2ltd - Revision history

OCA at 06:57, 1 May 2024

2024-05-01T06:57:17Z

← Older revision		Revision as of 06:57, 1 May 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ltd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._lactis_Il1403 Lactococcus lactis subsp. lactis Il1403]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LTD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LTD FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ltd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._lactis_Il1403 Lactococcus lactis subsp. lactis Il1403]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LTD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LTD FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ltd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ltd OCA], [https://pdbe.org/2ltd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ltd RCSB], [https://www.ebi.ac.uk/pdbsum/2ltd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ltd ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ltd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ltd OCA], [https://pdbe.org/2ltd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ltd RCSB], [https://www.ebi.ac.uk/pdbsum/2ltd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ltd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/Q9CIP3_LACLA Q9CIP3_LACLA]		[https://www.uniprot.org/uniprot/Q9CIP3_LACLA Q9CIP3_LACLA]
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Single-stranded DNA (ssDNA) binding proteins are important in basal metabolic pathways for gene transcription, recombination, DNA repair and replication in all domains of life. Their main cellular role is to stabilize melted duplex DNA and protect genomic DNA from degradation. We have uncovered the molecular function of protein domain family domain of unknown function DUF2128 (PF09901) as a novel ssDNA binding domain. This bacterial domain strongly associates into a dimer and presents a highly positively charged surface that is consistent with its function in non-specific ssDNA binding. Lactococcus lactis YdbC is a representative of DUF2128. The solution NMR structures of the 20 kDa apo-YdbC dimer and YdbC:dT(19)G(1) complex were determined. The ssDNA-binding energetics to YdbC were characterized by isothermal titration calorimetry. YdbC shows comparable nanomolar affinities for pyrimidine and mixed oligonucleotides, and the affinity is sufficiently strong to disrupt duplex DNA. In addition, YdbC binds with lower affinity to ssRNA, making it a versatile nucleic acid-binding domain. The DUF2128 family is related to the eukaryotic nuclear protein positive cofactor 4 (PC4) family and to the PUR family both by fold similarity and molecular function.

	Structures of apo- and ssDNA-bound YdbC from Lactococcus lactis uncover the function of protein domain family DUF2128 and expand the single-stranded DNA-binding domain proteome.,Rossi P, Barbieri CM, Aramini JM, Bini E, Lee HW, Janjua H, Xiao R, Acton TB, Montelione GT Nucleic Acids Res. 2013 Jan 8. PMID:23303792<ref>PMID:23303792</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2ltd" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 13:09, 22 February 2023

2023-02-22T13:09:24Z

← Older revision		Revision as of 13:09, 22 February 2023
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	==Solution NMR Structure of apo YdbC from Lactococcus lactis, Northeast Structural Genomics Consortium (NESG) Target KR150==		==Solution NMR Structure of apo YdbC from Lactococcus lactis, Northeast Structural Genomics Consortium (NESG) Target KR150==
	<StructureSection load='2ltd' size='340' side='right'caption='[[2ltd~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>		<StructureSection load='2ltd' size='340' side='right'caption='[[2ltd]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ltd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lacla Lacla~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LTD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LTD FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ltd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._lactis_Il1403 Lactococcus lactis subsp. lactis Il1403]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LTD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LTD FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">L114363, LL0313, ydbC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272623 LACLA])</td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ltd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ltd OCA], [https://pdbe.org/2ltd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ltd RCSB], [https://www.ebi.ac.uk/pdbsum/2ltd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ltd ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ltd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ltd OCA], [https://pdbe.org/2ltd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ltd RCSB], [https://www.ebi.ac.uk/pdbsum/2ltd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ltd ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/Q9CIP3_LACLA Q9CIP3_LACLA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Lacla~~]]		[[Category: Lactococcus lactis subsp. lactis Il1403]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Acton~~, T B~~]]		[[Category: Acton TB]]
	[[Category: Aramini~~, J M~~]]		[[Category: Aramini JM]]
	[[Category: Barbieri~~, C M~~]]		[[Category: Barbieri CM]]
	[[Category: Bini, E]]		[[Category: Bini E]]
	[[Category: Ciccosanti, C]]		[[Category: Ciccosanti C]]
	[[Category: Everett~~, J K~~]]		[[Category: Everett JK]]
	[[Category: Janjua, H]]		[[Category: Janjua H]]
	[[Category: Lee, H]]		[[Category: Lee H]]
	[[Category: Montelione~~, G T]]~~		[[Category: Montelione GT]]
	~~[[Category: Structural genomic~~]]		[[Category: Rossi P]]
	[[Category: Rossi, P]]		[[Category: Wang H]]
	[[Category: Wang, H]]		[[Category: Xiao R]]
	[[Category: Xiao, R]]
	~~[[Category: Nesg]]~~
	~~[[Category: PSI, Protein structure initiative]]~~
	~~[[Category: Psi-biology]]~~
	~~[[Category: Unknown function~~]]

OCA at 10:52, 19 May 2021

2021-05-19T10:52:49Z

← Older revision		Revision as of 10:52, 19 May 2021
Line 1:		Line 1:

