2lt7 - Revision history

OCA at 06:57, 1 May 2024

2024-05-01T06:57:12Z

← Older revision		Revision as of 06:57, 1 May 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2lt7]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LT7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LT7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2lt7]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LT7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LT7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lt7 OCA], [https://pdbe.org/2lt7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lt7 RCSB], [https://www.ebi.ac.uk/pdbsum/2lt7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lt7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lt7 OCA], [https://pdbe.org/2lt7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lt7 RCSB], [https://www.ebi.ac.uk/pdbsum/2lt7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lt7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/KAISO_HUMAN KAISO_HUMAN] Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2.<ref>PMID:11445535</ref> <ref>PMID:14527417</ref> <ref>PMID:15548582</ref> <ref>PMID:15817151</ref>		[https://www.uniprot.org/uniprot/KAISO_HUMAN KAISO_HUMAN] Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2.<ref>PMID:11445535</ref> <ref>PMID:14527417</ref> <ref>PMID:15548582</ref> <ref>PMID:15817151</ref>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Methylation of CpG dinucleotides in DNA is a common epigenetic modification in eukaryotes that plays a central role in maintenance of genome stability, gene silencing, genomic imprinting, development, and disease. Kaiso, a bifunctional Cys(2)His(2) zinc finger protein implicated in tumor-cell proliferation, binds to both methylated CpG (mCpG) sites and a specific nonmethylated DNA motif (TCCTGCNA) and represses transcription by recruiting chromatin remodeling corepression machinery to target genes. Here we report structures of the Kaiso zinc finger DNA-binding domain in complex with its nonmethylated, sequence-specific DNA target (KBS) and with a symmetrically methylated DNA sequence derived from the promoter region of E-cadherin. Recognition of specific bases in the major groove of the core KBS and mCpG sites is accomplished through both classical and methyl CH...O hydrogen-bonding interactions with residues in the first two zinc fingers, whereas residues in the C-terminal extension following the third zinc finger bind in the opposing minor groove and are required for high-affinity binding. The C-terminal region is disordered in the free protein and adopts an ordered structure upon binding to DNA. The structures of these Kaiso complexes provide insights into the mechanism by which a zinc finger protein can recognize mCpG sites as well as a specific, nonmethylated regulatory DNA sequence.

	Molecular basis for recognition of methylated and specific DNA sequences by the zinc finger protein Kaiso.,Buck-Koehntop BA, Stanfield RL, Ekiert DC, Martinez-Yamout MA, Dyson HJ, Wilson IA, Wright PE Proc Natl Acad Sci U S A. 2012 Sep 4. PMID:22949637<ref>PMID:22949637</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2lt7" style="background-color:#fffaf0;"></div>~~
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:08, 22 February 2023

2023-02-22T13:08:52Z

← Older revision		Revision as of 13:08, 22 February 2023
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	==Solution NMR structure of Kaiso zinc finger DNA binding domain in complex with Kaiso binding site DNA==		==Solution NMR structure of Kaiso zinc finger DNA binding domain in complex with Kaiso binding site DNA==
	<StructureSection load='2lt7' size='340' side='right'caption='[[2lt7~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>		<StructureSection load='2lt7' size='340' side='right'caption='[[2lt7]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2lt7]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LT7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LT7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2lt7]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LT7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LT7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ZBTB33, KAISO, ZNF348 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lt7 OCA], [https://pdbe.org/2lt7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lt7 RCSB], [https://www.ebi.ac.uk/pdbsum/2lt7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lt7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lt7 OCA], [https://pdbe.org/2lt7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lt7 RCSB], [https://www.ebi.ac.uk/pdbsum/2lt7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lt7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/KAISO_HUMAN KAISO_HUMAN]] Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2.<ref>PMID:11445535</ref> <ref>PMID:14527417</ref> <ref>PMID:15548582</ref> <ref>PMID:15817151</ref>		[https://www.uniprot.org/uniprot/KAISO_HUMAN KAISO_HUMAN] Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2.<ref>PMID:11445535</ref> <ref>PMID:14527417</ref> <ref>PMID:15548582</ref> <ref>PMID:15817151</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Human~~]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Buck-Koehntop~~, B A~~]]		[[Category: Buck-Koehntop BA]]
	[[Category: Dyson, H]]		[[Category: Dyson H]]
	[[Category: Ekiert~~, D C~~]]		[[Category: Ekiert DC]]
	[[Category: Martinez-Yamout~~, M A~~]]		[[Category: Martinez-Yamout MA]]
	[[Category: Stanfield~~, R L~~]]		[[Category: Stanfield RL]]
	[[Category: Wilson~~, I A~~]]		[[Category: Wilson IA]]
	[[Category: Wright~~, P E]]~~		[[Category: Wright PE]]
	~~[[Category: Double helix]]~~
	~~[[Category: Metal binding protein-dna complex]]~~
	~~[[Category: Zinc finger~~]]

OCA at 10:52, 19 May 2021

2021-05-19T10:52:40Z

← Older revision		Revision as of 10:52, 19 May 2021
Line 1:		Line 1:

