N"). Both peptides were shown to be highly toxic in mice and displayed the cysteine-stabilized alpha-helical motif that characterizes endothelins and short-SRTXs, to which a longer C-terminus with variable flexibility is added. To discern the functional and pharmacological consequences of the supplementary amino acids, different chimerical as well as truncated forms of SRTX were designed and synthesized. Thus, we either removed the extra-C-terminal residues of SRTX-m or i3, or grafted the latter onto the C-terminal extremity of a short-SRTX (s-SRTX) (ie. SRTX-b). Our competitive binding assays where SRTXs competed for iodinated endothelin-1 binding to cloned ET(A) and ET(B) receptor subtypes over-expressed in CHO cells, revealed the essential role of the C-terminus extensions for ET-receptor recognition. Indeed, l-SRTXs displayed an affinity three to four orders of magnitude lower as compared to SRTX-b for the two receptor subtypes. Moreover, grafting the C-terminus extension to SRTX-b induced a drastic decrease in affinity, while its removal (truncated l-SRTXs) yielded an affinity for ET-receptors similar to that of s-SRTXs. Furthermore, we established by intracellular Ca(2+) measurements that l-SRTXs, as well as s-SRTXs, display agonistic activities. We thus confirmed in these functional assays the major difference in potency for these two SRTX families as well as the crucial role of the C-terminus extension in their various pharmacological profiles. Finally, one of the chimeric toxin synthesized in this study appears to be one of the most potent and selective ligand of the ET(B) receptor known to date.