	==Solution NMR Structure of apo YdbC from Lactococcus lactis, Northeast Structural Genomics Consortium (NESG) Target KR150==		==Solution NMR Structure of apo YdbC from Lactococcus lactis, Northeast Structural Genomics Consortium (NESG) Target KR150==
	<StructureSection load='2ltd' size='340' side='right' caption='[[2ltd]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2ltd' size='340' side='right'caption='[[2ltd]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ltd]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lacla Lacla]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LTD OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2LTD FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ltd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lacla Lacla]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LTD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LTD FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">L114363, LL0313, ydbC ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272623 LACLA])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">L114363, LL0313, ydbC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272623 LACLA])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ltd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ltd OCA], [~~http~~://pdbe.org/2ltd PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ltd RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ltd PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ltd ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ltd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ltd OCA], [https://pdbe.org/2ltd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ltd RCSB], [https://www.ebi.ac.uk/pdbsum/2ltd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ltd ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Lacla]]		[[Category: Lacla]]
			[[Category: Large Structures]]
	[[Category: Acton, T B]]		[[Category: Acton, T B]]
	[[Category: Aramini, J M]]		[[Category: Aramini, J M]]

OCA at 19:36, 1 August 2018

2018-08-01T19:36:47Z

← Older revision		Revision as of 19:36, 1 August 2018
Line 1:		Line 1:

	==Solution NMR Structure of apo YdbC from Lactococcus lactis, Northeast Structural Genomics Consortium (NESG) Target KR150==		==Solution NMR Structure of apo YdbC from Lactococcus lactis, Northeast Structural Genomics Consortium (NESG) Target KR150==
	<StructureSection load='2ltd' size='340' side='right' caption='[[2ltd]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2ltd' size='340' side='right' caption='[[2ltd]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2ltd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lacla Lacla]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LTD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LTD FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ltd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lacla Lacla]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LTD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LTD FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">L114363, LL0313, ydbC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272623 LACLA])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">L114363, LL0313, ydbC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272623 LACLA])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ltd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ltd OCA], [http://pdbe.org/2ltd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ltd RCSB], [http://www.ebi.ac.uk/pdbsum/2ltd PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ltd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ltd OCA], [http://pdbe.org/2ltd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ltd RCSB], [http://www.ebi.ac.uk/pdbsum/2ltd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ltd ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 02:31, 10 September 2015

2015-09-10T02:31:41Z

← Older revision		Revision as of 02:31, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2ltd' size='340' side='right' caption='[[2ltd]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2ltd' size='340' side='right' caption='[[2ltd]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ltd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Lactococcus_lactis_subsp._lactis_il1403 Lactococcus lactis subsp. lactis il1403~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LTD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LTD FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ltd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lacla Lacla]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LTD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LTD FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">L114363, LL0313, ydbC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272623 ~~Lactococcus lactis subsp. lactis Il1403~~])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">L114363, LL0313, ydbC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272623 LACLA])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ltd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ltd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ltd RCSB], [http://www.ebi.ac.uk/pdbsum/2ltd PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ltd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ltd OCA], [http://pdbe.org/2ltd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ltd RCSB], [http://www.ebi.ac.uk/pdbsum/2ltd PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 14:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2ltd" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Lactococcus lactis subsp. lactis il1403~~]]		[[Category: Lacla]]
	[[Category: Acton, T B]]		[[Category: Acton, T B]]
	[[Category: Aramini, J M]]		[[Category: Aramini, J M]]

OCA at 10:23, 25 January 2015

2015-01-25T10:23:21Z

← Older revision		Revision as of 10:23, 25 January 2015
Line 19:		Line 19:
	</StructureSection>		</StructureSection>
	[[Category: Lactococcus lactis subsp. lactis il1403]]		[[Category: Lactococcus lactis subsp. lactis il1403]]
	[[Category: Acton, T B.]]		[[Category: Acton, T B]]
	[[Category: Aramini, J M.]]		[[Category: Aramini, J M]]
	[[Category: Barbieri, C M.]]		[[Category: Barbieri, C M]]
	[[Category: Bini, E.]]		[[Category: Bini, E]]
	[[Category: Ciccosanti, C.]]		[[Category: Ciccosanti, C]]
	[[Category: Everett, J K.]]		[[Category: Everett, J K]]
	[[Category: Janjua, H.]]		[[Category: Janjua, H]]
	[[Category: Lee, H.]]		[[Category: Lee, H]]
	[[Category: Montelione, G T.]]		[[Category: Montelione, G T]]
	[[Category: ~~NESG, Northeast~~ Structural ~~Genomics Consortium.~~]]		[[Category: Structural genomic]]
	[[Category: Rossi, P.]]		[[Category: Rossi, P]]
	[[Category: Wang, H.]]		[[Category: Wang, H]]
	[[Category: Xiao, R.]]		[[Category: Xiao, R]]
	[[Category: Nesg]]		[[Category: Nesg]]
	[[Category: ~~Northeast structural genomics consortium]]~~		[[Category: PSI, Protein structure initiative]]
	~~[[Category:~~ Protein structure initiative]]
	[[Category: Psi-biology]]		[[Category: Psi-biology]]
	~~[[Category: Structural genomic]]~~
	[[Category: Unknown function]]		[[Category: Unknown function]]