	==Solution NMR structure of Kaiso zinc finger DNA binding domain in complex with Kaiso binding site DNA==		==Solution NMR structure of Kaiso zinc finger DNA binding domain in complex with Kaiso binding site DNA==
	<StructureSection load='2lt7' size='340' side='right' caption='[[2lt7]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2lt7' size='340' side='right'caption='[[2lt7]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2lt7]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LT7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2LT7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2lt7]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LT7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LT7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ZBTB33, KAISO, ZNF348 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ZBTB33, KAISO, ZNF348 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2lt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lt7 OCA], [~~http~~://pdbe.org/2lt7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2lt7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2lt7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2lt7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lt7 OCA], [https://pdbe.org/2lt7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lt7 RCSB], [https://www.ebi.ac.uk/pdbsum/2lt7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lt7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/KAISO_HUMAN KAISO_HUMAN]] Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2.<ref>PMID:11445535</ref> <ref>PMID:14527417</ref> <ref>PMID:15548582</ref> <ref>PMID:15817151</ref>		[[https://www.uniprot.org/uniprot/KAISO_HUMAN KAISO_HUMAN]] Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2.<ref>PMID:11445535</ref> <ref>PMID:14527417</ref> <ref>PMID:15548582</ref> <ref>PMID:15817151</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
			[[Category: Large Structures]]
	[[Category: Buck-Koehntop, B A]]		[[Category: Buck-Koehntop, B A]]
	[[Category: Dyson, H]]		[[Category: Dyson, H]]

OCA at 19:36, 1 August 2018

2018-08-01T19:36:25Z

← Older revision		Revision as of 19:36, 1 August 2018
Line 1:		Line 1:

	==Solution NMR structure of Kaiso zinc finger DNA binding domain in complex with Kaiso binding site DNA==		==Solution NMR structure of Kaiso zinc finger DNA binding domain in complex with Kaiso binding site DNA==
	<StructureSection load='2lt7' size='340' side='right' caption='[[2lt7]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2lt7' size='340' side='right' caption='[[2lt7]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ZBTB33, KAISO, ZNF348 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ZBTB33, KAISO, ZNF348 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lt7 OCA], [http://pdbe.org/2lt7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lt7 RCSB], [http://www.ebi.ac.uk/pdbsum/2lt7 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lt7 OCA], [http://pdbe.org/2lt7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lt7 RCSB], [http://www.ebi.ac.uk/pdbsum/2lt7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2lt7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 16:45, 11 September 2015

2015-09-11T16:45:36Z

← Older revision		Revision as of 16:45, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2lt7' size='340' side='right' caption='[[2lt7]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2lt7' size='340' side='right' caption='[[2lt7]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2lt7]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LT7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LT7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2lt7]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LT7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LT7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ZBTB33, KAISO, ZNF348 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ZBTB33, KAISO, ZNF348 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lt7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lt7 RCSB], [http://www.ebi.ac.uk/pdbsum/2lt7 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lt7 OCA], [http://pdbe.org/2lt7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lt7 RCSB], [http://www.ebi.ac.uk/pdbsum/2lt7 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2lt7" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Homo sapiens~~]]		[[Category: Human]]
	[[Category: Buck-Koehntop, B A]]		[[Category: Buck-Koehntop, B A]]
	[[Category: Dyson, H]]		[[Category: Dyson, H]]

OCA at 19:11, 25 December 2014

2014-12-25T19:11:39Z

← Older revision		Revision as of 19:11, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lt7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lt7 RCSB], [http://www.ebi.ac.uk/pdbsum/2lt7 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lt7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lt7 RCSB], [http://www.ebi.ac.uk/pdbsum/2lt7 PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/KAISO_HUMAN KAISO_HUMAN]] Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2.<ref>PMID:11445535</ref> <ref>PMID:14527417</ref> <ref>PMID:15548582</ref> <ref>PMID:15817151</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 05:17, 22 December 2014

2014-12-22T05:17:44Z

← Older revision		Revision as of 05:17, 22 December 2014
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2lt7]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LT7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LT7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2lt7]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LT7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LT7 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ZBTB33, KAISO, ZNF348 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ZBTB33, KAISO, ZNF348 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lt7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lt7 RCSB], [http://www.ebi.ac.uk/pdbsum/2lt7 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lt7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lt7 RCSB], [http://www.ebi.ac.uk/pdbsum/2lt7 PDBsum]</span></td></tr>
	<table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Buck-Koehntop, B A.]]		[[Category: Buck-Koehntop, B A]]
	[[Category: Dyson, H.]]		[[Category: Dyson, H]]
	[[Category: Ekiert, D C.]]		[[Category: Ekiert, D C]]
	[[Category: Martinez-Yamout, M A.]]		[[Category: Martinez-Yamout, M A]]
	[[Category: Stanfield, R L.]]		[[Category: Stanfield, R L]]
	[[Category: Wilson, I A.]]		[[Category: Wilson, I A]]
	[[Category: Wright, P E.]]		[[Category: Wright, P E]]
	[[Category: Double helix]]		[[Category: Double helix]]
	[[Category: Metal binding protein-dna complex]]		[[Category: Metal binding protein-dna complex]]
	[[Category: Zinc finger]]		[[Category: Zinc finger]]