OCA at 09:30, 5 November 2014

2014-11-05T09:30:06Z

← Older revision		Revision as of 09:30, 5 November 2014
Line 1:		Line 1:
	[[~~Image~~:2ltd.~~png\|left\|200px~~]]		==Solution NMR Structure of apo YdbC from Lactococcus lactis, Northeast Structural Genomics Consortium (NESG) Target KR150==
			<StructureSection load='2ltd' size='340' side='right' caption='[[2ltd]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2ltd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._lactis_il1403 Lactococcus lactis subsp. lactis il1403]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LTD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LTD FirstGlance]. <br>
			</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">L114363, LL0313, ydbC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272623 Lactococcus lactis subsp. lactis Il1403])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ltd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ltd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ltd RCSB], [http://www.ebi.ac.uk/pdbsum/2ltd PDBsum]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Single-stranded DNA (ssDNA) binding proteins are important in basal metabolic pathways for gene transcription, recombination, DNA repair and replication in all domains of life. Their main cellular role is to stabilize melted duplex DNA and protect genomic DNA from degradation. We have uncovered the molecular function of protein domain family domain of unknown function DUF2128 (PF09901) as a novel ssDNA binding domain. This bacterial domain strongly associates into a dimer and presents a highly positively charged surface that is consistent with its function in non-specific ssDNA binding. Lactococcus lactis YdbC is a representative of DUF2128. The solution NMR structures of the 20 kDa apo-YdbC dimer and YdbC:dT(19)G(1) complex were determined. The ssDNA-binding energetics to YdbC were characterized by isothermal titration calorimetry. YdbC shows comparable nanomolar affinities for pyrimidine and mixed oligonucleotides, and the affinity is sufficiently strong to disrupt duplex DNA. In addition, YdbC binds with lower affinity to ssRNA, making it a versatile nucleic acid-binding domain. The DUF2128 family is related to the eukaryotic nuclear protein positive cofactor 4 (PC4) family and to the PUR family both by fold similarity and molecular function.

	~~{{STRUCTURE_2ltd\| PDB=2ltd \| SCENE= }}~~		Structures of apo- and ssDNA-bound YdbC from Lactococcus lactis uncover the function of protein domain family DUF2128 and expand the single-stranded DNA-binding domain proteome.,Rossi P, Barbieri CM, Aramini JM, Bini E, Lee HW, Janjua H, Xiao R, Acton TB, Montelione GT Nucleic Acids Res. 2013 Jan 8. PMID:23303792<ref>PMID:23303792</ref>

	~~===Solution NMR Structure~~ of ~~apo YdbC from Lactococcus lactis, Northeast Structural Genomics Consortium (NESG) Target KR150===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_23303792}}~~		== References ==
			<references/>
	==~~About this Structure~~==		__TOC__
	~~[[2ltd]] is a 2 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Lactococcus_lactis_subsp._lactis_il1403 Lactococcus lactis subsp. lactis il1403]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LTD OCA].		</StructureSection>
	[[Category: Lactococcus lactis subsp. lactis il1403]]		[[Category: Lactococcus lactis subsp. lactis il1403]]
	[[Category: Acton, T B.]]		[[Category: Acton, T B.]]

OCA at 12:28, 30 January 2013

2013-01-30T12:28:25Z

← Older revision		Revision as of 12:28, 30 January 2013
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	===Solution NMR Structure of apo YdbC from Lactococcus lactis, Northeast Structural Genomics Consortium (NESG) Target KR150===		===Solution NMR Structure of apo YdbC from Lactococcus lactis, Northeast Structural Genomics Consortium (NESG) Target KR150===

			{{ABSTRACT_PUBMED_23303792}}

	==About this Structure==		==About this Structure==

OCA at 09:16, 16 January 2013

2013-01-16T09:16:55Z

← Older revision		Revision as of 09:16, 16 January 2013
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	[[Image:2ltd.~~jpg~~\|left\|200px]]		[[Image:2ltd.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2ltd", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2ltd\| PDB=2ltd \| SCENE= }}		{{STRUCTURE_2ltd\| PDB=2ltd \| SCENE= }}

OCA at 07:48, 6 June 2012

2012-06-06T07:48:02Z

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:2ltd.jpg|left|200px]]
-The entry 2ltd is ON HOLD
+<!--
-Authors: Rossi, P., Barbieri, C.M., Aramini, J.A., Bini, E., Lee, H., Janjua, H., Ciccosanti, C., Wang, H., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Northeast Structural Genomics Consortium (NESG)
+===Solution NMR Structure of apo YdbC from Lactococcus lactis, Northeast Structural Genomics Consortium (NESG) Target KR150===
-Description: Solution NMR Structure of apo YdbC from Lactococcus lactis, Northeast Structural Genomics Consortium (NESG) Target KR150