OCA at 06:52, 25 June 2014

2014-06-25T06:52:06Z

← Older revision		Revision as of 06:52, 25 June 2014
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	[[~~Image~~:~~2lt7~~.~~png~~\|~~left~~\|~~200px~~]]		==Solution NMR structure of Kaiso zinc finger DNA binding domain in complex with Kaiso binding site DNA==
			<StructureSection load='2lt7' size='340' side='right' caption='[[2lt7]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2lt7]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LT7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LT7 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ZBTB33, KAISO, ZNF348 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lt7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lt7 RCSB], [http://www.ebi.ac.uk/pdbsum/2lt7 PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Methylation of CpG dinucleotides in DNA is a common epigenetic modification in eukaryotes that plays a central role in maintenance of genome stability, gene silencing, genomic imprinting, development, and disease. Kaiso, a bifunctional Cys(2)His(2) zinc finger protein implicated in tumor-cell proliferation, binds to both methylated CpG (mCpG) sites and a specific nonmethylated DNA motif (TCCTGCNA) and represses transcription by recruiting chromatin remodeling corepression machinery to target genes. Here we report structures of the Kaiso zinc finger DNA-binding domain in complex with its nonmethylated, sequence-specific DNA target (KBS) and with a symmetrically methylated DNA sequence derived from the promoter region of E-cadherin. Recognition of specific bases in the major groove of the core KBS and mCpG sites is accomplished through both classical and methyl CH...O hydrogen-bonding interactions with residues in the first two zinc fingers, whereas residues in the C-terminal extension following the third zinc finger bind in the opposing minor groove and are required for high-affinity binding. The C-terminal region is disordered in the free protein and adopts an ordered structure upon binding to DNA. The structures of these Kaiso complexes provide insights into the mechanism by which a zinc finger protein can recognize mCpG sites as well as a specific, nonmethylated regulatory DNA sequence.

	~~{{STRUCTURE_2lt7\| PDB=2lt7 \| SCENE= }}~~		Molecular basis for recognition of methylated and specific DNA sequences by the zinc finger protein Kaiso.,Buck-Koehntop BA, Stanfield RL, Ekiert DC, Martinez-Yamout MA, Dyson HJ, Wilson IA, Wright PE Proc Natl Acad Sci U S A. 2012 Sep 4. PMID:22949637<ref>PMID:22949637</ref>

	~~===Solution NMR structure~~ of ~~Kaiso zinc finger DNA binding domain in complex with Kaiso binding site DNA===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_22949637}}~~		== References ==
			<references/>
	==~~About this Structure~~==		__TOC__
	~~[[2lt7]] is a 3 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LT7 OCA].~~		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Buck-Koehntop, B A.]]		[[Category: Buck-Koehntop, B A.]]

OCA at 06:25, 19 September 2012

2012-09-19T06:25:47Z

← Older revision		Revision as of 06:25, 19 September 2012
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	[[Image:2lt7.~~jpg~~\|left\|200px]]		[[Image:2lt7.png\|left\|200px]]

	{{STRUCTURE_2lt7\| PDB=2lt7 \| SCENE= }}		{{STRUCTURE_2lt7\| PDB=2lt7 \| SCENE= }}
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	===Solution NMR structure of Kaiso zinc finger DNA binding domain in complex with Kaiso binding site DNA===		===Solution NMR structure of Kaiso zinc finger DNA binding domain in complex with Kaiso binding site DNA===

			{{ABSTRACT_PUBMED_22949637}}

	==About this Structure==		==About this Structure==

OCA at 05:29, 5 September 2012

2012-09-05T05:29:22Z

← Older revision		Revision as of 05:29, 5 September 2012
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	~~'''Unreleased structure'''~~		[[Image:2lt7.jpg\|left\|200px]]

	~~The entry~~ 2lt7 ~~is ON HOLD~~ ~~until Paper Publication~~		{{STRUCTURE_2lt7\| PDB=2lt7 \| SCENE= }}

	~~Authors: Buck-Koehntop, B.A., Stanfield, R.L., Ekiert, D.C., Martinez-Yamout, M.A., Dyson, H., Wilson, I.A., Wright, P.E.~~		===Solution NMR structure of Kaiso zinc finger DNA binding domain in complex with Kaiso binding site DNA===

	~~Description~~: ~~Solution NMR structure of Kaiso zinc finger DNA~~ binding ~~domain in~~ complex ~~with Kaiso binding site DNA~~
			==About this Structure==
			[[2lt7]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LT7 OCA].
			[[Category: Homo sapiens]]
			[[Category: Buck-Koehntop, B A.]]
			[[Category: Dyson, H.]]
			[[Category: Ekiert, D C.]]
			[[Category: Martinez-Yamout, M A.]]
			[[Category: Stanfield, R L.]]
			[[Category: Wilson, I A.]]
			[[Category: Wright, P E.]]
			[[Category: Double helix]]
			[[Category: Metal binding protein-dna complex]]
			[[Category: Zinc finger]]